Optimization of purification of polysaccharides from the Inonotus obliquus using DEAE-52 cellulose static method

In order to purify the polysaccharides efficiently and rapidly from the fruiting body of Inonotus obliquus,the DEAE-52 cellulose static absorption and desorption method was used. The polysaccharide concentration,adsorption time,adsorption temperature,rotate speed,elution time and eluent volume that influence the static adsorption and desorption of polysaccharide were selected and optimized. The optimal experimental conditions for static adsorption were determined as 40 mg/mL,90 min,30 ℃,120 r/min for sample concentration,adsorption time,adsorption temperature,and rotate speed,respectively. And the desorption effect was the best when eluted with 12 times volume of deionized water for 90 min and eluted with 11 times the volume of 0.2 mol/L NaCl solution for 60 min. Under the optimized conditions,the concentration of polysaccharide in deionized water and 0.2 mol/L NaCl elution polysaccharides were 0.56 mg/mL and 0.38 mg/mL,respectively. And,the two polysaccharides that were eluted were homogeneous distribution of polysaccharide components,which was consistent with the separation of DEAE-52 cellulose column chromatography in the previous experiment. Therefore,it is feasible to isolate and purify the polysaccharides of Inonotus obliquus by DEAE-52 cellulose static method.