Study on total plate count detection in acid beverage by flow cytometry

ZHANG Lan; XU Hong; WENG Wen-chuan; LING Li; XIAN Yu-yin; YANG Ming-yan; PAN Si-wei; HUANG Zhuo-zhi

Volume 39 Issue 4

Feb. 2018

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Science and Technology of Food Industry > 2018 > 39(4): 235-239.

ZHANG Lan, XU Hong, WENG Wen-chuan, LING Li, XIAN Yu-yin, YANG Ming-yan, PAN Si-wei, HUANG Zhuo-zhi. Study on total plate count detection in acid beverage by flow cytometry[J]. Science and Technology of Food Industry, 2018, 39(4): 235-239.

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Study on total plate count detection in acid beverage by flow cytometry

1. Danone(China) Food & Beverage Co., Ltd., Zhongshan 528415, China;
2. Guangdong Inspection and Quarantine Technology Center, Guangdong Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food, Guanzhou 510000, China

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Received Date: August 03, 2017
Available Online: December 25, 2020

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Abstract

Abstract

Compared and analyzed with pour plate method and membrane filtration method,flow cytometer(FCM)was used to determine total plate count(TPC)for establishing a FCM method in rapid detection of TPC in acid beverage(pH＜4.6). 13 strains were selected to conduct artificial add experiment. The results showed that substrate and concentration had great influence on FCM method,some bacteria in acid beverage could not be detected by FCM method directly,which need to be filtrated on membrane and pre-enriched in neutral TSB broth at 36 ℃ for 22 h,then detected by FCM and pour plate method,results were at the same order of magnitude. The official AOAC analysis method was used to do the statistics analysis on qualitative result of 13 strains in acid beverage with different concentration detected by membrane filtration method and FCM method after pre-enrichment for 22 h,X²<3.84 and false negative rate was 1.9%<2%,false positive rate was 4.2%<9.6%,which showed that membrane filtration method could be replaced by FCM method for TPC qualitative detection in acid beverage,then used for products quick release.
- acid beverage,
- FCM,
- TPC,
- pour plate method,
- membrane filtration method

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