Safety and Probiotic Evaluation of <i>Lactiplantibacillus plantarum</i> CHEN1 and Its Metabolic Analysis of Soymilk Oligosaccharides

CHEN Zhina; CAO Shiyi; YIN Linlin; SHAO Mengyuan; YE Tao; XU Zhangrui; HUANG Xiaochen

doi:10.13386/j.issn1002-0306.2024110347

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CHEN Zhina, CAO Shiyi, YIN Linlin, et al. Safety and Probiotic Evaluation of Lactiplantibacillus plantarum CHEN1 and Its Metabolic Analysis of Soymilk Oligosaccharides[J]. Science and Technology of Food Industry, 2025, 46(10): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024110347.

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Safety and Probiotic Evaluation of Lactiplantibacillus plantarum CHEN1 and Its Metabolic Analysis of Soymilk Oligosaccharides

1.
School of Biological Engineering, Huainan Normal University, Huainan 232038, China
2.
School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China

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Received Date: June 11, 2024
Available Online: March 19, 2025

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Abstract

Abstract

Lactiplantibacillus plantarum was an important source of probiotics. This study aimed to evaluate the safety, probiotic properties, and metabolic potential of carbohydrate-active enzymes of Lactiplantibacillus plantarum CHEN1. The genetic characteristics of the strain were analyzed through genome sequencing and functional annotation. Phenotypic experiments, including hemolytic activity assays, detection of harmful metabolites, antibiotic susceptibility testing, tolerance experiments, hydrophobicity, and autoaggregation assays were conducted to evaluate its safety and probiotic potential. Furthermore, its metabolic mechanism for soymilk oligosaccharides was elucidated. The results showed that the strain's genome lacked genes associated with harmful metabolism, such as nitroreductase and trypsin. Hemolysis tests and harmful metabolite detection were negative. Antibiotic susceptibility testing showed sensitivity to β-lactam and chloramphenicol antibiotics, with low transferability of resistance genes, indicating high safety. The strain exhibited a survival rate of 22.47% after incubation at pH2.0 for 2 hours, 33.80% in 0.3% bile salts, and 73.32% in simulated gastrointestinal fluid. The hydrophobicity and autoaggregation rates were 41.83% and 13.47%, respectively, demonstrating good probiotic characteristics. Genome functional annotation revealed that the strain possessed complete metabolic pathways for raffinose and stachyose. This was further validated through soymilk fermentation experiments. After fermentation, the stachyose content in soymilk decreased from 1043.83 mg/L to 536.01 mg/L, and the raffinose content decreased from 270.69 mg/L to 134.69 mg/L, indicating that CHEN1 has a strong metabolic capacity for these two sugars and the potential to degrade flatulence-inducing factors in soymilk and improve digestibility. In conclusion, L. plantarum CHEN1 exhibits potential safety and probiotic properties and can be used as a functional strain for the fermentation of plant-based foods.
- Lactiplantibacillus plantarum,
- metabolic analysis,
- safety,
- probiotics,
- soymilk oligosaccharides

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