GAO Ruohan, MA Nan, YANG Mingzhe, et al. Optimization of the Fermentation Enrichment Method for Oat Non-starch Polysaccharides and Study of Their Immunostimulatory Activity[J]. Science and Technology of Food Industry, 2025, 46(10): 21−32. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024090061.
Citation: GAO Ruohan, MA Nan, YANG Mingzhe, et al. Optimization of the Fermentation Enrichment Method for Oat Non-starch Polysaccharides and Study of Their Immunostimulatory Activity[J]. Science and Technology of Food Industry, 2025, 46(10): 21−32. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024090061.

Optimization of the Fermentation Enrichment Method for Oat Non-starch Polysaccharides and Study of Their Immunostimulatory Activity

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  • Received Date: September 04, 2024
  • Available Online: March 11, 2025
  • In order to enhance the yield of oat non-starch polysaccharide (ONSP), the extraction process was optimized and its immunological activity was investigated. This study employed microbial fermentation-assisted extraction, optimizing the process for oat non-starch polysaccharide (ONSP1) using a one-way response surface methodology. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the structural characteristics of ONSP. Additionally, the immunological activity of ONSP was investigated and compared with that of oat non-starch polysaccharide (ONSP2) extracted through the conventional hot water method. The results demonstrated that the optimal extraction process for ONSP1 was achieved with a 5% of the inoculum and fermentation at 34 ℃ for 26 h, resulting in a yield of ONSP1 at 8.60%±0.04%. Both ONSP1 and ONSP2 were composed of glucose, arabinose, and xylose, with the contents of arabinose and xylose of ONSP1 were significantly higher than those of ONSP2 (P<0.05). Despite the lower molecular weight of ONSP1 (47.2 kDa) compared to ONSP2 (53.2 kDa), ONSP1 had a significantly higher β-glucan content (P<0.05). Furthermore, both ONSP1 and ONSP2 demonstrated the capacity to promote the proliferation of RAW264.7 cells, with ONSP1 exhibiting a more pronounced immune-stimulating activity. In conclusion, microbial fermentation enrichment resulted in oat non-starch polysaccharides with a higher β-glucan content and stronger immunostimulatory activity. This study provides new insights for further investigation into the bioimmunological activities and structural relationships of oat non-starch polysaccharides.
  • [1]
    HU H, LIN H, XIAO L, et al. Impact of native form oat β-glucan on the physical and starch digestive properties of whole oat bread[J]. Foods,2022,11(17):2622. doi: 10.3390/foods11172622
    [2]
    WANG X, LI L, YUAN G, et al. Interleukin (IL)-22 in common carp (Cyprinus carpio L.):Immune modulation, antibacterial defense, and activation of the JAK-STAT signaling pathway[J]. Fish & Shellfish Immunology,2022,131:796−808.
    [3]
    ZHANG J, WEN C, CHEN M, et al. Antioxidant activities of Sagittaria sagittifolia L. polysaccharides with subcritical water extraction[J]. International Journal of Biological Macromolecules,2019,134:172−179. doi: 10.1016/j.ijbiomac.2019.05.047
    [4]
    ZHANG J, WEN C, GU J, et al. Effects of subcritical water extraction microenvironment on the structure and biological activities of polysaccharides from Lentinus edodes[J]. International Journal of Biological Macromolecules,2019,123:1002−1011. doi: 10.1016/j.ijbiomac.2018.11.194
    [5]
    LIU Y, SUN Y, HUANG G. Preparation and antioxidant activities of important traditional plant polysaccharides[J]. International Journal of Biological Macromolecules,2018,111:780−786. doi: 10.1016/j.ijbiomac.2018.01.086
    [6]
    ZHANG J, WEN C, QIN W, et al. Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes[J]. International Journal of Biological Macromolecules,2018,118:2269−2277. doi: 10.1016/j.ijbiomac.2018.07.098
    [7]
    LI D, CHEN M, MENG X, et al. Extraction, purification, structural characteristics, bioactivity and potential applications of polysaccharides from Avena sativa L:A review[J]. International Journal of Biological Macromolecules,2024,265:130891. doi: 10.1016/j.ijbiomac.2024.130891
    [8]
    KOVITVADHI A, CHUNDANG P, TIRAWATTANAWANICH C, et al. Effects of dietary supplementation with different levels and molecular weights of fungal β-glucan on performances, health and meat quality in broilers[J]. Asian-Australasian Journal of Animal Sciences,2019,32(10):1548−1557. doi: 10.5713/ajas.18.0927
    [9]
    JANSSEN F, WOUTERS A G B, MEEUS Y, et al. The role of non-starch polysaccharides in determining the air-water interfacial properties of wheat, rye, and oat dough liquor constituents[J]. Food Hydrocolloids,2020,105:105771. doi: 10.1016/j.foodhyd.2020.105771
    [10]
    TIAN L, SCHOLTE J, SCHEURINK A J W, et al. Effect of oat and soybean rich in distinct non-starch polysaccharides on fermentation, appetite regulation and fat accumulation in rat[J]. International Journal of Biological Macromolecules,2019,140:515−521. doi: 10.1016/j.ijbiomac.2019.08.032
    [11]
    AKTAS-AKYILDIZ E, SIBAKOV J, NAPPA M, et al. Extraction of soluble β-glucan from oat and barley fractions:Process efficiency and dispersion stability[J]. Journal of Cereal Science,2018,81:60−68. doi: 10.1016/j.jcs.2018.03.007
    [12]
    HUSSAIN A, BOSE S, WANG J H, et al. Fermentation, a feasible strategy for enhancing bioactivity of herbal medicines[J]. Food Research International,2016,81:1−16. doi: 10.1016/j.foodres.2015.12.026
    [13]
    PESSÔA M G, VESPERMANN K A C, PAULINO B N, et al. Newly isolated microorganisms with potential application in biotechnology[J]. Biotechnology Advances,2019,37(2):319−339. doi: 10.1016/j.biotechadv.2019.01.007
    [14]
    SUN W, LABRECHE F, KOU X H, et al. Efficient extraction, physiochemical, rheological properties, and antioxidant activities of polysaccharides from Armeniaca vulgaris Lam[J]. Process Biochemistry,2022,118:360−369. doi: 10.1016/j.procbio.2022.04.032
    [15]
    YUAN S, XU C, XIA J, et al. Extraction of polysaccharides from Codonopsis pilosula by fermentation with response surface methodology[J]. Food Science & Nutrition,2020,8(12):6660−6669.
    [16]
    WANG Q, HAO L, ZHANG A, et al. Extraction and characterization of polysaccharides from Schisandra sphenanthera fruit by Lactobacillus plantarum CICC 23121-assisted fermentation[J]. International Journal of Biological Macromolecules,2024,259:129135. doi: 10.1016/j.ijbiomac.2023.129135
    [17]
    WANG H, WANG J, MUJUMDAR A S, et al. Effects of postharvest ripening on physicochemical properties, microstructure, cell wall polysaccharides contents (pectin, hemicellulose, cellulose) and nanostructure of kiwifruit (Actinidia deliciosa)[J]. Food Hydrocolloids,2021,118:106808. doi: 10.1016/j.foodhyd.2021.106808
    [18]
    WANG X, HU K, CHEN Y, et al. Effect of Lactiplantibacillus plantarum fermentation on the physicochemical, antioxidant activity and immunomodulatory ability of polysaccharides from Lvjian okra[J]. International Journal of Biological Macromolecules,2024,257:128649. doi: 10.1016/j.ijbiomac.2023.128649
    [19]
    WU J, JIN S, WU S, et al. Effect of filamentous fungi fermentation on the extractability and physicochemical properties of β-glucan in oat bran[J]. Food Chemistry,2018,254:122−128. doi: 10.1016/j.foodchem.2018.01.158
    [20]
    CLIMOVA A, IBRAHIM M N G, SALAMAHINA A, et al. Application of extracted β-glucan from oat for β-carotene encapsulation[J]. Journal of Food Science and Technology,2021,58(7):2641−2650. doi: 10.1007/s13197-020-04770-2
    [21]
    PONOMAREVA S A, GOLOVCHENKO V V, PATOVA O A, et al. Comparative analysis of the spectrophotometric methods of the protein amount determination in the pectic polysaccharide samples[J]. Russian Journal of Bioorganic Chemistry,2015,41(2):133−139. doi: 10.1134/S1068162015020119
    [22]
    YUE F, ZHANG J, XU J, et al. Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method[J]. Frontiers in Nutrition,2022,9:963318. doi: 10.3389/fnut.2022.963318
    [23]
    PANG X, WANG H, GUAN C, et al. Impact of molecular weight variations in dendrobium officinale polysaccharides on antioxidant activity and anti-obesity in caenorhabditis elegans[J]. Foods,2024,13(7):1040. doi: 10.3390/foods13071040
    [24]
    GALERMO A G, NANDITA E, BARBOZA M, et al. Liquid chromatography–tandem mass spectrometry approach for determining glycosidic linkages[J]. Analytical Chemistry,2018,90(21):13073−13080. doi: 10.1021/acs.analchem.8b04124
    [25]
    ERNST L, WERNER A, WEFERS D. Influence of ultrasonication and hydrolysis conditions in methylation analysis of bacterial homoexopolysaccharides[J]. Carbohydrate Polymers,2023,308:120643. doi: 10.1016/j.carbpol.2023.120643
    [26]
    YELITHAO K, SURAYOT U, LEE C, et al. Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune[J]. Carbohydrate Polymers,2019,218:37−45. doi: 10.1016/j.carbpol.2019.04.057
    [27]
    SURAYOT U, HUN L J, KANONGNUCH C, et al. Structural characterization of sulfated arabinans extracted from Cladophora glomerata Kützing and their macrophage activation[J]. Bioscience, Biotechnology, and Biochemistry,2016,80(5):972−982. doi: 10.1080/09168451.2015.1132149
    [28]
    EOM S J, KIM T W, KIM S, et al. Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae[J]. International Journal of Biological Macromolecules,2021,188:245−252. doi: 10.1016/j.ijbiomac.2021.08.044
    [29]
    WANG Z, SUN Q, FANG J, et al. The anti-aging activity of Lycium barbarum polysaccharide extracted by yeast fermentation:In vivo and in vitro studies[J]. International Journal of Biological Macromolecules,2022,209:2032−2041. doi: 10.1016/j.ijbiomac.2022.04.184
    [30]
    MA N, LI R, YOU S, et al. Fermentation enrichment, structural characterization and immunostimulatory effects of β-glucan from Quinoa[J]. International Journal of Biological Macromolecules,2024,267:131162. doi: 10.1016/j.ijbiomac.2024.131162
    [31]
    LI L, WANG L, FAN W, et al. The application of fermentation technology in traditional chinese medicine:A review[J]. The American Journal of Chinese Medicine,2020,48(4):899−921. doi: 10.1142/S0192415X20500433
    [32]
    茆鑫, 郑剑斌, 李广耀, 等. 响应曲面法优化刺五加-五味子混菌发酵工艺的研究[J]. 食品科技,2023,48(9):57−64. [MAO X, ZHENG J B, LI G Y, et al. Response surface methodology for optimizing the fermentation process of acanthopanax senticosus and schisandra chinensis with mixed bacteria[J]. Food Science and Technology,2023,48(9):57−64.]

    MAO X, ZHENG J B, LI G Y, et al. Response surface methodology for optimizing the fermentation process of acanthopanax senticosus and schisandra chinensis with mixed bacteria[J]. Food Science and Technology, 2023, 48(9): 57−64.
    [33]
    CAO R A , MA N, SUBRAMANIAN P , et al. Structural elucidation and immunostimulatory activities of quinoa non-starch polysaccharide before and after deproteinization[J]. Journal of Polymers and the Environment, 2021, 30(6):11−13.
    [34]
    高泽鑫. 苏云金芽孢杆菌的多糖发酵、结构解析及其功能研究[D]. 无锡:江南大学, 2024. [GAO Z X. Study on polysaccharide fermentation, structural elucidation and function of Bacillus thuringiensis[D]. Wuxi:Jiangnan University, 2024.]

    GAO Z X. Study on polysaccharide fermentation, structural elucidation and function of Bacillus thuringiensis[D]. Wuxi: Jiangnan University, 2024.
    [35]
    CAO R A, MA N, PALANISAMY S, et al. Structural elucidation and immunostimulatory activities of quinoa non-starch polysaccharide before and after deproteinization[J]. Journal of Polymers and the Environment,2022,30(6):2291−2303. doi: 10.1007/s10924-021-02335-8
    [36]
    XIE J, REN Y, XIAO Y, et al. Interactions between tapioca starch and Mesona chinensis polysaccharide:Effects of urea and NaCl[J]. Food Hydrocolloids,2021,111:106268. doi: 10.1016/j.foodhyd.2020.106268
    [37]
    PÉREZ-BASSART Z, FABRA M J, MARTÍNEZ-ABAD A, et al. Compositional differences of β-glucan-rich extracts from three relevant mushrooms obtained through a sequential extraction protocol[J]. Food Chemistry,2023,402:134207. doi: 10.1016/j.foodchem.2022.134207
    [38]
    LIU H, LI Y, YOU M, et al. Comparison of physicochemical properties of β-glucans extracted from hull-less barley bran by different methods[J]. International Journal of Biological Macromolecules,2021,182:1192−1199. doi: 10.1016/j.ijbiomac.2021.05.043
    [39]
    ZHAO Y, WANG N, PANG S F, et al. In-situ micro-FTIR spectroscopic observation on the hydration process of Poria cocos[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,164:61−66. doi: 10.1016/j.saa.2016.03.039
    [40]
    CHEN J, LI L, ZHOU X, et al. Structural characterization and α-glucosidase inhibitory activity of polysaccharides extracted from Chinese traditional medicine Huidouba[J]. International Journal of Biological Macromolecules,2018,117:815−819. doi: 10.1016/j.ijbiomac.2018.05.192
    [41]
    SRINIVASAN A, EKAMBARAM S P, PERUMAL S S, et al. Chemical characterization and immunostimulatory activity of phenolic acid bound arabinoxylans derived from foxtail and barnyard millets[J]. Journal of Food Biochemistry,2020,44(2):e13116.
    [42]
    AZMI A F M N, MUSTAFA S, HASHIM D M D, et al. Prebiotic activity of polysaccharides extracted from Gigantochloa Levis (Buluh beting) shoots[J]. Molecules,2012,17(2):1635−1651. doi: 10.3390/molecules17021635
    [43]
    LEE J, LI C, SURAYOT U, et al. Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome[J]. International Journal of Biological Macromolecules,2018,120:1726−1733. doi: 10.1016/j.ijbiomac.2018.09.138
    [44]
    YANG B, ZHAO M, SHI J, et al. Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp[J]. Food Chemistry,2008,106(2):685−690. doi: 10.1016/j.foodchem.2007.06.031
    [45]
    SINGH R, DE S, BELKHEIR A. Avena sativa (Oat), a potential neutraceutical and therapeutic agent:An overview[J]. Critical Reviews in Food Science and Nutrition,2013,53(2):126−144. doi: 10.1080/10408398.2010.526725
    [46]
    HOU C, CHEN L, YANG L, et al. An insight into anti-inflammatory effects of natural polysaccharides[J]. International Journal of Biological Macromolecules,2020,153:248−255. doi: 10.1016/j.ijbiomac.2020.02.315
    [47]
    TAKAHASHI S, SAKAMAKI M, FERDOUSI F, et al. Ethanol extract of aurantiochytrium mangrovei 18w-13a strain possesses anti-inflammatory effects on murine macrophage RAW264 cells[J]. Frontiers in Physiology,2018,9:1205. doi: 10.3389/fphys.2018.01205
    [48]
    SOOMRO S, MESAIK M A, SHAHEEN F, et al. Inhibitory effects of myrtucommuacetalone 1 (MCA-1) from myrtus communis on inflammatory response in mouse macrophages[J]. Molecules,2019,25(1):13. doi: 10.3390/molecules25010013
    [49]
    WANG T, LUO S, QIN H, et al. Hsp90 inhibition renders iNOS aggregation and the clearance of iNOS aggregates by proteasomes requires SPSB2[J]. Free Radical Biology and Medicine,2018,117:90−98. doi: 10.1016/j.freeradbiomed.2018.01.021
    [50]
    KORYTOWSKI W, BAZAK J, FAHEY J, et al. Beneficial effects of inhibiting iNOS activity versus iNOS transcription in anti-tumor photodynamic therapy[J]. Free Radical Biology and Medicine,2018,120:S155.
    [51]
    ARAMSANGTIENCHAI P, RAKSACHUE W, PECHROJ S, et al. The immunomodulatory activity of levan in RAW264.7 macrophage varies with its molecular weights[J]. Food Bioscience,2023,53:102721. doi: 10.1016/j.fbio.2023.102721
    [52]
    WANG X, STORSLEY J, THANDAPILLY S J, et al. Effects of processing, cultivar, and environment on the physicochemical properties of oat β-glucan[J]. Cereal Chemistry,2016,93(4):402−408. doi: 10.1094/CCHEM-12-15-0245-R

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