Preparation, Structure and <i>in Vitro</i> Activity Analysis of Selenopolysaccharide from Auricularia

SHEN Qin; LU Lele; PAN Piao; YUE Shibo; CAO Dongmei

doi:10.13386/j.issn1002-0306.2024070056

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SHEN Qin, LU Lele, PAN Piao, et al. Preparation, Structure and in Vitro Activity Analysis of Selenopolysaccharide from Auricularia[J]. Science and Technology of Food Industry, 2025, 46(10): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070056.

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Preparation, Structure and in Vitro Activity Analysis of Selenopolysaccharide from Auricularia

SHEN Qin^1,,
LU Lele¹,
PAN Piao¹,
YUE Shibo¹,
CAO Dongmei^{1, 2, 3, 4, ,}

1.
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2.
Mudanjiang Food and Biotechnology Innovation Research Institute, Heilongjiang Bayi Agricultural University, Mudanjiang 157000, China
3.
National Coarse Cereals Engineering Research Center, Daqing 163319, China
4.
Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing 163319, China

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Received Date: July 04, 2024
Available Online: March 13, 2025

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Abstract

Abstract

To advance the research and application of selenopolysaccharides derived from Auriculus, selenopolysaccharides isolated from the fruiting bodies of Auricularia and subjected to structural and functional characterization. Optimal extraction conditions were determined as follows: a solid-liquid ratio of 1:60, extraction temperature of 50 ℃, and extraction time of 3 h, resulting in a polysaccharide yield of 11.62%. The conversion efficiency of selenated Auricularia to inorganic selenium was measured at 88.90%. The spectral analysis of selenium polysaccharides revealed characteristic absorption peaks corresponding to Se=O, Se-O and other functional groups. The surface morphology of selenated Auricularia exhibited a well-organized network structure with minimal alteration in crystal morphology. Additionally, the antioxidant capacity of selenopolysaccharides was significantly (P<0.05) enhanced compared to non-selenized polysaccharides. At a concentration of 5 mg/mL, selenopolysaccharides demonstrated an 18.80% increase in inhibitory activity against α-glucosidase and an 8.30% increase against α-amylase, as well as a cholesterol adsorption rate of 61.40%. These findings provide valuable theoretical and technical insights for the development of Auricularia selenopolysaccharide products.
- Auricularia,
- selenopolysaccharide,
- extraction optimization,
- structural,
- functional characteristics

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References (33)

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