Expression and Characterization of Xyloglucanase from <i>Paenibacillus borealis</i> and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace

TIAN Jiaxin; YANG Hang; ZHAO Yuqi; YAN Qiaojuan; LI Yanxiao; JIANG Zhengqiang

doi:10.13386/j.issn1002-0306.2024060437

Volume 46 Issue 11

May 2025

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Science and Technology of Food Industry > 2025 > 46(11): 142-150. > DOI: 10.13386/j.issn1002-0306.2024060437

TIAN Jiaxin, YANG Hang, ZHAO Yuqi, et al. Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace[J]. Science and Technology of Food Industry, 2025, 46(11): 142−150. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060437.

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Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace

1.
Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2.
College of Engineering, China Agricultural University, Beijing 100083, China
3.
Food Laboratory of Zhongyuan, Luohe 462300, China
4.
College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China

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Received Date: June 30, 2024
Available Online: March 30, 2025

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Abstract

Abstract

Apple pomace (AP) is rich in xyloglucan, serves as a substrate to produce xylogluco-oligosaccharides with various functional activities. The hydrolysis of AP by novel xyloglucanases is conducive to the high-value utilization of AP. In this study, a novel GH74 xyloglucanase gene (PbXEG74B) from Paenibacillus borealis was cloned and expressed in Escherichia coli BL21 (DE3). The enzyme was characterized and further used to hydrolyze AP for xylogluco-oligosaccharides production. It was expressed in E. coli with an expression level of 18.0 U/mL. The optimal conditions of PbXEG74B were pH5.5 and 55 ℃. It showed good stability in the pH range of 4.5~8.5 and temperatures up to 40 ℃. The enzyme hydrolyzed xyloglucan to produce xylogluco-oligosaccharides with degrees of polymerization (DP) of 2~9. AP was pretreated with deep eutectic solvents (DESs) and hydrolyzed by PbXEG74B to produce xylogluco-oligosaccharides. Under optimal conditions, the yield of xylogluco-oligosaccharides with DP 2~9 was 2.79 g/100 g AP, containing 67.0%, 11.5%, and 21.5% of xylogluco-oligosaccharides with DP 2~4, 5~6, and 7~9, respectively. PbXEG74B with good enzymatic and hydrolytic properties shows great potential in the high value utilization of AP.
- Paenibacillus borealis,
- xyloglucanase,
- apple pomace,
- xylogluco-oligosaccharide,
- deep eutectic solvents

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References (41)

References

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