Effects of Saponins of Semen Ziziphi Spinosae on Oxidative Damage of Liver and Kidney and Intestinal Microflora in Lead-exposed Mice

CAO Weidong; LIU Lihong

doi:10.13386/j.issn1002-0306.2024060299

Volume 46 Issue 8

Apr. 2025

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2025 > 46(8): 371-381. > DOI: 10.13386/j.issn1002-0306.2024060299

CAO Weidong, LIU Lihong. Effects of Saponins of Semen Ziziphi Spinosae on Oxidative Damage of Liver and Kidney and Intestinal Microflora in Lead-exposed Mice[J]. Science and Technology of Food Industry, 2025, 46(8): 371−381. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060299.

Citation:

PDF (2223 KB)

Effects of Saponins of Semen Ziziphi Spinosae on Oxidative Damage of Liver and Kidney and Intestinal Microflora in Lead-exposed Mice

Changchun Vocational Institute of Technology, Changchun 130033, China

More Information

Received Date: June 20, 2024
Available Online: February 17, 2025

Graphical Abstract

Abstract

Abstract

Objective: To investigate the effects of saponins of Semen Ziziphi Spinosae (SSZS) on liver and kidney oxidative damage and intestinal flora composition in lead-exposed mice and explore its detoxification mechanism. Methods: The Pb-intoxicated mice model was induced by drinking water containing 0.05% lead acetate. Different concentrations of SSZS and DMSA were administered orally to the mice for treatment. During the experiment, the weight changes of the mice were recorded, and the Pb content of blood, liver, kidney and brain, TC and TG levels of plasma and liver, as well as oxidative stress related indicators in liver and kidney were measured. In addition, the expression of autophagy related genes in the liver and kidney was detected, and 16S rDNA sequencing technology was used to analyze changes in fecal intestinal flora of mice. Results: Compared with the Pb group, SSZS significantly reduced Pb content of the blood, liver, kidney and brain in mice, increased antioxidant enzyme activity in the liver and kidney, and improved lipid metabolism disorders caused by Pb exposure. In addition, SSZS inhibited that Pb induced excessive autophagy in liver and kidney cells by regulating the expression levels of autophagy related genes of Beclin1, LC3-Ⅱ, and Lamp2 mRNA of liver and kidney in mice, thereby reducing liver and kidney damage caused by Pb exposure. Moreover, SSES increased the richness and diversity of the fecal intestinal flora in mice, and increased the relative abundance of norank-f-Muribacaceae and Lactobacillus, decreased the relative abundance of Allobacterium, Staphylococcus, and Coriobacteriaceae-UCG-002, regulated the composition of intestinal flora in Pb-exposed mice, and improved gut health. Conclusion: SSZS can alleviate Pb poisoning in mice by regulating the composition of intestinal flora and the expression of autophagy genes in liver and kidney, increasing the antioxidant enzymes activity of liver and kidney, and improving lipid metabolism disorders caused by Pb exposure. The results of this study provides the theoretical foundation for SSZS as a natural anti-Pb agent.
- saponins of Semen Ziziphi Spinosae,
- Pb exposure,
- oxidative stress,
- intestinal flora

FullText(HTML)

References (28)

References

[1]	乔增运, 李昌泽, 周正, 等. 铅毒性危害及其治疗药物应用的研究进展[J]. 毒理学杂志,2020,34(5):416−420. [QIAO Z Y, LI C Z, ZHOU Z, et al. Research progress on lead toxicity hazards and their therapeutic drug applications[J]. Journal of Toxicology,2020,34(5):416−420.] QIAO Z Y, LI C Z, ZHOU Z, et al. Research progress on lead toxicity hazards and their therapeutic drug applications[J]. Journal of Toxicology, 2020, 34（5）: 416−420.
[2]	ALHUSAINI A M, FADDA L M, HASAN I H, et al. Role of some natural anti-oxidants in the down regulation of Kim, VCAM1, Cystatin C protein expression in lead acetateinduced acute kidney injury[J]. Pharmacology Reports,2020,72(2):360−367. doi: 10.1007/s43440-020-00072-8
[3]	LIU G, WANG Z K, WANG Z Y, et al. Mitochondrial permeability transition and its regulatory components are implicated in apoptosis of primary cultures of rat proximal tubular cells exposed to lead[J]. Archives of Toxicology,2016,90(5):1193−1209. doi: 10.1007/s00204-015-1547-0
[4]	李雨露, 李斌. 铅暴露对不同性别儿童神经系统影响差异的研究进展[J]. 毒理学杂志,2022,36(6):533−537,542. [LI Y L, LI B. Research progress on the differential effects of lead exposure on the nervous system of children of different genders[J]. Journal of Toxicology,2022,36(6):533−537,542.] LI Y L, LI B. Research progress on the differential effects of lead exposure on the nervous system of children of different genders[J]. Journal of Toxicology, 2022, 36（6）: 533−537,542.
[5]	BRETON J, DANIEL C, DEWULF J, et al. Gut microbiota limits heavy metals burden caused by chronic oral exposure[J]. Toxicology Letters,2013,222(2):132−138. doi: 10.1016/j.toxlet.2013.07.021
[6]	唐雪燕, 秦令祥, 曹源, 等. 酸枣仁总皂苷提取工艺优化及其改善睡眠作用机制研究[J]. 食品安全质量检测学报,2023,14(17):147−155. [TANG X Y, QIN L X, CAO Y, et al. Optimization of the extraction process of total saponins from jujube kernels and its mechanism of improving sleep[J]. Journal of Food Safety and Quality Testing,2023,14(17):147−155.] TANG X Y, QIN L X, CAO Y, et al. Optimization of the extraction process of total saponins from jujube kernels and its mechanism of improving sleep[J]. Journal of Food Safety and Quality Testing, 2023, 14（17）: 147−155.
[7]	LEE S Y, JU S K, LEE J H, et al. A new saponin from the seeds of Zizyphus jujuba var. spinosa[J]. Bulletin of The Korean Chemical Society,2013,34(2):657−660. doi: 10.5012/bkcs.2013.34.2.657
[8]	谢红. 酸枣仁皂苷的分离纯化及生物活性研究[D]. 广州:华南理工大学, 2021. [XIE H. Separation, purification, and biological activity study of jujube seed saponin[D]. Guangzhou:South China University of Technology, 2021.] XIE H. Separation, purification, and biological activity study of jujube seed saponin[D]. Guangzhou: South China University of Technology, 2021.
[9]	徐文祥, 李毅翔, 卞宏生, 等. Box-Behnken法优化酸枣仁总皂苷和多糖提取工艺及抗氧化作用研究[J]. 包装与食品机械,2022,40(6):20−24. [XU W X, LI Y X, BIAN H S, et al. Optimization of total saponins and polysaccharides extraction process and antioxidant activity of jujube seed by box behnken wethod[J]. Packaging and Food Machinery,2022,40(6):20−24.] doi: 10.3969/j.issn.1005-1295.2022.06.004 XU W X, LI Y X, BIAN H S, et al. Optimization of total saponins and polysaccharides extraction process and antioxidant activity of jujube seed by box behnken wethod[J]. Packaging and Food Machinery, 2022, 40（6）: 20−24. doi: 10.3969/j.issn.1005-1295.2022.06.004
[10]	赵盼, 李鸿梅, 王志超, 等. 罗耳阿太菌多糖经Nrf2通路预防小鼠肝脏铅损伤机制[J]. 食品工业科技,2022,43(20):395−402. [ZHAO P, LI H M, WANG Z C, et al. Mechanism of athelia rolfsii polysaccharides protect against liverInjury in lead-exposed mice via Nrf2 signaling pathway[J]. Science and Technology of Food Industry,2022,43(20):395−402.] ZHAO P, LI H M, WANG Z C, et al. Mechanism of athelia rolfsii polysaccharides protect against liverInjury in lead-exposed mice via Nrf2 signaling pathway[J]. Science and Technology of Food Industry, 2022, 43（20）: 395−402.
[11]	LIU Y Y, LI H L, REN P, et al. Polysaccharide from Flammulina velutipes residues protects mice from Pb poisoning by activating Akt/GSK3β/Nrf-2/HO-1 signaling pathway and modulating gut microbiota[J]. International Journal of Biological Macromolecules,2023,230:123154. doi: 10.1016/j.ijbiomac.2023.123154
[12]	董丽君. 褪黑素、维生素C对慢性铅中毒小鼠肝损伤的保护作用[D]. 重庆:西南大学, 2023. [DONG L J. Protective effects of melatonin and vitamin Con liver injury in mice with chronic lead poisoning[D]. Chongqing:Southwest University, 2023.] DONG L J. Protective effects of melatonin and vitamin Con liver injury in mice with chronic lead poisoning[D]. Chongqing: Southwest University, 2023.
[13]	KIPPLER M, LÖNNERDAL B, GOESSLER W, et al. Cadmium interacts with the transport of essential micronutrients in the mammary gland-a study in rural Bangladeshi women[J]. Toxicology,2008,257(12):64−69.
[14]	CHEN Z H, TANG Z Y, KONG J J, et al. Lactobacillus casei SYF-08 protects against Pb-induced injury in young mice by regulating bile acid metabolism and increasing Pb excretion[J]. Frontiers in Nutrition,2022,28(9):914323.
[15]	FUJIMORI T, TOYOMAKI H, SHIOTA K, et al. Lead speciation in body tissues, gastrointestinal contents, and feces of lead-exposed wild rats (Rattus rattus)[J]. Science of the Total Environment,2024,908:168297. doi: 10.1016/j.scitotenv.2023.168297
[16]	胡明月, 李鑫, 高磊, 等. 蒙古黄芪皂苷对铅暴露致发育期大鼠神经炎症与肠道菌群紊乱的影响[J]. 天津医药,2023,51(9):955−960. [HU M Y, LI X, GAO L, et al. The effect of Mongolian Astragalus saponins on lead exposure induced neuroinflammation and gut microbiota disorder in developing rats[J]. Tianjin Medical Journal,2023,51(9):955−960.] doi: 10.11958/20221821 HU M Y, LI X, GAO L, et al. The effect of Mongolian Astragalus saponins on lead exposure induced neuroinflammation and gut microbiota disorder in developing rats[J]. Tianjin Medical Journal, 2023, 51（9）: 955−960. doi: 10.11958/20221821
[17]	LI H, XIA X Y, CHENG S Z, et al. Oyster (Crassostrea gigas) ferritin relieves lead-induced liver oxidative damage via regulating the mitophagy[J]. International Journal of Biological Macromolecules,2023,253:126965. doi: 10.1016/j.ijbiomac.2023.126965
[18]	孙洪林. 铅暴露导致大鼠脂代谢紊乱的机制研究[D]. 上海:上海交通大学, 2017. [SUN H L. Mechanism of lipid metabolism disorder in rats induced by lead exposure[D]. Shanghai:Shanghai Jiao Tong University, 2017.] SUN H L. Mechanism of lipid metabolism disorder in rats induced by lead exposure[D]. Shanghai: Shanghai Jiao Tong University, 2017.
[19]	MATOVI V, BUH A, ÐUKI D, et al. Insight into the oxidative stress induced by lead and/or cadmium in blood, liver and kidneys[J]. Food and Chemical Toxicology,2015,78:130−140. doi: 10.1016/j.fct.2015.02.011
[20]	谢志明, 吴春梅, 陈少元, 等. 柴胡多糖通过抑制氧化应激和炎症反应减轻铅诱导小鼠肝肾损伤的研究[J]. 畜牧兽医学报,2021,52(9):2660−2672. [XIE Z M, WU C M, CHEN S Y, et al. Study on Chaihu polysaccharides reducing lead induced liver and kidney damage in mice by inhibiting oxidative stress and inflammatory response[J]. Journal of Animal Husbandry and Veterinary Medicine,2021,52(9):2660−2672.] doi: 10.11843/j.issn.0366-6964.2021.09.030 XIE Z M, WU C M, CHEN S Y, et al. Study on Chaihu polysaccharides reducing lead induced liver and kidney damage in mice by inhibiting oxidative stress and inflammatory response[J]. Journal of Animal Husbandry and Veterinary Medicine, 2021, 52（9）: 2660−2672. doi: 10.11843/j.issn.0366-6964.2021.09.030
[21]	KERR R P, KRUNKOSKY T M, HURLEY D J, et al. Lead at 2.5 and 5.0 μM induced aberrant MH-II surface expression through increased MII exocytosis and increased autophagosome formation in raw 267.4 cells[J]. Toxicology in Vitro,2013,27(3):1018−1024. doi: 10.1016/j.tiv.2013.01.018
[22]	ZHANG J B, CAO T J, ZHANG F, et al. The role of α-synuclein and tau hyperphosphorylation mediated autophagy and apoptosis in lead-induced learning and memory injury[J]. International Journal of Biological Sciences,2012,8(7):935−944. doi: 10.7150/ijbs.4499
[23]	LEVINE B, KROEMER G. Autophagy in the patho-genesis of disease[J]. Cell,2008,132(1):27−42. doi: 10.1016/j.cell.2007.12.018
[24]	田晓琳, 杨玲玲, 赵倩, 等. 氟与砷单独及联合暴露对子代大鼠肾脏毒性损伤与细胞自噬的影响[J]. 癌变·畸变·突变,2022,34(3):169−177. [TIAN X L, YANG L L, ZHAO Q, et al. The effects of fluoride and arsenic exposure alone and in combination on renal toxicity and cellular autophagy in offspring rats[J]. Cancer, Distortion, and Mutation,2022,34(3):169−177.] TIAN X L, YANG L L, ZHAO Q, et al. The effects of fluoride and arsenic exposure alone and in combination on renal toxicity and cellular autophagy in offspring rats[J]. Cancer, Distortion, and Mutation, 2022, 34（3）: 169−177.
[25]	卢星如, 吴蕊, 艾洪湖, 等. 绣球菌多糖对铅诱导小鼠肾脏氧化应激和自噬损伤的影响[J]. 动物营养学报,2023,35(12):8097−8110. [LU X R, WU R, AI H H, et al. The effect of polysaccharides from Hydrangea on lead induced oxidative stress and autophagic damage in the kidneys of mice[J]. Journal of Animal Nutrition,2023,35(12):8097−8110.] doi: 10.12418/CJAN2023.733 LU X R, WU R, AI H H, et al. The effect of polysaccharides from Hydrangea on lead induced oxidative stress and autophagic damage in the kidneys of mice[J]. Journal of Animal Nutrition, 2023, 35（12）: 8097−8110. doi: 10.12418/CJAN2023.733
[26]	BA Q, LI M, CHEN P Z, et al. Sex-dependent effects of cadmium exposure in early life on gut microbiota and fat accumulation in mice[J]. Environ Health Perspect,2017,125:437−446. doi: 10.1289/EHP360
[27]	屈定武, 翟齐啸, 于雷雷, 等. 多形拟杆菌对小鼠急性铅毒性的缓解作用[J]. 食品与发酵工业,2019,45(16):54−62. [QU D W, ZHAI Q X, YU L L, et al. Alleviative effect of Bacteroides multiforme on acute lead toxicity in mice[J]. Food and Fermentation Industry,2019,45(16):54−62.] QU D W, ZHAI Q X, YU L L, et al. Alleviative effect of Bacteroides multiforme on acute lead toxicity in mice[J]. Food and Fermentation Industry, 2019, 45（16）: 54−62.
[28]	YOON C H, RYU J S, MOON J, et al. Association between aging-dependent gut microbiome dysbiosis and dry eye severity in C57BL/6 male mouse model:A pilot study[J]. BMC Microbiology,2021,21:106. doi: 10.1186/s12866-021-02173-7

Supplements (0)

Cited By

Cited by

Periodical cited type(12)

1.	王琳，王耀，胡骁飞，王成宾，吴佳蓓. 免疫分析技术在金刚烷胺残留检测中的应用. 食品与机械. 2023(04): 205-210 .
2.	孙玲玲，尹营，耿旭浩，霍思宇，高家政，陈冬东，彭涛，薛占永. 动物源性食品中金刚烷胺类药物残留检测技术研究进展. 食品科技. 2023(08): 294-301 .
3.	任宏彬，张鑫鑫，贾晓婷，杨晓伟. 高效液相色谱-质谱联用法同时测定鸡肉中2种金刚烷胺类药物和12种喹诺酮类药物残留. 中国家禽. 2022(01): 77-82 .
4.	张艳，王全胜，吴银良. 分散固相萃取-液相色谱-串联质谱法同时测定鸡蛋中7种抗病毒类药物和利巴韦林代谢物残留量. 食品安全质量检测学报. 2022(06): 1872-1879 .
5.	郭东光，陈明艳，冯春花，王丰，卞爽丽，李文明，王凯露，吕薇薇，郑文珺，陈子怡，孙国鹏，李雪华，田金河，李鹏，朱艳平，岳锋，王选年. 齐帕特罗人工抗原合成及其多克隆抗体制备. 动物医学进展. 2022(05): 6-12 .
6.	林瑶. 畜禽肉食品中金刚烷胺金刚乙胺残留量的超高效液相色谱串联质谱法测定. 预防医学论坛. 2022(06): 438-441 .
7.	赵军强，韩典峰，田秀慧，王景，刘鸽，丁玉竹，彭中校，薛敬林，刘永春，乔瑞光，徐英江. 食品中金刚烷胺的危害、检测方法和残留风险研究进展. 中国渔业质量与标准. 2022(03): 64-71 .
8.	姜英杰，葛子欣，翟玮玮，苏晶. EMR-Lipid固相萃取结合液相色谱串联质谱法快速测定鸡蛋中金刚烷胺和金刚乙胺残留量. 中国食品添加剂. 2022(11): 218-224 .
9.	徐少华，聂婵，谭磊. 内标法测定鸡肉中金刚烷胺含量方法的优化——高效液相色谱-串联质谱法. 养禽与禽病防治. 2022(07): 20-23 .
10.	王丰存，端礼钦，王静，郑云天. 超声提取-固相萃取-超高效液相色谱串联质谱法同时测定畜禽产品中金刚烷胺及8种氟喹诺酮类药物残留. 中国检验检测. 2022(06): 52-55+79 .
11.	曾海英，王家磊，刘志敏，任召珍. 肉鸡中金刚烷胺残留测定及其残留代谢规律研究. 食品安全质量检测学报. 2021(08): 3118-3122 .
12.	彭娟，赖科洋. 食品中金刚烷胺残留检测方法新进展. 食品科学. 2021(19): 325-333 .