Mechanism of the Regulatory Effect of <i>Pediococcus pentosaceus</i> CQFP202437 on Antibiotic-induced Locomotor Dysfunction in Mice

LI Ke; YI Ruokun

doi:10.13386/j.issn1002-0306.2024060291

Volume 46 Issue 10

May 2025

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2025 > 46(10): 383-390. > DOI: 10.13386/j.issn1002-0306.2024060291

LI Ke, YI Ruokun. Mechanism of the Regulatory Effect of Pediococcus pentosaceus CQFP202437 on Antibiotic-induced Locomotor Dysfunction in Mice[J]. Science and Technology of Food Industry, 2025, 46(10): 383−390. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060291.

Citation:

PDF (3198 KB)

Mechanism of the Regulatory Effect of Pediococcus pentosaceus CQFP202437 on Antibiotic-induced Locomotor Dysfunction in Mice

LI Ke,
YI Ruokun^,

College of Physical Education and Health Management, Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing University of Education, Chongqing 400065

More Information

Received Date: June 20, 2024
Available Online: March 13, 2025

Graphical Abstract

Abstract

Abstract

To investigate the protective effect of Pediococcus pentosaceus CQFP202437 against antibiotic-induced locomotor disorders in mice, and to explore the effect and mechanism of probiotics, a mouse model of dyskinesia was constructed by intraperitoneal injection of sterile mixed antibiotic solution. After the modeling period, changes in exercise parameters such as running and swimming were analyzed in each group of mice, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), interleukin (IL-6, IL-10), and tumor necrosis factor (TNF-α) were measured in the serum and cerebrum of mice. The mRNA expression of cecum intestinal barrier genes Occludin-1, ZO-1 and Claudin-1, and the mRNA expression of CREB, ERK1/2 and BDNF genes in mouse brain were determined. Results indicated that Pediococcus pentosaceus CQFP202437 significantly increased both swimming and running duration in mice compared to the model group (P<0.01). Additionally, this treatment significantly (P<0.05) reduced the levels of inflammatory factors IL-6 and TNF-α in the mouse brain, increased the expression of SOD in the mouse brain, and reduced the accumulation of MDA in the mouse brain and serum. Furthermore, Pediococcus pentosaceus CQFP202437 improved the expression of BDNF, ERK1/2, CREB, and genes associated with the BDNF metabolic pathway in the cerebrum. It also upregulated the expression of the Occludin-1 gene in the cecum, which was crucial for maintaining intestinal barrier integrity and ensuring normal physiological function. These results suggested that a modulating effect on antibiotic-induced locomotor dysfunction in mice was exerted by Pediococcus pentosaceus CQFP202437, providing a theoretical foundation for the development of probiotic formulations aimed at enhancing locomotor function.
- Pediococcus pentosaceus,
- treadmill exercise,
- weighted swimming,
- brain derived neurotrophic factor (BDNF)

FullText(HTML)

References (31)

References

[1]	陈雨露. 运动联合益生菌补充对大鼠脂代谢和运动机能的影响[D]. 北京:北京体育大学, 2021: 42−50. [CHEN Yulu. Effects of exercise combined with probiotic supplementation on lipid metabolism and locomotor function in rats[D]. Beijing:Beijing Sport University, 2021: 42−50.] CHEN Yulu. Effects of exercise combined with probiotic supplementation on lipid metabolism and locomotor function in rats[D]. Beijing: Beijing Sport University, 2021: 42−50.
[2]	包大鹏. 运动性疲劳脑功能变化的fMRI研究[D]. 北京:北京体育大学, 2012:32−38. [BAO Dapeng. An fMRI study of functional brain changes in exercise fatigue[D]. Beijing:Beijing Sport University, 2012:32−38.] BAO Dapeng. An fMRI study of functional brain changes in exercise fatigue[D]. Beijing: Beijing Sport University, 2012: 32−38.
[3]	CANTORE R, PETROU I, LAVENDER S S, et al. In situ clinical effects of new dentifrices containing 1.5% arginine and fluoride on enamel de- and remineralization and plaque metabolism[J]. The Journal of clinical dentistry,2013,24:32−44.
[4]	SCHURR A. How the 'aerobic/anaerobic glycolysis' meme formed a 'habit of mind' which impedes progress in the field of brain energy metabolism[J]. International Journal of Molecular Sciences,2024,25(3):1433. doi: 10.3390/ijms25031433
[5]	叶梅聆, 孔梅, 张翔. 运动和氧化应激及机体的抗氧化系统[J]. 当代体育科技, 2016, 6(5):46,48. [YE Meiling, KONG Mei, ZHANG Xiang, Exercise and oxidative stress and the body's antioxidant system[J]. Contemporary Sports Science and Technology, 2016, 6(5):46,48.] YE Meiling, KONG Mei, ZHANG Xiang, Exercise and oxidative stress and the body's antioxidant system[J]. Contemporary Sports Science and Technology, 2016, 6（5）: 46,48.
[6]	曹奕炜, 宋睿, 吴宁, 等. 脑神经炎症对小鼠运动疲劳的影响及其可能机制[J]. 中国药理学与毒理学杂志,2023,37(9):655−663. [CAO Yiwei, SONG Rui, WU Ning, et al. Effect of neuroinflammation on exercise fatigue in mice and possible mechanism[J]. Chinese Journal of Pharmacology and Toxicology,2023,37(9):655−663.] doi: 10.3867/j.issn.1000-3002.2023.09.002 CAO Yiwei, SONG Rui, WU Ning, et al. Effect of neuroinflammation on exercise fatigue in mice and possible mechanism[J]. Chinese Journal of Pharmacology and Toxicology, 2023, 37（9）: 655−663. doi: 10.3867/j.issn.1000-3002.2023.09.002
[7]	STECKLING F M, LIMA F D, FARINHA J B, et al. Diclofenac attenuates inflammation through TLR4 pathway and improves exercise performance after exhaustive swimming[J]. Scandinavian Journal of Medicine and Science in Sports,2020,30(2):264−271. doi: 10.1111/sms.13579
[8]	DEMBITSKAYA Y, PIETTE C, PEREZ S, et al. Lactate supply overtakes glucose when neural computational and cognitive loads scale up[J]. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(47):e2212004119.
[9]	CASES J, ROMAIN C, MARÍN-PAGÁN C, et al. Supplementation with a polyphenol-rich extract, PerfLoad®, improves physical performance during high-intensity exercise:A randomized, double blind, crossover trial[J]. Nutrients,2017,9(4):421. doi: 10.3390/nu9040421
[10]	TANG Tian, WANG Jing, JIANG Yuanyuan, et al. Bifidobacterium lactis TY-S01 prevents loperamide-induced constipation by modulating gut microbiota and its metabolites in mice[J]. Frontiers in Nutrition,2022,9:890314. doi: 10.3389/fnut.2022.890314
[11]	YEH W L, HSU Y J, HO C S, et al. Lactobacillus plantarum Pl-02 supplementation combined with resistance training improved muscle mass, force, and exercise performance in mice[J]. Frontiers in Nutrition,2022,9:896503. doi: 10.3389/fnut.2022.896503
[12]	SUN Z H, HARRIS H M, MCCANN A, et al. Expanding the biotechnology potential of Lactobacilli through comparative genomics of 213 strains and associated genera[J]. Nature Communications,2015,6(1):8322−8334. doi: 10.1038/ncomms9322
[13]	CASTRO M, ASSMANN C, STEFANELLO N, et al. Caffeic acid attenuates neuroinflammation and cognitive impairment in streptozotocin-induced diabetic rats:Pivotal role of the cholinergic and purinergic signaling pathways[J]. Journal of Nutrition and Biochemistry, 2023, 115:109280.
[14]	DESBONNET L, CLARKE G, TRAPLIN A, et al. Gut microbiota depletion from early adolescence in mice:Implications for brain and behaviour[J]. Brain Behavior Immunity, 2015, 48:165−173.
[15]	岑燕霞, 梁玉才, 曾江赢, 等. 食源复方黄精组合物水提液对小鼠抗疲劳作用的研究[J], 2025, 46(2): 343−348. [CEN Yanxia, LIANG Yucai, ZENG Jiangying, et al. Anti-fatigue effect of water extract offood as medicine compoundpolygonati rhizoma composition on mice[J], 2025, 46(2): 343−348.] CEN Yanxia, LIANG Yucai, ZENG Jiangying, et al. Anti-fatigue effect of water extract offood as medicine compoundpolygonati rhizoma composition on mice[J], 2025, 46(2): 343−348.
[16]	SIES H. Oxidative stress:a concept in redox biology and medicine[J]. Redox Biology, 2015, 4:180−183.
[17]	SIES H. Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress:Oxidative eustress[J]. Redox Biology,2017,11:613−619. doi: 10.1016/j.redox.2016.12.035
[18]	VALKO M, LEIBFRITZ D, MONCOL J, et al. Free radicals and antioxidants in normal physiological functions and human disease[J]. International Journal of Biochemistry Cell Biology,2007,39(1):44−84. doi: 10.1016/j.biocel.2006.07.001
[19]	HE L, HE T, FARRAR S, et al. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species[J]. Cell Physiology Biochemistry,2017,44(2):532−553. doi: 10.1159/000485089
[20]	FORMAN H J, ZHANG H. Targeting oxidative stress in disease:promise and limitations of antioxidant therapy[J]. Nature Reviews. Drug Discovery,2021,20(9):689−709. doi: 10.1038/s41573-021-00233-1
[21]	NOGUEIRA C W, BARBOSA N V, ROCHA J. Toxicology and pharmacology of synthetic organoselenium compounds:An update[J]. Archives of Toxicology,2021,95(4):1179−1226. doi: 10.1007/s00204-021-03003-5
[22]	SHAH S J, BORLAUG B A, KITZMAN D W, et al. Research priorities for heart failure with preserved ejection fraction:national heart, lung, and blood institute working group summary[J]. Circulation,2020,141(12):1001−1026. doi: 10.1161/CIRCULATIONAHA.119.041886
[23]	HACKER G. Apoptosis in infection[J]. Microbes Infection,2018,20(9−10):552−559. doi: 10.1016/j.micinf.2017.10.006
[24]	ANDRESKA T, AUFMKOLK S, SAUER M, et al. High abundance of BDNF within glutamatergic presynapses of cultured hippocampal neurons[J]. Frontiers in Cellular Neuroscience,2014,8:107.
[25]	PARKHURST C N, YANG G, NINAN I, et al. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor[J]. Cell,2013,155(7):1596−1609. doi: 10.1016/j.cell.2013.11.030
[26]	MIURA S, KAI Y, TADAISHI M, et al. Marked phenotypic differences of endurance performance and exercise-induced oxygen consumption between AMPK and LKB1 deficiency in mouse skeletal muscle:changes occurring in the diaphragm[J]. American Journal of Physiology Endocrinology and Metabolism,2013,305(2):E213−E229. doi: 10.1152/ajpendo.00114.2013
[27]	BAZZONI G, MARTINEZ-ESTRADA O M, ORSENIGO F, et al. Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin[J]. The Journal of Biological Chemistry,2000,275(27):327−339.
[28]	ULLUWISHEWA D, ANDERSON R C, MCNABB W C, et al. Regulation of tight junction permeability by intestinal bacteria and dietary components[J]. The Journal of Nutrition,2011,141(5):148−159.
[29]	SHENG Kangliang, XU Yifan, KONG Xiaowei, et al. Probiotic Bacillus cereus alleviates dextran sulfate sodium-induced colitis in mice through improvement of the intestinal barrier function, anti-inflammation, and gut microbiota modulation[J]. Journal of Agricultural and Food Chemistry,2021,69:14810−14823. doi: 10.1021/acs.jafc.1c03375
[30]	KWEK E, YAN C, DING H, et al. Effects of hawthorn seed oil on plasma cholesterol and gut microbiota[J]. Nutrition Metabolism,2022,19:55−61. doi: 10.1186/s12986-022-00690-4
[31]	SHOME M, SONG L S, WILLIAMS S, et al. Serological profiling of Crohn’s disease and ulcerative colitis patients reveals anti-microbial antibody signatures[J]. World Journal of Gastroenterology,2022,28:4089−4101. doi: 10.3748/wjg.v28.i30.4089

[1]	LUO Tingting, LI Jingjing, HU Haiyue, HE Yajun, LI Yuzhuo, ZHAO Lingxuan, WANG Lina, YANG Chen, WANG Jianming. Optimization of Flaxseed Meal Protein Extraction Process and Study of Protein Structure and Functional Properties[J]. Science and Technology of Food Industry, 2025, 46(3): 258-268. DOI: 10.13386/j.issn1002-0306.2024030161
[2]	LU Yunfeng, DAI Taotao, LI Zhaoying, HAN Jialong, LI Ti, LIU Chengmei, CHEN Jun. Effects on the Functionality and Structure of Rice-Pea Composite Protein by Industry-scale Microfluidizer Combined with pH Cycling Treatment[J]. Science and Technology of Food Industry, 2025, 46(2): 83-93. DOI: 10.13386/j.issn1002-0306.2024020218
[3]	LI Yizhen, SONG Xinxin, MA Kaiyang, ZHANG Jian, WAN Xinyi, FENG Jin, CHEN Xiaoe, LI Ying, FANG Xubo. Effects of Modification on the Structure and Functional Properties of Dietary Fiber in Burdock Root[J]. Science and Technology of Food Industry, 2024, 45(18): 63-71. DOI: 10.13386/j.issn1002-0306.2023100115
[4]	Jinge WANG, Yongjian CAI, Junmei LIU, Qiangzhong ZHAO. Effect of Homogenization Assisted with Enzymatic Treatment on the Structural and Functional Properties of Soybean Protein Nanoparticles[J]. Science and Technology of Food Industry, 2023, 44(13): 85-93. DOI: 10.13386/j.issn1002-0306.2022090179
[5]	SHI Jiayi, ZHANG Tai, LIANG Fuqiang, SHI Yumeng. Changes of Structure and Functional Properties of Glutelin during Rice Storage[J]. Science and Technology of Food Industry, 2021, 42(6): 29-34,42. DOI: 10.13386/j.issn1002-0306.2020100061
[6]	WANG Yi, LIU Fan, ZANG Meng-lu, FANG Shi-wen, LI Xuan, XUE Feng. Effects of Ultrasound Treatment on the Functional Properties and Structure of Royal Jelly Proteins[J]. Science and Technology of Food Industry, 2019, 40(10): 50-56. DOI: 10.13386/j.issn1002-0306.2019.10.009
[7]	ZHOU Xiang-jun, ZHU Min-tao, YUAN Yi-jun. Effects of erythritol on structural and functional properties of pea protein isolate[J]. Science and Technology of Food Industry, 2018, 39(8): 73-77,84. DOI: 10.13386/j.issn1002-0306.2018.08.014
[8]	GUAN Yi-xian, HE Miao, XIONG Shuang-li. Effects of ultra high pressure treatment on functional properties and structure of rice proteins[J]. Science and Technology of Food Industry, 2016, (20): 104-109. DOI: 10.13386/j.issn1002-0306.2016.20.012
[9]	YANG Yong, BI Shuang, WANG Zhong-jiang, LI Yang, JIANG Lian-zhou. Effect of ultrasonic treatment on the structure and functional properties of mung bean protein[J]. Science and Technology of Food Industry, 2016, (09): 69-73. DOI: 10.13386/j.issn1002-0306.2016.09.005
[10]	TAN Hui, HAN Jian-chun, ZHANG Yuan, HE Pan, CUI Xian, LIU Rong-xu. Effect of high pressure homogenization on the function properties of soybean protein isolate-polysaccharide mixtures[J]. Science and Technology of Food Industry, 2015, (22): 92-96. DOI: 10.13386/j.issn1002-0306.2015.22.010