Selenization Optimization of Preparation Process of Polysaccharide from <i>Dendrobium huoshanense</i> and Its Inhibitory Effect on <i>α</i>-Amylase

CAO Wanxue; LI Jiao; TAO Qiang; FAN Xuanxuan; LU Jiting; CHEN Naifu; CHEN Naidong

doi:10.13386/j.issn1002-0306.2024060247

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CAO Wanxue, LI Jiao, TAO Qiang, et al. Selenization Optimization of Preparation Process of Polysaccharide from Dendrobium huoshanense and Its Inhibitory Effect on α-Amylase[J]. Science and Technology of Food Industry, 2025, 46(9): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060247.

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Selenization Optimization of Preparation Process of Polysaccharide from Dendrobium huoshanense and Its Inhibitory Effect on α-Amylase

CAO Wanxue^{1, 2, 3, 4,},
LI Jiao^{2, 3, 4},
TAO Qiang^{1, 2, 3, 4},
FAN Xuanxuan^{1, 2, 3, 4},
LU Jiting^{1, 2, 3, 4},
CHEN Naifu^{1, 2, 3, 4, ,},
CHEN Naidong^{1, 2, 3, 4, ,}

1.
College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China
2.
Anhui Province Key Lab of Quality Evaluation and Improvement of Traditional Chinese Medicine, Lu'an 237012, China
3.
College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China
4.
Quality Evaluation and Quality Improvement of Chinese Medicine Lu'an Laboratory, Lu'an 237012, China

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Received Date: June 16, 2024
Available Online: March 05, 2025

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Abstract

Objectives: This study aimed to optimize the selenization process of polysaccharides derived from Dendrobium huoshanense (DHP) and to investigate the inhibitory effects and mechanisms of both DHP and selenium-enriched DHP (Se-DHP) on α-amylase activity. Methods: To investigate the impact of factors such as the mass ratio of sodium selenite to DHP, reaction time, nitric acid concentration, and reaction temperature on the selenium content in Se-DHP, a single factor experiment was conducted. Optimization of the Se-DHP preparation conditions was achieved using response surface methodology, with selenium content as the response variable. Additionally, the inhibitory effects of DHP and Se-DHP on α-amylase activity were compared, and their inhibition mechanisms were examined through fluorescence quenching experiments. Results: The optimal conditions for selenization were identified as follows: reaction temperature of 70 ℃, reaction time of 460 minutes, a mass ratio of sodium selenite to DHP of 1:1, and a nitric acid concentration of 0.5%. Under these conditions, the selenium content in Se-DHP was 9223.51 μg/g, closely matching the predicted value of 9280.59 μg/g. Compared to DHP, Se-DHP demonstrated a significantly enhanced inhibitory effect on α-amylase. Fluorescence spectroscopy revealed that both DHP and Se-DHP exhibited static quenching mode for α-amylase. Conclusions: The findings provide a theoretical framework for the preparation of Se-DHP with high-selenium content and offer a reference for the potential use of selenium polysaccharides from DHP as a new selenium supplement in medicinal and nutritional applications.
- selenization,
- Dendrobium huoshanense polysaccharide,
- response surface methodology,
- α-amylase

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