YIN Yaxin, LIU Qirui, WANG Zhicheng, et al. Food-grade High Internal Phase Pickering Emulsions: Food-grade Solid Particles, Stabilization Mechanisms and Application in Encapsulating Probiotics[J]. Science and Technology of Food Industry, 2025, 46(8): 411−419. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060018.
Citation: YIN Yaxin, LIU Qirui, WANG Zhicheng, et al. Food-grade High Internal Phase Pickering Emulsions: Food-grade Solid Particles, Stabilization Mechanisms and Application in Encapsulating Probiotics[J]. Science and Technology of Food Industry, 2025, 46(8): 411−419. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024060018.

Food-grade High Internal Phase Pickering Emulsions: Food-grade Solid Particles, Stabilization Mechanisms and Application in Encapsulating Probiotics

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  • Received Date: June 05, 2024
  • Available Online: February 11, 2025
  • Probiotics are live microorganisms that provide various health benefits, such as enhancing immune function and regulating gut microbiota. However, their high sensitivity to environmental conditions makes them prone to inactivation during production, storage, and use. The microstructure of high internal phase Pickering emulsions (HIPPEs) droplets can accommodate probiotics in the oil phase, protecting them from contact with water and thereby improving their survival rate. Here, the various types of food-grade solid particles used to stabilize Pickering emulsions and their mechanisms are firstly reviewed in this paper, including wetting properties at the interface, steric hindrance mechanisms, and three-dimensional network mechanisms. Furthermore, the application of HIPPEs in probiotic encapsulation is elaborated, focusing on the characteristics and differences of various types of HIPPEs in terms of probiotic encapsulation and delivery. Finally, the development prospects and future research directions of HIPPEs are discussed, aiming to provide a theoretical basis and new ideas for the development of probiotic encapsulation technologies.
  • [1]
    DIANAWATI D, MISHRA V, SHAH N P. Survival of microencapsulated probiotic bacteria after processing and during storage:A review[J]. Critical Reviews in Food Science and Nutrition,2015,56(10):1685−1716.
    [2]
    JIANG Z W, LI M T, MCCLEMENTS D J, et al. Recent advances in the design and fabrication of probiotic delivery systems to target intestinal inflammation[J]. Food Hydrocolloids,2022,125:107438. doi: 10.1016/j.foodhyd.2021.107438
    [3]
    XIAO Y, LU C B, LIU Y Y, et al. Encapsulation of Lactobacillus rhamnosus in hyaluronic acid-based hydrogel for pathogen-targeted delivery to ameliorate enteritis[J]. ACS Appl Mater Interfaces,2020,12(33):36967−36977. doi: 10.1021/acsami.0c11959
    [4]
    GAO Y X, WANG X, XUE C H, et al. Latest developments in food-grade delivery systems for probiotics:A systematic review[J]. Critical Reviews in Food Science and Nutrition,2021,63(20):4371−4388.
    [5]
    GONZALEZ ORTIZ D, POCHAT-BOHATIER C, CAMBEDOUZOU J, et al. Current trends in Pickering emulsions:Particle morphology and applications[J]. Engineering,2020,6(4):468−482. doi: 10.1016/j.eng.2019.08.017
    [6]
    LIU X, XIE F, ZHOU J J, et al. High internal phase Pickering emulsion stabilized by zein-tannic acid-sodium alginate complexes:β-Carotene loading and 3D printing[J]. Food Hydrocolloids,2023,142:108762. doi: 10.1016/j.foodhyd.2023.108762
    [7]
    JIAO B, SHI A M, WANG Q, et al. High-internal-phase Pickering emulsions stabilized solely by peanut protein microgel particles with multiple potential applications[J]. Angewandte Chemie International Edition,2018,57(30):9274−9278. doi: 10.1002/anie.201801350
    [8]
    ZHOU F Z, YU X H, ZENG T, et al. Fabrication and characterization of novel water-insoluble protein porous materials derived from Pickering high internal-phase emulsions stabilized by gliadin–chitosan-complex particles[J]. Journal of Agricultural and Food Chemistry,2019,67(12):3423−3431. doi: 10.1021/acs.jafc.9b00221
    [9]
    刘树萍, 彭秀文, 张佳美, 等. 大豆分离蛋白与茶多酚稳定的高内相Pickering乳液替代脂肪对肉丸品质的影响[J]. 食品工业科技,2024,45(6):59−66. [LIU S P, PENG X W, ZHANG J M, et al. Effect of soybean protein isolate and tea polyphenol stabilized high interior phase Pickering emulsion replacing fat on meatball quality[J]. Science and Technology of Food Industry,2024,45(6):59−66.]

    LIU S P, PENG X W, ZHANG J M, et al. Effect of soybean protein isolate and tea polyphenol stabilized high interior phase Pickering emulsion replacing fat on meatball quality[J]. Science and Technology of Food Industry, 2024, 45(6): 59−66.
    [10]
    SU J L, WANG X Q, LI W, et al. Enhancing the viability of Lactobacillus plantarum as probiotics through encapsulation with high internal phase emulsions stabilized with whey protein isolate microgels[J]. Journal of Agricultural and Food Chemistry,2018,66(46):12335−12343. doi: 10.1021/acs.jafc.8b03807
    [11]
    WU C, LIU Z, ZHI L Y, et al. Research progress of food-grade high internal phase Pickering emulsions and their application in 3D printing[J]. Nanomaterials,2022,12(17):2949. doi: 10.3390/nano12172949
    [12]
    BI C H, CHI S Y, ZHOU T, et al. Characterization of a novel high internal phase Pickering emulsions stabilized by soy protein self-assembled gel particles[J]. Frontiers in Nutrition,2021,8:795396. doi: 10.3389/fnut.2021.795396
    [13]
    WU J D, SHI M X, LI W, et al. Pickering emulsions stabilized by whey protein nanoparticles prepared by thermal cross-linking[J]. Colloids and Surfaces B:Biointerfaces,2015,127:96−104. doi: 10.1016/j.colsurfb.2015.01.029
    [14]
    ZHANG X Y, ZHANG S, ZHONG M M, et al. Soy and whey protein isolate mixture/calcium chloride thermally induced emulsion gels:Rheological properties and digestive characteristics[J]. Food Chemistry,2022,380:132212. doi: 10.1016/j.foodchem.2022.132212
    [15]
    FANG Y, DALGLEISH D G. Dimensions of the adsorbed layers in oil-in-water emulsions stabilized by caseins[J]. Journal of Colloid and Interface Science,1993,156(2):329−334. doi: 10.1006/jcis.1993.1120
    [16]
    GUO Y, WU C, DU M, et al. In-situ dispersion of casein to form nanoparticles for Pickering high internal phase emulsions[J]. LWT,2021,139:110538. doi: 10.1016/j.lwt.2020.110538
    [17]
    WIJAYA W, VAN DER MEEREN P, WIJAYA C H, et al. High internal phase emulsions stabilized solely by whey protein isolate-low methoxyl pectin complexes:Effect of pH and polymer concentration[J]. Food & Function,2017,8(2):584−594.
    [18]
    LIU W, GAO H X, MCCLEMENTS D J, et al. Stability, rheology, and β-carotene bioaccessibility of high internal phase emulsion gels[J]. Food Hydrocolloids,2019,88:210−217. doi: 10.1016/j.foodhyd.2018.10.012
    [19]
    ZAMANI S, MALCHIONE N, SELIG M J, et al. Formation of shelf stable Pickering high internal phase emulsions (HIPE) through the inclusion of whey protein microgels[J]. Food & Function,2018,9(2):982−990.
    [20]
    TAN H, TU Z, JIA H Q, et al. Hierarchical porous protein scaffold templated from high internal phase emulsion costabilized by gelatin and gelatin nanoparticles[J]. Langmuir,2018,34(16):4820−4829. doi: 10.1021/acs.langmuir.7b04047
    [21]
    王斌. 二硫键在疏水蛋白自组装过程中的功能研究[D]. 天津:天津大学, 2019. [WANG B. Functional study of the disulfide bridges in the self-assembly of hydrophobin[D]. Tianjin:Tianjin University, 2019.]

    WANG B. Functional study of the disulfide bridges in the self-assembly of hydrophobin[D]. Tianjin: Tianjin University, 2019.
    [22]
    孟新宇. 壳聚糖的疏水改性及其乳化性能研究[D]. 无锡:江南大学, 2022. [MENG X Y. Hydrophobic modification of chitosan and the emulsifying properties[D]. Wuxi:Jiangnan University, 2022.]

    MENG X Y. Hydrophobic modification of chitosan and the emulsifying properties[D]. Wuxi: Jiangnan University, 2022.
    [23]
    LIU F, TANG C H. Soy protein nanoparticle aggregates as Pickering stabilizers for oil-in-water emulsions[J]. Journal of Agricultural and Food Chemistry,2013,61(37):8888−8898. doi: 10.1021/jf401859y
    [24]
    XU Y T, LIU T X, TANG C H. Novel pickering high internal phase emulsion gels stabilized solely by soy β-conglycinin[J]. Food Hydrocolloids,2019,88:21−30. doi: 10.1016/j.foodhyd.2018.09.031
    [25]
    HU Y Q, YIN S W, ZHU J H, et al. Fabrication and characterization of novel Pickering emulsions and Pickering high internal emulsions stabilized by gliadin colloidal particles[J]. Food Hydrocolloids,2016,61:300−310. doi: 10.1016/j.foodhyd.2016.05.028
    [26]
    YANG T, ZHENG J, ZHENG B S, et al. High internal phase emulsions stabilized by starch nanocrystals[J]. Food Hydrocolloids,2018,82:230−238. doi: 10.1016/j.foodhyd.2018.04.006
    [27]
    QIAO M, YANG X C, ZHU Y, et al. Ultralight aerogels with hierarchical porous structures prepared from cellulose nanocrystal stabilized Pickering high internal phase emulsions[J]. Langmuir,2020,36(23):6421−6428. doi: 10.1021/acs.langmuir.0c00646
    [28]
    ZHU Y, HUAN S Q, BAI L, et al. High internal phase oil-in-water Pickering emulsions stabilized by chitin nanofibrils:3D structuring and solid foam [J]. ACS Applied Materials & Interfaces,2020,12(9):11240−11251.
    [29]
    佟臻, 韦阳, 高彦祥. 基于食品级胶体颗粒稳定Pickering乳液的研究进展[J]. 食品工业科技,2019,40(4):317−324. [TONG Z, WEI Y, GAO Y X. Research progress of stabilized Pickering emulsion based on food grade colloidal particles[J]. Science and Technology of Food Industry,2019,40(4):317−324.]

    TONG Z, WEI Y, GAO Y X. Research progress of stabilized Pickering emulsion based on food grade colloidal particles[J]. Science and Technology of Food Industry, 2019, 40(4): 317−324.
    [30]
    WANG C, PEI X P, TAN J L, et al. Thermoresponsive starch-based particle-stabilized Pickering high internal phase emulsions as nutraceutical containers for controlled release[J]. International Journal of Biological Macromolecules,2020,146:171−178. doi: 10.1016/j.ijbiomac.2019.12.269
    [31]
    CHEN Q H, ZHENG J, XU Y T, et al. Surface modification improves fabrication of Pickering high internal phase emulsions stabilized by cellulose nanocrystals[J]. Food Hydrocolloids,2018,75:125−130. doi: 10.1016/j.foodhyd.2017.09.005
    [32]
    PERRIN E, BIZOT H, CATHALA B, et al. Chitin nanocrystals for Pickering high internal phase emulsions[J]. Biomacromolecules,2014,15(10):3766−3771. doi: 10.1021/bm5010417
    [33]
    MUXIKA A, ETXABIDE A, URANGA J, et al. Chitosan as a bioactive polymer:Processing, properties and applications[J]. International Journal of Biological Macromolecules,2017,105:1358−1368. doi: 10.1016/j.ijbiomac.2017.07.087
    [34]
    SUN G G, ZHAO Q F, LIU S L, et al. Complex of raw chitin nanofibers and zein colloid particles as stabilizer for producing stable Pickering emulsions[J]. Food Hydrocolloids,2019,97:105178. doi: 10.1016/j.foodhyd.2019.105178
    [35]
    SHARKAWY A, BARREIRO M F, RODRIGUES A E. Chitosan-based Pickering emulsions and their applications:A review[J]. Carbohydrate Polymers,2020,250:116885. doi: 10.1016/j.carbpol.2020.116885
    [36]
    HUANG C, SUN F S, MA X X, et al. Hydrophobically modified chitosan microgels stabilize high internal phase emulsions with high compliance[J]. Carbohydrate Polymers,2022,288:119277. doi: 10.1016/j.carbpol.2022.119277
    [37]
    SONG Y, ZHOU L Y, ZHANG D C, et al. Stability and release of peach polyphenols encapsulated by Pickering high internal phase emulsions in vitro and in vivo[J]. Food Hydrocolloids,2023,139:108593. doi: 10.1016/j.foodhyd.2023.108593
    [38]
    MA L, ZOU L Q, MCCLEMENTS D J, et al. One-step preparation of high internal phase emulsions using natural edible Pickering stabilizers:Gliadin nanoparticles/gum Arabic[J]. Food Hydrocolloids,2020,100:105381. doi: 10.1016/j.foodhyd.2019.105381
    [39]
    ZENG T, WU Z L, ZHU J Y, et al. Development of antioxidant Pickering high internal phase emulsions (HIPEs) stabilized by protein/polysaccharide hybrid particles as potential alternative for PHOs[J]. Food Chemistry,2017,231:122−130. doi: 10.1016/j.foodchem.2017.03.116
    [40]
    SHEN R, LIN D H, LIU Z, et al. Fabrication of bacterial cellulose nanofibers/soy protein isolate colloidal particles for the stabilization of high internal phase Pickering emulsions by anti-solvent precipitation and their application in the delivery of curcumin[J]. Frontiers in Nutrition,2021,8:734620. doi: 10.3389/fnut.2021.734620
    [41]
    LIU X, HUANG Y Q, CHEN X W, et al. Whole cereal protein-based Pickering emulsions prepared by zein-gliadin complex particles[J]. Journal of Cereal Science,2019,87:46−51. doi: 10.1016/j.jcs.2019.02.004
    [42]
    WANG T, LI F S, ZHANG H, et al. Plant-based high internal phase emulsions stabilized by dual protein nanostructures with heat and freeze–thaw tolerance[J]. Food Chemistry,2022,373:131458. doi: 10.1016/j.foodchem.2021.131458
    [43]
    PANG B, LIU H, REHFELDT F, et al. High internal phase Pickering emulsions stabilized by dialdehyde amylopectin/chitosan complex nanoparticles[J]. Carbohydrate Polymers,2021,258:117655. doi: 10.1016/j.carbpol.2021.117655
    [44]
    YAN C, MCCLEMENTS D J, ZHU Y, et al. Fabrication of OSA starch/chitosan polysaccharide-based high internal phase emulsion via altering interfacial behaviors[J]. Journal of Agricultural and Food Chemistry,2019,67(39):10937−10946. doi: 10.1021/acs.jafc.9b04009
    [45]
    XIAO J, LI Y Q, HUANG Q R. Recent advances on food-grade particles stabilized Pickering emulsions:Fabrication, characterization and research trends[J]. Trends in Food Science & Technology,2016,55:48−60.
    [46]
    LI X M, ZHU J, PAN Y, et al. Fabrication and characterization of pickering emulsions stabilized by octenyl succinic anhydride -modified gliadin nanoparticle[J]. Food Hydrocolloids,2019,90:19−27. doi: 10.1016/j.foodhyd.2018.12.012
    [47]
    LI F F, LI X H, HUANG K L, et al. Preparation and characterization of pickering emulsion stabilized by hordein-chitosan complex particles[J]. Journal of Food Engineering,2021,292:110275. doi: 10.1016/j.jfoodeng.2020.110275
    [48]
    吴昱春, 陈小草, 张琦, 等. Pickering乳液稳定机理及其在食品中的应用研究进展[J]. 食品科学, 2021, 42(7):275−282. [WU Y C, CHEN X C, ZHANG Q, et al. Stability mechanism of Pickering emulsion and its application in food industry:A review[J] Food Science, 2021, 42(7):275−282.]

    WU Y C, CHEN X C, ZHANG Q, et al. Stability mechanism of Pickering emulsion and its application in food industry: A review[J] Food Science, 2021, 42(7): 275−282.
    [49]
    SUN F W, CHENG T F, REN S H, et al. Soy protein isolate/carboxymethyl cellulose sodium complexes system stabilized high internal phase Pickering emulsions:Stabilization mechanism based on noncovalent interaction[J]. International Journal of Biological Macromolecules,2024,256:128381. doi: 10.1016/j.ijbiomac.2023.128381
    [50]
    LIU F, TANG C H. Emulsifying Properties of soy protein nanoparticles:Influence of the protein concentration and/or emulsification process[J]. Journal of Agricultural and Food Chemistry,2014,62(12):2644−2654. doi: 10.1021/jf405348k
    [51]
    HOROZOV T S, BINKS B P. Particle-stabilized emulsions:A bilayer or a bridging monolayer?[J]. Angewandte Chemie,2006,118(5):787−790. doi: 10.1002/ange.200503131
    [52]
    WANG S Y, LIU L G, BI S H, et al. Studies on stabilized mechanism of high internal phase Pickering emulsions from the collaboration of low dose konjac glucomannan and myofibrillar protein[J]. Food Hydrocolloids,2023,143:108862. doi: 10.1016/j.foodhyd.2023.108862
    [53]
    XU Y T, TANG C H, BINKS B P. High internal phase emulsions stabilized solely by a globular protein glycated to form soft particles[J]. Food Hydrocolloids,2020,98:105254. doi: 10.1016/j.foodhyd.2019.105254
    [54]
    XU Y T, TANG C H, BINKS B P. Ultraefficient stabilization of high internal phase emulsions by globular proteins in the presence of polyols:Importance of a core-shell nanostructure[J]. Food Hydrocolloids,2020,107:105968. doi: 10.1016/j.foodhyd.2020.105968
    [55]
    IRAVANI S, KORBEKANDI H, MIRMOHAMMADI S V. Technology and potential applications of probiotic encapsulation in fermented milk products[J]. Journal of Food Science and Technology,2014,52(8):4679−4696.
    [56]
    HIGL B, KURTMANN L, CARLSEN C U, et al. Impact of water activity, temperature, and physical state on the storage stability of Lactobacillus paracasei ssp. paracasei freeze-dried in a lactose matrix[J]. Biotechnology Progress,2007,23(4):794−800. doi: 10.1002/bp070089d
    [57]
    陈臣, 张晓丛, 袁海彬, 等. 益生菌包埋前沿技术及其研究进展[J]. 中国食品学报,2023,23(1):384−396. [CHEN C, ZHANG X C, YUAN H B, et al. Research progress on the advanced technology of embedding for probiotics[J]. Journal of Chinese Institute of Food Science and Technology,2023,23(1):384−396.]

    CHEN C, ZHANG X C, YUAN H B, et al. Research progress on the advanced technology of embedding for probiotics[J]. Journal of Chinese Institute of Food Science and Technology, 2023, 23(1): 384−396.
    [58]
    ESLAMI P, DAVARPANAH L, VAHABZADEH F. Encapsulating role of β-cyclodextrin in formation of pickering water-in-oil-in-water (W1/O/W2) double emulsions containing Lactobacillus dellbrueckii[J]. Food Hydrocolloids,2017,64:133−148. doi: 10.1016/j.foodhyd.2016.10.035
    [59]
    GAO H X, MA L, CHENG C, et al. Review of recent advances in the preparation, properties, and applications of high internal phase emulsions[J]. Trends in Food Science & Technology,2021,112:36−49.
    [60]
    QIN X S, GAO Q Y, LUO Z G. Enhancing the storage and gastrointestinal passage viability of probiotic powder (Lactobacillus Plantarum) through encapsulation with pickering high internal phase emulsions stabilized with WPI-EGCG covalent conjugate nanoparticles[J]. Food Hydrocolloids,2021,116:106658. doi: 10.1016/j.foodhyd.2021.106658
    [61]
    SU J Q, CAI Y J, TAI K D, et al. High-internal-phase emulsions (HIPEs) for co-encapsulation of probiotics and curcumin:Enhanced survivability and controlled release[J]. Food & Function,2021,12(1):70−82.
    [62]
    GAO H X, MA L, SUN W X, et al. Impact of encapsulation of probiotics in oil-in-water high internal phase emulsions on their thermostability and gastrointestinal survival[J]. Food Hydrocolloids,2022,126:107478. doi: 10.1016/j.foodhyd.2021.107478
    [63]
    SUN C Y, WANG S N, HUANG X Y, et al. Enhancing probiotic viability:Impact of soy hull polysaccharide concentration on stabilized high-internal-phase emulsions encapsulated with Lactobacillus plantarum and their release during gastrointestinal digestive[J]. Food Hydrocolloids,2024,152:109959. doi: 10.1016/j.foodhyd.2024.109959
    [64]
    ZHANG Y, XIE Y F, LIU H, et al. Probiotic encapsulation in water-in-oil high internal phase emulsions:Enhancement of viability under food and gastrointestinal conditions[J]. LWT,2022,163:113499. doi: 10.1016/j.lwt.2022.113499
    [65]
    WANG J S, CHEN L M. Impact of a novel nano-protectant on the viability of probiotic bacterium Lactobacillus casei K17[J]. Foods,2021,10(3):529. doi: 10.3390/foods10030529
    [66]
    RATTANABURI P, CHAROENRAT N, PONGTHARANGKUL T, et al. Hydroxypropyl methylcellulose enhances the stability of o/w Pickering emulsions stabilized with chitosan and the whole cells of Lactococcus lactis IO-1[J]. Food Research International,2019,116:559−565. doi: 10.1016/j.foodres.2018.08.074
    [67]
    RUAN M J, XIE Y X, ZHOU C Y, et al. Biomacromolecule based water-in-water Pickering emulsion:A fascinating artificial cell-like compartment for the encapsulation of Lactobacillus plantarum[J]. Food Bioscience,2023,55:102916. doi: 10.1016/j.fbio.2023.102916
    [68]
    WANG L, SONG M Y, ZHAO Z J, et al. Lactobacillus acidophilus loaded Pickering double emulsion with enhanced viability and colon-adhesion efficiency[J]. Lwt,2020,121:108928. doi: 10.1016/j.lwt.2019.108928
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