<i>Hovenia dulcis</i> Fruit Peduncle Polysaccharides Modulate Glutamate Metabolism and Tight Junction Protein Expressions to Attenuate the Neurotoxic Effects of Alcohol on Mice

ZHANG Yuchao; YUAN Zifei; LIU Liangyu; ZHU Sijie; YANG Yong; ZHENG Daimei; LIU Xudong

doi:10.13386/j.issn1002-0306.2024050346

Volume 46 Issue 9

Apr. 2025

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Science and Technology of Food Industry > 2025 > 46(9): 372-380. > DOI: 10.13386/j.issn1002-0306.2024050346

ZHANG Yuchao, YUAN Zifei, LIU Liangyu, et al. Hovenia dulcis Fruit Peduncle Polysaccharides Modulate Glutamate Metabolism and Tight Junction Protein Expressions to Attenuate the Neurotoxic Effects of Alcohol on Mice[J]. Science and Technology of Food Industry, 2025, 46(9): 372−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050346.

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Hovenia dulcis Fruit Peduncle Polysaccharides Modulate Glutamate Metabolism and Tight Junction Protein Expressions to Attenuate the Neurotoxic Effects of Alcohol on Mice

1.
Department of Brewing Engineering, Moutai Institute, Renhuai 564507, China
2.
Department of Food Science and Engineering, Moutai Institute, Renhuai 564507, China
3.
College of Food Science and Engineering, Ocean University of China, Qingdao 266071, China

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Received Date: May 26, 2024
Available Online: March 05, 2025

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Abstract

Abstract

Objective: This study aimed to investigate the protective effects of Hovenia dulcis fruit peduncle polysaccharides (HDPs) on alcohol-induced neurobehavioral alterations in mice and to elucidate whether HDPs mitigate alcohol-induced neuronal damage by modulating glutamate metabolic pathway and the expression of tight junction protein. Methods: Male C57BL/6 mice were administered alcohol intragastrically at a dose of 114 μL/20 g for 14 d to establish an alcohol exposure model, and an intervention group was set up for HDPs intervention (114 μL/20 g alcohol+100 mg/kg HDPs). Behavioral experiments (open field test, elevated plus maze test) were used to assess changes in neurobehavior, gas chromatography was used to determine ethanol concentrations in mouse blood. γ-H2AX fluorescence was used to detect DNA damage in mouse hippocampal tissue, and immunohistochemical analysis was used to detect the expression of tight junction proteins Claudin-1 and ZO-1 in mouse brain tissue. Metabolites in mouse brain tissue were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) metabolomics technology. Results: HDPs effectively reduced blood ethanol concentration in alcohol-exposed mice (from 4.69±0.29 g/L to 1.64±0.104 g/L) and ameliorated alcohol-induced neurobehavioral abnormalities. In the open field test, compared to the alcohol group, HDPs intervention significantly increased total distance traveled (27340±3304 cm, P<0.05) and average velocity (67.4±13.4 cm/s, P<0.05), while reduced immobility time by 29% (P<0.05). The elevated plus maze test revealed that HDPs treatment decreased closed-arm dwelling time (195.6±10.3 s, P<0.05) and increased open-arm entries by 26% (P<0.05) compared to the alcohol group. Additionally, HDPs alleviated alcohol-induced oxidative brain damage by reducing ROS levels (5.4%) and MDA content (29.5%) (P<0.05) respectively, while enhanced total antioxidant capacity (T-AOC) by 10.9%. It upregulated hippocampal expression of tight junction proteins Claudin-1 (2.2-fold) and ZO-1 (10%). HDPs also modulated glutamatergic metabolic pathways, elevating brain levels of glycine, glutathione, and succinate by 19.7%, 25% and 22.6% respectively. Conclusion: HDPs can effectively alleviate the neurobehavioral impact of alcohol on mice. The mechanism may involve antioxidant activity, protection of tight junction proteins, and modulation of glutamate metabolic pathways, providing a theoretical basis for the development and application of Hovenia dulcisis resources in the food field.
- Hovenia dulcis fruit peduncle polysaccharides,
- alcohol,
- neurobehavior,
- glutamate metabolism,
- tight junction proteins

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