Effect of Pectin Oligosaccharides from Hawthorn on the H<sub>2</sub>O<sub>2</sub>-induced Human Embryonic Lung Fibroblast Cells

LI Tao; JI Wenhua; DONG Hongjing; WANG Zhenqiang; HUANG Qun; WANG Xiao

doi:10.13386/j.issn1002-0306.2024050308

Volume 46 Issue 7

Mar. 2025

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Science and Technology of Food Industry > 2025 > 46(7): 346-354. > DOI: 10.13386/j.issn1002-0306.2024050308

LI Tao, JI Wenhua, DONG Hongjing, et al. Effect of Pectin Oligosaccharides from Hawthorn on the H₂O₂-induced Human Embryonic Lung Fibroblast Cells[J]. Science and Technology of Food Industry, 2025, 46(7): 346−354. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050308.

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Effect of Pectin Oligosaccharides from Hawthorn on the H₂O₂-induced Human Embryonic Lung Fibroblast Cells

LI Tao^{1, 2,},
JI Wenhua^{1, 2},
DONG Hongjing^{1, 2},
WANG Zhenqiang³,
HUANG Qun^4, ,,
WANG Xiao^{1, 2, ,}

1.
Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
2.
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
3.
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
4.
Guizhou Ecological Food Creation Engineering Technology Center, Guizhou Medical University, Guian New Area 550025, China

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Received Date: May 26, 2024
Available Online: January 21, 2025

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Abstract

Abstract

In this study, the anti-aging effects and mechanism of action of pectin oligosaccharides from hawthorn (HPOS) on H₂O₂-induced human embryonic lung fibroblast cells were evaluated using the MRC-5 cell line. The effects on cell viability, survival rate, β-galactosidase activity, mitochondrial membrane potential, the content of reactive oxygen species (ROS), antioxidant capacity, and the expression levels of proteins associated with the p53/p21/p16 signaling pathway were evaluated. The findings illustrated that compared with the model group, under the intervention of HPOS at a concentration of 0.8 mg/mL, the cell viability, survival rate, and mitochondrial membrane potential of MRC-5 cells increased by 16.4%, 17.0%, and 284.3% (P<0.01) , respectively. Furthermore, the proportion of β-galactosidase-positive cells and fluorescence intensity of ROS significantly decreased by 66.9%, 77.2% (P<0.01), respectively. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) contents increased by 72.0% and 50.0%, respectively. Compared to that in the model group, the malondialdehyde (MDA) content decreased by 42.6% (P<0.01) significantly. HPOS at a concentration of 0.8 mg/mL significantly inhibited p53, p21, and p16 expression in the p53/p21/p16 pathway (P<0.01). Thus, HPOS ameliorates the H₂O₂-induced aging of MRC-5 cells by inhibiting apoptosis and oxidative stress damage along with regulating the p53/p21/p16 pathway, thereby providing a scientific basis for the development of HPOS as a functional food ingredient that acts against aging.
- pectin oligosaccharides from hawthorn,
- aging,
- cell apoptosis,
- antioxidant capacity,
- p53/p21/p16 pathway

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References

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