Effect of Pectin Oligosaccharides from Hawthorn on the H<sub>2</sub>O<sub>2</sub>-induced Human Embryonic Lung Fibroblast Cells

LI Tao; JI Wenhua; DONG Hongjing; WANG Zhenqiang; HUANG Qun; WANG Xiao

doi:10.13386/j.issn1002-0306.2024050308

Volume 46 Issue 7

Mar. 2025

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Science and Technology of Food Industry > 2025 > 46(7): 346-354. > DOI: 10.13386/j.issn1002-0306.2024050308

LI Tao, JI Wenhua, DONG Hongjing, et al. Effect of Pectin Oligosaccharides from Hawthorn on the H₂O₂-induced Human Embryonic Lung Fibroblast Cells[J]. Science and Technology of Food Industry, 2025, 46(7): 346−354. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050308.

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Effect of Pectin Oligosaccharides from Hawthorn on the H₂O₂-induced Human Embryonic Lung Fibroblast Cells

LI Tao^{1, 2,},
JI Wenhua^{1, 2},
DONG Hongjing^{1, 2},
WANG Zhenqiang³,
HUANG Qun^4, ,,
WANG Xiao^{1, 2, ,}

1.
Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
2.
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
3.
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
4.
Guizhou Ecological Food Creation Engineering Technology Center, Guizhou Medical University, Guian New Area 550025, China

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Received Date: May 26, 2024
Available Online: January 21, 2025

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Abstract

Abstract

In this study, the anti-aging effects and mechanism of action of pectin oligosaccharides from hawthorn (HPOS) on H₂O₂-induced human embryonic lung fibroblast cells were evaluated using the MRC-5 cell line. The effects on cell viability, survival rate, β-galactosidase activity, mitochondrial membrane potential, the content of reactive oxygen species (ROS), antioxidant capacity, and the expression levels of proteins associated with the p53/p21/p16 signaling pathway were evaluated. The findings illustrated that compared with the model group, under the intervention of HPOS at a concentration of 0.8 mg/mL, the cell viability, survival rate, and mitochondrial membrane potential of MRC-5 cells increased by 16.4%, 17.0%, and 284.3% (P<0.01) , respectively. Furthermore, the proportion of β-galactosidase-positive cells and fluorescence intensity of ROS significantly decreased by 66.9%, 77.2% (P<0.01), respectively. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) contents increased by 72.0% and 50.0%, respectively. Compared to that in the model group, the malondialdehyde (MDA) content decreased by 42.6% (P<0.01) significantly. HPOS at a concentration of 0.8 mg/mL significantly inhibited p53, p21, and p16 expression in the p53/p21/p16 pathway (P<0.01). Thus, HPOS ameliorates the H₂O₂-induced aging of MRC-5 cells by inhibiting apoptosis and oxidative stress damage along with regulating the p53/p21/p16 pathway, thereby providing a scientific basis for the development of HPOS as a functional food ingredient that acts against aging.
- pectin oligosaccharides from hawthorn,
- aging,
- cell apoptosis,
- antioxidant capacity,
- p53/p21/p16 pathway

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References

[1]	LÓPEZ-OTÍN C, BLASCO M A, PARTRIDGE L, et al. Hallmarks of aging:An expanding universe[J]. Cell,2023,186(2):243−278. doi: 10.1016/j.cell.2022.11.001
[2]	HOSSEINI L, FAROKHI-SISAKHT F, BADALZADEH R, et al. Nicotinamide mononucleotide and melatonin alleviate aging-induced cognitive impairment via modulation of mitochondrial function and apoptosis in the prefrontal cortex and hippocampus[J]. Neuroscience,2019,423:29−37. doi: 10.1016/j.neuroscience.2019.09.037
[3]	ZHANG J, CHEN Z W, YU H X, et al. Anti-aging effects of a functional food via the action of gut microbiota and metabolites in aging mice[J]. Aging-US,2021,13(13):17880−17900. doi: 10.18632/aging.202873
[4]	LI R Y, LUAN F, ZHAO Y Y, et al. Crataegus pinnatifida:A botanical, ethnopharmacological, phytochemical, and pharmacological overview[J]. Journal of Ethnopharmacology,2023,301:115819. doi: 10.1016/j.jep.2022.115819
[5]	WANG X X, LI X, LI L Y, et al. Hawthorn fruit extract ameliorates H₂O₂-induced oxidative damage in neuronal PC12 cells and prolongs the lifespan of Caenorhabditis elegans via the IIS signaling pathway[J]. Food and Function,2022,13(20):10680−10694. doi: 10.1039/D2FO01657E
[6]	ZHANG S Y, SUN X L, YANG X L, et al. Botany, traditional uses, phytochemistry and pharmacological activity of Crataegus pinnatifida (Chinese hawthorn):A review[J]. Journal of Pharmacy and Pharmacology,2022,74(11):1507−1545. doi: 10.1093/jpp/rgac050
[7]	GUO Q B, DU J H, JIANG Y, et al. Pectic polysaccharides from hawthorn:Physicochemical and partial structural characterization[J]. Food Hydrocolloids,2019,90:146−153. doi: 10.1016/j.foodhyd.2018.10.011
[8]	MIAO J, LI X, FAN Y, et al. Effect of different solvents on the chemical composition, antioxidant activity and alpha-glucosidase inhibitory activity of hawthorn extracts[J]. International Journal of Food Science and Technology,2016,51(5):1244−1251. doi: 10.1111/ijfs.13076
[9]	LU M, ZHANG L, PAN J, et al. Advances in the study of the vascular protective effects and molecular mechanisms of hawthorn (Crataegus anamesa Sarg.) extracts in cardiovascular diseases[J]. Food and Function,2023,14(13):5870−5890. doi: 10.1039/D3FO01688A
[10]	ZHANG X, HAN Y, HUANG W, et al. The influence of the gut microbiota on the bioavailability of oral drugs[J]. Acta Pharmaceutica Sinica B,2021,11(7):1789−1812. doi: 10.1016/j.apsb.2020.09.013
[11]	LI T P, LI S H, DU L J, et al. Effects of haw pectic oligosaccharide on lipid metabolism and oxidative stress in experimental hyperlipidemia mice induced by high-fat diet[J]. Food Chemistry,2010,121:1010−1013. doi: 10.1016/j.foodchem.2010.01.039
[12]	ZHU R G, SUN Y D, LI T P, et al. Comparative effects of hawthorn (Crataegus pinnatifida Bunge) pectin and pectin hydrolyzates on the cholesterol homeostasis of hamsters fed high-cholesterol diets[J]. Chemico-Biological Interactions,2015,238:42−47. doi: 10.1016/j.cbi.2015.06.006
[13]	YANG S Q, WU C X, YAN Q J, et al. Nondigestible functional oligosaccharides:Enzymatic production and food applications for intestinal health[J]. Annual Review of Food Science and Technology,2023,14:297−322. doi: 10.1146/annurev-food-052720-114503
[14]	NERI-NUMA I A, PASTORE G M. Novel insights into prebiotic properties on human health:A review[J]. Food Research International,2020,131:108973. doi: 10.1016/j.foodres.2019.108973
[15]	ZHU D, YAN Q J, LIU J, et al. Can functional oligosaccharides reduce the risk of diabetes mellitus?[J]. FASEB Journal,2019,33(11):11655−11667. doi: 10.1096/fj.201802802RRR
[16]	XU Q X, FU Q, LI Z, et al. The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice[J]. Nature Metabolism,2021,3(12):1706−1726. doi: 10.1038/s42255-021-00491-8
[17]	LIU X Y, LIU D, LIN G P, et al. Anti-ageing and antioxidant effects of sulfate oligosaccharides from green algae Ulva lactuca and Enteromorpha prolifera in SAMP8 mice[J]. International Journal of Biological Macromolecules,2019,139:342−351. doi: 10.1016/j.ijbiomac.2019.07.195
[18]	TUSI S K, KHALAJ L, ASHABI G, et al. Alginate oligosaccharide protects against endoplasmic reticulum- and mitochondrial-mediated apoptotic cell death and oxidative stress[J]. Biomaterials,2011,32(23):5438−5458. doi: 10.1016/j.biomaterials.2011.04.024
[19]	牟婕, 冯文静, 毛拥军. 甘露糖醛酸寡糖对过氧化氢诱导的衰老心肌细胞的影响[J]. 青岛大学学报(医学版),2019,55(4):411−414,418. [MOU J, FENG W J, MAO Y J. Effect of mannuronic acid oligosaccharides on hydrogen peroxide-induced cardiomyocyte senescence[J]. Journal of Qingdao University (Medical Sciences),2019,55(4):411−414,418.] MOU J, FENG W J, MAO Y J. Effect of mannuronic acid oligosaccharides on hydrogen peroxide-induced cardiomyocyte senescence[J]. Journal of Qingdao University （Medical Sciences）, 2019, 55（4）: 411−414,418.
[20]	LI T, YANG S Q, LIU X Y, et al. Dietary neoagarotetraose extends lifespan and impedes brain aging in mice via regulation of microbiota-gut-brain axis[J]. Journal of Advanced Research,2023,52:119−134. doi: 10.1016/j.jare.2023.04.014
[21]	李涛, 刘晓艳, 王楠楠, 等. β-甘露聚糖酶部分水解香豆胶对自然衰老小鼠的抗衰老作用[J]. 食品科学,2022,43(9):111−119. [LI T, LIU X Y, WANG N N, et al. Anti-aging effect of partially hydrolyzed fenugreek gum by β-mannanase on naturally aging mice[J]. Food Science,2022,43(9):111−119.] LI T, LIU X Y, WANG N N, et al. Anti-aging effect of partially hydrolyzed fenugreek gum by β-mannanase on naturally aging mice[J]. Food Science, 2022, 43（9）: 111−119.
[22]	李涛, 许云聪, 陈峻波, 等. 部分水解瓜尔豆胶对阿尔茨海默病小鼠学习记忆能力的改善作用[J]. 食品科学技术学报,2023,41(5):34−44. [LI T, XU Y C, CHEN J B, et al. Effect of partially hydrolyzed guar gum on learning and memory ability of Alzheimer's disease mice[J]. Journal of Food Science and Technology,2023,41(5):34−44.] doi: 10.12301/spxb202300039 LI T, XU Y C, CHEN J B, et al. Effect of partially hydrolyzed guar gum on learning and memory ability of Alzheimer's disease mice[J]. Journal of Food Science and Technology, 2023, 41（5）: 34−44. doi: 10.12301/spxb202300039
[23]	LI Q, WANG M, ZHANG Y, et al. Pectin-derived oligogalacturonic acids ameliorate high-fat diet-induced obesity in mice by regulating gut microbiota and inflammation[J]. Journal of Functional Foods,2024,112:105928. doi: 10.1016/j.jff.2023.105928
[24]	LI S H, LI T P, JIA Y F, et al. Fractionation and structural characterization of haw pectin oligosaccharides[J]. European Food Research and Technology,2011,233:731−734. doi: 10.1007/s00217-011-1568-8
[25]	LI T, LI Y X, YAN Q J, et al. Co-treatment of nicotinamide mononucleotide and neoagarooligosaccharide mitigates aging-induced cognitive impairment by promoting mitochondrial dynamics[J]. Journal of Functional Foods,2024,112:105922. doi: 10.1016/j.jff.2023.105922
[26]	CHEN P X, ZHANG L Y, CHEN Y, et al. Mitochondrial stress and aging:Lessons from C. elegans[J]. Seminars in Cell & Developmental Biology,2024,154:69−76.
[27]	OGRODNIK M. Cellular aging beyond cellular senescence:Markers of senescence prior to cell cycle arrest in vitro and in vivo[J]. Aging Cell,2021,20(4):e13338. doi: 10.1111/acel.13338
[28]	LI L, ZHANG H, CHEN B B, et al. BaZiBuShen alleviates cognitive deficits and regulates Sirt6/NRF2/HO-1 and Sirt6/P53-PGC-1 alpha-TERT signaling pathways in aging mice[J]. Journal of Ethnopharmacology,2022,282:114653. doi: 10.1016/j.jep.2021.114653
[29]	WANG C C, WANG D, XU J, et al. DHA enriched phospholipids with different polar groups (PC and PS) had different improvements on MPTP-induced mice with Parkinson’s disease[J]. Journal of Functional Foods,2018,45:417−426. doi: 10.1016/j.jff.2018.04.017
[30]	DIMRI G P, LEE X, BASILE G, et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo[J]. PNAS,1995,92(20):9363−9367. doi: 10.1073/pnas.92.20.9363
[31]	GREEN D R, REED J C. Mitochondria and apoptosis[J]. Science,1998,281:1309−1312. doi: 10.1126/science.281.5381.1309
[32]	KUMAR P, LIU C, SULIBURK J, et al. Supplementing glycine and n-acetylcysteine (GlyNAC) in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, physical function, and aging hallmarks:A randomized clinical trial[J]. Journals of Gerontology Series a-Biological Sciences and Medical Sciences,2023,78(1):75−89. doi: 10.1093/gerona/glac135
[33]	BHATT M P, LIM Y C, KIM Y M, et al. C-peptide activates AMPKα and prevents ROS-mediated mitochondrial fission and endothelial apoptosis in diabetes[J]. Diabetes,2013,62:3851−3862. doi: 10.2337/db13-0039
[34]	LU J T, QI C L, LIMBU S M, et al. Dietary mannan oligosaccharide (MOS) improves growth performance, antioxidant capacity, non-specific immunity and intestinal histology of juvenile Chinese mitten crabs (Eriocheir sinensis)[J]. Aquaculture,2019,510(15):337−346.
[35]	DENG X J, LIANG X H, YANG H Y, et al. Nicotinamide mononucleotide (NMN) protects bEnd. 3 cells against H₂O₂-induced damage via NAMPT and the NF-κB p65 signalling pathway[J]. FEBS Open Bio,2021,11:866−879. doi: 10.1002/2211-5463.13067
[36]	MORRONE M D, de ASSIS A M, da ROCHA R F, et al. Passiflora manicata (Juss.) aqueous leaf extract protects against reactive oxygen species and protein glycation in vitro and exvivo models[J]. Food and Chemical Toxicology,2013,60:45−51. doi: 10.1016/j.fct.2013.07.028
[37]	SHADAB M, AGRAWAL D K, ASLAM M, et al. Occupational health hazards among sewage workers:Oxidative stress and deranged lung functions[J]. Journal of Clinical and Diagnostic Research,2014,8(4):BC11−BC12.
[38]	YANG F, YI M Q, LIU Y, et al. Glutaredoxin-1 silencing induces cell senescence via p53/p21/p16 signaling axis[J]. Journal of Proteome Research,2018,17(3):1091−1100. doi: 10.1021/acs.jproteome.7b00761
[39]	BAO Y Z, HE X Y, WU W L, et al. Sulfated galactofucan from Sargassum thunbergii induces senescence in human lung cancer A549 cells[J]. Food and Function,2020,11(5):4785−4792. doi: 10.1039/D0FO00699H

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