<i>In vitro</i> Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil

PAN Zhitao; HUA Tingyu; WANG Xiaoyun; SHEN Yi; ZHANG Xunzhen; HUANG Shaotong; DUAN Hanying; WANG Chao

doi:10.13386/j.issn1002-0306.2024050299

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PAN Zhitao, HUA Tingyu, WANG Xiaoyun, et al. In vitro Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil[J]. Science and Technology of Food Industry, 2025, 46(8): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050299.

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In vitro Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil

1.
College of Life Science and Technology, Jinan University, Guangzhou 510630, China
2.
Guangzhou Tongshanchang Biotechnology Co., Ltd., Guangzhou 511445, China
3.
Guangdong Xinle Food Co., Ltd., Chaozhou 515638, China

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Received Date: June 05, 2023
Available Online: February 09, 2025

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Abstract

Objective: Exploring the in vitro hypoglycemic, hypolipidemic and antioxidant activity of Pericarpium Citri Reticulatae volatile oil and dark tea/Pericarpium Citri Reticulatae extract. Methods: The volatile oil components of Pericarpium Citri Reticulatae were analyzed by gas chromatography–mass spectrometry (GC-MS), and then the effects of Pericarpium Citri Reticulatae, dark tea, dark tea with Pericarpium Citri Reticulatae extract and Pericarpium Citri Reticulatae volatile oil on in vitro α-glucosidase, pancreatic α-amylase, pancreatic lipase activity and the DPPH and ABTS⁺ free radicals scavenging capacity were studied with acarbose, orlistat and vitamin C (V_C) as controls. Results: Limonene was the main component of Pericarpium Citri Reticulatae volatile oil, accounting for 62.89% of the total relative content. The inhibition rate of α-glucosidase of dark tea with Pericarpium Citri Reticulatae+volatile oil group (0.1 mg/mL) was 83.38%±1.82%, and the IC₅₀ value was: acarbose < dark tea < dark tea with Pericarpium Citri Reticulatae+volatile oil < dark tea with Pericarpium Citri Reticulatae < Pericarpium Citri Reticulatae. The IC₅₀ value of all groups on pancreatic lipase inhibition rate was in the range of 1.77~2.60 mg/mL. The DPPH free radical scavenging capacity of dark tea with Pericarpium Citri Reticulatae+volatile oil group at a concentration of 0.2 mg/mL was 94.85%±1.39%. The IC₅₀ value of ABTS cation radical scavenging capacity was 27.62~210.90 μg/mL. The IC₅₀ value of DPPH free radical scavenging capacity was 46.73~68.39 μg/mL. The inhibition rate of pancreatic α-amylase activity in the all groups of dark tea with Pericarpium Citri Reticulatae was less than 30 %. Conclusion: The extract of dark tea with Pericarpium Citri Reticulatae has good antioxidant capacity and in vitro hypoglycemic and lipid-lowering activity, and the addition of volatile oil of Pericarpium Citri Reticulatae can improve the antioxidant, in vitro hypoglycemic and lipid-lowering ability of the extract of dark tea with Pericarpium Citri Reticulatae. This study provides a theoretical basis for the development of health care products related to hypoglycemic, hypolipidemic and antioxidation effects of dark tea with Pericarpium Citri Reticulatae.

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