PAN Zhitao, HUA Tingyu, WANG Xiaoyun, et al. In Vitro Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil[J]. Science and Technology of Food Industry, 2025, 46(8): 341−350. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050299.
Citation: PAN Zhitao, HUA Tingyu, WANG Xiaoyun, et al. In Vitro Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil[J]. Science and Technology of Food Industry, 2025, 46(8): 341−350. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050299.

In Vitro Hypoglycemic, Hypolipidemic Activities and Antioxidant Capacity of Dark Tea and Pericarpium Citri Reticulatae Extract with Pericarpium Citri Reticulatae Volatile Oil

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  • Received Date: June 05, 2024
  • Available Online: February 09, 2025
  • Objective: Exploring the in vitro hypoglycemic, hypolipidemic and antioxidant activity of Pericarpium Citri Reticulatae volatile oil and dark tea/Pericarpium Citri Reticulatae extract. Methods: The volatile oil components of Pericarpium Citri Reticulatae were analyzed by gas chromatography-mass spectrometry (GC-MS), and then the effects of Pericarpium Citri Reticulatae, dark tea, dark tea with Pericarpium Citri Reticulatae extract and Pericarpium Citri Reticulatae volatile oil on in vitro α-glucosidase, pancreatic α-amylase, pancreatic lipase activity and the DPPH and ABTS+ free radicals scavenging capacity were studied with acarbose, orlistat and vitamin C (VC) as controls. Results: Limonene was the main component of Pericarpium Citri Reticulatae volatile oil, accounting for 62.89% of the total relative content. The inhibition rate of α-glucosidase of dark tea with Pericarpium Citri Reticulatae+volatile oil group (0.1 mg/mL) was 83.38%±1.82%, and the IC50 value was: acarbose<dark tea<dark tea with Pericarpium Citri Reticulatae+volatile oil<dark tea with Pericarpium Citri Reticulatae<Pericarpium Citri Reticulatae. The IC50 value of all groups on pancreatic lipase inhibition rate was in the range of 1.77~2.60 mg/mL. The DPPH free radical scavenging capacity of dark tea with Pericarpium Citri Reticulatae+volatile oil group at a concentration of 0.2 mg/mL was 94.85%±1.39%. The IC50 value of ABTS cation radical scavenging capacity was 27.62~210.90 μg/mL. The IC50 value of DPPH free radical scavenging capacity was 46.73~68.39 μg/mL. The inhibition rate of pancreatic α-amylase activity in the all groups of dark tea with Pericarpium Citri Reticulatae was less than 30 %. Conclusion: The extract of dark tea with Pericarpium Citri Reticulatae has good antioxidant capacity and in vitro hypoglycemic and lipid-lowering activity, and the addition of volatile oil of Pericarpium Citri Reticulatae can improve the antioxidant, in vitro hypoglycemic and lipid-lowering ability of the extract of dark tea with Pericarpium Citri Reticulatae. This study provides a theoretical basis for the development of health care products related to hypoglycemic, hypolipidemic and antioxidation effects of dark tea with Pericarpium Citri Reticulatae.
  • [1]
    肖杰, 侯粲, 陈鑫, 等. 发酵陈皮黑茶改善高脂饮食诱导的小鼠糖脂代谢紊乱[J]. 食品科学,2022,43(5):133−142. [XIAO J, HOU C, CHEN X, et al. Dark tea with Pericarpium Citri Reticulatae improves glucose metabolism and lipid metabolism disorders induced by high-fat diet in mice[J]. Food Science,2022,43(5):133−142.] doi: 10.7506/spkx1002-6630-20210316-218

    XIAO J, HOU C, CHEN X, et al. Dark tea with Pericarpium Citri Reticulatae improves glucose metabolism and lipid metabolism disorders induced by high-fat diet in mice[J]. Food Science, 2022, 43(5): 133−142. doi: 10.7506/spkx1002-6630-20210316-218
    [2]
    侯粲, 杜昱光, 王曦, 等. 发酵陈皮黑茶的化学成分差异及体外活性[J]. 食品科学,2020,41(18):226−232. [HOU C, DU Y G, WANG X, et al. Fermented dark tea with Pericarpium Citri Reticulatae:comparison of chemical composition with raw dark tea and bioactivities in vitro[J]. Food Science,2020,41(18):226−232.] doi: 10.7506/spkx1002-6630-20190717-228

    HOU C, DU Y G, WANG X, et al. Fermented dark tea with Pericarpium Citri Reticulatae: comparison of chemical composition with raw dark tea and bioactivities in vitro[J]. Food Science, 2020, 41(18): 226−232. doi: 10.7506/spkx1002-6630-20190717-228
    [3]
    WU T, XU J L, CHEN Y J, et al. Oolong tea polysaccharide and polyphenols prevent obesity development in Sprague-Dawley rats[J]. Food & Nutrition Research, 2018, 62:1599.
    [4]
    李家兴, 王朝霞, 刘升锐, 等. 不同茶树品种黄大茶降糖降脂功效比较分析[J]. 中国茶叶,2024,46(3):41−51. [LI J X, WANG Z X, LIU S R, et al. Comparative analysis of hypoglycemic and lipid-lowering efficacy of large-leaf yellow tea made from different tea cultivars[J]. China Tea,2024,46(3):41−51.]

    LI J X, WANG Z X, LIU S R, et al. Comparative analysis of hypoglycemic and lipid-lowering efficacy of large-leaf yellow tea made from different tea cultivars[J]. China Tea, 2024, 46(3): 41−51.
    [5]
    薛芳, 许占民. 中国药物大全[M]. 北京:人民卫生出版社, 2005. [XUE F, XU Z M. The complete list of chinese medicines[M]. Beijing:People’s Medical Publishing House, 2005.]

    XUE F, XU Z M. The complete list of chinese medicines[M]. Beijing: People’s Medical Publishing House, 2005.
    [6]
    杨秀娟, 巢颖欣, 蔡轶, 等. 新会陈皮化学成分的综合分析测定研究[J]. 中国医院药学杂志,2019(4):348−352. [YANG X J, CHAO Y X, CAI Y, et al. Comprehensive analysis and determination of chemical components in Xinhui Chenpi[J]. Chinese Journal of Hospital Pharmacy,2019(4):348−352.]

    YANG X J, CHAO Y X, CAI Y, et al. Comprehensive analysis and determination of chemical components in Xinhui Chenpi[J]. Chinese Journal of Hospital Pharmacy, 2019(4): 348−352.
    [7]
    严绥平, 于蕾, 申静伟, 等. 陈皮挥发油成分的气-质联用分析及药理作用研究进展[J]. 内蒙古中医药,2007(3):57−58. [YAN S P, YU L, SHEN J W, et al. Progress of gas-mass spectrometry analysis and pharmacological effects of the volatile oil components of Chen Pi[J]. Inner Mongolia Journal of Traditional Chinese Medicine,2007(3):57−58.] doi: 10.3969/j.issn.1006-0979.2007.03.048

    YAN S P, YU L, SHEN J W, et al. Progress of gas-mass spectrometry analysis and pharmacological effects of the volatile oil components of Chen Pi[J]. Inner Mongolia Journal of Traditional Chinese Medicine, 2007(3): 57−58. doi: 10.3969/j.issn.1006-0979.2007.03.048
    [8]
    李俊健, 林锦铭, 高杰贤, 等. 陈皮挥发油提取、成分分析及应用的研究进展[J]. 中国调味品,2021,46(8):169−173. [LI J J, LIN J M, GAO J X, et al. Research progress on extraction, component analysis and application of essential oils from citrus reticulata blanco[J]. China Condiment,2021,46(8):169−173.] doi: 10.3969/j.issn.1000-9973.2021.08.036

    LI J J, LIN J M, GAO J X, et al. Research progress on extraction, component analysis and application of essential oils from citrus reticulata blanco[J]. China Condiment, 2021, 46(8): 169−173. doi: 10.3969/j.issn.1000-9973.2021.08.036
    [9]
    清·赵学敏. 本草纲目拾遗[M]. 本草纲目拾遗, 1963. [Ching Dynasty·ZHAO X M. Gleanings from the Cmpendium of Materia Medica[M]. Gleanings from the Compendium of Materia Medica, 1963.]

    Ching Dynasty·ZHAO X M. Gleanings from the Cmpendium of Materia Medica[M]. Gleanings from the Compendium of Materia Medica, 1963.
    [10]
    李勤, 熊立瑰, 晏玲玲, 等. 安化黑茶的降脂减肥功效及作用机理[J]. 中国茶叶,2023,45(1):6−11. [LI Q, XIONG L H, YAN L L, et al. Effect and mechanism of Anhua dark tea on reducing blood lipid and weight[J]. China Tea,2023,45(1):6−11.] doi: 10.3969/j.issn.1000-3150.2023.01.002

    LI Q, XIONG L H, YAN L L, et al. Effect and mechanism of Anhua dark tea on reducing blood lipid and weight[J]. China Tea, 2023, 45(1): 6−11. doi: 10.3969/j.issn.1000-3150.2023.01.002
    [11]
    GUO J. Research progress on prevention and treatment of glucolipid metabolic disease with integrated traditional Chinese and Western medicine[J]. Chinese Journal of Integrative Medicine,2017,23(6):403−409. doi: 10.1007/s11655-017-2811-3
    [12]
    LI Y T, ZHAO X T, XU M R, et al. Krüppel-like factors in glycolipid metabolic diseases[J]. Molecular Biology Reports,2022,49(8):8145−8152. doi: 10.1007/s11033-022-07565-0
    [13]
    牛亚芊芊, 郭丹杰. 调脂药物的不良反应及其处理措施[J]. 中国全科医学,2016(36):4416−4419. [NIU Y Q Q, GUO D J. Adverse effects of lipid- lowering agents and corresponding management[J]. Chinese General Practice,2016(36):4416−4419.]

    NIU Y Q Q, GUO D J. Adverse effects of lipid- lowering agents and corresponding management[J]. Chinese General Practice, 2016(36): 4416−4419.
    [14]
    易咏梅, 陈瑛. 糖尿病患者降糖药物治疗依从性影响因素临床分析[J]. 基层医学论坛,2013,17(28):3740−3741. [YI Y M, CHEN Y. Clinical analysis of factors influencing adherence to glucose-lowering drug therapy in diabetic patients[J]. The Medical Forum,2013,17(28):3740−3741.] doi: 10.3969/j.issn.1672-1721.2013.28.042

    YI Y M, CHEN Y. Clinical analysis of factors influencing adherence to glucose-lowering drug therapy in diabetic patients[J]. The Medical Forum, 2013, 17(28): 3740−3741. doi: 10.3969/j.issn.1672-1721.2013.28.042
    [15]
    司徒瑞娴, 张诗军, 向爱民, 等. 陈皮黑茶联合二甲双胍治疗2型糖尿病临床研究[J]. 新中医,2019,51(5):176−178. [SI TU D X, ZHANG S J, XIANG A M, et al. Clinical study on dark tea with Pericarpium Citri Reticulatae combined with metforminfor type 2 diabetes mellitus[J]. New Chinese Medicine,2019,51(5):176−178.]

    SI TU D X, ZHANG S J, XIANG A M, et al. Clinical study on dark tea with Pericarpium Citri Reticulatae combined with metforminfor type 2 diabetes mellitus[J]. New Chinese Medicine, 2019, 51(5): 176−178.
    [16]
    司徒瑞娴, 张诗军, 向爱民, 等. 陈皮黑茶联合瑞舒伐他汀治疗高脂血症临床研究[J]. 实用中医药杂志,2019,35(9):1136−1137. [SI TU D X, ZHANG S J, XIANG A M, et al. Clinical study of Pericarpium Citri Reticulatae combined with Rosuvastatin in the treatment of hyperlipidaemia[J]. Journal of Practical Traditional Chinese Medicine,2019,35(9):1136−1137.]

    SI TU D X, ZHANG S J, XIANG A M, et al. Clinical study of Pericarpium Citri Reticulatae combined with Rosuvastatin in the treatment of hyperlipidaemia[J]. Journal of Practical Traditional Chinese Medicine, 2019, 35(9): 1136−1137.
    [17]
    李尚科, 李跑, 杜国荣, 等. 陈皮组成成分及检测方法研究进展[J]. 中国果菜,2019,39(10):36−39,52. [LI S K, LI P, DU G R, et al. Progress in composition and determination methods of Pericarpium Citri Reticulatae[J]. China Fruit & Vegetable,2019,39(10):36−39,52.]

    LI S K, LI P, DU G R, et al. Progress in composition and determination methods of Pericarpium Citri Reticulatae[J]. China Fruit & Vegetable, 2019, 39(10): 36−39,52.
    [18]
    崔佳韵, 梁建芬. 不同年份新会陈皮挥发油的抗氧化活性评价[J]. 食品科技,2019,44(1):98−102. [CUI J Y, LIANG J F. Antioxidant capacities of essential oils in Xinhui Pericarpium Citri Reticulatae at different storage period[J]. Food Science and Technology,2019,44(1):98−102.]

    CUI J Y, LIANG J F. Antioxidant capacities of essential oils in Xinhui Pericarpium Citri Reticulatae at different storage period[J]. Food Science and Technology, 2019, 44(1): 98−102.
    [19]
    王娟. 陈皮挥发油对大鼠慢性肺阻的疗效及肺巨噬细胞应答机制[D]. 南充:西华师范大学, 2021. [WANG J. Effect of volatile oil from Citrus Reticulata on chronic pulmonary obstruction in rats and the mechanism of macrophage response[D]. Nanchong:China West Normal University, 2021.]

    WANG J. Effect of volatile oil from Citrus Reticulata on chronic pulmonary obstruction in rats and the mechanism of macrophage response[D]. Nanchong: China West Normal University, 2021.
    [20]
    张丽娟. 云南野生香薷油调节血脂及对子代小鼠学习记忆的影响[D]. 昆明:昆明医学院, 2011. [ZHANG L J. Effects of Yunnan wild eIlsholtzia oil on regulating blood lipids of mice and on learning and memory abilities of their offsprings[D]. Kunming:Kunming Medical University, 2011.]

    ZHANG L J. Effects of Yunnan wild eIlsholtzia oil on regulating blood lipids of mice and on learning and memory abilities of their offsprings[D]. Kunming: Kunming Medical University, 2011.
    [21]
    刘沁如, 向茗, 瞿昊宇, 等. 陈皮黑茶颗粒提取工艺条件对降糖降脂活性成分的影响[J]. 亚太传统医药,2021,17(12):66−73. [LIU Q R, XIANG M, QU H Y, et al. Effects of extraction conditions of tangerine peel black tea granules on hypoglycemic and lipid lowering active components[J]. Asia-Pacific Traditional Medicine,2021,17(12):66−73.]

    LIU Q R, XIANG M, QU H Y, et al. Effects of extraction conditions of tangerine peel black tea granules on hypoglycemic and lipid lowering active components[J]. Asia-Pacific Traditional Medicine, 2021, 17(12): 66−73.
    [22]
    王亚凤, 张祖湘, 何瑞杰, 等. 黄精地上部位化学成分及其胰脂肪酶抑制活性研究[J]. 天然产物研究与开发,2020,32(11):1811−1817. [WANG Y F, ZHANG Z X, HE R J, et al. Study on the chemical constituents of the aerial parts of Polygonatumsibiricum and its pancreatic lipase inhibitory activity[J]. Natural Product Research and Development,2020,32(11):1811−1817.]

    WANG Y F, ZHANG Z X, HE R J, et al. Study on the chemical constituents of the aerial parts of Polygonatumsibiricum and its pancreatic lipase inhibitory activity[J]. Natural Product Research and Development, 2020, 32(11): 1811−1817.
    [23]
    LELAND L. Type 2 diabetes market[J]. Nature Reviews Drug Discovery,2005,4(5):367−368. doi: 10.1038/nrd1723
    [24]
    冯诗华. 绿球藻多糖的抗氧化、抗炎及体外降糖降脂活性探究[D]. 太原:山西大学, 2023. [FENG S H. Investigation of antioxidant, anti-inflammatory and in vitro hypoglycemic and hypolipidemic activities of Chlorococcum sphacosum GD polysaccharide[D]. Taiyuan:Shanxi University, 2023.]

    FENG S H. Investigation of antioxidant, anti-inflammatory and in vitro hypoglycemic and hypolipidemic activities of Chlorococcum sphacosum GD polysaccharide[D]. Taiyuan: Shanxi University, 2023.
    [25]
    CARDENAS F, ALVAREZ E, CASTRO-ALVAREZ M S D, et al. Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases[J]. Journal of Molecular Catalysis B Enzymatic,2001,14(4-6):111−123. doi: 10.1016/S1381-1177(00)00244-7
    [26]
    SINGH R, DEVI S, GOLLEN R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia:larger-than-life[J]. Diabetes/Metabolism Research and Reviews,2015,31(2):113−126. doi: 10.1002/dmrr.2558
    [27]
    华爽, 吕明慧, 刘倩颖, 等. 糖脂代谢病的发病机制:多重打击学说[J]. 世界中医药, 2019, (3):638-44. [HUA S, LÜ M H, LIU Q Y, et al. The multiple-hit pathogenesis of glucolipid metabolic disorders[J]. World Chinese Medicine, 2019, (3):638-644.]

    HUA S, LÜ M H, LIU Q Y, et al. The multiple-hit pathogenesis of glucolipid metabolic disorders[J]. World Chinese Medicine, 2019, (3): 638-644.
    [28]
    HASSAN W, RONGYIN G, DAOUD A, et al. Reduced oxidative stress contributes to the lipid lowering effects of isoquercitrin in free fatty acids induced hepatocytes[J]. Oxidative Medicine and Cellular Longevity,2014,2014(1):313602.
    [29]
    CICERO A F G, COLLETTI A, BELLENTANI S. Nutraceutical approach to non-alcoholic fatty liver disease (NAFLD):The available clinical evidence[J]. Nutrients,2018,10(9):1153. doi: 10.3390/nu10091153
    [30]
    ABENAVOLI L, GRECO M, MILIC N, et al. Effect of mediterranean diet and antioxidant formulation in non-alcoholic fatty liver disease:A randomized study[J]. Nutrients,2017,9(8):870. doi: 10.3390/nu9080870
    [31]
    DOUGHARI J H, BAZZA M J. Phytochemistry, GC-MS analysis, antioxidant and antibacterial potentials of limonene isolated from pericarp of citrus sinensis[J]. International Journal of Microbiology and Biotechnology,2020,5(1):22−27. doi: 10.11648/j.ijmb.20200501.14
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