BI Yinghao, JIA Airong, ZHANG Miansong, et al. Effects of Konjac Glucomannan and Whey Protein Complex on Stability and Slow Release Properties of Vitamin C W1/O/W2 Multiple Emulsion[J]. Science and Technology of Food Industry, 2025, 46(10): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050112.
Citation: BI Yinghao, JIA Airong, ZHANG Miansong, et al. Effects of Konjac Glucomannan and Whey Protein Complex on Stability and Slow Release Properties of Vitamin C W1/O/W2 Multiple Emulsion[J]. Science and Technology of Food Industry, 2025, 46(10): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050112.

Effects of Konjac Glucomannan and Whey Protein Complex on Stability and Slow Release Properties of Vitamin C W1/O/W2 Multiple Emulsion

More Information
  • Received Date: May 12, 2024
  • Available Online: March 20, 2025
  • To study the effects of konjac glucomannan and whey protein on the stability and slow release properties of vitamin C multiple emulsion, and to provide reference for the application of vitamin C multiple emulsion. W1/O/W2 multiple emulsion loaded with vitamin C was prepared by mixing konjac glucomannan and whey protein. The effects of various mass fractions of KGM and WP on the centrifugation stability index, centrifugal precipitation rate, loading rate, particle size, Zeta potential, rheological properties, vitamin C retention rate and release rate of multiple emulsion were investigated. The results showed that the multiple emulsion exhibited good stability and slow release properties when the mass fraction of KGM was 0.10% and WP quality score was 10.00%. At this point, the droplet distributed equally, the particle size was 64.38 μm, and the Zeta potential was −46.50 mV. The loading rate of vitamin C was reached 93.24%. No obvious stratification occurred after 20 d when the multiple emulsion was stored at 4, 25 and 50 ℃. After simulated digestion, the release rate of vitamin C in the emulsion was 85.64%. The above results confirmed that KGM and WP combination has the potential ability to stabilize vitamin C W1/O/W2 multiple emulsion.
  • [1]
    CARITÁ A C, FONSECA-SANTOS B, SHULTZ J D, et al. Vitamin C:One compound, several uses. Advances for delivery, efficiency and stability[J]. Nanomedicine,2020,24:102117. doi: 10.1016/j.nano.2019.102117
    [2]
    DOSEDĚL M, JIRKOVSKÝ E, MACÁKOVÁ K, et al. Vitamin C-sources, physiological role, kinetics, deficiency, use, toxicity, and determination[J]. Nutrients,2021,13(2):615. doi: 10.3390/nu13020615
    [3]
    ARILLA E, MARTÍNEZ-MONZÓ J, CODOÑER-FRANCH P, et al. Stability of vitamin C, carotenoids, phenols, and antioxidant capacity of pasteurised orange juice with resistant maltodextrin storage[J]. Food Science and Technology International,2024,30(1):18−29. doi: 10.1177/10820132221124200
    [4]
    KAŹMIERCZAK-BARAńSKA J, BOGUSZEWSKA K, ADAMUS-GRABICKA A, et al. Two faces of vitamin C-antioxidative and pro-oxidative agent[J]. Nutrients,2020,12(5):1501. doi: 10.3390/nu12051501
    [5]
    FERNÁNDEZ-LUQUEÑO F, MEDINA-PÉREZ G, PÉREZ-SOTO E, et al. Bioactive compounds of opuntia spp. acid fruits:Micro and nano-emulsified extracts and applications in nutraceutical foods[J]. Molecules,2021,26(21):6429. doi: 10.3390/molecules26216429
    [6]
    TAN Y, MCCLEMENTS D J. Plant-based colloidal delivery systems for bioactives[J]. Molecules,2021,26(22):6895. doi: 10.3390/molecules26226895
    [7]
    陈松, 张国芳, 张彤, 等. 基于三种多糖与酪蛋白复配制备W/O/W型乳液及其包封红景天苷效果的研究[J]. 食品工业科技,2022,43(19):137−145. [CHEN Song, ZHANG Guofang, ZHANG Tong, et al. Study on the preparation of W/O/W emulsion based on three polysaccharides mixed with casein and its encapsulation effect of salidroside[J]. Science and Technology of Food Industry,2022,43(19):137−145.]

    CHEN Song, ZHANG Guofang, ZHANG Tong, et al. Study on the preparation of W/O/W emulsion based on three polysaccharides mixed with casein and its encapsulation effect of salidroside[J]. Science and Technology of Food Industry, 2022, 43(19): 137−145.
    [8]
    WANG X, WANG S J, NAN Y, et al. The effects of oil type and crystallization temperature on the physical properties of vitamin C-loaded oleogels prepared by an emulsion-templated approach[J]. Food & Function,2020,11(9):8028−8037.
    [9]
    KLOJDOVÁ I, STATHOPOULOS C. W/O/W multiple emulsions:A novel trend in functional ice cream preparations?[J]. Food Chemistry X,2022,16:100451. doi: 10.1016/j.fochx.2022.100451
    [10]
    ZHENG B Y, LI X Y, HAO J, et al. Meat systems produced with Monascus pigment water-in-oil-in-water multiple emulsion as pork fat replacers[J]. Food Chemistry,2023,402:134080. doi: 10.1016/j.foodchem.2022.134080
    [11]
    YE S, ZONGO A W, SHAH B R, et al. Konjac glucomannan (KGM), deacetylated KGM (Da-KGM), and degraded KGM derivatives:A special focus on colloidal nutrition[J]. Journal of Agricultural and Food Chemistry,2021,69(44):12921−12932. doi: 10.1021/acs.jafc.1c03647
    [12]
    WARESINDO W X, PRIYANTO A, SIHOMBING Y A, et al. Konjac glucomannan-based hydrogels with health-promoting effects for potential edible electronics applications:A mini-review[J]. International Journal of Biological Macromolecules,2023,248:125888. doi: 10.1016/j.ijbiomac.2023.125888
    [13]
    ZHANG Y T, TONG C L, CHEN Y Y, et al. Advances in the construction and application of konjac glucomannan-based delivery systems[J]. International Journal of Biological Macromolecules, 2024, 262(Pt 1):129940.
    [14]
    KONG F H, KANG S M, ZHANG J, et al. The non-covalent interactions between whey protein and various food functional ingredients[J]. Food Chemistry,2022,394:133455. doi: 10.1016/j.foodchem.2022.133455
    [15]
    DU Q W, TANG J J, XU M J, et al. Whey protein and maltodextrin-stabilized oil-in-water emulsions:Effects of dextrose equivalent[J]. Food Chemistry,2021,339:128094. doi: 10.1016/j.foodchem.2020.128094
    [16]
    符叶, 周庄, 王大鸷. 温敏性W/O/W型多重乳液的制备及其对维生素C的控释性[J]. 大连工业大学学报,2022,41(4):269−274. [FU Ye, ZHOU Zhuang, WANG Dazhi. Preparing a novel type of W/O/W multiple emulsion with thermal-reversible properties for controlled release of vitamin C[J]. Journal of Dalian Polytechnic University,2022,41(4):269−274.]

    FU Ye, ZHOU Zhuang, WANG Dazhi. Preparing a novel type of W/O/W multiple emulsion with thermal-reversible properties for controlled release of vitamin C[J]. Journal of Dalian Polytechnic University, 2022, 41(4): 269−274.
    [17]
    WANG Z T, TANG W X, SUN Z L, et al. An innovative pickering W/O/W nanoemulsion co-encapsulating hydrophilic lysozyme and hydrophobic perilla leaf oil for extending shelf life of fish products[J]. Food Chemistry,2024,439:138074. doi: 10.1016/j.foodchem.2023.138074
    [18]
    LOU S R, NI X W, XIAO W L, et al. Physical stability, microstructure and antimicrobial properties of konjac glucomannan coatings enriched with Litsea cubeba essential oil nanoemulsion and its effect on citruses preservation[J]. International Journal of Biological Macromolecules, 2024, 256(Pt 1):128306.
    [19]
    XU W, NING Y L, SUN Y Y, et al. Reversibility of freeze-thaw/re-emulsification on pickering emulsion stabilized with gliadin/sodium caseinate nanoparticles and konjac glucomannan[J]. International Journal of Biological Macromolecules,2023,233:123653. doi: 10.1016/j.ijbiomac.2023.123653
    [20]
    LIU N, LIN P, ZHANG K, et al. Konjac glucomannan promoted fabrication of diacylglycerol oil-based oleogels through emulsion-templated approach:Comparison with triacylglycerol oleogels[J]. International Journal of Biological Macromolecules,2023,251:126420. doi: 10.1016/j.ijbiomac.2023.126420
    [21]
    HAN Q, WANG H L, ZHOU T X, et al. Ultrastable emulsion stabilized by the Konjac Glucomannan-Xanthan gum complex[J]. ACS Omega,2023,8(34):31344−31352. doi: 10.1021/acsomega.3c03796
    [22]
    ZHUANG H, LI X Q, WU S J, et al. Fabrication of grape seed proanthocyanidin-loaded W/O/W emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate with konjac glucomannan:Structure, stability, andin vitro digestion[J]. Food Chemistry,2023,418:135975. doi: 10.1016/j.foodchem.2023.135975
    [23]
    WANG R J, MA C L, YAN H T, et al. Preparation and characterization of GX-50 and vitamin C co-encapsulated microcapsules by a water-in-oil-in-water (W1/O/W2) double emulsion-complex coacervation method[J]. Langmuir,2023,39(39):13863−13875. doi: 10.1021/acs.langmuir.3c01360
    [24]
    ZHOU X, ZONG X X, WANG S L, et al. Emulsified blend film based on konjac glucomannan/carrageenan/ camellia oil:Physical, structural, and water barrier properties[J]. Carbohydrate Polymers,2021,251:117100. doi: 10.1016/j.carbpol.2020.117100
    [25]
    CHEN J F, GUO J, LIU S H, et al. Zein particle-stabilized water-in-water emulsion as a vehicle for hydrophilic bioactive compound loading of riboflavin[J]. Journal of Agricultural and Food Chemistry,2019,67(35):9926−9933. doi: 10.1021/acs.jafc.9b02415
    [26]
    SUN Y, TANG W T, PU C F, et al. Improved stability of liposome-stabilized emulsions as a co-encapsulation delivery system for vitamin B2, vitamin E and β-carotene[J]. Food Function,2022,13(5):2966−2984. doi: 10.1039/D1FO03617C
    [27]
    廖静如, 杜冰, 刘志伟, 等. 超声辅助优化制备壳寡糖-果胶稳定的Pickering乳液工艺及稳定性分析[J]. 食品工业科技, 2023, 44(13):9−20. [LIAO Jingru, DU Bing, LIU Zhiwei, et al. Study on ultrasound-assisted optimization of chitooligosaccharidepectin stabilized pickering emulsion and its stability[J]. Science and Technology of Food Industry, 2023, 44(13):9−20.]

    LIAO Jingru, DU Bing, LIU Zhiwei, et al. Study on ultrasound-assisted optimization of chitooligosaccharidepectin stabilized pickering emulsion and its stability[J]. Science and Technology of Food Industry, 2023, 44(13): 9−20.
    [28]
    PEI Y Q, ZHANG Y Q, DING H, et al. Stability and rheological behavior of mayonnaise-like emulsion co-emulsified by konjac glucomannan and whey protein[J]. Foods,2023,12(15):2907. doi: 10.3390/foods12152907
    [29]
    YAHYA M, SAKTI SCW, FAHMI M Z, et al. Bio-based Pickering emulsifier from mangosteen residues-derived sodium caseinate grafted spherical cellulose nanocrystals:stability, rheological properties and microstructure studies[J]. International Journal of Biological Macromolecules, 2024, 257(Pt 2):128696.
    [30]
    XU W, NING Y L, WANG M Y, et al. Construction of astaxanthin loaded Pickering emulsions gel stabilized by xanthan gum/lysozyme nanoparticles with konjac glucomannan from structure, protection and gastrointestinal digestion perspective[J]. International Journal of Biological Macromolecules,2023,252:126421. doi: 10.1016/j.ijbiomac.2023.126421
    [31]
    AL-MAQTARI QA, GHALEB ADS, MAHDI AA, et al. Stabilization of water-in-oil emulsion of pulicaria jaubertii extract by ultrasonication:fabrication, characterization, and storage stability[J]. Food Chemistry,2021,350:129249. doi: 10.1016/j.foodchem.2021.129249
    [32]
    HUANG X, TU R, SONG H B, et al. Gelatin-EGCG-high methoxyl pectin ternary complex stabilized W1/O/W2 double emulsions loaded with vitamin C:Formation, structure, stability, in vitro gastrointestinal digestion[J]. International Journal of Biological Macromolecules,2022,216:891−905. doi: 10.1016/j.ijbiomac.2022.07.210
    [33]
    JIANG H, HU X F, JIANG W J, et al. Water-in-oil pickering emulsions stabilized by hydrophobized protein microspheres[J]. Langmuir,2022,38(40):12273−12280. doi: 10.1021/acs.langmuir.2c01904
    [34]
    DAI Y P, LU X L, LI R Y, et al. Fabrication and characterization of W/O/W emulgels by sipunculus nudus salt-soluble proteins:co-encapsulation of vitamin C and β-carotene[J]. Foods,2022,11(18):2720. doi: 10.3390/foods11182720
    [35]
    LIU Z, SHEN R, YANG X B, et al. Characterization of a novel konjac glucomannan film incorporated with pickering emulsions:Effect of the emulsion particle sizes[J]. International Journal of Biological Macromolecules,2021,179:377−387. doi: 10.1016/j.ijbiomac.2021.02.188
    [36]
    BASHIR O, AMIN T, HUSSAIN S Z, et al. Development, characterization and use of rosemary essential oil loaded water-chestnut starch based nanoemulsion coatings for enhancing post-harvest quality of apples var. Golden delicious[J]. Current Research in Food Science,2023,7:100570. doi: 10.1016/j.crfs.2023.100570
    [37]
    MARTÍNEZ-NAVARRETE N, GARCÍA-MARTÍNEZ E, CAMACHO MDM. Characterization of the orange juice powder co-product for its valorization as a food ingredient[J]. Foods,2022,12(1):97. doi: 10.3390/foods12010097
    [38]
    XU Z, ZHANG X Y, WU X X, et al. Co-delivery of vitamin C and β-carotene in W/O/W emulsions stabilized by modified aggregated insoluble soybean protein hydrolysate-xanthan gum complexes[J]. International Journal of Biological Macromolecules, 2024, 261(Pt 2):129855.
    [39]
    PAN Z B, ZHONG W Q, XU J T, et al. Effects of oregano essential oil Pickering emulsion and ZnO nanoparticles on the properties and antibacterial activity of konjac glucomannan/carboxymethyl chitosan nanocomposite films[J]. RSC Advances,2024,14(10):6548−6556. doi: 10.1039/D3RA07845K
    [40]
    LIMAMPAI T, IMPAPRASERT R, SUNTORNSUK W. Influence of some hydrocolloids and sterilization conditions on the physical properties of texture-modified foods developed for the swallow training of dysphagia patients[J]. Foods,2023,12(19):3676. doi: 10.3390/foods12193676
    [41]
    DU Y Y, ZHANG S K, SHENG L J, et al. Food packaging films based on ionically crosslinked konjac glucomannan incorporating zein-pectin nanoparticle-stabilized corn germ oil-oregano oil pickering emulsion[J]. Food Chemistry,2023,429:136874. doi: 10.1016/j.foodchem.2023.136874

Catalog

    Article Metrics

    Article views (8) PDF downloads (1) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return