HU Chuanfeng, QIU Wenxing, LIU Xuan, et al. Effect of Different Cooking Methods on the Flavor of Procambarus clarkii[J]. Science and Technology of Food Industry, 2025, 46(9): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050038.
Citation: HU Chuanfeng, QIU Wenxing, LIU Xuan, et al. Effect of Different Cooking Methods on the Flavor of Procambarus clarkii[J]. Science and Technology of Food Industry, 2025, 46(9): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050038.

Effect of Different Cooking Methods on the Flavor of Procambarus clarkii

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  • Received Date: May 06, 2024
  • Available Online: March 02, 2025
  • To investigate the influence of various cooking methods on the flavor profile of Procambarus clarkii (crayfish), a comparative study was conducted employing steaming, boiling, deep-frying, air-frying, microwave irradiation, and roasting as the culinary techniques. The resultant volatile organic compounds (VOCs) were characterized using gas chromatography coupled with ion mobility spectrometry (GC-IMS). Subsequent multivariate statistical analyses were performed, including orthogonal partial least squares discriminant analysis (OPLS-DA) for discriminant modeling and principal component analysis (PCA) for data dimensionality reduction and visualization. The taste attributes of the cooked crayfish were further assessed through the quantification of free amino acids and nucleotides, as well as by employing a sensory evaluation system utilizing an electronic tongue. By quantifying free amino acids and nucleotides, as well as utilizing electronic tongue technology, the subtle changes in the taste profile of Procambarus clarkii were assessed. The findings revealed that across the six distinct cooking methods applied to Procambarus clarkii, a total of 35 volatile compounds were identified, comprising 5 alcohols, 16 aldehydes, 7 ketones, 4 esters, 1 heterocyclic compound, 1 sulfur compound, and 1 hydrocarbon. Significant differences were observed among the culinary methods (P<0.05). Microwave heating, steaming, and frying yielded a more diverse array of aromas, with the fried samples exhibiting the greatest number of aroma-active compounds, such as methyl 2-methylbutanoate and (E)-2-heptenal, which lent a distinctive fatty aroma to the fried Procambarus clarkii, heightening its sensory quality. Boiling, on the other hand, increased umami amino acids and nucleotides while reducing bitter amino acids in the shrimp, reaching a peak equivalents of umami concentration (EUC) of 23.6 g MSG/100 g. The total amount of free amino acids obtained by roasting was the largest, but the content of umami amino acids was the lowest, and the sensory quality was relatively low. Consequently, frying and boiling emerge as the preferred cooking methods for optimizing the flavor profile of Procambarus clarkii.
  • [1]
    温丽敏, 诸永志, 罗章, 等. 响应面法优化小龙虾烤箱辅助烤制工艺[J]. 肉类研究,2023,37(5):39−48. [WEN Limin, ZHU Yongzhi, LUO Zhang, et al. Optimizing the oven roasting of crayfish by response surface methodology[J]. Meat Research,2023,37(5):39−48.]

    WEN Limin, ZHU Yongzhi, LUO Zhang, et al. Optimizing the oven roasting of crayfish by response surface methodology[J]. Meat Research, 2023, 37(5): 39−48.
    [2]
    徐文思, 杨祺福, 赵子龙, 等. 微波熟制对小龙虾营养与风味的影响[J]. 食品与机械,2022,38(2):216−221. [XU Wensi, YANG Qifu, ZHAO Zilong, et al. Study on microwave heating on nutrition and flavor composition of crayfish[J]. Food and Machinery,2022,38(2):216−221.]

    XU Wensi, YANG Qifu, ZHAO Zilong, et al. Study on microwave heating on nutrition and flavor composition of crayfish[J]. Food and Machinery, 2022, 38(2): 216−221.
    [3]
    CAO Y, WU G, ZHANG F, et al. A comparative study of physicochemical and flavor characteristics of chicken nuggets during air frying and deep frying[J]. Journal of the American Oil Chemists' Society,2020,97:901−913. doi: 10.1002/aocs.12376
    [4]
    JOSHY C G, RATHEESH G, NINAN G, et al. Optimizing air-frying process conditions for the development of healthy fish snack using response surface methodology under correlated observations[J]. Journal of Food Science and Technology,2020,57:2651−2658. doi: 10.1007/s13197-020-04301-z
    [5]
    ZHU X, YANG C, SONG Y, et al. Changes provoked by altitudes and cooking methods in physicochemical properties, volatile profile, and sensory characteristics of yak meat[J]. Food Chemistry:X,2023,20:101019.
    [6]
    RAO J W, MENG F B, LI Y C, et al. Effect of cooking methods on the edible, nutritive qualities and volatile flavor compounds of rabbit meat[J]. Journal of the Science of Food and Agriculture,2022,102(10):4218−4228. doi: 10.1002/jsfa.11773
    [7]
    RAZA A, SONG H, RAZA J, et al. Formation of beef-like odorants from glutathione-enriched yeast extract via Maillard reaction[J]. Food & Function,2020,11(10):8583−8601.
    [8]
    SHI S, WANG X, WU X, et al. Effects of four cooking methods on sensory and taste quality of Portunus trituberculatus[J]. Food Science & Nutrition,2020,8:1115−1124.
    [9]
    程荣, 刘瑞玲, 吴伟杰, 等. 电子鼻结合气相色谱-离子迁移谱法鉴别不同干燥方式的膳食虾肉复合果蔬棒风味变化[J]. 食品安全质量检测学报, 2024, 15(9):187-196. [CHENG Rong, LIU Ruilin, WU Weijie, et al. Flavour changes of dietary shrimp composite fruit and vegetable sticks with different drying methods identified by electronic nose combined with gas chromatography-ion mobility spectrometry[J]Journal of Food Safety & Quality, 2024, 15 (9):187-196.]

    CHENG Rong, LIU Ruilin, WU Weijie, et al. Flavour changes of dietary shrimp composite fruit and vegetable sticks with different drying methods identified by electronic nose combined with gas chromatography-ion mobility spectrometry[J]Journal of Food Safety & Quality, 2024, 15 (9): 187-196.
    [10]
    HUANG J B, KONG X W, CHEN Y Y, et al. Assessment of flavor characteristics in snakehead (Ophiocephalus argus Cantor) surimi gels affected by atmospheric cold plasma treatment using GC-IMS[J]. Frontiers in Nutrition,2022,9:1086426.
    [11]
    杜柳, 邱文兴, 刘栋银, 等. 不同热加工方式熟化对克氏原螯虾理化性质和风味的影响[J]. 肉类研究,2023,37(5):49−56. [DU Liu, QIU Wenxing, LIU Dongyin, et al. Effects of different cooking methods on the physicochemical properties and flavor of crawfish (Procambarus clarkia)[J]. Meat Research,2023,37(5):49−56.]

    DU Liu, QIU Wenxing, LIU Dongyin, et al. Effects of different cooking methods on the physicochemical properties and flavor of crawfish (Procambarus clarkia)[J]. Meat Research, 2023, 37(5): 49−56.
    [12]
    付娜, 王锡昌. 电子舌分析和感官评价在游离氨基酸对中华绒螯蟹整体滋味贡献评价中的研究[J]. 食品工业科技,2014,35(20):91−96. [FU Na, WANG Xichang. Study on the contribution of free amino acid composition to the Chinese mitten crab taste by sensory evaluation and the electronic tongue[J]. Science and Technology of Food Industry,2014,35(20):91−96.]

    FU Na, WANG Xichang. Study on the contribution of free amino acid composition to the Chinese mitten crab taste by sensory evaluation and the electronic tongue[J]. Science and Technology of Food Industry, 2014, 35(20): 91−96.
    [13]
    KONG Y, ZHANG L L, SUN Y, et al. Determination of the free amino acid, organic acid, and nucleotide in commercial vinegars[J]. Journal of Food Science,2017,82(5):1116−1123. doi: 10.1111/1750-3841.13696
    [14]
    韩昕苑, 樊震宇, 从娇娇, 等. 冻融循环过程中冷冻罗非鱼片呈味物质的变化[J]. 食品科学,2022,43(2):269−275. [HAN Xinyuan, FAN Zhenyu, CONG Jiaojiao, et al. Changes of taste substances in frozen tilapia (Oreochromis niloticus) fillets during freeze-thaw cycles[J]. Food Science,2022,43(2):269−275.]

    HAN Xinyuan, FAN Zhenyu, CONG Jiaojiao, et al. Changes of taste substances in frozen tilapia (Oreochromis niloticus) fillets during freeze-thaw cycles[J]. Food Science, 2022, 43(2): 269−275.
    [15]
    SHAKER M. Comparison between traditional deep-fat frying and air-frying for production of healthy fried potato strips[J]. International Food Research Journal,2015,22(4):1557−1563.
    [16]
    金文刚, 赵萍, 姜鹏飞, 等. 基于GC-IMS技术结合多元统计模型分析不同色泽小米粥挥发性有机物差异[J]. 食品科学,2023,44(6):277−284. [JIN Wengang, ZHAO Ping, JIANG Pengfei, et al. Analysis of differential volatile organic compounds in different colored millet porridges by gas chromatography-ion mobility spectrometry combined with multivariate statistical analysis[J]. Food Science,2023,44(6):277−284.]

    JIN Wengang, ZHAO Ping, JIANG Pengfei, et al. Analysis of differential volatile organic compounds in different colored millet porridges by gas chromatography-ion mobility spectrometry combined with multivariate statistical analysis[J]. Food Science, 2023, 44(6): 277−284.
    [17]
    张权, 王为, 吴思纷, 等. 黑鱼油精制过程中品质及风味成分变化[J]. 食品科学, 2023, 44(12):208-216. [ZHANG Quan, WANG Wei, WU Sifen, et al. Changes in the quality and flavor components of snakehead fish oil during refining[J]. Food Science, 2023, 44(12):208-216.]

    ZHANG Quan, WANG Wei, WU Sifen, et al. Changes in the quality and flavor components of snakehead fish oil during refining[J]. Food Science, 2023, 44(12): 208-216.
    [18]
    乞萌, 魏冠棉, 李丽娜, 等. 不同酵母发酵面团特性分析及其对馒头品质的影响[J]. 食品科学,2024,45(12):59−67. [QI Meng, LI lina, XU Chao, et al. Characteristics of dough fermented by different yeasts and quality analysis of steamed bread[J]. Food Science,2024,45(12):59−67.]

    QI Meng, LI lina, XU Chao, et al. Characteristics of dough fermented by different yeasts and quality analysis of steamed bread[J]. Food Science, 2024, 45(12): 59−67.
    [19]
    倪瑞洁, 詹萍, 田洪磊. 基于GC-IMS结合多元统计方法分析炸制时间对花椒调味油挥发性物质的影响[J]. 食品科学, 2022, 43(6):279-86. [NI Ruijie, ZHAN Ping, TIAN Honglei. Effects of frying time on volatile flavor compounds in fried pepper (Zanthoxylum bungeanum) oil as analyzed by gas chromatography-ion mobility spectrometry and multivariate statistical analysis[J]. Food Science, 2022, 43(6):279-286.]

    NI Ruijie, ZHAN Ping, TIAN Honglei. Effects of frying time on volatile flavor compounds in fried pepper (Zanthoxylum bungeanum) oil as analyzed by gas chromatography-ion mobility spectrometry and multivariate statistical analysis[J]. Food Science, 2022, 43(6): 279-286.
    [20]
    关君兰, 姚雨萱, 伍菱, 等. 轻度盐腌大黄鱼的气味特征及形成途径[J]. 食品科学,2023,44(24):235−244. [GUAN Junlan, YAO Yuxuan, WU Ling, et al. Odor characteristics and formation pathways of low-salted large yellow croaker[J]. Food Science,2023,44(24):235−244.]

    GUAN Junlan, YAO Yuxuan, WU Ling, et al. Odor characteristics and formation pathways of low-salted large yellow croaker[J]. Food Science, 2023, 44(24): 235−244.
    [21]
    易宇文, 何莲, 邓静, 等. 基于电子鼻和气质联用识别不同工艺的俄色茶[J]. 食品工业科技,2023,44(18):361−370. [YI Yuwen, HE Lian, DENG Jing, et al. Identification of Malus toringoides (Rehd. ) Hughes teas with different processes based on E-nose and GC-MS[J]. Science and Technology of Food Industry,2023,44(18):361−370.]

    YI Yuwen, HE Lian, DENG Jing, et al. Identification of Malus toringoides (Rehd. ) Hughes teas with different processes based on E-nose and GC-MS[J]. Science and Technology of Food Industry, 2023, 44(18): 361−370.
    [22]
    刘同, 杨悠悠, 刘大鹏, 等. 肉鸭胸肌特异挥发性风味物质的鉴定[J]. 畜牧兽医学报,2022,53(2):402−413. [LIU Tong, YANG Youyou, LIU Dapeng, et al. Identification of specific volatile flavor compounds in breast muscle of meat duck[J]. Acta Veterinaria et Zootechnica Sinica,2022,53(2):402−413.]

    LIU Tong, YANG Youyou, LIU Dapeng, et al. Identification of specific volatile flavor compounds in breast muscle of meat duck[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 402−413.
    [23]
    CADWALLADER K R, TAN Q, CHEN F, et al. Evaluation of the aroma of cooked spiny lobster tail meat by aroma extract dilution analysis[J]. Journal of Agricultural and Food Chemistry,1995,43(9):2432−2437. doi: 10.1021/jf00057a022
    [24]
    BAINY E M, BERTAN L C, CORAZZA M L, et al. Effect of grilling and baking on physicochemical and textural properties of tilapia (Oreochromis niloticus) fish burger[J]. Journal of Food Science and Technology,2015,52:5111−5119. doi: 10.1007/s13197-014-1604-3
    [25]
    孙艺飞, 崔文甲, 王文亮, 等. 鸡脂和美拉德反应对松蘑菌汤风味的影响[J]. 食品工业科技,2022,43(19):98−107. [SUN Yifei, CUI Wenjia, WANG Wenliang, et al. Effect of chicken fat and Maillard reaction on flavor of tricholoma matsutake soup[J]. Science and Technology of Food Industry,2022,43(19):98−107.]

    SUN Yifei, CUI Wenjia, WANG Wenliang, et al. Effect of chicken fat and Maillard reaction on flavor of tricholoma matsutake soup[J]. Science and Technology of Food Industry, 2022, 43(19): 98−107.
    [26]
    鲁金花, 谢定, 鲜灵芝. 发酵型与浸泡型杨梅酒的挥发性成分分析[J]. 食品与机械,2022(6):31−36. [LU Jinhua, XIE Ding, XIAN Lingzhi. Analysis of volatile components of fermented and soaked bayberry wine[J]. Food & Machinery,2022(6):31−36.]

    LU Jinhua, XIE Ding, XIAN Lingzhi. Analysis of volatile components of fermented and soaked bayberry wine[J]. Food & Machinery, 2022(6): 31−36.
    [27]
    王浩文, 邓静, 唐红梅, 等. 不同品牌樟茶鸭风味特征分析[J]. 食品工业科技,2020,41(6):215−222,227. [WANG Haowen, DENG Jing, TANG Honmei, et al. Analysis of flavor characteristics of smoked duck from different brands[J]. Science and Technology of Food Industry,2020,41(6):215−222,227.]

    WANG Haowen, DENG Jing, TANG Honmei, et al. Analysis of flavor characteristics of smoked duck from different brands[J]. Science and Technology of Food Industry, 2020, 41(6): 215−222,227.
    [28]
    张权, 李金林, 胡明明, 等. 基于电子鼻和SAFE-GC-MS分析调味小龙虾挥发性风味特征差异[J/OL]. 食品与发酵工业:1−14. [2025-01-21]. https://doi.org/10.13995/j.cnki.11-1802/ts.036446. [ZHANG Quan, LI Jinlin, HU Mingming, et al Analysis of volatile flavor characteristics of seasoned crayfish based on electronic nose and SAFE-GC-MS[J/OL]. Food and Fermentation Industries: 1−14. [2025-01-21]. https://doi.org/10.13995/j.cnki.11-1802/ts.036446.]

    ZHANG Quan, LI Jinlin, HU Mingming, et al Analysis of volatile flavor characteristics of seasoned crayfish based on electronic nose and SAFE-GC-MS[J/OL]. Food and Fermentation Industries: 1−14. [2025-01-21]. https://doi.org/10.13995/j.cnki.11-1802/ts.036446.
    [29]
    PAVLIDIS D E, MALLOUCHOS A, ERCOLINI D, et al. A volatilomics approach for off-line discrimination of minced beef and pork meat and their admixture using HS-SPME GC/MS in tandem with multivariate data analysis[J]. Meat Science,2019,151:43−53. doi: 10.1016/j.meatsci.2019.01.003
    [30]
    江津津, 严静, 郑玉玺, 等. 不同产地传统鱼露风味特征差异分析[J]. 食品科学,2021,42(12):206−214. [JIANG Jinjin, YAN Jing, ZHENG Yuxi, et al. Analysis of flavor characteristics of traditional fish sauce from different regions[J]. Food Science,2021,42(12):206−214.]

    JIANG Jinjin, YAN Jing, ZHENG Yuxi, et al. Analysis of flavor characteristics of traditional fish sauce from different regions[J]. Food Science, 2021, 42(12): 206−214.
    [31]
    ZHANG W, ZHANG L, XU C. Chemical and volatile composition of jujube wines fermented by Saccharomyces cerevisiae with and without pulp contact and protease treatment[J]. Food Science and Technology,2016,36:204−209. doi: 10.1590/1678-457X.0011
    [32]
    余力, 贺稚非, 王兆明, 等. 不同解冻方式对伊拉兔肉挥发性风味物质的影响[J]. 食品科学,2015,36(22):95−101. [YU Li, HE Zhife, WANG Zhaoming, et al, et al. Effect of different thawing methods on volatile flavor compounds of hyla rabbit meat[J]. Food Science,2015,36(22):95−101.]

    YU Li, HE Zhife, WANG Zhaoming, et al, et al. Effect of different thawing methods on volatile flavor compounds of hyla rabbit meat[J]. Food Science, 2015, 36(22): 95−101.
    [33]
    黄慧清, 郑玉成, 胡清财, 等. 基于SBSE-GC-O-MS技术的3个代表性乌龙茶品种关键香气成分分析[J]. 食品科学,2024,45(1):101−108. [HUANG Huiqing, ZHENG Yucheng, HU Qingcai, et al. Analysis of key aroma components of three representative oolong tea varieties by stir bar sorptive extraction combined with gas chromatography-olfactory-mass spectrometry[J]. Food Science,2024,45(1):101−108.]

    HUANG Huiqing, ZHENG Yucheng, HU Qingcai, et al. Analysis of key aroma components of three representative oolong tea varieties by stir bar sorptive extraction combined with gas chromatography-olfactory-mass spectrometry[J]. Food Science, 2024, 45(1): 101−108.
    [34]
    ZHANG D, JI W, PENG Y, et al. Evaluation of flavor improvement in antarctic krill defluoridated hydrolysate by Maillard reaction using sensory analysis, E-nose, and GC-MS[J]. Journal of Aquatic Food Product Technology,2020,29(3):279−292. doi: 10.1080/10498850.2020.1723764
    [35]
    张曦鹏, 蒋中权, 郭全友, 等. 不同水分含量对轻腌大黄鱼贮藏期间细菌菌相和风味特征的影响[J]. 食品科学,2024,45(8):210−217. [ZHANG Xipeng, JIANG Zhongquan, GUO Quanyou, et al. Effects of different moisture content on bacterial flora and flavor characteristics of lightly salted large yellow croaker during storage[J]. Food Science,2024,45(8):210−217.]

    ZHANG Xipeng, JIANG Zhongquan, GUO Quanyou, et al. Effects of different moisture content on bacterial flora and flavor characteristics of lightly salted large yellow croaker during storage[J]. Food Science, 2024, 45(8): 210−217.
    [36]
    王蓓, 韩兆盛, 杨智杰, 等. 6类常见食品中含硫化合物风味特征及形成机理研究进展[J]. 食品科学技术学报, 2022, 40(6):13-25. [WANG Bei, HAN Zhaosheng, YANG Zhijie, et al. Research progress on flavor characteristics and formation mechanism of sulfur compounds in six common foods[J]Journal of Food Science and Technology, 2022, 40(6):13-25.]

    WANG Bei, HAN Zhaosheng, YANG Zhijie, et al. Research progress on flavor characteristics and formation mechanism of sulfur compounds in six common foods[J]Journal of Food Science and Technology, 2022, 40(6): 13-25.
    [37]
    ZHU Z S, FANG R, HUANG M, et al. Oxidation combined with Maillard reaction induced free and protein-bound Nε-carboxymethyllysine and Nε-carboxyethyllysine formation during braised chicken processing[J]. Food Science and Human Wellness,2020,9(4):383−393. doi: 10.1016/j.fshw.2020.05.013
    [38]
    刘振平, 龙道崎, 甘芳瑗, 等. 基于GC-IMS技术的油菜花蜂蜜产地溯源模型鉴别[J]. 中国食品学报,2023,23(8):379−388. [LIU Zhenping, LONG Daoqi, GAN Fangyuan, et al. Identification model for rape flower honey geographical origin based on GC-IMS technology[J]. Journal of Chinese Institute of Food Science and Technology,2023,23(8):379−388.]

    LIU Zhenping, LONG Daoqi, GAN Fangyuan, et al. Identification model for rape flower honey geographical origin based on GC-IMS technology[J]. Journal of Chinese Institute of Food Science and Technology, 2023, 23(8): 379−388.
    [39]
    吴倩蓉, 朱宁, 陈松, 等. 加工工艺对酱牛肉中蛋白质降解及风味物质的影响[J]. 食品科学,2021,42(12):76−84. [WU Qianrong, ZHU Ning, CHEN Song, et al. Changes in protein degradation and flavor substance in sauce beef during processing[J]. Food Science,2021,42(12):76−84.]

    WU Qianrong, ZHU Ning, CHEN Song, et al. Changes in protein degradation and flavor substance in sauce beef during processing[J]. Food Science, 2021, 42(12): 76−84.
    [40]
    岳宜静, 臧明伍, 刘海杰, 等. 干制南美白对虾贮藏过程中的肌原纤维蛋白氧化、滋味变化及其相关性分析[J]. 食品科学,2023,44(19):180−189. [YUE Yijing, ZANG Mingwu, LIU Haijie, et al. Changes and correlation between myofibrillar protein oxidation and taste of dried penaeus vannamei during storage[J]. Food Science,2023,44(19):180−189.]

    YUE Yijing, ZANG Mingwu, LIU Haijie, et al. Changes and correlation between myofibrillar protein oxidation and taste of dried penaeus vannamei during storage[J]. Food Science, 2023, 44(19): 180−189.
    [41]
    YAMAGUCHI S, YOSHIKAWA T, IKEDA S, et al. Measurement of the relative taste intensity of some l‐α‐amino acids and 5'‐nucleotides[J]. Journal of Food Science,1971,36(6):846−849. doi: 10.1111/j.1365-2621.1971.tb15541.x
    [42]
    张紫涵, 吴予灿, 宋玉, 等. 木瓜汁腌制对文昌鸡风味形成的影响[J]. 食品科学,2023,44(20):236−244. [ZHANG Zihan, WU Yucan, SONG Yu, et al. Effect of marination in papaya juice on the flavor formation of boiled wenchang chicken[J]. Food Science,2023,44(20):236−244.]

    ZHANG Zihan, WU Yucan, SONG Yu, et al. Effect of marination in papaya juice on the flavor formation of boiled wenchang chicken[J]. Food Science, 2023, 44(20): 236−244.
    [43]
    XIAO Z B, QU H L, MAO C T, et al. Study on the sweetening mechanism of aroma compounds in yangshan peach using sensory analysis, molecular docking, and molecular dynamics simulation techniques[J]. LWT,2024,191:115562. doi: 10.1016/j.lwt.2023.115562

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