Research Progress on the Bacteriostatic Mechanism, Biosynthesis and Application of Phenyllactic Acid

GUO Shuaikang; SONG Na; ZHANG Guo; LI Pei; LI Ku; CHEN Xiong

doi:10.13386/j.issn1002-0306.2024040383

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GUO Shuaikang, SONG Na, ZHANG Guo, et al. Research Progress on the Bacteriostatic Mechanism, Biosynthesis and Application of Phenyllactic Acid[J]. Science and Technology of Food Industry, 2025, 46(9): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040383.

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Research Progress on the Bacteriostatic Mechanism, Biosynthesis and Application of Phenyllactic Acid

1.
Collaborative Innovation Center for Industrial Fermentation Jointly Established by the Ministry of Education, Key Laboratory of Fermentation Engineering of the Ministry of Education, School of Life Science and Health Engineering of Hubei University of Technology, Wuhan 430068, China
2.
Angel Yeast Co., Ltd., Yichang 443000, China

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Received Date: April 27, 2024
Available Online: February 27, 2025

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Abstract

Abstract

Phenyllactic acid (PLA), produced by many lactic acid bacteria and non-lactic acid bacteria, is a natural substance with broad-spectrum antibacterial properties. As a green product with positive implications for food safety, it has received widespread attention. This work introduces the structure and physicochemical properties of PLA, elaborates on the latest progress in its antibacterial ability, bacteriostatic mechanism, biosynthesis and application. Factors that affect its yield and efficiency in the production process are also illustrated, which provide insights for industrial and market development.
- phenyllactic acid,
- physicochemical properties,
- antibacterial activity,
- biosynthesis,
- application

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[1]	WOODRUFF T J. Making it real—the environmental burden of disease. What does it take to make people pay attention to the environment and health?[J]. Journal of Clinical Endocrinology and Metabolism,2015,100(4):1241−1244. doi: 10.1210/jc.2015-1622
[2]	HUANG C H, CHEN W C, GAO Y H, et al. Production of phenyllactic acid from porphyra residues by lactic acid bacterial fermentation[J]. Processes,2021,9(4):678−688. doi: 10.3390/pr9040678
[3]	WU H, GUANG C, ZHANG W, et al. Recent development of phenyllactic acid:Physicochemical properties, biotechnological production strategies and applications[J]. Critical Reviews in Biotechnology,2023,43(2):293−308. doi: 10.1080/07388551.2021.2010645
[4]	GUAN J, HAN C, GUAN Y, et al. Optimizational production of phenyllactic acid by a Lactobacillus buchneri strain via uniform design with overlay sampling methodology[J]. Chinese Journal of Chemical Engineering,2019,27(2):418−425. doi: 10.1016/j.cjche.2018.04.005
[5]	陆永祥, 李平, 白史且. 苯乳酸的研究进展[J]. 草学,2020(4):19−24. [LU Y X, LI P, BAI S Q. Research progress on phenyllactic acid[J]. Cao Xue,2020(4):19−24.] LU Y X, LI P, BAI S Q. Research progress on phenyllactic acid[J]. Cao Xue, 2020（4）: 19−24.
[6]	吕孟敏, 白凤翎, 崔方超, 等. 鱼肠道中高产苯乳酸乳酸菌的筛选、鉴定及发酵条件优化[J]. 中国酿造,2023,42(9):125−130. [LÜ M M, BAI F L, CUI F C, et al. Screening, identification, and optimization of fermentation conditions for high-yield phenyllactic acid bacteria in fish intestines[J]. Chinese Brewing,2023,42(9):125−130.] LÜ M M, BAI F L, CUI F C, et al. Screening, identification, and optimization of fermentation conditions for high-yield phenyllactic acid bacteria in fish intestines[J]. Chinese Brewing, 2023, 42（9）: 125−130.
[7]	LUO X, WANG Y, ZHENG W, et al. Simultaneous improvement of the thermostability and activity of lactic dehydrogenase from Lactobacillus rossiae through rational design[J]. RSC Adv,2022,12(51):33251. doi: 10.1039/D2RA05599F
[8]	LI T, QIN Z, WANG D, et al. Coenzyme self-sufficiency system-recent advances in microbial production of high-value chemical phenyllactic acid[J]. World J Microbiol Biotechnol,2022,39(1):36−46.
[9]	HOU Y, GAO B, CUI J, et al. Combination of multi-enzyme expression fine-tuning and co-substrates addition improves phenyllactic acid production with an Escherichia coli whole-cell biocatalyst[J]. Bioresource Technology,2019,287:121423. doi: 10.1016/j.biortech.2019.121423
[10]	程一凡, 刘念丽, 侯颖. 苯乳酸的研究进展[J]. 发酵科技通讯,2023,52(4):212−217. [CHENG Y F, LIU N L, HOU Y. Research progress on phenyllactic acid[J]. Fermentation Technology Communication,2023,52(4):212−217.] CHENG Y F, LIU N L, HOU Y. Research progress on phenyllactic acid[J]. Fermentation Technology Communication, 2023, 52（4）: 212−217.
[11]	RAJANIKAR R V, NATARAJ B H, NAITHANI H, et al. Phenyllactic acid:A green compound for food biopreservation[J]. Food Control,2021,128:108184. doi: 10.1016/j.foodcont.2021.108184
[12]	NAVARE P S, MACDONALD J C. Investigation of stability and structure in three homochiral and heterochiral crystalline forms of 3-phenyllactic acid[J]. Crystal Growth & Design,2011,11(6):2422−2428.
[13]	CORTES-ZAVALETA O, LOPEZ-MALO A, HERNANDEZ-MENDOZA A, et al. Antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production[J]. Int J Food Microbiol,2014,173:30−35. doi: 10.1016/j.ijfoodmicro.2013.12.016
[14]	DIEULEVEUX V, VAN DER PYL D, CHATAUD J, et al. Purification and characterization of anti-listeria compounds produced by Geotrichum candidum[J]. Appl Environ Microbiol,1998,64(2):800−803. doi: 10.1128/AEM.64.2.800-803.1998
[15]	MAIONE A, IMPARATO M, BUONANNO A, et al. Anti-biofilm activity of phenyllactic acid against clinical isolates of fluconazole-resistant Candida albicans[J]. J Fungi (Basel),2023,9(3):335−351. doi: 10.3390/jof9030335
[16]	LI M, YAO B, MENG X. Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination[J]. J Basic Microbiol,2022,62(12):1457−1466. doi: 10.1002/jobm.202200274
[17]	FAN W, LI B, DU N, et al. Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae[J]. Int J Food Microbiol,2022,369:109606. doi: 10.1016/j.ijfoodmicro.2022.109606
[18]	NING Y, YAN A, YANG K, et al. Antibacterial activity of phenyllactic acid against Listeria monocytogenes and Escherichia coli by dual mechanisms[J]. Food Chemistry,2017,228:533−540. doi: 10.1016/j.foodchem.2017.01.112
[19]	LIU F, WANG F, DU L, et al. Antibacterial and antibiofilm activity of phenyllactic acid against Enterobacter cloacae[J]. Food Control,2018,84:442−448. doi: 10.1016/j.foodcont.2017.09.004
[20]	JIANG Y H, YING J P, XIN W G, et al. Antibacterial activity and action target of phenyllactic acid against Staphylococcus aureus and its application in skim milk and cheese[J]. Journal of Dairy Science,2022,105(12):9463−9475. doi: 10.3168/jds.2022-22262
[21]	FANG M, WANG R, AGYEKUMWAA A K, et al. Antibacterial effect of phenyllactic acid against Vibrio parahaemolyticus and its application on raw salmon fillets[J]. LWT,2022,154:112586. doi: 10.1016/j.lwt.2021.112586
[22]	CHATTERJEE M, D'MORRIS S, PAUL V, et al. Mechanistic understanding of phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa[J]. Appl Microbiol Biotechnol,2017,101(22):8223−8236. doi: 10.1007/s00253-017-8546-4
[23]	JIANG Y H, YANG L Y, XIN W G, et al. Combined antibacterial and antibiofilm activity of phenyllactic acid and bacteriocin XJS01 against Shigella flexneri[J]. Food Bioscience,2022,45:101512. doi: 10.1016/j.fbio.2021.101512
[24]	ZHOU Q, GU R, LI P, et al. Anti-Salmonella mode of action of natural L-phenyl lactic acid purified from Lactobacillus plantarum ZJ316[J]. Appl Microbiol Biotechnol,2020,104(12):5283−5292. doi: 10.1007/s00253-020-10503-4
[25]	胡虹, 张秀江, 向凌云, 等. 高产D-苯乳酸菌株的选育及发酵工艺的试验研究[J]. 饲料研究,2021,44(22):73−77. [HU H, ZHANG X J, XIANG L Y, et al. Experimental study on the breeding and fermentation process of high-yield D-phenyllactic acid strains[J]. Feed Research,2021,44(22):73−77.] HU H, ZHANG X J, XIANG L Y, et al. Experimental study on the breeding and fermentation process of high-yield D-phenyllactic acid strains[J]. Feed Research, 2021, 44（22）: 73−77.
[26]	LUO X, ZHANG Y, YIN L, et al. Efficient synthesis of d-phenyllactic acid by a whole-cell biocatalyst co-expressing glucose dehydrogenase and a novel d-lactate dehydrogenase from Lactobacillus rossiae[J]. 3 Biotech,2020,10(1):1−9. doi: 10.3390/biotech10010001
[27]	LUO X, ZHANG Y, YIN F, et al. Enzymological characterization of a novel d-lactate dehydrogenase from Lactobacillus rossiae and its application in d-phenyllactic acid synthesis[J]. 3 Biotech,2020,10(3):1−2.
[28]	DIEULEVEUX V, LEMARINIER S, GUEGUEN M. Antimicrobial spectrum and target site of D-3-phenyllactic acid[J]. Int J Food Microbiol,1998,40(3):177−183. doi: 10.1016/S0168-1605(98)00031-2
[29]	SVANSTROM A, BOVERI S, BOSTROM E, et al. The lactic acid bacteria metabolite phenyllactic acid inhibits both radial growth and sporulation of filamentous fungi[J]. BMC Res Notes,2013,6(1):1−9. doi: 10.1186/1756-0500-6-1
[30]	SORRENTINO E, TREMONTE P, SUCCI M, et al. Detection of antilisterial activity of 3-phenyllactic acid using Listeria innocua as a model[J]. Front Microbiol,2018,9:1373−1381. doi: 10.3389/fmicb.2018.01373
[31]	SHAKYA S, DANSHIITSOODOL N, NODA M, et al. 3-Phenyllactic acid generated in medicinal plant extracts fermented with plant-derived lactic acid bacteria inhibits the biofilm synthesis of Aggregatibacter actinomycetemcomitans[J]. Front Microbiol,2022,13:991144. doi: 10.3389/fmicb.2022.991144
[32]	ZHAO C, PENTTINEN P, ZHANG L, et al. Mechanism of inhibiting the growth and aflatoxin B(1) biosynthesis of Aspergillus flavus by phenyllactic acid[J]. Toxins (Basel),2023,15(6):370−385. doi: 10.3390/toxins15060370
[33]	SHARIFF M, CHATTERJEE M, MORRIS S D, et al. Enhanced inhibition of Pseudomonas aeruginosa virulence factor production and biofilm development by sublethal concentrations of eugenol and phenyllactic acid[J]. Lett Appl Microbiol,2022,75(5):1336−1345. doi: 10.1111/lam.13803
[34]	REUTER K, STEINBACH A, HELMS V. Interfering with bacterial quorum sensing[J]. Perspect Medicin Chem,2016,8:1−15.
[35]	宁亚维, 付浴男, 何建卓, 等. 苯乳酸和醋酸联用对单核细胞增生李斯特菌的协同抑菌机理[J]. 食品科学,2020,41(23):70−76. [NING Y W, FU Y N, HE J Z, et al. Synergistic antibacterial mechanism of the combination of phenyllactic acid and acetic acid against Listeria monocytogenes[J]. Food Science,2020,41(23):70−76.] NING Y W, FU Y N, HE J Z, et al. Synergistic antibacterial mechanism of the combination of phenyllactic acid and acetic acid against Listeria monocytogenes[J]. Food Science, 2020, 41（23）: 70−76.
[36]	WANG R, HU X, AGYEKUMWAA A K, et al. Synergistic effect of kojic acid and tea polyphenols on bacterial inhibition and quality maintenance of refrigerated sea bass (Lateolabrax japonicus) fillets[J]. LWT,2021,137:110452. doi: 10.1016/j.lwt.2020.110452
[37]	NING Y, FU Y, HOU L, et al. iTRAQ-based quantitative proteomic analysis of synergistic antibacterial mechanism of phenyllactic acid and lactic acid against Bacillus cereus[J]. Food Res Int,2021,139:109562. doi: 10.1016/j.foodres.2020.109562
[38]	闫爱红. 苯乳酸与常用食品防腐剂和有机酸的联合抑菌作用及机理研究[D]. 石家庄:河北科技大学, 2016. [YAN A H. Study on the combined antibacterial effect and mechanism of phenyllactic acid with commonly used food preservatives and organic acids[D]. Shijiazhuang:Hebei University of Science and Technology, 2016.] YAN A H. Study on the combined antibacterial effect and mechanism of phenyllactic acid with commonly used food preservatives and organic acids[D]. Shijiazhuang: Hebei University of Science and Technology, 2016.
[39]	LIU J, HUANG R, SONG Q, et al. Combinational antibacterial activity of nisin and 3-phenyllactic acid and their co-production by engineered Lactococcus lactis[J]. Frontiers in Bioengineering and Biotechnology,2021,9:612105. doi: 10.3389/fbioe.2021.612105
[40]	YU S, ZHOU C, ZHANG T, et al. 3-Phenyllactic acid production in milk by SK25 during laboratory fermentation process[J]. Journal of Dairy Science,2015,98(2):813−817. doi: 10.3168/jds.2014-8645
[41]	XU J J, SUN J Z, SI K L, et al. 3-Phenyllactic acid production by Lactobacillus crustorum strains isolated from naturally fermented vegetables[J]. LWT,2021,149:111780. doi: 10.1016/j.lwt.2021.111780
[42]	赵天行. 产苯乳酸乳酸菌的生物学特性分析及其在食醋生产中的应用[D]. 武汉:华中农业大学, 2022. [ZHAO T X. Biological characteristics analysis of lactic acid bacteria producing phenyllactic acid and their application in vinegar production[D]. Wuhan:Huazhong Agricultural University, 2022.] ZHAO T X. Biological characteristics analysis of lactic acid bacteria producing phenyllactic acid and their application in vinegar production[D]. Wuhan: Huazhong Agricultural University, 2022.
[43]	KAWTHARANI H, SNINI S P, HEANG S, et al. Phenyllactic acid produced by Geotrichum candidum reduces Fusarium sporotrichioides and F. langsethiae growth and T-2 toxin concentration[J]. Toxins (Basel),2020,12(4):209−224. doi: 10.3390/toxins12040209
[44]	魏娜. 猪源乳酸菌分离鉴定及初步应用[D]. 成都:四川农业大学, 2016. [WEI N. Isolation, identification and preliminary application of lactic acid bacteria from pigs[D]. Chengdu:Sichuan Agricultural University, 2016.] WEI N. Isolation, identification and preliminary application of lactic acid bacteria from pigs[D]. Chengdu: Sichuan Agricultural University, 2016.
[45]	李士龙. 传统发酵食品中高产苯乳酸菌株的筛选及发酵工艺条件优化[D]. 大庆:黑龙江八一农垦大学, 2012. [LI S L. Screening of high-yield strains of phenyllactic acid in traditional fermented foods and optimization of fermentation process conditions[D]. Daqing:Heilongjiang Bayi Agricultural University, 2012.] LI S L. Screening of high-yield strains of phenyllactic acid in traditional fermented foods and optimization of fermentation process conditions[D]. Daqing: Heilongjiang Bayi Agricultural University, 2012.
[46]	吴文妤. 植物乳杆菌YM-4-3苯乳酸合成代谢调控机制及相关基因的功能研究[D]. 昆明:昆明理工大学, 2021. [WU W Y. Research on the regulatory mechanism of phenyllactic acid synthesis metabolism and related genes in Lactobacillus plantarum YM-4-3[D]. Kunming:Kunming University of Science and Technology, 2021.] WU W Y. Research on the regulatory mechanism of phenyllactic acid synthesis metabolism and related genes in Lactobacillus plantarum YM-4-3[D]. Kunming: Kunming University of Science and Technology, 2021.
[47]	倪正, 关今韬, 沈绍传, 等. 苯乳酸的微生物合成及分离研究进展[J]. 化工进展,2016,35(11):3627−3633. [NI Z, GUAN J T, SHEN S C, et al. An overview of recent advances in microbial synthesis and separation of phenyllactic acid[J]. Chemical Industry and Engineering Progress,2016,35(11):3627−3633.] NI Z, GUAN J T, SHEN S C, et al. An overview of recent advances in microbial synthesis and separation of phenyllactic acid[J]. Chemical Industry and Engineering Progress, 2016, 35（11）: 3627−3633.
[48]	WU W, DENG G, LIU C, et al. Optimization and multiomic basis of phenyllactic acid overproduction by Lactobacillus plantarum[J]. J Agric Food Chem,2020,68(6):1741−1749. doi: 10.1021/acs.jafc.9b07136
[49]	KAWAGUCHI H, MIYAGAWA H, NAKAMURA-TSURUTA S, et al. Enhanced phenyllactic acid production in Escherichia coli via oxygen limitation and shikimate pathway gene expression[J]. Biotechnology Journal,2019,14(6):10.
[50]	YOO J A, LIM Y M, YOON M H. Production and antifungal effect of 3-phenyllactic acid (PLA) by lactic acid bacteria[J]. Journal of Applied Biological Chemistry,2016,59(3):173−178. doi: 10.3839/jabc.2016.032
[51]	VERMEULEN N, GÁNZLE M G, VOGEL R F. Influence of peptide supply and cosubstrates on phenylalanine metabolism of Lactobacillus sanfranciscensis DSM20451T and Lactobacillus plantarum TMW1.468[J]. Journal of Agricultural and Food Chemistry,2006,54(11):3832−3829. doi: 10.1021/jf052733e
[52]	ZHANG D, ZHANG T, LEI Y, et al. Enantioselective biosynthesis of L-phenyllactic acid from phenylpyruvic acid in vitro by L-lactate dehydrogenase coupling with glucose dehydrogenase[J]. Front Bioeng Biotechnol,2022,10:846489. doi: 10.3389/fbioe.2022.846489
[53]	田雪娇. 高产苯乳酸菌株的诱变选育及发酵条件优化[D]. 大庆:黑龙江八一农垦大学. 2013. [TIAN X J. Breeding of high-yield phenyllactic acid strains and optimization of fermentation conditions[D]. Daqing:Heilongjiang Bayi Agricultural University, 2013.] TIAN X J. Breeding of high-yield phenyllactic acid strains and optimization of fermentation conditions[D]. Daqing: Heilongjiang Bayi Agricultural University, 2013.
[54]	ZHOU Y, SUN X, HU J, et al. Enhanced catalytic activity and stability of lactate dehydrogenase for cascade catalysis of D-PLA by rational design[J]. J Biotechnol,2024,382:1−7. doi: 10.1016/j.jbiotec.2024.01.004
[55]	HOU Y, HOSSAIN G S, LI J, et al. Production of phenylpyruvic acid from L-phenylalanine using an L-amino acid deaminase from Proteus mirabilis:Comparison of enzymatic and whole-cell biotransformation approaches[J]. Appl Microbiol Biotechnol,2015,99(20):8391−402. doi: 10.1007/s00253-015-6757-0
[56]	REEVE H A, LAUTERBACH L, LENZ O, et al. Enzyme-modified particles for selective biocatalytic hydrogenation by hydrogen-driven NADH recycling[J]. ChemCatChem,2015,7(21):3480−3487. doi: 10.1002/cctc.201500766
[57]	QIN Z, WANG D, LUO R, et al. Using unnatural protein fusions to engineer a coenzyme self-sufficiency system for D-phenyllactic acid biosynthesis in Escherichia coli[J]. Front Bioeng Biotechnol,2021,9:795885. doi: 10.3389/fbioe.2021.795885
[58]	ROCHA R A, NORTH A J, SPEIGHT R E, et al. Cofactor and process engineering for nicotinamide recycling and retention in intensified biocatalysis[J]. Catalysts,2022,12(11):1454−1466. doi: 10.3390/catal12111454
[59]	晏雄鹰, 王振, 娄吉芸, 等. 生物燃料高效生产微生物细胞工厂构建研究进展[J]. 合成生物学, 2023, 4(6):1082-121. [YAN X Y, WANG Z, LOU J Y, et al. Research progress on the construction of microbial cell factories for efficient production of biofuels[J]. Synthetic Biology, 2023, 4(6):1082.] YAN X Y, WANG Z, LOU J Y, et al. Research progress on the construction of microbial cell factories for efficient production of biofuels[J]. Synthetic Biology, 2023, 4（6）: 1082.
[60]	ZHOU X, ZHOU J, XIN F, et al. Heterologous expression of a novel d-lactate dehydrogenase from Lactobacillus sp. ZX1 and its application for d-phenyllactic acid production[J]. International Journal of Biological Macromolecules,2018,119:1171−1178. doi: 10.1016/j.ijbiomac.2018.08.036
[61]	ZHU Y, JIANG Z, CHEN J, et al. Fusion of D-lactate dehydrogenase and formate dehydrogenase for increasing production of (R)-3-phenyllactic acid in recombinant Escherichia coli BL21 (DE3)[J]. Journal of Biobased Materials and Bioenergy,2017,11(4):372−378. doi: 10.1166/jbmb.2017.1680
[62]	WANG X, HOU Y, LIU L, et al. A new approach for efficient synthesis of phenyllactic acid from L-phenylalanine:Pathway design and cofactor engineering[J]. Journal of Food Biochemistry,2018,42(5):e12584. doi: 10.1111/jfbc.12584
[63]	XU H, CHENG Q, QIU Y, et al. A novel strategy for whole-cell biotransformation enabling simultaneous L-phenyllactic acid production and coenzyme regeneration[J]. Journal of Agricultural and Food Chemistry,2023,71(51):20772−20781. doi: 10.1021/acs.jafc.3c06387
[64]	LOU X, JIANG Y, ZHAO F, et al. Preparation and characterization of semi-hydrophobic cryogels for culture of Lactobacillus strains and bioconversion towards phenyllactic acid bioproduction[J]. Biochemical Engineering Journal,2022,179:108312. doi: 10.1016/j.bej.2021.108312
[65]	SUN X, HU J, WANG Y, et al. One-pot encapsulation of lactate dehydrogenase and Fe₃O₄ nanoparticles into a metal-organic framework:A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid[J]. Front Bioeng Biotechnol,2022,10:1124450.
[66]	CHEN H, YAN X, DU G, et al. Recent developments in antifungal lactic acid bacteria:Application, screening methods, separation, purification of antifungal compounds and antifungal mechanisms[J]. Critical Reviews in Food Science and Nutrition,2021,63(15):2544−2558.
[67]	林广智. 水果保鲜纸基材料的结构与性能研究[D]. 济南:齐鲁工业大学, 2023. [LIN G Z. Research on the structure and properties of fruit preservation paper based materials[D]. Jinan:Qilu University of Technology, 2023.] LIN G Z. Research on the structure and properties of fruit preservation paper based materials[D]. Jinan: Qilu University of Technology, 2023.
[68]	GAO Y, LI Y, LI F, et al. Phenyllactic acid treatment for controlling anthracnose disease (Colletotrichum musae) and preserving banana fruit quality during storage[J]. Physiological and Molecular Plant Pathology,2024,129:102181. doi: 10.1016/j.pmpp.2023.102181
[69]	WAN Y L , CHEN L S, XIE D, et al. The effect of D-phenyllactic acid combined with coating treatment on anti-microbial and preservation of grapes during storage[J]. Food and Machinery, 2018, 34(1):117-120.
[70]	高羽莎, 李奕星, 李芬芳, 等. 苯乳酸处理对香蕉果实后熟品质的影响[J]. 保鲜与加工,2024,24(2):8−14. [GAO Y S, LI Y X, LI F F, et al. The effect of phenyllactic acid treatment on the ripening quality of banana fruits[J]. Preservation and Processing,2024,24(2):8−14.] doi: 10.3969/j.issn.1009-6221.2024.02.002 GAO Y S, LI Y X, LI F F, et al. The effect of phenyllactic acid treatment on the ripening quality of banana fruits[J]. Preservation and Processing, 2024, 24（2）: 8−14. doi: 10.3969/j.issn.1009-6221.2024.02.002
[71]	XU H, LIU J, JIN Y, et al. Phenyllactic acid maintains the postharvest quality of winter jujube fruit via activating ascorbic acid metabolism[J]. Scientia Horticulturae,2023,322:112443. doi: 10.1016/j.scienta.2023.112443
[72]	ZHANG S, LI C, WANG M, et al. Phenyllactic acid maintains the storage quality of ‘Zaosu’ pears by regulating respiration and energy metabolism[J]. Postharvest Biology and Technology,2024,207:112607. doi: 10.1016/j.postharvbio.2023.112607
[73]	HUANG P, YU L, TIAN F, et al. Untargeted metabolomics revealed the key metabolites in milk fermented with starter cultures containing Lactobacillus plantarum CCFM8610[J]. LWT,2022,165:113768. doi: 10.1016/j.lwt.2022.113768
[74]	ZHANG D, TONG D, WANG Z, et al. Inactivation mechanism of phenyllactic acid against Bacillus cereus spores and its application in milk beverage[J]. Food Chemistry,2024,453:139601. doi: 10.1016/j.foodchem.2024.139601
[75]	JIANG X, JIANG C, YU T, et al. Phenyllactic acid application to control Listeria monocytogenes biofilms and its growth in milk and spiced beef[J]. Int J Food Microbiol,2022,381:109910. doi: 10.1016/j.ijfoodmicro.2022.109910
[76]	VALERIO F. Production of phenyllactic acid by lactic acid bacteria:An approach to the selection of strains contributing to food quality and preservation[J]. FEMS Microbiology Letters,2004,233(2):289−295. doi: 10.1111/j.1574-6968.2004.tb09494.x
[77]	YVON M, RIJNEN L. Cheese flavour formation by amino acid catabolism[J]. International Dairy Journal,2001,11(4−7):185−201. doi: 10.1016/S0958-6946(01)00049-8
[78]	KIERONCZYK A, SKEIE S, LANGSRUD T, et al. Cooperation between Lactococcus lactis and nonstarter Lactobacilli in the formation of cheese aroma from amino acids[J]. Applied and Environmental Microbiology,2003,69(2):734−739. doi: 10.1128/AEM.69.2.734-739.2003
[79]	ZHOU Q, GU R, XUE B, et al. Phenyl lactic acid alleviates Samonella Typhimurium-induced colitis via regulating microbiota composition, SCFA production and inflammatory responses[J]. Food & Function,2021,12(12):5591−5606.
[80]	路飞, 顾孟清, 朱轶群, 等. 天然保鲜剂的抗菌机理及在肉制品中的应用[J]. 沈阳师范大学学报(自然科学版),2023,41(5):419−426. [LU F, GU M Q, ZHU Y Q. et al. The antibacterial mechanism of natural preservatives and their application in meat products[J]. Journal of Shenyang Normal University (Natural Science Edition),2023,41(5):419−426.] LU F, GU M Q, ZHU Y Q. et al. The antibacterial mechanism of natural preservatives and their application in meat products[J]. Journal of Shenyang Normal University （Natural Science Edition）, 2023, 41（5）: 419−426.
[81]	ZHENG R, ZHAO T, HUNG Y C, et al. Evaluation of bactericidal effects of phenyllactic acid on Escherichia coli O157:H7 and Salmonella Typhimurium on beef meat[J]. Journal of Food Protection,2019,82(12):2016−2022. doi: 10.4315/0362-028X.JFP-19-217
[82]	刘绍鹏, 曹秀娟, 贺峰. 苯乳酸复合保鲜剂在鸡肉保鲜中的应用[J]. 肉类工业,2019(6):46−50. [LIU S P, CAO X J, HE F. Application of phenyllactic acid compound preservative in chicken preservation[J]. Meat Industry,2019(6):46−50.] doi: 10.3969/j.issn.1008-5467.2019.06.010 LIU S P, CAO X J, HE F. Application of phenyllactic acid compound preservative in chicken preservation[J]. Meat Industry, 2019（6）: 46−50. doi: 10.3969/j.issn.1008-5467.2019.06.010
[83]	PIUS BASSEY A, PEI LIU P, CHEN J, et al. Antibacterial efficacy of phenyllactic acid against Pseudomonas lundensis and Brochothrix thermosphacta and its synergistic application on modified atmosphere/air-packaged fresh pork loins[J]. Food Chemistry,2024,430:137002. doi: 10.1016/j.foodchem.2023.137002
[84]	CAMPOS C A, AUBOURG S P, SCHELEGUEDA L I. Use of biopreservation to improve the quality of fresh aquatic products [M]. Postharvest and Postmortem Processing of Raw Food Materials, Elsevier, 2022:343-378.
[85]	黄琪, 王世哲, 胡传峰, 等. 苯乳酸处理对鮰鱼低温贮藏品质的影响[J]. 肉类研究,2023,37(11):35−41. [HUANG Q, WANG S Z, HU C F, et al. The effect of phenyllactic acid treatment on the low-temperature storage quality of catfish[J]. Meat Research,2023,37(11):35−41.] HUANG Q, WANG S Z, HU C F, et al. The effect of phenyllactic acid treatment on the low-temperature storage quality of catfish[J]. Meat Research, 2023, 37（11）: 35−41.
[86]	范晓然. 太湖白虾低温贮藏时的菌相分析及其保鲜剂的开发[D]. 南京:南京财经大学, 2018. [FAN X R. Microflora analysis and preservation of Taihu white prawn (Exopalaemon modestus) during low temperature storage[D]. Nanjing:Nanjing University of Finance Economics, 2018.] FAN X R. Microflora analysis and preservation of Taihu white prawn （Exopalaemon modestus） during low temperature storage[D]. Nanjing: Nanjing University of Finance Economics, 2018.
[87]	MANU D K. Antimicrobial effectiveness of phenyllactic acid against foodborne pathogenicbacteria and Penicillium and Aspergillus molds[D]. ISU:University of Iowa, 2012.
[88]	DOPAZO V, ILLUECA F, LUZ C, et al. Evaluation of shelf life and technological properties of bread elaborated with lactic acid bacteria fermented whey as a bio-preservation ingredient[J]. LWT,2023,174:114427. doi: 10.1016/j.lwt.2023.114427
[89]	REN H, DU N, NIU X, et al. Inhibitory effects of L-3-phenyllacitc acid on the activity of mushnroom pholyphenol oxidase [J]. Food Science and Technology, 2021, 41(suppl 1):343-351.
[90]	孔保华, 刁新平. 冷却肉包装保鲜技术的研究进展[J]. 肉类研究,2008(2):54−59. [KONG B H, DIAO X P. The review of extending the shelf-life of chilled meat by packaging[J]. Meat Research,2008(2):54−59.] doi: 10.3969/j.issn.1001-8123.2008.02.015 KONG B H, DIAO X P. The review of extending the shelf-life of chilled meat by packaging[J]. Meat Research, 2008（2）: 54−59. doi: 10.3969/j.issn.1001-8123.2008.02.015
[91]	LIU Y, WANG R, WANG D, et al. Development of a food packaging antibacterial hydrogel based on gelatin, chitosan, and 3-phenyllactic acid for the shelf-life extension of chilled chicken[J]. Food Hydrocolloids, 2022, 127.
[92]	ZHANG J, CHEN J, ZHANG C, et al. Characterization and antibacterial properties of chitosan–polyvinyl alcohol-3-phenyllactic acid as a biodegradable active food packaging[J]. Food Packaging and Shelf Life, 2022, 34.
[93]	SUN X, YIN L, ZHU H, et al. Enhanced antimicrobial cellulose/chitosan/ZnO biodegradable composite membrane[J]. Membranes (Basel),2022,12(2):239−251. doi: 10.3390/membranes12020239
[94]	李兴峰, 江波, 潘蓓蕾. 新型生物防腐剂——苯乳酸在食品中的研究与应用[J]. 食品与发酵工业,2007(5):87−91. [LI X F, JIANG B, PAN B L. Research and application in food of phenyllactic acid as a novel biopreservative:A review[J]. Food and Fermentation Industries,2007(5):87−91.] doi: 10.3321/j.issn:0253-990X.2007.05.022 LI X F, JIANG B, PAN B L. Research and application in food of phenyllactic acid as a novel biopreservative: A review[J]. Food and Fermentation Industries, 2007（5）: 87−91. doi: 10.3321/j.issn:0253-990X.2007.05.022