Screening of Postbiotics against <i>Salmonella</i> and Whole Genome Analysis of the Original Strain

HUANG Yan; SU Yue; LI Shiyang; QI Xuehe; JIA Ai; JIANG Yujun; YANG Xinyan; MAN Chaoxin

doi:10.13386/j.issn1002-0306.2024040240

Volume 46 Issue 6

Mar. 2025

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Science and Technology of Food Industry > 2025 > 46(6): 195-205. > DOI: 10.13386/j.issn1002-0306.2024040240

HUANG Yan, SU Yue, LI Shiyang, et al. Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain[J]. Science and Technology of Food Industry, 2025, 46(6): 195−205. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040240.

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Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Heilongjiang Institute of Quality Supervision and Testing, Harbin 150030, China

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Received Date: April 21, 2024
Available Online: January 07, 2025

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Abstract

Abstract

Objective: To screen postbiotics with exceptional anti-Salmonella activity and develop postbiotic raw materials with industrialization value. Methods: The anti-Salmonella potential of 30 Lactobacillus postbiotic strains was evaluated, focusing on their ability to inhibit the growth of Salmonella enterica serovar Typhimurium (ST). The top five most effective strains were identified, and further assays were conducted to assess their capacity to inhibit ST adhesion and invasion in HT-29 cells. The most significant effective postbiotic was selected for whole genome sequencing and safety evaluation of its originating strain. Results: Lactobacillus plantarum (L. plantarum) JM015 postbiotic exhibited a notable inhibition zone diameter of 15.12±0.53 mm. It effectively reduced the adhesion and invasion of Salmonella to HT-29 cells by more than 90%. Whole genome sequencing of L. plantarum JM015 revealed four bacteriostasis-related gene clusters related to repression: cyclic-lactone-autoinducer, terpene, ribosomally synthesized and post-translationally modified peptide (RiPP)-like, and type III polyketide synthases (PKS). Additionally, it harbors a gene cluster associated with plantaricin production and several genes linked to adhesion, such as fibronectin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and triosephosphate isomerase. The safety of the original strain was confirmed through tests for indole production, hemolysis, and nitrate reductase activity. Conclusion: The L. plantarum JM015 postbiotic demonstrates potent anti-Salmonella activity and can as a novel postbiotic ingredient for product development.
- Lactobacillus,
- postbiotic,
- Salmonella enterica serovar Typhimurium,
- antibacterial,
- whole-genome,
- safety evaluation

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1.	涂玲飞，陈迎丽，李焱，张振. 白及多糖锌的结构表征及其抗氧化活性评价. 食品与发酵工业. 2024(17): 218-225+245 .
2.	宋林梦，孔烁，蔡雨情，高慧，余远，纪雪莹，陶飞燕，薛鹏. 基于响应面法优化弱酸热催化制备藜麦低极性皂苷及活性研究. 食品科技. 2023(09): 192-200 .