Optimization of Extraction Process of <i>Isaria cicadae</i> Miquel Spore Oil and Effect on Inflammation and Oxidative Damage of Intestinal Epithelial Cells

LI Xingyang; WANG Beiqi; GUO Chao; CAO Hui; YU Ruilian; WANG Qin

doi:10.13386/j.issn1002-0306.2024040177

Volume 46 Issue 5

Feb. 2025

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Science and Technology of Food Industry > 2025 > 46(5): 187-195. > DOI: 10.13386/j.issn1002-0306.2024040177

LI Xingyang, WANG Beiqi, GUO Chao, et al. Optimization of Extraction Process of Isaria cicadae Miquel Spore Oil and Effect on Inflammation and Oxidative Damage of Intestinal Epithelial Cells[J]. Science and Technology of Food Industry, 2025, 46(5): 187−195. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040177.

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Optimization of Extraction Process of Isaria cicadae Miquel Spore Oil and Effect on Inflammation and Oxidative Damage of Intestinal Epithelial Cells

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210000, China
2.
Wuxi Institute of Inspection, Testing and Certification, Wuxi 214101, China

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Received Date: April 11, 2024
Available Online: January 01, 2025

Graphical Abstract

Abstract

Abstract

This study utilized solvent extraction to continuously reflux extract the lipid components of cultural Isaria cicadae Miquel spore powder, optimized the extraction process to maximize the yield of Isaria cicadae Miquel spores oil based on the single-factor experiments, and response surface analysis, explored its composition, anti-inflammatory activity in vitro and potential effects on repairing oxidative damage of intestinal epithelial cells. Results indicated that under the conditions of a liquid-solid ratio of 12:1 and a temperature of 55 ℃, the highest yield of Isaria cicadae Miquel spore oil was obtained after continuous countercurrent extraction for 3 hours, reaching 2.626%±0.04%. Additionally, 25 compounds were identified by gas chromatography-mass spectrometry (GC-MS) analysis, including 18 fatty acids, accounting for 87.86%. In vitro experiments demonstrated that Isaria cicadae Miquel spore oil at concentrations of 20~200 μg/mL, significantly increased RAW264.7 cell survival rate and significantly promoted cellular NO secretion to normal physiological levels under LPS-induced conditions (P<0.001). Moreover, at concentrations of 60~200 μg/mL, Isaria cicadae Miquel spore oil significantly promoted the proliferation of H₂O₂-induced Caco-2 cells (P<0.01), while concentrations of 40~100 μg/mL significantly enhanced the migration ability of Caco-2 cells (P<0.05). Thus, Isaria cicadae Miquel spore oil has the potential to promote the repair of oxidative damage to intestinal epithelial cells. As a component extracted from novel food materials, it holds promise for the development of foods for special medical purposes, targeting inflammatory bowel disease and intestinal mucosal injury.
- Isaria cicadae Miquel spore oil,
- the yield of oil,
- fatty acids,
- anti-inflammatory,
- oxidative damage

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