Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality

MA Kexin; XIANG Haiqiao; YU Xiao; PAN Deyin; LI Changbo; CHEN Hongsheng; DIAO Jingjing

doi:10.13386/j.issn1002-0306.2024040084

Volume 46 Issue 8

Apr. 2025

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Science and Technology of Food Industry > 2025 > 46(8): 43-51. > DOI: 10.13386/j.issn1002-0306.2024040084

MA Kexin, XIANG Haiqiao, YU Xiao, et al. Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality[J]. Science and Technology of Food Industry, 2025, 46(8): 43−51. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040084.

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Effects of Citrus Fiber and Inulin Addition on the Lamb Protein Structure and Mince Quality

MA Kexin^{1, 2,},
XIANG Haiqiao¹,
YU Xiao^{1, 2},
PAN Deyin^{1, 2, 3},
LI Changbo^{1, 2, 3},
CHEN Hongsheng^{1, 2, 3, ,},
DIAO Jingjing^4, ,

1.
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2.
Heilongjiang Food and Biotechnology Innovation and Research Center (International Cooperation), Daqing 163319, China
3.
Heilongjiang Bayi Agricultural University Mudanjiang Institute of Food and Biotechnology, Mudanjiang 157000, China
4.
National Coarse Grains Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China

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Received Date: April 08, 2024
Available Online: February 10, 2025

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Abstract

Abstract

In this study, the effects of adding citrus fiber (0.5%, 1.0%, 1.5%, and 2.0%) and inulin (2.0%, 3.0%, 4.0%, and 5.0%) were investigated on the lamb mince quality, water distribution, texture, the protein conformation and the microstructure of lamb protein. Results showed that adding 2.0% citrus fiber significantly increased the disulfide bond content (by 12%) and hydrophobic interactions (by 14%), along with increments in the β-folding content (increment of 8.14%) and irregular curling content (increment of 1.29%). The incorporation of 2.0% citrus fiber facilitated protein cross-linking through disulfide bonding, which elevated the immobilized water fraction. This structural modification promoted the development of a continuous three-dimensional protein network characterized by enhanced density and molecular ordering. The structural modifications induced by 2.0% citrus fiber supplementation significantly enhancd water-holding capacity and textural hardness within the meat matrix (P<0.05). The addition of 3.0% inulin significantly augmented β-sheet content by 8.68% (P<0.05) while inducing a marginal 0.31% increase in random coil structures, concomitant with a statistically significant improvement in water-holding capacity. However, unlike citrus fibers, 3.0% inulin addition promoted the exposure of tryptophan residues and had no significant effect on disulfide bonds and tyrosine residues (P>0.05). In summary, both 2.0% citrus fiber and 3.0% inulin supplementation demonstrated beneficial structural modifications in meat proteins, effectively improving water-holding capacity of minced meat and enhancing the quality of meat products.
- citrus fiber,
- inulin,
- lamb,
- protein structure,
- water retention

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References

[1]	王贵荣. 2023年农业经济形势总体良好[N]. 中国信息报, 2024-1-19. [WANG G R. The overall good agricultural economic situation in 2023[N]. China Information Daily, 2024-1-19.] WANG G R. The overall good agricultural economic situation in 2023[N]. China Information Daily, 2024-1-19.
[2]	李军, 金海. 2023年我国肉羊产业发展概况、未来发展趋势及建议[J]. 中国畜牧杂志,2024,60(3):322−328. [LI J, JIN H. Overview of the future development trends and suggestions for China's meat sheep industry in 2023[J]. Chinese Journal of Animal Husbandry,2024,60(3):322−328.] LI J, JIN H. Overview of the future development trends and suggestions for China's meat sheep industry in 2023[J]. Chinese Journal of Animal Husbandry, 2024, 60（3）: 322−328.
[3]	练佳韦, 刘颖春, 余红平. 结直肠癌的全球流行情况、危险因素及归因疾病负担研究进展[J]. 中国癌症防治杂志,2024,16(1):1−9. [LIAN J W, LIU Y C, YU H P. Colorectal cancer:global epidemiology, risk factors and advances in attributed disease burden[J]. Chinese Journal of Cancer Prevention and Treatment,2024,16(1):1−9.] doi: 10.3969/j.issn.1674-5671.2024.01.01 LIAN J W, LIU Y C, YU H P. Colorectal cancer: global epidemiology, risk factors and advances in attributed disease burden[J]. Chinese Journal of Cancer Prevention and Treatment, 2024, 16（1）: 1−9. doi: 10.3969/j.issn.1674-5671.2024.01.01
[4]	NIU Y G, FANG H C, HUO T Y, et al. A novel fat replacer composed by gelatin and soluble dietary fibers from black bean coats with its application in meatballs[J]. LWT-Food Science and Technology,2020,122:109000. doi: 10.1016/j.lwt.2019.109000
[5]	王昱, 袁晶晶, 赵电波, 等. 膳食纤维对低盐鸡胸肉糜凝胶特性的影响[J]. 食品科学,2023,44(8):16−22. [WANG Y, YUAN J J, ZHOU D B, et al. Effects of dietary fibers on gelation properties of Low-Salt chicken breast surimi[J]. Food Science,2023,44(8):16−22.] doi: 10.7506/spkx1002-6630-20220811-128 WANG Y, YUAN J J, ZHOU D B, et al. Effects of dietary fibers on gelation properties of Low-Salt chicken breast surimi[J]. Food Science, 2023, 44（8）: 16−22. doi: 10.7506/spkx1002-6630-20220811-128
[6]	杨光, 周茜, 董丽君, 等. 膳食纤维强化冻干山楂果对小鼠肠道健康的影响[J]. 现代食品科技,2016,32(12):20−25. [YANG G, ZHOU Q, DONG L J, et al. Effect of increased dietary fiber in freeze-dried hawthorn on the intestinal health of mice[J]. Modern Food Science and Technology,2016,32(12):20−25.] YANG G, ZHOU Q, DONG L J, et al. Effect of increased dietary fiber in freeze-dried hawthorn on the intestinal health of mice[J]. Modern Food Science and Technology, 2016, 32（12）: 20−25.
[7]	刘妍. 膳食纤维的功能及其在食品中的有效运用研究[J]. 现代食品,2021(12):56−57. [LIU Y. Study on the function of dietary fiber and its effective application in food[J]. Modern Food,2021(12):56−57.] LIU Y. Study on the function of dietary fiber and its effective application in food[J]. Modern Food, 2021（12）: 56−57.
[8]	任媛媛, 郝建雄, 李稳, 等. 化学法结合酶法提取牛蒡中的可溶性膳食纤维[J]. 中国食品添加剂,2017(1):77−82. [REN Y Y, HAO J X, LI W, et al. Study on the extraction of soluble dietary fiber by chemica-enzyme method from burdocks[J]. China Food Additives,2017(1):77−82.] doi: 10.3969/j.issn.1006-2513.2017.01.005 REN Y Y, HAO J X, LI W, et al. Study on the extraction of soluble dietary fiber by chemica-enzyme method from burdocks[J]. China Food Additives, 2017（1）: 77−82. doi: 10.3969/j.issn.1006-2513.2017.01.005
[9]	廉文涛, 黄雨洋, 李玉玲, 等. 膳食纤维对蛋白凝胶影响的研究进展[J]. 食品科学,2023,44(9):340−348. [LIAN W T, HUANG Y Y, LI Y L, et al. Advances in research on the effects of dietary fiber on protein gel properties[J]. Food Science,2023,44(9):340−348.] doi: 10.7506/spkx1002-6630-20220509-104 LIAN W T, HUANG Y Y, LI Y L, et al. Advances in research on the effects of dietary fiber on protein gel properties[J]. Food Science, 2023, 44（9）: 340−348. doi: 10.7506/spkx1002-6630-20220509-104
[10]	ZHOU F F, PAN M K, Ll Y, et al. Effects of Na⁺ on the cold gelation between a low-methoxyl pectin extracted from premna micophyla turcz and soy protein isolateyl[J]. Food Hydrocoloids,2020,104:105762. doi: 10.1016/j.foodhyd.2020.105762
[11]	ASHINSHANA U I, DINESH D J, CHENRUN J, et al. Inulin as a functional ingredient and their applications in meat products.[J]. Carbohydrate Polymers,2022,275:118706. doi: 10.1016/j.carbpol.2021.118706
[12]	王健, 张坤生, 任云霞. 柑橘纤维在低脂肉制品中的应用研究[J]. 中国食品添加剂,2010(6):204−208. [WANG J, ZHANG K S, REN Y X. The research on the application of citrus fiber in low-fat meat products[J]. China Food Additives,2010(6):204−208.] doi: 10.3969/j.issn.1006-2513.2010.06.034 WANG J, ZHANG K S, REN Y X. The research on the application of citrus fiber in low-fat meat products[J]. China Food Additives, 2010（6）: 204−208. doi: 10.3969/j.issn.1006-2513.2010.06.034
[13]	何雅静, 陈珊珊, 孙志高. 柑橘皮渣膳食纤维的特性及其在食品中的应用[J]. 食品工业科技,2021,42(19):436−442. [HE Y J, CHEN S S, SUN Z G. Characteristics of citrus peel dietary fiber and its application in food[J]. Science and Technology of Food Industry,2021,42(19):436−442.] HE Y J, CHEN S S, SUN Z G. Characteristics of citrus peel dietary fiber and its application in food[J]. Science and Technology of Food Industry, 2021, 42（19）: 436−442.
[14]	CRISTINA P, OLIVIER G, JANA H, et al. Effect of stabiliser classes (animal proteins, vegetable proteins, starches, hydrocolloids and dietary fibre) on the physicochemical properties of a model lean meat product[J]. International Journal of Food Science and Technology,2020,55(3):970−977. doi: 10.1111/ijfs.14354
[15]	TAMER E, ROWIDA E, BADIA B, et al. Effect of using chicory roots powder as a fat replacer on beef burger quality[J]. Slovenian Veterinary Research,2019,56(22):509−514.
[16]	XIN K, JI X Y, GUO Z L, et al. Pitaya peel extract and lemon seed essential oil as effective sodium nitrite replacement in cured mutton[J]. LWT-Food Science and Technology,2022,160:113283. doi: 10.1016/j.lwt.2022.113283
[17]	杨裕如, 潘德胤, 马金明, 等. 超声处理修饰羊肌肉蛋白结构改善嫩度的研究[J]. 食品工业科技,2023,44(21):45−53. [YANG Y R, PAN D Y, MA J M, et al. Study of modifying lamb muscle protein structure and improving the tenderness by ultrasonic treatment[J]. Science and Technology of Food Industry,2023,44(21):45−53.] YANG Y R, PAN D Y, MA J M, et al. Study of modifying lamb muscle protein structure and improving the tenderness by ultrasonic treatment[J]. Science and Technology of Food Industry, 2023, 44（21）: 45−53.
[18]	程天赋, 俞龙浩, 蒋奕, 等. 基于低场核磁共振探究解冻过程中肌原纤维水对鸡肉食用品质的影响[J]. 食品科学, 2019, 40(9):16−22. [CHENG T F, YU L H, JIANG Y, et al. Effect of myofibrillar water on chicken quality during thawing as studies by low-field nuclear magnetic resonance[J]. Food Science, 2019, 40(9):16−22.] CHENG T F, YU L H, JIANG Y, et al. Effect of myofibrillar water on chicken quality during thawing as studies by low-field nuclear magnetic resonance[J]. Food Science, 2019, 40（9）: 16−22.
[19]	张明成, 常光强, 王族, 等. 不同柑橘纤维及添加量对酱牛肉出品率及品质的影响[J]. 食品科学,2024,45(11):226−234. [ZHANG M C, CHANG G Q, WANG Z, et al. Effects of different citrus fiber and added amount on the cooking yield and quality of spiced beef[J]. Food Science,2024,45(11):226−234.] doi: 10.7506/spkx1002-6630-20231009-057 ZHANG M C, CHANG G Q, WANG Z, et al. Effects of different citrus fiber and added amount on the cooking yield and quality of spiced beef[J]. Food Science, 2024, 45（11）: 226−234. doi: 10.7506/spkx1002-6630-20231009-057
[20]	张杰, 唐善虎, 李思宁, 等. 添加含磷与非磷保水剂对牦牛肉肉糜保水性的影响[J]. 食品工业科技,2017,38(8):306−310. [ZHANG J, TANG S H, LI S N, et al. Effect of phosphorus-free agents on water-holding capacity of yak meat batters[J]. Science and Technology of Food Industry,2017,38(8):306−310.] ZHANG J, TANG S H, LI S N, et al. Effect of phosphorus-free agents on water-holding capacity of yak meat batters[J]. Science and Technology of Food Industry, 2017, 38（8）: 306−310.
[21]	张骏龙. 肉糜热诱导凝胶形成过程中水合特征变化规律研究[D]. 锦州:渤海大学, 2017. [ZHANG J L. Study on hydration characteristics of meat batters during heat-induced processing[D]. Jinzhou:Bohai University, 2017.] ZHANG J L. Study on hydration characteristics of meat batters during heat-induced processing[D]. Jinzhou: Bohai University, 2017.
[22]	望运滔, 王莎莎, 秦春艳, 等. 壳寡糖对高温条件下鸡胸肉糜凝胶品质的影响[J]. 食品科学,2023,44(22):49−54. [WANG Y T, WANG S S, QIN C Y, et al. Effect of chitooligosaccharide on the quality of gel of chicken breast meat mince under high temperature[J]. Food Science,2023,44(22):49−54.] doi: 10.7506/spkx1002-6630-20220403-031 WANG Y T, WANG S S, QIN C Y, et al. Effect of chitooligosaccharide on the quality of gel of chicken breast meat mince under high temperature[J]. Food Science, 2023, 44（22）: 49−54. doi: 10.7506/spkx1002-6630-20220403-031
[23]	殷玲, 牛德芳, 叶帅, 等. 巢脾多糖在调理低脂猪肉丸中的应用研究[J]. 食品研究与开发,2021,42(19):72−77. [YIN L, NIU D F, YE S, et al. Study on the application of Chaopi polysaccharide in conditioning low-fat pork balls[J]. Food Research and Development,2021,42(19):72−77.] doi: 10.12161/j.issn.1005-6521.2021.19.011 YIN L, NIU D F, YE S, et al. Study on the application of Chaopi polysaccharide in conditioning low-fat pork balls[J]. Food Research and Development, 2021, 42（19）: 72−77. doi: 10.12161/j.issn.1005-6521.2021.19.011
[24]	WANG Y, YUAN J J, LI K, et al. Effects of combined chickpea protein isolate and chitosan on the improvement of technological quality in phosphate-free pork meat emulsions:its relation to modifications on protein thermal and structural properties[J]. Meat Science,2023,201:109194. doi: 10.1016/j.meatsci.2023.109194
[25]	黄阮卿. 结冷胶复配体系对猪肉糜品质特性影响及其应用研究[D]. 杭州:浙江工商大学, 2023. [HUANG R Q. Effect of gellan gum compounding system on quality characteristics of minced pork and its application[D]. Hangzhou:Zhejiang Gongshang University, 2023.] HUANG R Q. Effect of gellan gum compounding system on quality characteristics of minced pork and its application[D]. Hangzhou: Zhejiang Gongshang University, 2023.
[26]	ZHU C Y, ZHANG S L, ZHU N, et al. Effects of citrus fiber on the emulsifying properties and molecular structure of mutton myofibrillar protein:an underlying mechanisms study[J]. Food Bioscience,2023,51:102304. doi: 10.1016/j.fbio.2022.102304
[27]	SONG J H, PAN T, WU J P, et al. The improvement effect and mechanism of citrus fiber on the water-binding ability of low-fat frankfurters[J]. Journal of Food Science and Technology,2016,53(12):4197−4204. doi: 10.1007/s13197-016-2407-5
[28]	王昱, 王家乐, 袁晶晶, 等. L-赖氨酸与谷氨酰胺转氨酶联合处理对低盐鸡肉糜凝胶保水及质构品质的影响[J]. 食品科学,2023,44(22):24−30. [WANG Y, WANG J L, YUAN J J, et al. Effects of combined treatment of L-lysine and transglutaminase on water-holding capacity and texture quality of low-salt chicken meat batter[J]. Food Science,2023,44(22):24−30.] doi: 10.7506/spkx1002-6630-20230115-115 WANG Y, WANG J L, YUAN J J, et al. Effects of combined treatment of L-lysine and transglutaminase on water-holding capacity and texture quality of low-salt chicken meat batter[J]. Food Science, 2023, 44（22）: 24−30. doi: 10.7506/spkx1002-6630-20230115-115
[29]	ZHANG Y M, DONG M, ZHANG X Y, et al. Effects of inulin on the gel properties and molecular structure of porcine myosin:A underlying mechanisms study[J]. Food Hydrocolloids,2020,108:105974. doi: 10.1016/j.foodhyd.2020.105974
[30]	SHAO J H, ZOU Y F, XU X L, et al. Evaluation of structural changes in raw and heated meat batters prepared with different lipids using raman spectroscopy[J]. Food Research International,2011,44(9):2955−2961. doi: 10.1016/j.foodres.2011.07.003
[31]	赵佳佳. 不同食盐含量对滑油后牛柳食用品质的影响[J]. 食品科技,2020,45(12):109−113. [ZHAO J J. Effects of different salt content on the edible quality of fried beef fillet[J]. Food Technology,2020,45(12):109−113.] ZHAO J J. Effects of different salt content on the edible quality of fried beef fillet[J]. Food Technology, 2020, 45（12）: 109−113.
[32]	李统帅, 兰梅娟, 李琳, 等. 多糖在鱼糜制品中的应用进展[J]. 中国食品添加剂,2024,35(3):281−288. [LI T S, LAN M J, LI L, et al. Application progress of ploysaccharides in surimi products[J]. China Food Additives,2024,35(3):281−288.] LI T S, LAN M J, LI L, et al. Application progress of ploysaccharides in surimi products[J]. China Food Additives, 2024, 35（3）: 281−288.
[33]	HAN K Y, LI S S, YANG Y L, et al. Mechanisms of inulin addition affecting the properties of chicken myofibrillar protein gel[J]. Food Hydrocolloids,2022,131:107843. doi: 10.1016/j.foodhyd.2022.107843
[34]	MCKENNA J P, JOSEPH G S, KENNETH J P, et al. Evaluation of citrus fiber as a natural replacer of sodium phosphate in alternatively-cured all-pork bologna sausage[J]. Meat Science,2019,157:107883. doi: 10.1016/j.meatsci.2019.107883
[35]	MENEGAS L Z, PIMENTEL T C, GARCIA S, et al. Dry-fermented chicken sausage produced with inulin and corn oil:physicochemical, microbiological, and textural characteristics and acceptability during storage[J]. Meat Science,2013,93(3):501−506. doi: 10.1016/j.meatsci.2012.11.003
[36]	张玉梅. 菊粉对低脂乳化肠食用品质影响及机制研究[D]. 南京:南京农业大学, 2020. [ZHANG Y M. Effect of inulin on edible quality of low-fat emulsified sausage and its mechanism[D]. Nanjing:Nanjing Agricultural University, 2020.] ZHANG Y M. Effect of inulin on edible quality of low-fat emulsified sausage and its mechanism[D]. Nanjing: Nanjing Agricultural University, 2020.

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