Effect of <i>Saccharomyces cerevisiae</i> L3 Protease on Quality Characteristics and Flavor of Harbin Dry Sausages

LIU Xinping; TIAN Jiaqi; WANG Lin; SUN Fangda; KONG Baohua

doi:10.13386/j.issn1002-0306.2024030358

Volume 46 Issue 5

Feb. 2025

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Science and Technology of Food Industry > 2025 > 46(5): 108-117. > DOI: 10.13386/j.issn1002-0306.2024030358

LIU Xinping, TIAN Jiaqi, WANG Lin, et al. Effect of Saccharomyces cerevisiae L3 Protease on Quality Characteristics and Flavor of Harbin Dry Sausages[J]. Science and Technology of Food Industry, 2025, 46(5): 108−117. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030358.

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Effect of Saccharomyces cerevisiae L3 Protease on Quality Characteristics and Flavor of Harbin Dry Sausages

School of Food Science, Northeast Agricultural University, Harbin 150030, China

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Received Date: March 24, 2024
Available Online: December 30, 2024

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Abstract

Abstract

This experiment aimed to examine how varying levels of Saccharomyces cerevisiae L3 protease impact protein hydrolysis, lipid oxidation, and the physicochemical traits of dry sausage during fermentation. The study also involved analyzing the quality of dry sausage using gas chromatography-mass spectrometry (GC-MS) and sensory evaluation techniques. The study found that S. cerevisiae L3 protease accelerated pH reduction in dry sausages. Compared with naturally fermented dry sausages, it accelerated the decrease in water content and water activity with increasing protease addition. At the same time, redness values (a^*) and shear were significantly higher when protease was added (P<0.05). In addition, S. cerevisiae L3 protease reduced lipid oxidation during fermentation of dry sausage. With the addition of S. cerevisiae L3 protease, the total free amino acid contents of dry sausage increased, while the formation of flavor substances was promoted. The results of sensory evaluation showed that the addition of 1.0 g/kg of S. cerevisiae L3 protease had the best improvement in odor and freshness of dry sausage and the best overall acceptability. In conclusion, S. cerevisiae L3 protease had a positive effect on improving the quality and flavor of fermented meat products.
- Saccharomyces cerevisiae,
- protease,
- Harbin dry sausage,
- quality characteristic,
- flavor

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References

[1]	刘思婷, 刘馨屿, 王雯萱, 等. 植物乳杆菌接种量对低盐风干肠品质特性及风味特征的影响[J]. 食品科学, 2022, 43(22):97−104. [LIU S T, LIU X Y, WANG W X, et al. Effect of different inoculum levels of Lactobacillus plantarum on quality characteristics and flavor profile of low-sodium dry sausage[J]. Food Science, 2022, 43(22):97−104.] LIU S T, LIU X Y, WANG W X, et al. Effect of different inoculum levels of Lactobacillus plantarum on quality characteristics and flavor profile of low-sodium dry sausage[J]. Food Science, 2022, 43（22）: 97−104.
[2]	HU Y Y, CHEN Q, WEN R X, et al. Quality characteristics and flavor profile of Harbin dry sausages inoculated with lactic acid bacteria and Staphylococcus xylosus[J]. LWT-Food Science and Technology,2019,114:108392. doi: 10.1016/j.lwt.2019.108392
[3]	CHEN Q, LIU Q, SUN Q, et al. Flavour formation from hydrolysis of pork sarcoplasmic protein extract by a unique LAB culture isolated from Harbin dry sausage[J]. Meat Science,2015,100:110−117. doi: 10.1016/j.meatsci.2014.10.001
[4]	刘天红, 纪蕾, 于晓清, 等. 沙蚕鲜味多肽复合酶解工艺优化及其风味表征[J]. 大连海洋大学学报, 2023, 38(5):857−865. [LIU T H, JI L, YU X Q, et al. Optimization of enzymatic hydrolysis process and sensory characterization for rag worm (Perinereis aibuhitensis) umami hydrolysate[J] Journal of Dalian Ocean University, 2023, 38(5):857−865.] LIU T H, JI L, YU X Q, et al. Optimization of enzymatic hydrolysis process and sensory characterization for rag worm （Perinereis aibuhitensis） umami hydrolysate[J] Journal of Dalian Ocean University, 2023, 38（5）: 857−865.
[5]	TANG T T, ZHANG M, LIU Y P. Valorization of meat and bone residue by ultrasound and high voltage electrostatic field assisted two-stage enzymatic hydrolysis:Nutritional characteristics and flavor analysis[J]. Food Bioscience,2023,56:103203. doi: 10.1016/j.fbio.2023.103203
[6]	CONTESINI F J, MELO R R D, SATO H H. An overview of Bacillus proteases:from production to application[J]. Critical Reviews in Biotechnology,2018,38(3):321−334. doi: 10.1080/07388551.2017.1354354
[7]	SHAN K, YAO Y Y, WANG J Y, et al. Effect of probiotic Bacillus cereus DM423 on the flavor formation of fermented sausage[J]. Food Research International,2023,172:113210−113210. doi: 10.1016/j.foodres.2023.113210
[8]	LI C S, CUI Q Y, LI L H, et al. Formation and improvement mechanism of physical property and volatile flavor of fermented tilapia surimi by newly isolated lactic acid bacteria based on two dimensional correlation networks[J]. Food Chemistry,2024,440:138260. doi: 10.1016/j.foodchem.2023.138260
[9]	LÓPEZ C C, PAPARELLA A, TOFALO R, et al. Proteolytic activity of Saccharomyces cerevisiae strains associated with Italian dry-fermented sausages in a model system[J]. International Journal of Food Microbiology,2011,150(1):50−58. doi: 10.1016/j.ijfoodmicro.2011.07.017
[10]	SUN F D, WANG H, LIU Q, et al. Proteolysis and quality characteristics of Harbin dry sausages caused by the addition of Staphylococcus xylosus protease[J]. Food Chemistry,2023,404(PB):134692.
[11]	WANG H P, SUI Y M, LIU J Q, et al. Analysis and comparison of the quality and flavour of traditional and conventional dry sausages collected from northeast China[J]. Food Chemistry:X,2023,20:100979.
[12]	WANG H, XU J H, LIU Q, et al. Effect of the protease from Staphylococcus carnosus on the proteolysis, quality characteristics, and flavor development of Harbin dry sausage[J]. Meat Science,2022,189:108827. doi: 10.1016/j.meatsci.2022.108827
[13]	CHEN Y W, CAI W Q, SHI Y G, et al. Effects of different salt concentrations and vacuum packaging on the shelf-stability of Russian sturgeon (Acipenser gueldenstaedti) stored at 4 ℃[J]. Food Control,2020,109:106865. doi: 10.1016/j.foodcont.2019.106865
[14]	DONG C H, SHI S, PAN N, et al. Inhibitory mechanism of tyramine-degrading strains on reducing tyramine accumulation in Harbin dry sausage during fermentation[J]. Food Control,2022,137:108952. doi: 10.1016/j.foodcont.2022.108952
[15]	LI Z Y, WANG J Y, ZHENG B D, et al. Impact of combined ultrasound-microwave treatment on structural and functional properties of golden threadfin bream (Nemipterus virgatus) myofibrillar proteins and hydrolysates[J]. Ultrasonics Sonochemistry,2020,65(prepublish):105063.
[16]	JU M, PIAO C X, ZHANG J, et al. Hydrolysis of pork sarcoplasmic protein extracts by unique staphylococci isolated from low-salt dry-cured ham[J]. LWT,2022,164:113639. doi: 10.1016/j.lwt.2022.113639
[17]	WEN R X, HU Y Y, ZHANG L, et al. Effect of NaCl substitutes on lipid and protein oxidation and flavor development of Harbin dry sausage[J]. Meat Science,2019,156:33−43. doi: 10.1016/j.meatsci.2019.05.011
[18]	朱嘉敏, 么紫瑶, 李梦彤, 等. 不同接种量的清酒乳杆菌对低钠盐风干肠品质的改善作用研究[J]. 食品工业科技,2023,44(7):133−142. [ZHU J M, MO Z Y, LI M T, et al. Study on different inoculation levels of Lactobacillus sakei on the improvement of quality characteristics of low-sodium dry sausages[J]. Science and Technology of Food Industry,2023,44(7):133−142.] ZHU J M, MO Z Y, LI M T, et al. Study on different inoculation levels of Lactobacillus sakei on the improvement of quality characteristics of low-sodium dry sausages[J]. Science and Technology of Food Industry, 2023, 44（7）: 133−142.
[19]	LEE J Y, KIM C J, KUNZ B. Identification of lactic acid bacteria isolated from kimchi and studies on their suitability for application as starter culture in the production of fermented sausages[J]. Meat Science,2005,72(3):437−445.
[20]	郑云, 郑爽, 周天硕, 等. 清酒乳杆菌对发酵风干肠品质的影响[J]. 现代食品科技,2023,39(12):122−129. [ZHENG Y, ZHENG S, ZHOU T S, et al. Effect of Lactobacillus sakeus on the quality of fermented air-dried sausage[J]. Modern food Science and Technology,2023,39(12):122−129.] ZHENG Y, ZHENG S, ZHOU T S, et al. Effect of Lactobacillus sakeus on the quality of fermented air-dried sausage[J]. Modern food Science and Technology, 2023, 39（12）: 122−129.
[21]	HAMOEN J R, VOLLEBREGT H M, SMAN R G M V D. Prediction of the time evolution of pH in meat[J]. Food Chemistry,2013,141(3):2363−2372. doi: 10.1016/j.foodchem.2013.04.127
[22]	HÜSEYIN B, MUSTAFA B. Colour and textural attributes of sucuk during ripening[J]. Meat Science,2006,73(2):344−350. doi: 10.1016/j.meatsci.2006.01.001
[23]	龚小会. 酵母产香特性及其对发酵肉制品风味品质的影响研究[D]. 贵阳:贵州大学, 2023. [GONG X H. Research on aroma-producing properties of yeast and its effect on flavor quality offermented meat products[D]. Guiyang:Guizhou University, 2023.] GONG X H. Research on aroma-producing properties of yeast and its effect on flavor quality offermented meat products[D]. Guiyang: Guizhou University, 2023.
[24]	HU Y Y, LI Y J, ZHU J M, et al. Improving the taste profile of reduced-salt dry sausage by inoculating different lactic acid bacteria[J]. Food Research International,2021,145:110391. doi: 10.1016/j.foodres.2021.110391
[25]	SUN Q, CHEN F S, GEN F, et al. A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides[J]. Food Chemistry,2018,245:570−577. doi: 10.1016/j.foodchem.2017.10.113
[26]	NIEVA E B, GOICOECHEA E, GUILLÉN M D. Effect of the presence of protein on lipolysis and lipid oxidation occurring during in vitro digestion of highly unsaturated oils[J]. Food Chemistry,2017,235:21−33. doi: 10.1016/j.foodchem.2017.05.028
[27]	ZHAO B, ZHOU H M, ZHANG S L, et al. Changes of protein oxidation, lipid oxidation and lipolysis in Chinese dry sausage with different sodium chloride curing salt content[J]. Food Science and Human Wellness,2020,9(4):328−337. doi: 10.1016/j.fshw.2020.04.013
[28]	XIA L Y, QIAN M, CHENG F, et al. The effect of lactic acid bacteria on lipid metabolism and flavor of fermented sausages[J]. Food Bioscience,2023,56:103172. doi: 10.1016/j.fbio.2023.103172
[29]	CAI W W, HU X M, WANG Y M, et al. Bioactive peptides from tuna cardiac arterial bulbs:Preparation, identification, antioxidant activity and stability against thermal, pH and simulated gastrointestinal digestion treatments[J]. Marine Drugs,2022,20(10):626. doi: 10.3390/md20100626
[30]	YU D, FENG M Q, SUN J. Influence of mixed starters on the degradation of proteins and the formation of peptides with antioxidant activities in dry fermented sausages[J]. Food Control,2021,123:107743. doi: 10.1016/j.foodcont.2020.107743
[31]	XING L J, HU Y Y, HU H Y, et al. Purification and identification of antioxidative peptides from dry-cured Xuanwei ham[J]. Food Chemistry,2016,194:951−958. doi: 10.1016/j.foodchem.2015.08.101
[32]	TIMÓN M L, BRONCANO J M, ANDRÉS A I, et al. Prevention of rancidity and discolouration of Iberian dry cured sausage using proteases[J]. LWT-Food Science and Technology,2014,58(1):293−298. doi: 10.1016/j.lwt.2014.02.042
[33]	LIU Y, ZHANG Y Y, LONG F W, et al. Effect of tea-polyphenol/β-cyclodextrin/NaCl inclusion complexes as a salt substitute on quality of low-salt Sichuan-style sausages[J]. LWT,2023,188:115328. doi: 10.1016/j.lwt.2023.115328
[34]	SANTOS N N, MENDONÇA R C S, SANZ Y, et al. Hydrolysis of pork muscle sarcoplasmic proteins by Debaryomyces hansenii[J]. International Journal of Food Microbiology,2001,68(3):199−206. doi: 10.1016/S0168-1605(01)00489-5
[35]	ZHENG S S, WANG C Y, HU Y Y, et al. Enhancement of fermented sausage quality driven by mixed starter cultures:Elucidating the perspective of flavor profile and microbial communities[J]. Food Research International,2024,178:113951. doi: 10.1016/j.foodres.2024.113951
[36]	OLIVARES A, NAVARRO J L, FLORES M. Effect of fat content on aroma generation during processing of dry fermented sausages[J]. Meat Science,2010,87(3):264−273.
[37]	ZHANG K, ZHANG T T, GUO R R, et al. The regulation of key flavor of traditional fermented food by microbial metabolism:A review[J]. Food Chemistry:X,2023,19:100871.
[38]	FLORES M, CORRAL S, GARCÍA L C, et al. Yeast strains as potential aroma enhancers in dry fermented sausages[J]. International Journal of Food Microbiology,2015,212:16−24. doi: 10.1016/j.ijfoodmicro.2015.02.028
[39]	WEN R X, SUN F D, LI X A, et al. The potential correlations between the fungal communities and volatile compounds of traditional dry sausages from Northeast China[J]. Food Microbiology,2021,98:103787. doi: 10.1016/j.fm.2021.103787
[40]	GAO F, ZHANG K P, WANG D X, et al. Effect of Lactobacillus helveticus IMAUJBH1 on fat and volatile flavor substances in fermented mutton sausages[J]. Food Chemistry:X,2024,21:101205.
[41]	WANG X P, ZHOU P F, CHENG J R, et al. The role of endogenous enzyme from straw mushroom (Volvariella volvacea) in improving taste and volatile flavor characteristics of Cantonese sausage[J]. LWT,2022,154:112627.
[42]	GONG X H, CHEN X, MI R F, et al. Two Debaryomyces hansenii strains as starter cultures for improving the nutritional and sensory quality of dry-cured pork belly[J]. Food Research International,2024,183:114227. doi: 10.1016/j.meatsci.2016.08.007