YANG Hongyan, HE Yuqi, NIU Miao, et al. Analysis of Aroma Composition in Pu-erh Ripen Tea with Different Producing Areas and Storage Durations[J]. Science and Technology of Food Industry, 2025, 46(5): 218−229. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030016.
Citation: YANG Hongyan, HE Yuqi, NIU Miao, et al. Analysis of Aroma Composition in Pu-erh Ripen Tea with Different Producing Areas and Storage Durations[J]. Science and Technology of Food Industry, 2025, 46(5): 218−229. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030016.

Analysis of Aroma Composition in Pu-erh Ripen Tea with Different Producing Areas and Storage Durations

More Information
  • Received Date: March 04, 2024
  • Available Online: January 05, 2025
  • Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and sensory evaluation were used to investigate the aroma characteristics of Pu-erh ripen tea from different production areas and stored for different periods. Twelve samples of Pu-erh ripen tea from four areas, namely, Dehong Mangshi, Lincang Shuangjiang, Xishuangbanna Menghai, and Pu'er Lancang with stored times of 3 years, 8 years, and 13 years were evaluated. A total of 103 aroma components were identified. The levels of alcohols were reduced in the tea samples from Pu'er Lancang and Lincang Shuangjiang, together with reductions in the flower and fruit aromas, in contrast to the samples from Dehong Mangshi and Xishuangbanna Menghai where these aromas were increased, especially the flower aromas. It was found that the contents of 1,2,3-trimethoxybenzene, 1,2,4-trimethoxybenzene increased in correspondence with the storage duration in samples from the different producing areas. Multivariate statistical analysis and odor activity values showed that 11 aroma components, including benzaldehyde, octanoic acid, 2-heptanone, and valeraldehyde, differed significantly between the different producing areas. Overall, 28, 30, 39, and 39 different types of aroma components were found in association with storage time in the samples from selected from samples from Dehong Mangshi, Lincang Shuangjiang, Pu'er Lancang, and Xishuangbanna Menghai, respectively. A total of 15 aroma compounds with OAV>1, and (E,Z)-2,6-nonadienal, (E)-2-nonena, and 1,2,3-trimethoxybenzene with OAV>100, were found to be the key aroma components in the Pu-erh Ripen tea samples, while 2-ethylhexanol, octanal, and α-lononethere showed significant differences between different producing areas. The findings of the study reveal the characteristics of the aroma quality and provide a theoretical basis and data support for an accurate understanding of the aroma composition and changes in Pu-erh ripen tea from different producing areas and storage durations.
  • [1]
    DENG X, ZHANG N, WANG Q, et al. Theabrownin of raw and ripened Pu-erh tea varies in the alleviation of HFD-induced obesity via the regulation of gut microbiota[J]. European Journal of Nutrition,2023,62(5):2177−2190. doi: 10.1007/s00394-023-03089-w
    [2]
    ZHOU B, MA B, MA C, et al. Classification of Pu-erh ripened teas and their differences in chemical constituents and antioxidant capacity[J]. LWT-Food Science and Technology,2022,153:112370. doi: 10.1016/j.lwt.2021.112370
    [3]
    国家标准化管理委员会. GB/T 22111-2008 地理标志产品 普洱茶[S]. 北京:中国标准出版社, 2008. [Standardization Administration of the People's Republic of China. GB/T 22111-2008 Product of geograical indication-Puer tea[S]. Beijing:Standards Press of China, 2008.]

    Standardization Administration of the People's Republic of China. GB/T 22111-2008 Product of geograical indication-Puer tea[S]. Beijing: Standards Press of China, 2008.
    [4]
    许姗姗. 储藏环境对普洱茶化学品质的影响[D]. 合肥:安徽农业大学, 2019:1−2. [XU S S. Effects on chemicaal quality of Pu-erh tea with different storage enviroment[D]. Hefei:Anhui Agricultural University, 2019:1−2.]

    XU S S. Effects on chemicaal quality of Pu-erh tea with different storage enviroment[D]. Hefei: Anhui Agricultural University, 2019: 1−2.
    [5]
    DU L, WANG C, ZHANG C, et al. Characterization of the volatile and sensory profile of instant Puerh tea using GC×GC-TOFMS and descriptive sensory analysis[J]. Microchemical Journal,2019,146:986−996. doi: 10.1016/j.microc.2019.02.036
    [6]
    YE J, WANGA W G, HO C, et al. Differentiation of two types of pu-erh teas by electronic nose and ultrasound-assisted extraction-dispersive liquid-liquid microextraction-gas chromatography-mass spectrometry[J]. Analytical Methods,2015,8(3):2−47.
    [7]
    WANG C, LI J, WU X, et al. Pu-erh tea unique aroma:volatile components, evaluation methods and metabolic mechanism of key odor-active compounds[J]. Trends in Food Science & Technology,2022,124:25−37.
    [8]
    WANG Z, SU D, REN H, et al. Effect of different drying methods after fermentation on the aroma of Pu-erh tea (ripe tea)[J]. LWT-Food Science and Technology,2022,171:114129. doi: 10.1016/j.lwt.2022.114129
    [9]
    TIAN D, HUANG G, REN L, et al. Effects of Monascus purpureus on ripe Pu-erh tea in different fermentation methods and identification of characteristic volatile compounds[J]. Food Chemistry,2024,440:138249. doi: 10.1016/j.foodchem.2023.138249
    [10]
    GAO L, BIAN M X, MI R F, et al. Quality identification and evaluation of Pu-erh teas of different grade levels and various ages through sensory evaluation and instrumental analysis[J]. International Journal of Food Science and Technology 2016, 51:1338−1348.
    [11]
    杨雪梅, 任洪涛, 罗琼仙, 等. ‘紫娟’红茶和‘紫娟’普洱熟茶香气成分的分析[J]. 热带农业科学,2017,37(5):72−82. [YANG X M, REN H T, LUO Q X, et al. Aromatic constituents of 'Zijuan' black tea and 'Zijuan' Pu-erh tea[J]. Chinese Journal of Tropical Agriculture,2017,37(5):72−82.]

    YANG X M, REN H T, LUO Q X, et al. Aromatic constituents of 'Zijuan' black tea and 'Zijuan' Pu-erh tea[J]. Chinese Journal of Tropical Agriculture, 2017, 37(5): 72−82.
    [12]
    赵苗苗, 严亮, 张文杰, 等. 红紫芽熟茶适制性及渥堆发酵过程品质变化研究[J]. 食品安全质量检测学报, 2022, 13(19):6212−6220. [ZHAO M M, YAN L, ZHANG W J, et al. Study on the suitability of purple bud post-fermented Pu-erh tea and quality changes during the fermentation process[J]. Journal of Food Safety and Quality, 2022, 13(19):6212−6220.]

    ZHAO M M, YAN L, ZHANG W J, et al. Study on the suitability of purple bud post-fermented Pu-erh tea and quality changes during the fermentation process[J]. Journal of Food Safety and Quality, 2022, 13(19): 6212−6220.
    [13]
    苟祎, 夏丽飞, 张艳梅, 等. 基于HS-SPME-GC-MS的不同品种普洱熟茶香气成分的比较分析[J]. 现代食品科技,2023,39(10):1−11. [JU W, XIA L F, ZHANG Y M, et al. Comparative analysis of aroma components of different varieties of Pu-erh Ripen tea based on HS-SPME-GC-MS[J]. Modern Food Science and Technology,2023,39(10):1−11.]

    JU W, XIA L F, ZHANG Y M, et al. Comparative analysis of aroma components of different varieties of Pu-erh Ripen tea based on HS-SPME-GC-MS[J]. Modern Food Science and Technology, 2023, 39(10): 1−11.
    [14]
    张纪伟, 沈雪梅, 张钎, 等. 不同产地和贮存年份普洱生茶香气和呈味物质变化的比较研究[J]. 食品研究与开发,2021,42(9):11−18. [ZHANG J W, SHEN X M, ZHANG Q, et al. Comparative study on changes of aroma and flavor components of raw Pu-erh tea produced in different producing areas and storage years[J]. Food Research and Development,2021,42(9):11−18.]

    ZHANG J W, SHEN X M, ZHANG Q, et al. Comparative study on changes of aroma and flavor components of raw Pu-erh tea produced in different producing areas and storage years[J]. Food Research and Development, 2021, 42(9): 11−18.
    [15]
    赵阳, 龚加顺, 王秋萍. 古树普洱茶生茶贮藏过程中香气成分的变化[J]. 食品科学,2022,43(4):241−248. [ZHAO Y, GONG J S, WANG Q P. Change in aroma components of raw Pu-erh tea from ancient tea trees during storag

    J]. Food Science,2022,43(4):241−248.
    [16]
    YANG Y, RONG Y, LIU F, et al. Rapid characterization of the volatile profiles in Pu-erh tea by gas phase electronic nose and microchamber/thermal extractor combined with TD-GC-MS[J]. J Food Sci,2021,86(6):2358−2373. doi: 10.1111/1750-3841.15723
    [17]
    张晨霞, 王国成, 王超, 等. 普洱市不同产茶区普洱生茶香气成分差异性分析[J]. 食品研究与开发,2020,41(1):177−184. [ZHANG C X, WANG G C, WANG C, et al. Comparative study on the aroma components of raw Pu-erh tea among different producing areas of Pu'er city[J]. Food Research and Development,2020,41(1):177−184.]

    ZHANG C X, WANG G C, WANG C, et al. Comparative study on the aroma components of raw Pu-erh tea among different producing areas of Pu'er city[J]. Food Research and Development, 2020, 41(1): 177−184.
    [18]
    罗美玲, 田洪敏, 杨雪梅, 等. 电子鼻技术对普洱熟茶香气判别的研究[J]. 西南大学学报(自然科学版),2018,40(8):16−24. [LUO M L, TIAN H M, YANG X M, et al. Discriminamt research for identifying aromas of post-fermened Pu-erh tea from different storage years using an electronic nose[J]. Journal of Southwest University (Natureal Science Edition),2018,40(8):16−24.]

    LUO M L, TIAN H M, YANG X M, et al. Discriminamt research for identifying aromas of post-fermened Pu-erh tea from different storage years using an electronic nose[J]. Journal of Southwest University (Natureal Science Edition), 2018, 40(8): 16−24.
    [19]
    RONG Y, XIE J, YUAN H, et al. Characterization of volatile metabolites in Pu-erh teas with different storage years by combining GC-E-Nose, GC-MS, and GC-IMS[J]. Food Chemistry:X,2023,25(18):100693.
    [20]
    YANG X, LIU Y, MU L, et al. Discriminant research for identifying aromas of non-fermented Pu-erh tea from different storage years using an electronic nose[J]. Journal of Food Processing and Preservation,2018,42(10):e13721. doi: 10.1111/jfpp.13721
    [21]
    国家标准化管理委员会, 国家质量监督检验检疫总局. GB/T 23776-2018 茶叶感官审评方法[S]. 北京:中国标准出版社, 2018. [Standardization Administration of the People's Republic of China, General Administration of Quality Supervision, Inspection and Quarantine. GB/T 23776-2018 Methodology for sensory evaluation of tea[S]. Beijing:Standards Press of China, 2018.]

    Standardization Administration of the People's Republic of China, General Administration of Quality Supervision, Inspection and Quarantine. GB/T 23776-2018 Methodology for sensory evaluation of tea[S]. Beijing: Standards Press of China, 2018.
    [22]
    魏昊, 蓝天梦, 缪伊雯, 等. 基于感官组学分析不同足火方式对金牡丹工夫红茶香气的影响[J]. 茶叶科学,2023,43(1):109−123. [WEI H, LAN T M, MIAO Y W, et al. Analysis of the effect of different full firing methods on the aroma of Jinmudan Congou black tea based on sensomics characterization[J]. Journal of Tea Science,2023,43(1):109−123.]

    WEI H, LAN T M, MIAO Y W, et al. Analysis of the effect of different full firing methods on the aroma of Jinmudan Congou black tea based on sensomics characterization[J]. Journal of Tea Science, 2023, 43(1): 109−123.
    [23]
    DENG X, HUANG G, TU Q, et al. Evolution analysis of flavor-active compounds during artificial fermentation of Pu-erh tea[J]. Food Chemistry,2021,357:129783. doi: 10.1016/j.foodchem.2021.129783
    [24]
    邓见田烨, 晏美红, 尚铂昊, 等. 基于HS-SPME-GC-MS技术分析不同种类黑茶香气成分[J]. 食品工业科技, 2023, 44(18):378-386. [DENG J T Y, YAN M H, SHANG B H, et al. Study on aroma components in different types of dark tea based on HS-SPME-GC-MS[J]. Science and Technology of Food Industry, 2023, 44(18):378−386.
    [25]
    YANG P, SONG H, LIN Y, et al. Differences of characteristic aroma compounds in Rougui tea leaves with different roasting temperatures analyzed by switchable GC-O-MS and GC×GC-O-MS and sensory evaluation[J]. Food & Function,2021,12(11):4797−4807.
    [26]
    NIE C N, ZHONG X X, HE L, et al. Comparison of diferent aroma-active compounds of Sichuan dark brick tea (Camellia sinensis) and Sichuan Fuzhuan brick tea using gas chromatography-mass spectrometry (GC-MS) and aroma descriptive profle tests[J]. European Food Research and Technology,2019,245:1963−1979. doi: 10.1007/s00217-019-03304-1
    [27]
    YE J, WANG Y, LIN S, et al. Effect of processing on aroma intensity and odor characteristic of Shuixian(Camellia sinensis) tea[J]. Food Chemistry:X,2023,17:100616.
    [28]
    王娟, 肖巧梅, 张晓宇, 等. 不同等级普洱熟茶的代谢物差异分析[J]. 现代食品科技,2022,38(11):122−131. [WANG J, XIAO Q M, ZHANG X Y, et al. Analysis of differentially accumulated metabolites among different grades of ripe Pu-erh tea[J]. Modern Food Science and Technology,2022,38(11):122−131.]

    WANG J, XIAO Q M, ZHANG X Y, et al. Analysis of differentially accumulated metabolites among different grades of ripe Pu-erh tea[J]. Modern Food Science and Technology, 2022, 38(11): 122−131.
    [29]
    SHEM W J, 李亚莉, 周红杰, 等. 不同容器贮藏对普洱熟茶品质的影响[J]. 食品安全质量检测学报, 2022, 13(9):2763−2769. [SHEM W J, LI Y L, ZHOU H J, et al. Effects of different storage containers on the quality of fermented Pu-erh tea[J]. Journal of Food Safety and Quality, 2022, 13(9):2763−2769.]

    SHEM W J, LI Y L, ZHOU H J, et al. Effects of different storage containers on the quality of fermented Pu-erh tea[J]. Journal of Food Safety and Quality, 2022, 13(9): 2763−2769.
    [30]
    LÜ H, ZHONG Q, LIN Z, et al. Aroma characterisation of Pu-erh tea using headspace-solid phase microextraction combined with GC/MS and GC-olfactometry[J]. Food Chemistry, 2012, 130(4):1074−1081.
    [31]
    WANG C, HE Z, ZHANG C, et al. Sensory and instrumental analysis-guided exploration of odor-active compounds recovery with oil during the water-boiling extraction of Pu-erh tea[J]. Food Research International,2020,134:109243. doi: 10.1016/j.foodres.2020.109243
    [32]
    曹艳妮, 刘通讯. 不同储存时间普洱生茶和熟茶香气成分分析[J]. 食品工业,2011(10):64−67. [CAO Y N, LIU T X. Analysis of aroma composition in Pu-erh raw and ripe teas with different storage time[J]. Science and Technology,2011(10):64−67.]

    CAO Y N, LIU T X. Analysis of aroma composition in Pu-erh raw and ripe teas with different storage time[J]. Science and Technology, 2011(10): 64−67.
    [33]
    CHEN X, CHEN D, JIANG H, et al. Aroma characterization of Hanzhong black tea (Camellia sinensis) using solid phase extraction coupled with gas chromatography-mass spectrometry and olfactometry and sensory analysis[J]. Food Chemistry,2018,274:130−136.
    [34]
    王梦琪, 朱荫, 张悦, 等. “清香”绿茶的挥发性成分及其关键香气成分分析[J]. 食品科学, 2019, 40(22):219−228. [WANG M Q, ZHU Y, ZHANG Y, et al. Analysis of volatile composition and key aroma compounds of green teas with fresh scent flavor[J]. Food Science, 2019, 40(22):219−228.]

    WANG M Q, ZHU Y, ZHANG Y, et al. Analysis of volatile composition and key aroma compounds of green teas with fresh scent flavor[J]. Food Science, 2019, 40(22): 219−228.
    [35]
    郑鹏程, 刘盼盼, 王胜鹏, 等. 5种黑茶香气成分的比较分析[J]. 食品工科技,2018,39(22):82−86,143. [ZHENG P C, LIU P P, WANG S P, et al. Comparative analysis of the aroma components in five kinds of dark tea[J]. Science and Technology of Food Industry,2018,39(22):82−86,143.]

    ZHENG P C, LIU P P, WANG S P, et al. Comparative analysis of the aroma components in five kinds of dark tea[J]. Science and Technology of Food Industry, 2018, 39(22): 82−86,143.
    [36]
    石禾云, 杨豆豆, 张娅, 等. 普洱熟茶关键香气物质热释放规律分析[J]. 食品科学, 2023, 44(16):243−250. [SHI H Y, YANG D D, ZHANG Y, et al. Analysis of heat release law of key aroma substances in Pu-erh ripe tea[J]. Food Science, 2023, 44(16):243−250.]

    SHI H Y, YANG D D, ZHANG Y, et al. Analysis of heat release law of key aroma substances in Pu-erh ripe tea[J]. Food Science, 2023, 44(16): 243−250.
    [37]
    ZHANG W, CAO J, LI Z, et al. HS-SPME and GC/MS volatile component analysis of Yinghong No. 9 dark tea during the pile fermentation process[J]. Food Chemistry,2021,357:129654. doi: 10.1016/j.foodchem.2021.129654
    [38]
    LI Q, LI Y, LUO Y, et al. Characterization of the key aroma compounds and microorganisms during the manufacturing process of Fu brick tea[J]. LWT-Food Science and Technology,2020,127:109355. doi: 10.1016/j.lwt.2020.109355
    [39]
    吕世懂, 孟庆雄, 徐咏全, 等. 普洱茶香气分析方法及香气活性物质研究进展[J]. 食品科学,2014,35(11):292−298. [LÜ S D, MENG Q X, XU Y Q, et al. Recent progress in aroma analysis methods and aroma active compounds in Pu-erh tea[J]. Food Science,2014,35(11):292−298.]

    LÜ S D, MENG Q X, XU Y Q, et al. Recent progress in aroma analysis methods and aroma active compounds in Pu-erh tea[J]. Food Science, 2014, 35(11): 292−298.
    [40]
    王志霞. 普洱茶(熟茶)陈香特征物质及关键香气成分研究[D]. 昆明:云南农业大学, 2023. [WANG Z X. Study on characteristic substances and key aroma components of Pu-erh tea(ripe tea)[D]. Kunming:Yuannan Agricultural University, 2023.]

    WANG Z X. Study on characteristic substances and key aroma components of Pu-erh tea(ripe tea)[D]. Kunming: Yuannan Agricultural University, 2023.
    [41]
    曹艳妮, 刘通讯. 普洱生茶和熟茶香气中萜烯类和甲氧基苯类成分分析[J]. 食品工业科技,2012,33(5):128−130. [CAO Y N, LIU T X. Analysis of lerpenes and methoxybenzene components in aroma composition of pu-erh raw tea and ripe tea[J]. Science and Technology of Food Industry,2012,33(5):128−130.]

    CAO Y N, LIU T X. Analysis of lerpenes and methoxybenzene components in aroma composition of pu-erh raw tea and ripe tea[J]. Science and Technology of Food Industry, 2012, 33(5): 128−130.
    [42]
    孙振杰, 王梦馨, 崔林, 等. 普洱茶香气成分研究进展[J]. 茶叶通讯,2020,47(1):13−19. [SUN Z J, WANG M X, CUI L, et al. Research progress on aroma components in Pu'er tea[J]. Journal of Tea Communication,2020,47(1):13−19.]

    SUN Z J, WANG M X, CUI L, et al. Research progress on aroma components in Pu'er tea[J]. Journal of Tea Communication, 2020, 47(1): 13−19.
    [43]
    WU Y, LÜ S, WANG C, et al. Comparative analysis of volatiles difference of Yunnan sun-dried Pu-erh green tea from different tea mountains:Jingmai and Wuliang mountain by chemical fingerprint similarity combined with principal component analysis and cluster analysis[J]. BMC Chemistry,2016,10(1):11.
    [44]
    ZHENG X, LI Q, XIANG L, et al. Recent advances in volatiles of teas[J]. Molecules,2016,21(3):338. doi: 10.3390/molecules21030338
  • Other Related Supplements

Catalog

    Article Metrics

    Article views (68) PDF downloads (17) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return