Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats

MENG Weihong; LI Zhiming; ZHANG Jiayu; SHU Xin; LIANG Defu; ZHUANG Kejin; ZHANG Dongjie

doi:10.13386/j.issn1002-0306.2024010212

Volume 46 Issue 2

Jan. 2025

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2025 > 46(2): 324-333. > DOI: 10.13386/j.issn1002-0306.2024010212

MENG Weihong, LI Zhiming, ZHANG Jiayu, et al. Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats[J]. Science and Technology of Food Industry, 2025, 46(2): 324−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024010212.

Citation:

PDF (8674 KB)

Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats

MENG Weihong^1,,
LI Zhiming¹,
ZHANG Jiayu¹,
SHU Xin¹,
LIANG Defu¹,
ZHUANG Kejin^{1, 2, 3, ,},
ZHANG Dongjie^{1, 2, 3, ,}

1.
Heilongjiang Bayi Agricultural University, College of Food Science and Technology, Daqing 163319, China
2.
National Coarse Grain Engineering Research and Technology Center, Daqing 163319, China
3.
Heilongjiang Key Laboratory of Agricultural Products Processing and Quality Safety, Daqing 163319, China

More Information

Received Date: January 23, 2024
Available Online: November 13, 2024

Graphical Abstract

Abstract

Abstract

To explore the protective effects of a black bean-whey double protein (B-W double protein, B-W DP) diet on intestinal barrier injury induced by lipopolysaccharides (LPS) in rats. Sprague-Dawley rats were fed low, medium, and high doses of B-W DP for 28 d, and then, LPS was used to induce intestinal barrier injury. The intestinal morphology of the rats was observed using hematoxylin and eosin staining. The expression of tight junction proteins was quantified using immunohistochemistry, and the TLR4 and MyD88 expression levels were determined using real-time fluorescence quantitative polymerase chain reaction. Results showed that compared with low- and high-dose B-W DP, intermediate-dose B-W DP significantly (P<0.01) protected against intestinal barrier injury caused by LPS, increased villus length by 26.69%, reduced crypt depth by 22.61%, and increased their ratio by 46.78%. Three doses of B-W DP regulated tight junction proteins in different manners, and the intermediate dose exhibited the most significant effect (P<0.01), increasing ZO-1, Claudin-1, and Occludin expression levels by 14.32%, 31.80%, and 16.67%, respectively. Three doses of B-W DP significantly inhibited the inflammatory pathway (P<0.01), and the intermediate dose exhibited the most significant effect, reducing TLR4 and MyD88 protein levels by 37.25% and 33.04%, respectively. In summary, a moderate dose of B-W DP can improve intestinal morphology, intestinal digestion, and absorption capacity, increase the expression of tight junction proteins, maintain the integrity of epithelial cells, reduce intestinal permeability, downregulate TLR4 and MyD88 protein expression, and alleviate inflammatory reactions. All of which exhibit effective protective effects on LPS-induced intestinal barrier injury.
- black bean-whey double protein,
- lipopolysaccharide,
- intestinal barrier injury,
- intestinal morphology,
- tight junction protein

FullText(HTML)

References (42)

References

[1]	包云丽, 汪哲, 唐海茹, 等. 1990-2019年中国炎症性肠病疾病负担及变化趋势分析[J]. 中国全科医学,2023,26(36):4581−4586. [BAO Y L, WANG Z, TANG H R, et al. Disease burden and variation tendency of inflammatory bowel disease in China from 1990 to 2019[J]. Chinese General Practice,2023,26(36):4581−4586.] BAO Y L, WANG Z, TANG H R, et al. Disease burden and variation tendency of inflammatory bowel disease in China from 1990 to 2019[J]. Chinese General Practice, 2023, 26（36）: 4581−4586.
[2]	吴国豪. 肠道屏障功能[J]. 肠外与肠内营养,2004,11(1):44−47. [WU G H. Intestinal barrier function[J]. Parenteral & Enteral Nutrition,2004,11(1):44−47.] doi: 10.3969/j.issn.1007-810X.2004.01.014 WU G H. Intestinal barrier function[J]. Parenteral & Enteral Nutrition, 2004, 11（1）: 44−47. doi: 10.3969/j.issn.1007-810X.2004.01.014
[3]	ENAUD R, HOOKS B K, BARRE A, et al. Intestinal inflammation in children with cystic fibrosis is associated with crohn’s-like microbiota disturbances[J]. Journal of Clinical Medicine,2019,8(5):645. doi: 10.3390/jcm8050645
[4]	ZHANG K Y, HORNEF M W, DUPONT A. The intestinal epithelium as guardian of gut barrier integrity[J]. Cellular Microbiology,2015,17(11):1561−1569. doi: 10.1111/cmi.12501
[5]	VANCAMELBEKE, VERMEIRE M, SEVERINE. The intestinal barrier:a fundamental role in health and disease[J]. Expert Review of Gastroenterology & Hepatology,2017,11(7/12):821−834.
[6]	姚鹏, 郝娜. 肠屏障功能与消化系统疾病关系的研究进展[J]. 中国中西医结合外科杂志,2021,27(5):785−787. [YAO P, HAO N. Research progress on the relationship between intestinal barrier function and digestive system diseases[J]. Chinese Journal of Surgery of Integrated Traditional and Western Medicine,2021,27(5):785−787.] doi: 10.3969/j.issn.1007-6948.2021.05.023 YAO P, HAO N. Research progress on the relationship between intestinal barrier function and digestive system diseases[J]. Chinese Journal of Surgery of Integrated Traditional and Western Medicine, 2021, 27（5）: 785−787. doi: 10.3969/j.issn.1007-6948.2021.05.023
[7]	褚宇瑶, 戴岳. 肠黏膜屏障及其与多种疾病相关性的研究进展[J]. 中国现代医药杂志,2020,22(7):102−105. [CHU Y Y, DAI Y. Research progress of intestinal mucosal barrier and its correlation with many diseases[J]. Modern Medicine Journal of China,2020,22(7):102−105.] CHU Y Y, DAI Y. Research progress of intestinal mucosal barrier and its correlation with many diseases[J]. Modern Medicine Journal of China, 2020, 22（7）: 102−105.
[8]	NAGPAL R, YADAV H, et al. Bacterial translocation from the gut to the distant organs:An overview[J]. Annals of Nutrition & Metabolism,2017,71(Suppl.1):11−16.
[9]	SUPAJATURA V, USHIO H, NAKAO A, et al. Differential responses of mast cell Toll-like receptors 2 and 4 in allergy and innate immunity[J]. Journal of Clinical Investigation,2002,109(10):1351−1359. doi: 10.1172/JCI0214704
[10]	DEGUINE J, BARTON M G. MyD88:A central player in innate immune signaling[J]. F1000 Prime Reports,2014,6:97.
[11]	LI R N, HONG P, ZHENG X. β-carotene attenuates lipopolysaccharide-induced inflammation via inhibition of the NF-κB, JAK2/STAT3 and JNK/p38 MAPK signaling pathways in macrophages[J]. Animal Science Journal,2019,90(1):140−148. doi: 10.1111/asj.13108
[12]	NAVIS M, MUNCAN V, SANGILD T P, et al. Beneficial effect of mildly pasteurized whey protein on intestinal integrity and innate defense in preterm and near-term piglets[J]. Nutrients,2020,12(4):1125−1125. doi: 10.3390/nu12041125
[13]	李云亮, 刘晓霜, 徐雅宣, 等. 乳清蛋白在临床肠内营养中的应用进展[J]. 中国乳品工业,2023,51(7):45−51. [LI Y L, LIU X S, XU Y X, et al. Progress in the application of whey protein in clinical enteral nutrition[J]. China Dairy Industry,2023,51(7):45−51.] LI Y L, LIU X S, XU Y X, et al. Progress in the application of whey protein in clinical enteral nutrition[J]. China Dairy Industry, 2023, 51（7）: 45−51.
[14]	蔡东联, 曹翔, 李燕, 等. 大豆蛋白抗辐射对肠屏障的损害[J]. 中国临床营养杂志,2001,9(3):16−19. [CAI D L, CAO X, LI Y, et al. Alleviating radiation damage and protecting the bowel barrier functio n in SD rat by using the soybean protein[J]. Chinese Journal of Clinical Nutrition,2001,9(3):16−19.] CAI D L, CAO X, LI Y, et al. Alleviating radiation damage and protecting the bowel barrier functio n in SD rat by using the soybean protein[J]. Chinese Journal of Clinical Nutrition, 2001, 9（3）: 16−19.
[15]	蒲玲玲, 郭长江. 乳清蛋白的组成及其主要保健功能[J]. 中国食物与营养,2011,17(6):68−70. [PU L L, GUO C J. Composition of whey protein and its main health care function[J]. Food and Nutrition in China,2011,17(6):68−70.] PU L L, GUO C J. Composition of whey protein and its main health care function[J]. Food and Nutrition in China, 2011, 17（6）: 68−70.
[16]	ZHAO X, XU X X, LIU Y, et al. The in vitro protective role of bovine lactoferrin on intestinal epithelial barrier[J]. Molecules,2019,24(1):148. doi: 10.3390/molecules24010148
[17]	张亮. 大豆异黄酮降脂作用及其机制研究[D]. 延边:延边大学, 2014. [ZHANG L. Study on lipid-lowering effect of soybean isoflavone and its mechanism[D]. Yanbian:Yanbian University, 2014.] ZHANG L. Study on lipid-lowering effect of soybean isoflavone and its mechanism[D]. Yanbian: Yanbian University, 2014.
[18]	张翠芬. 大豆异黄酮对心血管疾病的研究综述[J]. 中国食品添加剂,2018(9):210−213. [ZHANG C F. Research review of soybean isoflavones on cardiovascular diseases[J]. China Food Additives,2018(9):210−213.] ZHANG C F. Research review of soybean isoflavones on cardiovascular diseases[J]. China Food Additives, 2018（9）: 210−213.
[19]	梁得福, 孟维洪, 舒欣, 等. 黑豆-乳清双蛋白膳食对大鼠体内免疫的调节作用[J]. 现代食品科技, 2024, 40(1):1−9. [LIANG D F, MENG W H, SHU X, et al. Regulatory effect of black bean-whey double protein diet on immunity in rats[J]. Modern Food Science & Technology, 2021, 40(1):1−9.] LIANG D F, MENG W H, SHU X, et al. Regulatory effect of black bean-whey double protein diet on immunity in rats[J]. Modern Food Science & Technology, 2021, 40（1）: 1−9.
[20]	ZHUANG K J, MENG W H, SHU X, et al. Fecal metabonomics combined with 16S rDNA sequencing to analyze the changes of gut microbiota in rats fed with different protein source diets[J]. European Journal of Nutrition,2023,62(6):2687−2703. doi: 10.1007/s00394-023-03168-y
[21]	杨轶仑, 路晓光. 肠道菌群与肠黏膜机械屏障关系研究进展[J]. 内蒙古医学杂志,2019,51(10):1195−1197. [YANG Y L, LU X G. Research progress on the relationship between intestinal flora and intestinal mucosal mechanical barrier[J]. Inner Mongolia Medical Journal,2019,51(10):1195−1197.] YANG Y L, LU X G. Research progress on the relationship between intestinal flora and intestinal mucosal mechanical barrier[J]. Inner Mongolia Medical Journal, 2019, 51（10）: 1195−1197.
[22]	杨宇. β-胡萝卜素对脂多糖诱导的大鼠肠道炎症反应的调节作用及分子机理研究[D]. 长春:吉林农业大学, 2021. [YANG Y. Regulation of β-carotene on the rat intestinal inflammation induced by lipopolysaccharide and its molecular mechanisms[D]. Changchun:Jilin Agricultural University, 2021.] YANG Y. Regulation of β-carotene on the rat intestinal inflammation induced by lipopolysaccharide and its molecular mechanisms[D]. Changchun: Jilin Agricultural University, 2021.
[23]	赵伟, 孙国志. 不同种实验动物间用药量换算[J]. 畜牧兽医科技信息,2010(5):52−53. [ZHAO W, SUN G Z. Conversion of dosage between different experimental animals[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine,2010(5):52−53.] ZHAO W, SUN G Z. Conversion of dosage between different experimental animals[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2010（5）: 52−53.
[24]	周荣荣, 庄柯瑾, 许庆鹏, 等. 优质豆类蛋白筛选及其与乳清蛋白最佳复配比例的研究[J]. 中国粮油学报,2022,37(5):93−101. [ZHOU R R, ZHUANG K J, XU Q P, et al. Study on screening of high-quality legume protein and the optimum mixing ratio with whey protein[J]. Journal of the Chinese Cereals and Oils Association,2022,37(5):93−101.] ZHOU R R, ZHUANG K J, XU Q P, et al. Study on screening of high-quality legume protein and the optimum mixing ratio with whey protein[J]. Journal of the Chinese Cereals and Oils Association, 2022, 37（5）: 93−101.
[25]	AZKANAZ M, COROMINAS-MURTRA B, ELLENBROEK T J S, et al. Retrograde movements determine effective stem cell numbers in the intestine[J]. Nature,2022,607(Jul.21):548−554.
[26]	WANG Z, QU Y J, CUI M. Modulation of stem cell fate in intestinal homeostasis, injury and repair.[J]. World Journal of Stem Cells,2023,15(5):354−368. doi: 10.4252/wjsc.v15.i5.354
[27]	王涛, 季珊珊, 汤鑫磊, 等. 大豆和猪肉来源的高蛋白饮食对肥胖小鼠的干预作用及肠道菌群的影响[J/OL]. 食品科学技术学报:1−13[2024-11-05]. DOI:10.1151.ts.20240125.1932.002. [WANG T, JI S S, TANG X L, et al. Intervention and intestinal microflora effects of soybean and pork high-protein diets on obese mice[J/OL]. Journal of Food Science and Technology:1−13[2024-11-05]. DOI:10.1151.ts.20240125.1932.002.html.] WANG T, JI S S, TANG X L, et al. Intervention and intestinal microflora effects of soybean and pork high-protein diets on obese mice[J/OL]. Journal of Food Science and Technology: 1−13[2024-11-05]. DOI: 10.1151.ts.20240125.1932.002.html.
[28]	ZHANG Z, LU W J, LIU P F, et al. Microbial modifications with Lycium barbarum L. oligosaccharides decrease hepatic fibrosis and mitochondrial abnormalities in mice[J]. Phytomedicine:International Journal of Phytotherapy and Phytopharmacology,2023,120:155068. doi: 10.1016/j.phymed.2023.155068
[29]	LI C G, SUN Y N, HE T C, et al. Synergistic effect of lactoferrin and osteopontin on intestinal barrier injury[J]. International Journal of Biological Macromolecules,2023,253(P7):127416−127416.
[30]	胡光源, 赵峰, 张宏福, 等. 饲粮蛋白质来源与水平对生长猪空肠液组成的影响[J]. 动物营养学报,2010,22(5):1220−1225. [HU G Y, ZHAO F, ZHANG H F, et al. Effects of the source and level of dietary protein on the composition of jejunal fluid in growing pigs[J]. Chinese Journal of Animal Nutrition,2010,22(5):1220−1225.] HU G Y, ZHAO F, ZHANG H F, et al. Effects of the source and level of dietary protein on the composition of jejunal fluid in growing pigs[J]. Chinese Journal of Animal Nutrition, 2010, 22（5）: 1220−1225.
[31]	GAO J, YIN J, XU K, et al. Protein level and infantile diarrhea in a postweaning piglet model[J]. Mediators of Inflammation,2020,2020:1937387.
[32]	BEUTEL O, MARASPINI R, POMBO-GARCIA K, et al. Phase separation of zonula occludens proteins drives formation of tight junctions[J]. Cell,2019,179(4):923−936. doi: 10.1016/j.cell.2019.10.011
[33]	ARRANZ E, SEGAT A, VELAYOS G, et al. Dairy and plant based protein beverages:In vitro digestion behaviour and effect on intestinal barrier biomarkers[J]. Food Res Int, 2023, 169:112815.
[34]	DIAS C M, COISNE C, LAZAREVIC I, et al. Publisher correction:claudin-3-deficient C57BL/6J mice display intact brain barriers[J]. Scientific Reports,2019,9(1):1. doi: 10.1038/s41598-018-37186-2
[35]	SOUZA D F W, FORTUNATO-MIRANDA N, ROBBS K B, et al. Claudin-3 overexpression increases the malignant potential of colorectal cancer cells:roles of ERK1/2 and PI3K-Akt as modulators of EGFR signaling[J]. PLoS ONE,2017,8(9):e74994.
[36]	TANAKA H, IMASATO M, YAMAZAKI Y, et al. Claudin-3 regulates bile canalicular paracellular barrier and cholesterol gallstone core formation in mice[J]. Journal of Hepatology:The Journal of the European Association for the Study of the Liver,2018,69(6):1308−1316.
[37]	邢晓辉, 李力仙, 郭天林, 等. Occludin蛋白与细胞间紧密连接关系及其临床意义[J]. 现代生物医学进展,2015,15(8):1553−1555,1405. [XING X H, LI L X, GUO T L, et al. Relationship between Occludin protein and tight junction between cells and its clinical significance[J]. Progress in Modern Biomedicine,2015,15(8):1553−1555,1405.] XING X H, LI L X, GUO T L, et al. Relationship between Occludin protein and tight junction between cells and its clinical significance[J]. Progress in Modern Biomedicine, 2015, 15（8）: 1553−1555,1405.
[38]	BEUTHEU S, GHOUZALI I, GALAS L, et al. Glutamine and arginine improve permeability and tight junction protein expression in methotrexate-treated Caco-2 cells[J]. Clin Nutr,2013,32(5):863−869. doi: 10.1016/j.clnu.2013.01.014
[39]	AMEHO C K, ADJEI A A, HARRISON E K, et al. Prophylactic effect of dietary glutamine supplementation on interleukin 8 and tumour necrosis factor alpha production in trinitrobenzene sulphonic acid induced colitis[J]. Gut,1997,41(4):487−493. doi: 10.1136/gut.41.4.487
[40]	胡天念. 罗伊氏乳杆菌SBC5-3对肠上皮细胞的免疫调节机制研究[D]. 昆明:云南农业大学, 2023. [HU T N. Study on Immunomodulatory mechanism of Lactobacillus reuteri SBC5-3 on intestinal epithelial cells[D]. Kunming:Yunnan Agricultural University, 2023.] HU T N. Study on Immunomodulatory mechanism of Lactobacillus reuteri SBC5-3 on intestinal epithelial cells[D]. Kunming: Yunnan Agricultural University, 2023.
[41]	吴欢听. 甘氨酸对脂多糖刺激的仔猪肠道损伤及肌肉蛋白质合成和降解的调控作用[D]. 武汉:武汉轻工大学, 2015. [WU H T. Regulative effect of glycine on intestinal injury and muscle protein sythesis and degradation of piglets after lipopolysaccharide challenge[D]. Wuhan:Wuhan Polytechnic University, 2015.] WU H T. Regulative effect of glycine on intestinal injury and muscle protein sythesis and degradation of piglets after lipopolysaccharide challenge[D]. Wuhan: Wuhan Polytechnic University, 2015.
[42]	LENG W B, LIU Y L, SHI H F, et al. Aspartate alleviates liver injury and regulates mRNA expressions of TLR4 and NOD signaling-related genes in weaned pigs after lipopolysaccharide challenge[J]. The Journal of Nutritional Biochemistry,2014,25(6):592−599. doi: 10.1016/j.jnutbio.2014.01.010

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(4)

1.	陆源添，刘迪. 杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析. 食品工业科技. 2025(02): 208-217 . 本站查看
2.	郭世纪，刘政，王会. 苹果多酚的提取工艺优化. 食品工业. 2024(07): 37-43 .
3.	罗诗茵，聂菁，韩文敏，林丹丹，廖兰，曾新安. 脉冲电场回收农产品加工废料中生物活性物质的研究进展. 食品工业科技. 2024(19): 392-398 . 本站查看
4.	时文盼，郭嘉欣，蔺永刚，柴雨阳，郭玉锦，武斌，王俊龙. 弯萼金丝桃总多酚提取工艺优化及抗氧化、降糖活性研究. 食品与发酵工业. 2024(21): 206-215 .