Effect of Ultrasonic Combined with Ethanol Modification on the Structural and Functional Properties of Porcine Heart Protein

LU Jinming; SHANG Yongbiao; HOU Dajun; CAI Qiuxing

doi:10.13386/j.issn1002-0306.2024010052

Volume 45 Issue 17

Aug. 2024

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Science and Technology of Food Industry > 2024 > 45(17): 30-39. > DOI: 10.13386/j.issn1002-0306.2024010052

LU Jinming, SHANG Yongbiao, HOU Dajun, et al. Effect of Ultrasonic Combined with Ethanol Modification on the Structural and Functional Properties of Porcine Heart Protein[J]. Science and Technology of Food Industry, 2024, 45(17): 30−39. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024010052.

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Effect of Ultrasonic Combined with Ethanol Modification on the Structural and Functional Properties of Porcine Heart Protein

LU Jinming^{1, 2,},
SHANG Yongbiao³,
HOU Dajun³,
CAI Qiuxing^{1, 2, ,}

1.
College of Food Engineering, Beibu Gulf University, Qinzhou 535011, China
2.
Guangxi College and University Key Laboratory of High-value Utilization of Seafood and Prepared Food in Beibu Gulf, Qinzhou 535011, China
3.
College of Food Science, Southwest University, Chongqing 400715, China

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Received Date: January 07, 2024
Available Online: June 30, 2024

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Abstract

Abstract

In order to improve the functional properties of porcine heart protein and improve its application feasibility in the food industry, this study modified porcine heart protein (PHP) by ultrasonic (frequency of 20 kHz, power of 350 W, treatment for 8 min, working 5 s with 2 s intervals) combined with different concentrations of ethanol (0~80%). The changes of molecular structure, microstructure, active sulfhydryl content, surface hydrophobicity, solubility, emulsification, emulsion particle size and emulsion Zeta potential of modified PHP were determined. The results showed that the primary structure, secondary structure and microstructure of PHP were significantly changed under the combined action of ultrasonic and 40% ethanol modification. The ultraviolet absorption intensity of PHP was the largest, the active sulfhydryl content and surface hydrophobicity were the largest. The solubility of protein was significantly reduced (P<0.05), the emulsifying activity and emulsifying stability were the best, the particle size of protein emulsion was the smallest, and the absolute value of Zeta potential was the largest. Considering the improvement effect of PHP emulsification characteristics, ultrasonic combined with 40% ethanol modification treatment is the best modification condition for PHP.
- ultrasonic,
- ethanol,
- porcine heart protein (PHP) modification,
- protein structure,
- protein functional properties

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References

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