Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H<sub>2</sub>O<sub>2</sub> Oxidative Damage in L929 Cells

WU Qici; LIN Zhichao; PAN Xiaoming; XUE Yu; HUANG Jiafu; PAN Yutian

doi:10.13386/j.issn1002-0306.2023120072

Volume 45 Issue 13

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(13): 204-211. > DOI: 10.13386/j.issn1002-0306.2023120072

WU Qici, LIN Zhichao, PAN Xiaoming, et al. Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H₂O₂ Oxidative Damage in L929 Cells[J]. Science and Technology of Food Industry, 2024, 45(13): 204−211. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120072.

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Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H₂O₂ Oxidative Damage in L929 Cells

1.
Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou 363000, China
2.
Mendel (Xiamen) Biotechnology Co., Ltd., Xiamen 361000, China

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Received Date: December 10, 2023
Available Online: April 26, 2024

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Abstract

Abstract

Objective: The extraction process of alkali-extracted abalone viscera polysaccharides (Aavp) was optimized, and the preventive and repair effects of purified Aavp on H₂O₂ oxidative damage on L929 cells were studied to provide a reference for the development and application of Aavps. Methods: Crude Aavp was obtained by hot alkali extraction followed by alcohol precipitation, and its extraction process was optimized by the response surface method. The preventive and repair abilities of H₂O₂ oxidative damage on L929 cells were analyzed. Resulst: The optimum extraction conditions of crude Aavps were as follows: Solid-liquid ratio of 1:150 g/mL, extraction time of 2 h, and extraction temperature of 70 ℃. Under these optimal conditions, the yield of crude Aavp was 8.57%. In the prevention test of H₂O₂ oxidative damage on L929 cells, different doses of purified Aavp (20, 50 and 100 μg/mL) had a significant preventive effect on L929 cell oxidative damage (P<0.001) but did not show a dose effect. The cell survival rate of the middle dose group (50 μg/mL) was the highest (71.94%±3.08%), which was slightly better than the positive control vitamin E group but not significant. In the repair test, different doses of purified Aavp (20, 50 and 100 μg/mL) had a significant repair effect on the oxidative damage of L929 cells (P<0.001) and showed a dose effect. In contrast, the cell survival rate of the high-dose group (100 μg/mL) was the highest (90.93%±1.17%), slightly higher than that of the positive control vitamin E group (87.96%±3.05%). The results showed that purified Aavp showed an excellent preventive and repair effect on H₂O₂ oxidative damage in L929 cells. Conclusion: Aavp has an ability to repair H₂O₂ oxidative damage of L929 cells.
- abalone viscera,
- alkali-extracted polysaccharide,
- response surface optimization,
- oxidative damage

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Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H₂O₂ Oxidative Damage in L929 Cells