Fermentation Conditions Optimization and Components Analysis of Antimicrobial Substances-producing Endophytic Fungus CWJ01 from <i>Acanthopanax senticosus</i>

LI Yuguo; JIANG Kun; ZHANG Daozhi; XU Hengwei; FENG Danping; MENG Xiaoqian; ZHENG Chunying

doi:10.13386/j.issn1002-0306.2023110328

Volume 45 Issue 17

Aug. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(17): 209-220. > DOI: 10.13386/j.issn1002-0306.2023110328

LI Yuguo, JIANG Kun, ZHANG Daozhi, et al. Fermentation Conditions Optimization and Components Analysis of Antimicrobial Substances-producing Endophytic Fungus CWJ01 from Acanthopanax senticosus[J]. Science and Technology of Food Industry, 2024, 45(17): 209−220. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110328.

Citation:

PDF (4080 KB)

Fermentation Conditions Optimization and Components Analysis of Antimicrobial Substances-producing Endophytic Fungus CWJ01 from Acanthopanax senticosus

1.
Heilongjiang Fangzheng Forestry Bureau Co., Ltd., Harbin 150822, China
2.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China

More Information

Received Date: November 29, 2023
Available Online: July 07, 2024

Graphical Abstract

Abstract

Abstract

An endophytic fungus CWJ01 with good antibacterial activity was isolated from the leaves of Acanthopanax senticosus. In order to improve the antibacterial activity of the fermentation broth of this strain and explore the substance basis of its antibacterial activity, the fermentation conditions of the endophytic fungus CWJ01 were optimized by orthogonal experiment according the antibacterial activity on the basis of single factor experiments. The secondary metabolites contained in the endophytic fungus CWJ01 were separated by column chromatography. The strains of the endophytic fungus CWJ01 were identified by morphology and ITS sequence analysis. The results showed that the optimum fermentation conditions of CWJ01 were as follows: PDB as the base medium, 2% glucose as the carbon source, 1% peptone as the nitrogen source. The initial pH of the medium was 7.0, the liquid content of the medium was 60%, the inoculated strain was 5%, the rotating speed was 140 r/min, the temperature was 28 ℃, and the culture time was 15 days. Under the optimal process conditions, the diameter of inhibition zone of CWJ01 fermentation broth against Staphylococcus aureus was 27.48±0.17 mm. Trans-cinnamic acid and benzoic acid were isolated from the fermentation broth of endogenous fungus CWJ01, and the endophytic fungus CWJ01 was identified as Penicillium polonicum. This study can provide reference for further exploration of antibacterial active substances of endophytes.
- Acanthopanax senticosus,
- endophytic fungus,
- bacteriostatic activity,
- fermentation condition,
- strain identification,
- secondary metabolites

FullText(HTML)

References (34)

References

[1]	国家药典委员会. 中华人民共和国药典:一部[M]. 北京:中国医药科技出版社, 2020:125. [National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China:A [M]. Beijing:China Medical Science and Technology Press, 2020:125.] National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China: A [M]. Beijing: China Medical Science and Technology Press, 2020: 125.
[2]	LIANG C, XIU L X, HUI F, et al. Isolation and identificationn of an-thocuanin component in the fruits of Acanthopanax sessiliflorus (Rupr.&Maxim) Seem. By means of high speed counter current chromatography and evaluation of its antioxidant activity[J]. Molecules,2020,25(8):20−34.
[3]	CHEN X H, ZHAO Y Y, WANG Q, et al. Chemical constituents from the stems of Acanthopanax senticosus with their cytotoxic activities[J]. Asian Natural Products Research,2022,24(7):673−678. doi: 10.1080/10286020.2021.1951713
[4]	LI J, LI N, LEE H S, et al. Four new sesqui-lignans isolated from Acanthopanax senticosus and their diacylglycerol acyltransferase (DGAT) inhibitory activity[J]. Fitoterapia,2016,109:185−189. doi: 10.1016/j.fitote.2016.01.009
[5]	XIA Y G, HUANG Y X, LIANG J, et al. Comparable studies of two polysaccharides from leaves of Acanthopanax senticosus:Structure and antioxidation[J]. International Journal of Biological Macromolecules,2020,147:350−362. doi: 10.1016/j.ijbiomac.2019.12.244
[6]	李强, 张若冰, 杨玉赫, 等. 刺五加叶化学成分及药理作用研究进展[J]. 药学研究,2023,42(7):495−501. [LI Qiang, ZHANG Ruobing, YANG Yuhe, et al. Research progress on chemical constituents and pharmacological effects of Acanthopanax senticosus leaves[J]. Journal of Pharmaceutical Research,2023,42(7):495−501.] LI Qiang, ZHANG Ruobing, YANG Yuhe, et al. Research progress on chemical constituents and pharmacological effects of Acanthopanax senticosus leaves[J]. Journal of Pharmaceutical Research, 2023, 42（7）: 495−501.
[7]	ZHANG Xiaoxu, ZHANG Qi , YU Menghan, et al. Integrating serum pharmacochemistry and network pharmacology to explore the molecular mechanisms of Acanthopanax senticosus (Rupr. & Maxim.) Harms on attenuating doxorubicin-induced myocardial injury[J]. Journal of Ethnopharmacology, 2024, 319(3):117349.
[8]	RUI S, ZHAO Y, WANG J Y, et al. Sedative and hypnotic effects of ethanol extracts of Acanthopanax senticosus root bark and Acanthopanax sessiliflorus Brak and their mechanisms[J]. Journal of Jilin University(Medicine Edition),2020,46(5):917−924.
[9]	JIN L, SCHMIECH M, GAAFARY M E, et al. A comparative study on root and bark extracts of Eleutherococcus senticosus and their effects on human macrophages[J]. Phytomedicine,2020,68:153181. doi: 10.1016/j.phymed.2020.153181
[10]	TETSUO S, MIYAKO N, MASAFUMI S, et al. The fruit of Acanthopanax senticosus (Rupr. et Maxim.) harms improves insulin resistance and hepatic lipid accumulation by modulation of liver adenosine monophosphate activated protein kinase activity and lipogenic gene expression in high- fat dietfed obese mice[J]. Nutrition Research,2016,36(10):98−102.
[11]	崔立东. 刺五加何以成为“寒地龙药”之首[N]. 黑龙江日报, 2023-07-31(006). [CUI Lidong. Why Acanthopanax sessiliflorus became the first of "Cold Earth Dragon Medicine"[N]. Heilongjiang Daily, 2023-07-31(006).] CUI Lidong. Why Acanthopanax sessiliflorus became the first of "Cold Earth Dragon Medicine"[N]. Heilongjiang Daily, 2023-07-31（006）.
[12]	卓长清, 赵欣, 栾朝霞. 枸杞刺五加运动饮料研制及抗疲劳作用研究[J]. 中国食品添加剂,2019,30(12):131−136. [ZHUO C Q, ZHAO X, LUAN Z X. Study on preparation and anti-fatiguefunction of wolfberry and Acanthopanax senticosus sports beverage[J]. China Food Additives,2019,30(12):131−136.] ZHUO C Q, ZHAO X, LUAN Z X. Study on preparation and anti-fatiguefunction of wolfberry and Acanthopanax senticosus sports beverage[J]. China Food Additives, 2019, 30（12）: 131−136.
[13]	CHEN X Q, JIA X D, YANG S, et al. Optimization of ultrasonic-assisted extraction of flavonoids, polysaccharides, and eleutherosides from Acanthopanax senticosus using response surface methodology in development of health wine[J]. LWT,2022,165:113725,. doi: 10.1016/j.lwt.2022.113725
[14]	谭龙, 曹贵阳, 郭盛磊, 等. 2021年黑龙江省中药材生产统计数据分析[J]. 中国现代中药,2023,25(11):2296−2300. [TAN Long, CAO Guiyang, GUO Shenglei, et al. Analysis of production statistics of Chinese medicinal materials in Heilongjiang Province in 2021[J]. Modern Chinese Medicine,2023,25(11):2296−2300.] TAN Long, CAO Guiyang, GUO Shenglei, et al. Analysis of production statistics of Chinese medicinal materials in Heilongjiang Province in 2021[J]. Modern Chinese Medicine, 2023, 25（11）: 2296−2300.
[15]	谈文权. 不同设施栽培方式对刺五加采摘期及产量的影响[J]. 中国果菜,2020,40(7):72−75. [TAN W Q. Effects of different cultivation methods on picking time and yield of Acanthopanax senticosus[J]. Chinese Fruit and Vegetable,2020,40(7):72−75.] TAN W Q. Effects of different cultivation methods on picking time and yield of Acanthopanax senticosus[J]. Chinese Fruit and Vegetable, 2020, 40（7）: 72−75.
[16]	CAO J, LIU B, XU X, et al. Plant endophytic fungus extract ZNC improved potato immunity, yield, and quality[J]. Front Plant Sci,2021,12:707256.
[17]	SONIA W, ADIL A B, ABRAR A K, et al. Endophytic fungi:Unravelling plant-endophyte interaction and the multifaceted role of fungal endophytes in stress amelioration[J]. Plant Physiology and Biochemistry,2023,108−174.
[18]	闫佳佳, 万璐, 尤梦瑶, 等. 产香兰素内生真菌GP20的鉴定及发酵条件研究[J]. 中国调味品,2022,47(11):44−49. [YAN Jiajia, WAN Lu, YOU Mengyao, et al. Identification and fermentation conditions of vanillin-producing endophytic fungus GP20[J]. Chinese Condiment,2022,47(11):44−49.] YAN Jiajia, WAN Lu, YOU Mengyao, et al. Identification and fermentation conditions of vanillin-producing endophytic fungus GP20[J]. Chinese Condiment, 2022, 47（11）: 44−49.
[19]	尤梦瑶, 闫佳佳, 万璐, 等. 黄芪内生枯草芽孢杆菌HS8次生代谢产物研究[J]. 黑龙江大学自然科学学报,2022,39(6):679−685. [YOU Mengyao, YAN Jiajia, WAN Lu, et al. Study on secondary metabolites of endophytic Bacillus subtilis HS8[J]. Natural Science Journal of Heilongjiang University,2022,39(6):679−685.] YOU Mengyao, YAN Jiajia, WAN Lu, et al. Study on secondary metabolites of endophytic Bacillus subtilis HS8[J]. Natural Science Journal of Heilongjiang University, 2022, 39（6）: 679−685.
[20]	WANG Fuqi, HU Tianshuai, HAN Yangyang, et al. Biological control of gentian spot blight by the combination of endophytic fungi fermentation broth and traditional Chinese medicine extractions[J]. Physiological and Molecular Plant Pathology,2023,128:102149.
[21]	PRINCE H E, ANDERSON W L, TOMASI T B. Inhibition of L-cell growth in agarose (ILGA):A simple inexpensive method for the detection and quantitation of factors inhibiting tumor cell growth[J]. Journal of Immunological Methods,1982,48(3):367−372.
[22]	李俊秀, 廖夏云, 赵立春, 等. HPLC测定青钱柳叶片中槲皮素、山奈酚的含量[J]. 湖北农业科学,2023,62(10):166−172. [LI Junxiu, LIAO Xiayun, ZHAO Lichun, et al. Determination of quercetin and kaempferol in leaves of Salix cyclocarya by HPLC[J]. Hubei Agricultural Sciences,2023,62(10):166−172.] LI Junxiu, LIAO Xiayun, ZHAO Lichun, et al. Determination of quercetin and kaempferol in leaves of Salix cyclocarya by HPLC[J]. Hubei Agricultural Sciences, 2023, 62（10）: 166−172.
[23]	魏宝红, 刘佳, 毕旺华, 等. 药用真菌桑黄的液态发酵工艺优化及体外抗肿瘤活性研究[J]. 生物化工,2023,9(4):19−25. [WEI Baohong, LIU Jia, BI Wanghua, et al. Optimization of liquid fermentation process and in vitro antitumor activity of the medicinal fungus Sanghuangporus spp doi: 10.3969/j.issn.2096-0387.2023.04.005 J]. Biochemistry,2023,9(4):19−25. doi: 10.3969/j.issn.2096-0387.2023.04.005
[24]	董玲, 张凤菊, 赵驰, 等. 基于UPLC-QTOF-MS解析苦荞黄酮组成及药理学网络分析[J]. 核农学报,2024,05(38):911−918. [DONG Ling, ZHANG Fengju, ZHAO Chi, et al. Composition and pharmacologic network analysis of flavonoids from Tartary buckwheat based on UPLC-QTOF-MS[J]. Chinese Journal of Nuclear Agriculture,2024,05(38):911−918.] doi: 10.11869/j.issn.1000-8551.2024.05.0911 DONG Ling, ZHANG Fengju, ZHAO Chi, et al. Composition and pharmacologic network analysis of flavonoids from Tartary buckwheat based on UPLC-QTOF-MS[J]. Chinese Journal of Nuclear Agriculture, 2024, 05（38）: 911−918. doi: 10.11869/j.issn.1000-8551.2024.05.0911
[25]	徐荧, 马成业, 张琛, 等. 液体核磁共振技术用于木质素结构表征的研究进展[J]. 中国造纸学报,2024,39(2):23−34. [XU Y, MA Y C, ZHANG C, et al. Research progress on solution-state nuclear magnetic resonance (NMR) technique for lignin structure characterization[J]. Chinese Paper Journal,2024,39(2):23−34.] XU Y, MA Y C, ZHANG C, et al. Research progress on solution-state nuclear magnetic resonance (NMR) technique for lignin structure characterization[J]. Chinese Paper Journal, 2024, 39（2）: 23−34.
[26]	邵力平, 沈瑞祥, 张素轩, 等. 真菌分类学[M]. 北京:中国林业出版社, 1984. [SHAO Liping, SHEN Ruixiang, ZHANG Suxuan, et al. Taxonomy of fungi [M]. Beijing:China Forestry Publishing House, 1984.] SHAO Liping, SHEN Ruixiang, ZHANG Suxuan, et al. Taxonomy of fungi [M]. Beijing: China Forestry Publishing House, 1984.
[27]	魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社, 1982. [WEI Jingchao. Fungal identification manual [M]. Shanghai:Shanghai Science and Technology Press, 1982.] WEI Jingchao. Fungal identification manual [M]. Shanghai: Shanghai Science and Technology Press, 1982.
[28]	万璐, 闫佳佳, 尤梦瑶, 等. 产甘草次酸内生真菌GRE111的鉴定及发酵条件研究[J]. 中国调味品,2022,47(10):41−46. [WAN Lu, YAN Jiajia, YOU Mengyao, et al. Identification and fermentation conditions of glycyrrhetinic acid-producing endophytic fungus GRE111[J]. Chinese Condiments,2022,47(10):41−46.] doi: 10.3969/j.issn.1000-9973.2022.10.008 WAN Lu, YAN Jiajia, YOU Mengyao, et al. Identification and fermentation conditions of glycyrrhetinic acid-producing endophytic fungus GRE111[J]. Chinese Condiments, 2022, 47（10）: 41−46. doi: 10.3969/j.issn.1000-9973.2022.10.008
[29]	张赫, 尤梦瑶, 万璐, 等. 产紫丁香苷内生真菌CJ7的鉴定及发酵条件研究[J]. 中国农学通报,2022,38(25):143−150. [ZHANG He, YOU Mengyao, WAN Lu, et al. Identification and fermentation conditions of endophytic fungus CJ7 producing syringin[J]. Chinese Agricultural Science Bulletin,2022,38(25):143−150.] doi: 10.11924/j.issn.1000-6850.casb2021-0888 ZHANG He, YOU Mengyao, WAN Lu, et al. Identification and fermentation conditions of endophytic fungus CJ7 producing syringin[J]. Chinese Agricultural Science Bulletin, 2022, 38（25）: 143−150. doi: 10.11924/j.issn.1000-6850.casb2021-0888
[30]	ABDEL-FATAH S, EL-BATAL A, EL-SHERBINY G, et al. Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba[J]. Biotechnology Reports,2021,30:e00623. doi: 10.1016/j.btre.2021.e00623
[31]	ZHANG Shuang, GUO Yu, ZHAO Qianhui, et al. Study on the bacteriostatic action of Chinese herbal medicine on avian Trichosporon[J]. Poultry Science,2020,99(9):4530−4538. doi: 10.1016/j.psj.2020.06.011
[32]	赵俊俊, 马瑞霞. 杏鲍菇液体菌种摇瓶发酵条件的研究[J]. 中国园艺文摘,2017,33(8):27−28,83. [ZHAO Junjun, MA Ruixia. Study on fermentation conditions of liquid strains of Pleurocenti eryngii[J]. Chinese Horticultural Abstracting,2017,33(8):27−28,83.] doi: 10.3969/j.issn.1672-0873.2017.08.010 ZHAO Junjun, MA Ruixia. Study on fermentation conditions of liquid strains of Pleurocenti eryngii[J]. Chinese Horticultural Abstracting, 2017, 33（8）: 27−28,83. doi: 10.3969/j.issn.1672-0873.2017.08.010
[33]	张赫, 关昕, 祁可香, 等. 产反式肉桂酸内生真菌GRE4的鉴定及发酵条件研究[J]. 中国调味品,2023,48(7):25−31. [ZHANG He, GUAN Xin, QI Kexiang, et al. Identification and fermentation conditions of trans-cinnamic acid-producing endophyte GRE4[J]. Chinese Condiment,2023,48(7):25−31.] doi: 10.3969/j.issn.1000-9973.2023.07.005 ZHANG He, GUAN Xin, QI Kexiang, et al. Identification and fermentation conditions of trans-cinnamic acid-producing endophyte GRE4[J]. Chinese Condiment, 2023, 48（7）: 25−31. doi: 10.3969/j.issn.1000-9973.2023.07.005
[34]	WANG Dandan, SUN Li, YU Hong, et al. Whole-genome analysis of the benzoic acid-degrading bacterium Bacillus halotolerans B28 to reveal its phytoprobiotic effects[J]. International Biodeterioration & Biodegradation,2023,185:105668.

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(2)

1.	陈璐瑶，张志刚，邢国良，王捷，姚玉军，吕兆林. 沙棘叶及其醇提冻干粉挥发性物质和关键营养成分分析. 食品工业科技. 2025(08): 272-280 . 本站查看
2.	宋娟，康三江，张海燕，曾朝珍，袁晶，慕钰文，苟丽娜. 响应面法优化黑苹果发酵工艺及其抗氧化活性评价. 中国酿造. 2024(11): 159-166 .