Optimization of Ultrasonic-assisted Extraction of Polyphenols from <i>Taraxacum kok-saghyz</i> Rodin Roots and Its Antioxidant, Hypoglycemic Activity <i>in Vitro</i>

ZHANG Na; LIU Li; LI Lu; LÜ Jingjing; DONG Yiyang

doi:10.13386/j.issn1002-0306.2023100144

Volume 45 Issue 17

Aug. 2024

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Science and Technology of Food Industry > 2024 > 45(17): 200-208. > DOI: 10.13386/j.issn1002-0306.2023100144

ZHANG Na, LIU Li, LI Lu, et al. Optimization of Ultrasonic-assisted Extraction of Polyphenols from Taraxacum kok-saghyz Rodin Roots and Its Antioxidant, Hypoglycemic Activity in Vitro[J]. Science and Technology of Food Industry, 2024, 45(17): 200−208. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100144.

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Optimization of Ultrasonic-assisted Extraction of Polyphenols from Taraxacum kok-saghyz Rodin Roots and Its Antioxidant, Hypoglycemic Activity in Vitro

ZHANG Na^{1, 2,},
LIU Li³,
LI Lu¹,
LÜ Jingjing¹,
DONG Yiyang^2, ,

1.
Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, College of Food Science and Engineering, Tarim University, Alar 843300, China
2.
Rubber Plant Research Center, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
3.
The Center of Inspection and Test on Produce Quality in Hunan Province, Changsha 410005, China

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Received Date: October 18, 2023
Available Online: July 04, 2024

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Abstract

Abstract

Using Taraxacum kok-saghyz Rodin (TKS) roots as the raw material, the liquid-solid ratio, ultrasonic power, extraction temperature, and extraction time were selected as experimental factors. On the basis of single factor experiments, the response surface methodology was used to optimize the ultrasonic-assisted extraction process of polyphenols from the TKS roots. The in vitro antioxidant and hypoglycemic activity of the polyphenols extracted from TKS roots were measured. The results showed that the optimal extraction conditions were liquid-solid ratio of 33:1 mL/g, ultrasonic power of 270 W, extraction temperature of 61 ℃, and extraction time of 31 min. Under these conditions, the yield of polyphenols from TKS roots was 1.46%±0.03%. The in vitro activity test showed that the half inhibitory concentrations (IC₅₀) of polyphenol extracts on DPPH and ABTS⁺ radicals were 54.06 and 39.27 µg/mL, respectively, the IC₅₀ values for α-amylase and α-glucosidase were 133.26 and 50.22 µg/mL, respectively. This indicated that the polyphenols from TKS roots had strong antioxidant and hypoglycemic activities in vitro. This study can provide theoretical basis for the comprehensive development and utilization of TKS.
- Taraxacum kok-saghyz Rodin roots,
- polyphenols,
- extraction,
- response surface methodology,
- antioxidant,
- hypoglycemic activity

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References

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