Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from <i>Mentha haplocalyx</i> Briq. with Subcritical Water

ZHANG Qingqing; ZHANG Mengjiao; MA Yiming; CAI Xueyi

doi:10.13386/j.issn1002-0306.2023100088

Volume 45 Issue 10

May 2024

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Science and Technology of Food Industry > 2024 > 45(10): 17-24. > DOI: 10.13386/j.issn1002-0306.2023100088

ZHANG Qingqing, ZHANG Mengjiao, MA Yiming, et al. Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq. with Subcritical Water[J]. Science and Technology of Food Industry, 2024, 45(10): 17−24. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100088.

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Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq. with Subcritical Water

1.
College of Traditional Chinese Medicine, Bozhou University, Bozhou 236800, China
2.
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214000, China
3.
Anhui Lei Yun Shang Pharmaceutical Co., Ltd., Bozhou 236800, China

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Received Date: October 12, 2023
Available Online: March 19, 2024

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Abstract

Abstract

Objective: To optimize the subcritical water extraction process for flavonoids from Mentha haplocalyx Briq. (mint) and assess the mitigating effects of peppermint flavonoids on H₂O₂-induced adrenal pheochromocytoma (PC12 cell) injury in rats. Methods: The subcritical water extraction process for mint flavonoids was optimized using single-factor and Box-Behnken response surface tests. A comparison was made with reflux extraction, ultrasonic-assisted extraction, and supercritical CO₂ extraction methods. In vitro cell culture of PC12 cells was employed to establish an H₂O₂-induced PC12 cell injury model. The neuroprotective activity of mint flavonoids was analyzed using the MTT (methyl thiazolye tetrazolium, MTT) method. Results: The optimized extraction conditions were determined to be 142 ℃, 38 min, and 21:1 mL/g, resulting in a mint flavonoid yield of 14.07%±0.23%. This yield was significantly higher (P<0.001) than that of reflux extraction (334% increase), ultrasonic-assisted extraction (75% increase), and supercritical CO₂ extraction (173% increase). Mint flavonoids, within the concentration range of 1~100 μg/mL, significantly enhanced the survival rate of PC12 cells from 53.61%±0.64% to 78.49%±0.84% compared to the H₂O₂ group (P<0.001), indicating substantial alleviation of cellular oxidative injury. Conclusion: The study established an environmentally friendly, efficient, and reliable subcritical water extraction process for mint flavonoids. The extracted mint flavonoids demonstrated significant protective effects against H₂O₂-induced PC12 cell injury (P<0.01).

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