Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from <i>Mentha haplocalyx</i> Briq. with Subcritical Water

ZHANG Qingqing; ZHANG Mengjiao; MA Yiming; CAI Xueyi

doi:10.13386/j.issn1002-0306.2023100088

Volume 45 Issue 10

May 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(10): 17-24. > DOI: 10.13386/j.issn1002-0306.2023100088

ZHANG Qingqing, ZHANG Mengjiao, MA Yiming, et al. Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq. with Subcritical Water[J]. Science and Technology of Food Industry, 2024, 45(10): 17−24. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100088.

Citation:

PDF (4990 KB)

Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq. with Subcritical Water

1.
College of Traditional Chinese Medicine, Bozhou University, Bozhou 236800, China
2.
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214000, China
3.
Anhui Lei Yun Shang Pharmaceutical Co., Ltd., Bozhou 236800, China

More Information

Received Date: October 12, 2023
Available Online: March 19, 2024

Graphical Abstract

Abstract

Abstract

Objective: To optimize the subcritical water extraction process for flavonoids from Mentha haplocalyx Briq. (mint) and assess the mitigating effects of peppermint flavonoids on H₂O₂-induced adrenal pheochromocytoma (PC12 cell) injury in rats. Methods: The subcritical water extraction process for mint flavonoids was optimized using single-factor and Box-Behnken response surface tests. A comparison was made with reflux extraction, ultrasonic-assisted extraction, and supercritical CO₂ extraction methods. In vitro cell culture of PC12 cells was employed to establish an H₂O₂-induced PC12 cell injury model. The neuroprotective activity of mint flavonoids was analyzed using the MTT (methyl thiazolye tetrazolium, MTT) method. Results: The optimized extraction conditions were determined to be 142 ℃, 38 min, and 21:1 mL/g, resulting in a mint flavonoid yield of 14.07%±0.23%. This yield was significantly higher (P<0.001) than that of reflux extraction (334% increase), ultrasonic-assisted extraction (75% increase), and supercritical CO₂ extraction (173% increase). Mint flavonoids, within the concentration range of 1~100 μg/mL, significantly enhanced the survival rate of PC12 cells from 53.61%±0.64% to 78.49%±0.84% compared to the H₂O₂ group (P<0.001), indicating substantial alleviation of cellular oxidative injury. Conclusion: The study established an environmentally friendly, efficient, and reliable subcritical water extraction process for mint flavonoids. The extracted mint flavonoids demonstrated significant protective effects against H₂O₂-induced PC12 cell injury (P<0.01).

FullText(HTML)

References (41)

References

[1]	BLOEM B R, OKUN M S, KLEIN C. Parkinson’s disease[J]. Seminar,2021,397:2284−2303.
[2]	KOVAL L, BÔNE A, LOUIS M, et al. AD Course Map charts Alzheimer’s disease progression[J]. Scientific Reports,2021,11:8020−8035. doi: 10.1038/s41598-021-87434-1
[3]	HAYES M T. Parkinson's disease and parkinsonism[J]. The American Journal of Medicine,2019,132(7):802−807. doi: 10.1016/j.amjmed.2019.03.001
[4]	LUKIW W J. MicroRNA (miRNA) Complexity in Alzheimer’s disease (AD)[J]. Biology,2023,12:788−801. doi: 10.3390/biology12060788
[5]	LUKIW W J, VERGALLO A, LISTA S, et al. Biomarkers for Alzheimer’s disease (AD) and the application of precision medicine[J]. Journal of Personalized Medicine,2020,10:138−149. doi: 10.3390/jpm10030138
[6]	ZHANG D Y, LI X, HE X S, et al. Protective effect of flavonoids against methylglyoxal-induced oxidative stress in PC-12 neuroblastoma cells and its structure–activity relationships[J]. Molecules,2022,27(22):7804−7814. doi: 10.3390/molecules27227804
[7]	MANISH P, LING L W, LIN T S, et al. Flavonoids and its neuroprotective effects on brain ischemia and neurodegenerative diseases[J]. Current Drug Targets,2018,19(14):1710−1720. doi: 10.2174/1389450119666180326125252
[8]	AMINZADEH A, SALARINEJAD A. Effects of myricetin against cadmium-induced neurotoxicity in PC12 cells[J]. Toxicology Research,2021,10:84−90. doi: 10.1093/toxres/tfaa104
[9]	LEE S R, SONG J, SONG J H, et al. Chemical identification of isoflavonoids from a termite-associated streptomyces sp. rb1 and their neuroprotective effects in murine hippocampal HT22 cell line[J]. International Journal of Molecular Sciences,2018,19(9):2640−2652. doi: 10.3390/ijms19092640
[10]	屈赵. 中草药来源黄酮类化合物的神经保护作用及机制研究[D]. 广州:广中中医药大学, 2018. [QU Z. Neuroprotective effects of flavonoids derived from Chinese herbal medicine and their underlying mechanisms exploration[D]. Guangzhou:Guangzhou University of Chinese Medicine, 2018.] QU Z. Neuroprotective effects of flavonoids derived from Chinese herbal medicine and their underlying mechanisms exploration[D]. Guangzhou: Guangzhou University of Chinese Medicine, 2018.
[11]	XING G H, DONG M X, LI X M, et al. Neuroprotective effects of puerarin against beta-amyloid-induced neurotoxicity in PC12 cells via a PI3K-dependent signaling pathway[J]. Brain Res Bull,2011,85(3-4):212−218. doi: 10.1016/j.brainresbull.2011.03.024
[12]	ZHAO Y K, ZHAO J, ZHANG X Y, et al. Botanical drug puerarin promotes neuronal survival and neurite outgrowth against MPTP/MPP⁺-induced toxicity via progesterone receptor signaling[J]. Oxidative Medicine and Cellular Longevity, 2020:7635291.
[13]	LÜ C N, YANG F, QIN R L, et al. Bioactivity-guided isolation of chemical constituents against H₂O₂-induced neurotoxicity on PC12 from Cimicifuga dahurica (Turcz.) Maxim[J]. Bioorganic & Medicinal Chemistry Letters,2017,27:3305−3309.
[14]	陈斌, 刘洁, 詹敏敏, 等. 黄酮类化合物与肠道菌群互作研究进展[J]. 中国食品学报,2022,22(6):369−381. [CHEN B, LIU J, ZAN M M, et al. Research progress in the interaction between flavonoids and gut microbiota[J]. Journal of Chinese Institute of Food Science and Technology,2022,22(6):369−381.] CHEN B, LIU J, ZAN M M, et al. Research progress in the interaction between flavonoids and gut microbiota[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22（6）: 369−381.
[15]	国家药典委员会. 中华人民共和国药典[M]. 北京:中国医药科技出版社, 2020:394−395. [Chinese Pharmacopoeia Commission. Pharmacopoeia of People’s Republic of China[M]. Beijing:China Medical Science Press, 2020:394−395.] Chinese Pharmacopoeia Commission. Pharmacopoeia of People’s Republic of China[M]. Beijing: China Medical Science Press, 2020: 394−395.
[16]	LI Y X, LIU Y B, MA A Q, et al. In vitro antiviral, anti-inflammatory, and antioxidant activities of the ethanol extract of Mentha piperita L.[J]. Food Sci Biotechnol,2017,26(6):1675−1683. doi: 10.1007/s10068-017-0217-9
[17]	杨玉莹, 窦晓鑫, 王方园, 等. 抗新型冠状病毒肺炎“三药三方”之中医理论探讨[J]. 天津中医药,2021,38(6):700−705. [YANG Y Y, DOU X X, WANG F Y, et al. Discussion on traditional Chinese medicine theory of anti-COVID-19 “three drugs and three prescriptions”[J]. Tianjin Journal of Traditional Chinese Medicine,2021,38(6):700−705.] YANG Y Y, DOU X X, WANG F Y, et al. Discussion on traditional Chinese medicine theory of anti-COVID-19 “three drugs and three prescriptions”[J]. Tianjin Journal of Traditional Chinese Medicine, 2021, 38（6）: 700−705.
[18]	HE X F, GENG C A, HUANG X Y, et al. Chemical Constituents from Mentha haplocalyx Briq. (Mentha canadensis L.) and their α-glucosidase inhibitory activities[J]. Natural Products and Bioprospecting,2019,9:223−229. doi: 10.1007/s13659-019-0207-0
[19]	KAPP K, PÜSSA T, ORAV A, et al. Chemical composition and antibacterial effect of Mentha spp. grown in estonia[J]. Natural Product Communications,2020,15(12):1−14.
[20]	LIU R H, WANG Y T, LIANG C L, et al. Morphology and mass spectrometry-based chemical profiling of peltate glandular trichomes on Mentha haplocalyx Briq leaves[J]. Food Research International,2023,164:112323−112333. doi: 10.1016/j.foodres.2022.112323
[21]	XU L L, XU J J, ZHONG K R, et al. Analysis of non-volatile chemical constituents of Menthae haplocalycis Herba by ultra-high performance liquid chromatography-high resolution mass spectrometry[J]. Molecules,2017,22:1756−1772. doi: 10.3390/molecules22101756
[22]	侯学敏, 李林霞, 张直峰, 等. 响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J]. 食品科学,2013,34(6):124−128. [HOU X M, LI L X, ZHANG Z F, et al. Total flavonoids from Mentha haplocalyx Briq. leaves:Optimization of extraction process by response surface methodology and antioxidant activity[J]. Food Science,2013,34(6):124−128.] HOU X M, LI L X, ZHANG Z F, et al. Total flavonoids from Mentha haplocalyx Briq. leaves: Optimization of extraction process by response surface methodology and antioxidant activity[J]. Food Science, 2013, 34（6）: 124−128.
[23]	BELFEKI H. MEJRI M. HASSOUNA M. Antioxidant and anti-lipases activities in vitro of Mentha viridis and Eucalyptus globulus extracts[J]. Industrial Crops and Products,2016(89):514−521.
[24]	刘雪辉, 张思, 张志旭, 等. 大孔吸附树脂纯化薄荷总黄酮工艺优选[J]. 食品与机械,2016,32(8):156−177. [LIU X H, ZHANG S, ZHANG Z X, et al. Optimization of purification technology for total flavonoids from Menthaha plocalyx by macroporous resin[J]. Food & Machinery,2016,32(8):156−177.] LIU X H, ZHANG S, ZHANG Z X, et al. Optimization of purification technology for total flavonoids from Menthaha plocalyx by macroporous resin[J]. Food & Machinery, 2016, 32（8）: 156−177.
[25]	高丹丹, 郭鹏辉, 祁高展, 等. 响应面法优化薄荷全草总黄酮的提取工艺[J]. 食品工业科技, 2015, 36(2):299-322. [GAO D D, GUO P H, QI G Z et al. Optimization of extraction technology of total flavonoids from Mentha haplocalyx by response surface methodology[J]. Science and Technology of Food Industry, 2015, 36(2):299-303.] GAO D D, GUO P H, QI G Z et al. Optimization of extraction technology of total flavonoids from Mentha haplocalyx by response surface methodology[J]. Science and Technology of Food Industry, 2015, 36（2）: 299-303.
[26]	张晴晴, 邢何欢, 龙昌辉, 等. 薄荷中黄酮类化合物萃取工艺优化及成分分析[J]. 延边大学农学学报,2022,44(4):67−72. [ZHANG Q Q, XING H H, LONG C H, et al. Optimization of extraction process of Mentha haplocalyx flavonoids and composition analysis[J]. Agricultural Science Journal of Yanbian University,2022,44(4):67−72.] ZHANG Q Q, XING H H, LONG C H, et al. Optimization of extraction process of Mentha haplocalyx flavonoids and composition analysis[J]. Agricultural Science Journal of Yanbian University, 2022, 44（4）: 67−72.
[27]	CHENG Y, XUE F M, YU S, et al. Subcritical Water Extraction of Natural Products[J]. Molecules,2021,26:4004−4041. doi: 10.3390/molecules26134004
[28]	ZHANG J X, WEN C T, ZHANG H H, et al. Recent advances in the extraction of bioactive compounds with subcritical water:A review[J]. Trends in Food Science & Technology,2020,95:183−195.
[29]	TSAI M, ZHU L, MAEDA S, et al. Extraction of phytochemicals from maypole apple by subcritical water[J]. Foods,2022,11:3453−3464. doi: 10.3390/foods11213453
[30]	KO M J, LEE J H, NAM H H, et al. Subcritical water extraction of phytochemicals from Phlomis umbrosa Turcz[J]. Innovative Food Science and Emerging Technologies,2017,42:1−7. doi: 10.1016/j.ifset.2017.05.009
[31]	CUI Y L, WANG S, WANG S X, et al. Extraction optimization and characterization of persimmon peel pectinextracted by subcritical water[J]. Food Chemistry: X,2022,16:100486−100495. doi: 10.1016/j.fochx.2022.100486
[32]	包怡红, 邓启. 响应面法优化亚临界水萃取黑木耳多糖工艺[J]. 食品与生物技术学报,2016,35(10):1053−1060. [BAO Y H, DENG Q. Optimization of subcritical water extraction of polysaccharides from Auricularia auricular by response surface methodology[J]. Journal of Food Science and Biotechnology,2016,35(10):1053−1060.] BAO Y H, DENG Q. Optimization of subcritical water extraction of polysaccharides from Auricularia auricular by response surface methodology[J]. Journal of Food Science and Biotechnology, 2016, 35（10）: 1053−1060.
[33]	LACHOS-PEREZ D, BASEGGIOB A M, MAYANGA-TORRES P C, et al. Subcritical water extraction of flavanones from defatted orange peel[J]. The Journal of Supercritical Fluids,2018,138:7−16. doi: 10.1016/j.supflu.2018.03.015
[34]	ZHANG H G, LIU S H, LI H Z, et al. Extraction of isoflavones from Puerariae lobata using subcritical water[J]. RSC Advances,2018,8:22652−22658. doi: 10.1039/C8RA02653J
[35]	朱晓晗, 铁芳芳, 欧阳健, 等. 桑葚花色苷对PC12细胞氧化损伤的保护作用[J/OL]. 食品与发酵工业, 1−12 [2024-03-08]. https://doi.org/10.13995/j.cnki.11-1802/ts.035027. [ZHU X H, TIE F F, OU Y J, et al. Protective effects of mulberry anthocyanin on oxidative stress in PC12 cells[J/OL]. Food and Fermentation Industries, 1−12 [2024-03-08]. https://doi.org/10.13995/j.cnki.11-1802/ts.035027.] ZHU X H, TIE F F, OU Y J, et al. Protective effects of mulberry anthocyanin on oxidative stress in PC12 cells[J/OL]. Food and Fermentation Industries, 1−12 [2024-03-08]. https://doi.org/10.13995/j.cnki.11-1802/ts.035027.
[36]	WAN N, KOU P, PANG H Y, et al. Enzyme pretreatment combined with ultrasonic-microwave-assisted surfactant for simultaneous extraction of essential oil and flavonoids from Baeckea frutescens[J]. Industrial Crops and Products,2021,174:114173−114182. doi: 10.1016/j.indcrop.2021.114173
[37]	NKURUNZIZAAD, PENDLETONB P, CHUN B S, et al. Optimization and kinetics modeling of okara isoflavones extraction using subcritical water[J]. Food Chemistry,2019,295:613−621. doi: 10.1016/j.foodchem.2019.05.129
[38]	冀恬, 董琦, 谭亮, 等. 亚临界水萃取康定鼠尾草中丹参酮类成分工艺的优化[J]. 中成药,2017,39(10):2190−2193. [GI T, DONG Q, TAN L, et al. Optimization of extraction process of tanshinones from Salvia Kangding by subcritical water[J]. Chinese Traditional Patent Medicine,2017,39(10):2190−2193.] doi: 10.3969/j.issn.1001-1528.2017.10.045 GI T, DONG Q, TAN L, et al. Optimization of extraction process of tanshinones from Salvia Kangding by subcritical water[J]. Chinese Traditional Patent Medicine, 2017, 39（10）: 2190−2193. doi: 10.3969/j.issn.1001-1528.2017.10.045
[39]	董兴叶, 许明君, 邓辰辰, 等. 亚临界水萃取杜仲叶黄酮类化合物工艺优化研究[J]. 河南工业大学学报(自然科学版),2016,37(1):84−87. [DONG X Y, XU M J, DENG C C, et al. Optimization of subcritical water extraction processing of flavonoids from Eucommia ulmoides leaves[J]. Journal of Henan University of Technology (Natural Science Edition),2016,37(1):84−87.] DONG X Y, XU M J, DENG C C, et al. Optimization of subcritical water extraction processing of flavonoids from Eucommia ulmoides leaves[J]. Journal of Henan University of Technology （Natural Science Edition）, 2016, 37（1）: 84−87.
[40]	LIU J, LI Y C, LIU W Q, et al. Extraction of polysaccharide from Dendrobium nobile Lindl. by subcritical water extraction[J]. ACS Omega,2019,4:20586−20594. doi: 10.1021/acsomega.9b02550
[41]	CHU Q, CHEN M, SONG D X, et al. Apios americana Medik flowers polysaccharide (AFP-2) attenuates H₂O₂ induced neurotoxicity in PC12 cells[J]. International Journal of Biological Macromolecules,2019,123:1115−1124. doi: 10.1016/j.ijbiomac.2018.11.078

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(5)

1.	杨雪丽，牛犇，陈杭君，吴伟杰，王冠楠，房祥军，穆宏磊，郜海燕. 猴头菇纳米囊泡制备、结构表征及抗氧化活性分析. 食品工业科技. 2025(02): 218-230 . 本站查看
2.	刘怡君，王红娟，张靖彬，陈欢，侯宏卫，胡清源. 植物细胞外囊泡作为药物载体的研究进展. 药学学报. 2025(03): 721-730 .
3.	胡群菊，王潮岗，向文洲，王媛媛，范美华，张晓林，廖智，严小军. 微藻细胞外囊泡的研究进展. 水生生物学报. 2024(06): 1051-1064 .
4.	曹桂芳，李丹，伊高阳，杨永利，姚晓琳. 植源性和乳源性胞外囊泡对慢性炎症的调控机制. 中国食品学报. 2024(07): 364-376 .
5.	李俊言，王文苹，张祎，杨枝中. 植物类中药来源囊泡的研究进展. 浙江大学学报(医学版). 2023(03): 349-360 .