Antimicrobial Activity of Extracts from Different Parts of Lonicera japonica Thunb. and Their Antibacterial Properties against Alicyclobacillus acidoterrestris

LIANG Xinhong; HUANG Junchao; TU Cunjian; LI Yongchao; LI Junrui; JIAO Lingxia; RAN Junjian

doi:10.13386/j.issn1002-0306.2023100037

Volume 45 Issue 17

Aug. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(17): 139-146. > DOI: 10.13386/j.issn1002-0306.2023100037

LIANG Xinhong, HUANG Junchao, TU Cunjian, et al. Antimicrobial Activity of Extracts from Different Parts of Lonicera japonica Thunb. and Their Antibacterial Properties against Alicyclobacillus acidoterrestris[J]. Science and Technology of Food Industry, 2024, 45(17): 139−146. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100037.

Citation:

PDF (1993 KB)

Antimicrobial Activity of Extracts from Different Parts of Lonicera japonica Thunb. and Their Antibacterial Properties against Alicyclobacillus acidoterrestris

1.
School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
2.
School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China

More Information

Received Date: October 08, 2023
Available Online: July 04, 2024

Graphical Abstract

Abstract

Abstract

This study investigated the bioactive substance contents within extracts derived from different parts of Lonicera japonica Thunb. and assessed their bacterial inhibitory activities against 12 test strains. Initially, the concentrations of total phenols, total flavonoids, and chlorogenic acid were determined in Lonicera japonica leaves, Lonicerae japonica Flos, and Lonicera japonica caulis. Subsequently, the bacterial inhibition profiles of their extracts against 12 test strains were analyzed. Building upon this analysis, the effects of Lonicerae japonica Flos and Lonicera japonica leaves extracts on minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curve, biofilm formation, and cell morphology of Alicyclobacillus acidoterrestris were further investigated. Results revealed that the concentrations of total phenols, total flavonoids, and chlorogenic acid in L. japonica leaves and L. japonica Flos were significantly higher than those from L. japonica caulis (P<0.05). Moreover, the total flavonoids content in L. japonica leaves was notably higher than that in L. japonica Flos (P<0.05). Three extracts exhibited varying degrees of bacteriostatic effects against A. acidoterrestris, Staphylococcus aureus, Streptococcus agalactiae, Listeria monocytogenes, Bacillus subtilis, Escherichia coli and Salmonella. Notably, extracts of L. japonica leaves and L. japonica Flos displayed superior bacteriostatic effects compared to L. japonica caulis. Extracts of L. japonica leaves and L. japonica Flos exhibited the most significant bacteriostatic effects against A. acidoterrestris, with MIC of 3.91 mg/mL and MBC of 31.25 mg/mL. Furthermore, extracts from L. japonica leaves and L. japonica Flos effectively inhibited the growth and biofilm formation of A. acidoterrestris, causing disruptions to their organism morphology. Notably, the inhibitory effects were augmented with the increase of extract concentrations. This study lays a robust foundation for understanding the mechanisms behind L. japonica inhibition of A. acidoterrestris and offers insights for developing safe and efficient new food bacteriostatic agents.

FullText(HTML)

References (38)

References

[1]	OSOPALE A B, ADEWUMI A G, WITTHUHN C R, et al. A review of innovative techniques for rapid detection and enrichment of Alicyclobacillus during industrial processing of fruit juices and concentrates[J]. Food Control,2019,99:146−157. doi: 10.1016/j.foodcont.2018.12.032
[2]	CAI R, YUAN Y, WANG Z, et al. Effects of preservatives on Alicyclobacillus acidoterrestris growth and guaiacol production[J]. International Journal of Food Microbiology,2015,214:145−150. doi: 10.1016/j.ijfoodmicro.2015.08.013
[3]	PORNPUKDEEWATTANA S, JINDAPRASERT A, MASSA S. Alicyclobacillus spoilage and control-a review[J]. Critical Reviews in Food Science and Nutrition,2020,60(1):108−122. doi: 10.1080/10408398.2018.1516190
[4]	JOVANOVIĆ A A, DJORDJEVIĆ V B, PETROVIĆ P M, et al. The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme[J]. Journal of Applied Research on Medicinal and Aromatic Plants,2021,25:100328. doi: 10.1016/j.jarmap.2021.100328
[5]	DIARRA M S, HASSAN Y I, BLOCK G S, et al. Antibacterial activities of a polyphenolic-rich extract prepared from American cranberry (Vaccinium macrocarpon) fruit pomace against Listeria spp.[J]. LWT-Food Science and Technology,2020,123:109056. doi: 10.1016/j.lwt.2020.109056
[6]	CUI S M, LI T, LIANG H Y, et al. Antibacterial activities and mechanisms of vine tea extract and 2R, 3R-dihydromyricetin on Escherichia coli[J]. LWT-Food Science and Technology,2021,146(1):111393.
[7]	SHANG X, PAN H, LI M, et al. Lonicera japonica Thunb.:Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine[J]. Journal of Ethnopharmacology,2011,138(1):1−21. doi: 10.1016/j.jep.2011.08.016
[8]	LI Y, XIE L, LIU K, et al. Bioactive components and beneficial bioactivities of flowers, stems, leaves of Lonicera japonica Thunberg:A review[J]. Biochemical Systematics and Ecology,2023,106:104570. doi: 10.1016/j.bse.2022.104570
[9]	YE J, SU J, CHEN K, et al. Comparative investigation on chemical constituents of flower bud, stem and leaf of Lonicera japonica Thunb. by HPLC-DAD-ESI-MS/MSⁿ and GC-MS[J]. Journal of Analytical Chemistry,2014,69:777−784. doi: 10.1134/S1061934814080036
[10]	XIONG J, LI S, WANG W, et al. Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves[J]. Food Chemistry,2013,138(1):327−333. doi: 10.1016/j.foodchem.2012.10.127
[11]	赵媛媛, 杨倩茹, 郝江波, 等. 金银花与忍冬藤及叶药理作用差异的研究进展[J]. 中国中药杂志,2016,41(13):2422−2427. [ZHAO Y Y, YANG Q R, HAO J B, et al. Research progress on pharmacological effects and their differences among the flowers, stems and leaves of Lonicera japonica[J]. China Journal of Chinese Materia Medica,2016,41(13):2422−2427.] ZHAO Y Y, YANG Q R, HAO J B, et al. Research progress on pharmacological effects and their differences among the flowers, stems and leaves of Lonicera japonica[J]. China Journal of Chinese Materia Medica, 2016, 41（13）: 2422−2427.
[12]	FAN Z L, LI L, BAI X L, et al. Extraction optimization, antioxidant activity, and tyrosinase inhibitory capacity of polyphenols from Lonicera japonica[J]. Food Science & Nutrition,2019,7(5):1786−1794.
[13]	GE L, LI J, WAN H, et al. Novel flavonoids from Lonicera japonica flower buds and validation of their anti-hepatoma and hepatoprotective activity in vitro studies[J]. Industrial Crops and Products,2018,125:114−122. doi: 10.1016/j.indcrop.2018.08.073
[14]	WANG D, ZHAO X, LIU Y. Hypoglycemic and hypolipidemic effects of a polysaccharide from flower buds of Lonicera japonica in streptozotocin-induced diabetic rats[J]. International Journal of Biological Macromolecules,2017,102:396−404. doi: 10.1016/j.ijbiomac.2017.04.056
[15]	LI Y, CAI W, WENG X, et al. Lonicera japonica Flos and Lonicerae Flos:A systematic pharmacology review[J]. Evidence-Based Complementary and Alternative Medicine,2015,12:905063.
[16]	RAHMAN A, KANG S C. In vitro control of food-borne and food spoilage bacteria by essential oil and ethanol extracts of Lonicera japonica Thunb.[J]. Food Chemistry,2009,116(3):670−675. doi: 10.1016/j.foodchem.2009.03.014
[17]	马艳妮, 王志尧, 郭展展, 等. 忍冬叶总黄酮的测定及其体外抗菌活性[J]. 中国实验方剂学杂志,2017,23(6):55−59. [MA Y N, WANG Z Y, GUO Z Z, et al. Determination of total flavonoids of Lonicera japonica leaves and their in vitro antibacterial activity[J]. Chinese Journal of Experimental Traditional Medical Formulae,2017,23(6):55−59.] MA Y N, WANG Z Y, GUO Z Z, et al. Determination of total flavonoids of Lonicera japonica leaves and their in vitro antibacterial activity[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23（6）: 55−59.
[18]	李磊, 谭政委, 余永亮, 等. 金银花种质资源及品种选育研究进展[J]. 安徽农业科学,2022,50(17):1−4. [LI L, TAN Z W, YU Y L, et al. Research progress on germplasm resources and breeding of Lonicera japonica Thunb doi: 10.3969/j.issn.0517-6611.2022.17.001 J]. Journal of Anhui Agricultural Sciences,2022,50(17):1−4. doi: 10.3969/j.issn.0517-6611.2022.17.001
[19]	HSU H F, HSIAO P C, KUO T C, et al. Antioxidant and anti-inflammatory activities of Lonicera japonica Thunb. var. sempervillosa Hayata flower bud extracts prepared by water, ethanol and supercritical fluid extraction techniques[J]. Industrial Crops & Products,2016,89:543−549.
[20]	KWAW E, MA Y, TCHABO W, et al. Effect of Lactobacillus strains on phenolic profile, color attributes and antioxidant activities of lactic-acid fermented mulberry juice[J]. Food Chemistry,2018,250:148−154. doi: 10.1016/j.foodchem.2018.01.009
[21]	OTEEF M D Y. Comparison of different extraction techniques and conditions for optimizing an HPLC-DAD method for the routine determination of the content of chlorogenic acids in green coffee beans[J]. Separations,2022,9(12):396. doi: 10.3390/separations9120396
[22]	YANG Y J, LIN M Y, FENG S Y, et al. Chemical composition, antibacterial activity, and mechanism of action of essential oil from Litsea cubeba against foodborne bacteria[J]. Journal of Food Processing and Preservation,2020,44(9):e14724.
[23]	BĄCZEK K B, KOSAKOWSKA O, PRZYBYŁ, J L, et al. Antibacterial and antioxidant activity of essential oils and extracts from costmary (Tanacetum balsamita L.) and tansy (Tanacetum vulgare L.)[J]. Industrial Crops & Products,2017,102:154−163.
[24]	LIN L, AGYEMANG K, ABDEL-SAMIE M A S, et al. Antibacterial mechanism of Tetrapleura tetraptera extract against Escherichia coli and Staphylococcus aureus and its application in pork[J]. Journal of Food Safety,2019,39(6):e12693. doi: 10.1111/jfs.12693
[25]	CUI H Y, ZHOU H, LIN L. The specific antibacterial effect of the Salvia oil nanoliposomes against Staphylococcus aureus biofilms on milk container[J]. Food Control,2016,61:92−98. doi: 10.1016/j.foodcont.2015.09.034
[26]	CHEN M, ZHAO Z, MENG H, et al. The antibiotic activity and mechanisms of sugar beet (Beta vulgaris) molasses polyphenols against selected food-borne pathogens[J]. LWT-Food Science and Technology,2017,82:354−360. doi: 10.1016/j.lwt.2017.04.063
[27]	GHADERI L, ALIAHMADI A, EBRAHIMI S N, et al. Effective inhibition and eradication of Pseudomonas aeruginosa biofilms by Satureja khuzistanica essential oil nanoemulsion[J]. Journal of Drug Delivery Science and Technology,2020,61:102260.
[28]	章艳玲. 金银花不同部位化学成分的比较研究[J]. 农学学报,2019,9(1):59−61. [ZHANG Y L. A comparison of chemical constituents in different parts of honeysuckle[J]. Journal of Agriculture,2019,9(1):59−61.] doi: 10.11923/j.issn.2095-4050.cjas18030005 ZHANG Y L. A comparison of chemical constituents in different parts of honeysuckle[J]. Journal of Agriculture, 2019, 9（1）: 59−61. doi: 10.11923/j.issn.2095-4050.cjas18030005
[29]	路俊仙, 吕凌, 张才波. 忍冬4个药用部位中11种化学成分的含量测定[J]. 中药材,2019,42(5):1085−1089. [LU J X, LÜ L, ZHANG C B. Determination of 11 chemical constituents in 4 medicinal parts of Lonicera japonica Thunb J]. Journal of Chinese Medicinal Materials,2019,42(5):1085−1089.
[30]	贾海燕, 滕旭东, 王慧, 等. 复方忍冬藤提取物促进骨折愈合及抗炎作用研究[J]. 中国畜牧兽医,2017,44(1):275−281. [JIA H Y, TENG X D, WANG H, et al. Effects of mixed caulis Lonicerae japonicae extracts on fracture healing and anti-inflammation[J]. China Animal Husbandry & Veterinary Medicine,2017,44(1):275−281.] JIA H Y, TENG X D, WANG H, et al. Effects of mixed caulis Lonicerae japonicae extracts on fracture healing and anti-inflammation[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44（1）: 275−281.
[31]	范帅帅. 忍冬不同部位含量测定和指纹图谱结合化学模式识别的研究[D]. 石家庄:河北中医学院, 2019. [FAN S S. Study on content determination of different parts of Lonicera japonica and fingerprint combined with chemical pattern recognition[D]. Shijiazhuang:Hebei University of Chinese Medicine, 2019.] FAN S S. Study on content determination of different parts of Lonicera japonica and fingerprint combined with chemical pattern recognition[D]. Shijiazhuang: Hebei University of Chinese Medicine, 2019.
[32]	YAN L, XIE Y, WANG Y, et al. Variation in contents of active components and antibacterial activity in different parts of Lonicera japonica Thunb.[J]. Asian Biomedicine,2020,14(1):19−26. doi: 10.1515/abm-2020-0004
[33]	任敏, 金振锐, 徐小博, 等. 金银花不同器官提取物抑菌作用研究[J]. 安徽农学通报,2019,29(9):41−42. [REN M, JIN Z R, XU X B, et al. Study on bacteriostasis of extracts from different organs of Lonicera japonica Thunb doi: 10.3969/j.issn.1007-7731.2019.09.014 J]. Anhui Agricultural Science Bulletin,2019,29(9):41−42. doi: 10.3969/j.issn.1007-7731.2019.09.014
[34]	NIE R Z, DANG M Z, GE Z Z, et al. Interactions of chlorogenic acid and isochlorogenic acid A with model lipid bilayer membranes:Insights from molecular dynamics simulations[J]. Chemistry and Physics of Lipids,2021,240:105136. doi: 10.1016/j.chemphyslip.2021.105136
[35]	CAI R, MIAO M, YUE T, et al. Antibacterial activity and mechanism of cinnamic acid and chlorogenic acid against Alicyclobacillus acidoterrestris vegetative cells in apple juice[J]. International Journal of Food Science & Technology,2019,54(5):1697−1705.
[36]	LI J, ZHAO N, XU R, et al. Deciphering the antibacterial activity and mechanism of p-coumaric acid against Alicyclobacillus acidoterrestris and its application in apple juice[J]. International Journal of Food Microbiology,2022,378:109822. doi: 10.1016/j.ijfoodmicro.2022.109822
[37]	STEPANOVIĆ S, VUKOVIĆ D, HOLA V, et al. Quantification of biofilm in microtiter plates:Overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci[J]. Apmis,2007,115(8):891−899. doi: 10.1111/j.1600-0463.2007.apm_630.x
[38]	JUNG J H, CHOI N Y, LEE S Y. Biofilm formation and exopolysaccharide (EPS) production by Cronobacter sakazakii depending on environmental conditions[J]. Food Microbiology,2013,34(1):70−80. doi: 10.1016/j.fm.2012.11.008