Combination of Bacillomycin D and Chitosan for Microbial Inhibition and Freshness Preservation of Sugar Orange

SUN Jing; LIU Yujuan; LUO Xiaojiao; ZHANG Moran; HUANG Junkai; DAI Yongjin; LU Yingjian

doi:10.13386/j.issn1002-0306.2023100014

Volume 45 Issue 17

Aug. 2024

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Science and Technology of Food Industry > 2024 > 45(17): 372-379. > DOI: 10.13386/j.issn1002-0306.2023100014

SUN Jing, LIU Yujuan, LUO Xiaojiao, et al. Combination of Bacillomycin D and Chitosan for Microbial Inhibition and Freshness Preservation of Sugar Orange[J]. Science and Technology of Food Industry, 2024, 45(17): 372−379. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100014.

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Combination of Bacillomycin D and Chitosan for Microbial Inhibition and Freshness Preservation of Sugar Orange

College of Food Science and Engineering, Nanjing University of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing 210023, China

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Received Date: October 08, 2023
Available Online: June 30, 2024

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Abstract

Abstract

Objective: Based on the inhibitory effect of bacillomycin D (BD) and chitosan (CTS) on Penicillium digitatum, the effect of BD and CTS combination on post-harvest disease control and freshness preservation of sugar oranges was investigated. Method: Sugar oranges as the research object, mold strains were screened in the moldy sugar oranges for identification, and the minimum inhibitory concentration and FIC index of BD and CTS against P. digitatum were determined by the checkerboard microdilution method. Finally, treated sugar oranges with BD and CTS were stored and preserved under the conditions of 28 ℃ and 90% relative humidity, and the changes of morbidity, weight loss, hardness, soluble solids, enzyme activity and other indicators were determined. Result: The isolated and purified fungus from the sugar orange was identified as P. digitatum and named P. digitatum strain L6. The minimum inhibitory concentrations of BD and CTS for P. digitatum were 42.19 and 62.5 mg/L, respectively, and the FIC index was 0.75, indicating that the inhibition of BD and CTS on P. digitatum was cumulative. The storage and preservation results showed that after 15 days of storage, the weight loss rates of BD, CTS, and BD+CTS treated sugar oranges were 0.99%, 1.62%, and 1.09%, respectively, which were 66.44%, 45.08%, and 63.05% lower compared to the control groups. In addition, the results of changes in titratable acidity, soluble solids, ascorbic acid, and plant-related enzyme activities also indicated that BD, CTS, and BD+CTS combination treatments had good preservation effects on sugar oranges, with the BD+CTS combination treatment group having the best results. Conclusion: Both BD and CTS had significant inhibition effect on P. digitatum, which could be used for storage and preservation of sugar orange to extend the shelf life. The combination of the two could not only enhance the preservation effect, but also the low price of CTS could reduce the use of BD and save the cost.
- bacillomycin D,
- chitosan,
- sugar orange,
- antifungal,
- retain freshness

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References

[1]	REYMICK O O, LIU D, CHENG Y, et al. Cuminaldehyde-induced oxidative stress inhibits growth of Penicillium digitatum in citrus[J]. Postharvest Biology and Technology,2022,192:111991. doi: 10.1016/j.postharvbio.2022.111991
[2]	YANG Q, QIAN X, ROUTLEDGE M N, et al. Metabonomics analysis of postharvest citrus response to Penicillium digitatum infection[J]. LWT - Food Science and Technology,2021,152:112371. doi: 10.1016/j.lwt.2021.112371
[3]	LIN S H, LUO P, YUAN E, et al. Physiological and proteomic analysis of Penicillium digitatum in response to X33 antifungal extract treatment[J]. Frontiers in Microbiology,2020,11:584331. doi: 10.3389/fmicb.2020.584331
[4]	PAPOUTSIS K, MATHIOUDAKIS M M, HASPERUÉ J H, et al. Non-chemical treatments for preventing the postharvest fungal rotting of citrus caused by Penicillium digitatum (green mold) and Penicillium italicum (blue mold)[J]. Trends in Food Science & Technology,2019,86:479−491.
[5]	马芳芬, 殷海成. 枯草芽孢杆菌及其杆菌霉素D对黄曲霉毒素作用机制的研究进展[J]. 粮食与饲料工业, 2016, 12(4):48−50. [MA F F, YING H C. Research progress on the inhibition mechanism of Bacillus subtilis and bacillomycin D against aflatoxin[J] Cereal & Feed Industry, 2016, 12(4):48−50.] MA F F, YING H C. Research progress on the inhibition mechanism of Bacillus subtilis and bacillomycin D against aflatoxin[J] Cereal & Feed Industry, 2016, 12（4）: 48−50.
[6]	LIN F, XUE Y, HUANG Z, et al. Bacillomycin D inhibits growth of Rhizopus stolonifer and induces defense-related mechanism in cherry tomato[J]. Applied Microbiology Biotechnology,2019,103(18):7663−7674. doi: 10.1007/s00253-019-09991-w
[7]	王安杏, 曹川, 张庆, 等. 壳聚糖复合膜在果蔬保鲜中的应用[J]. 食品安全质量检测学报, 2023, 14(5):164−172. [WANG A X, CAO C, ZHANG Q, et al. Application of chitosan composite film in fruits and vegetables preservation[J] Journal of Food Safety & Quality, 2023, 14(5):164−172.] WANG A X, CAO C, ZHANG Q, et al. Application of chitosan composite film in fruits and vegetables preservation[J] Journal of Food Safety & Quality, 2023, 14（5）: 164−172.
[8]	胡诗瑶, 王艳颖, 李嘉骏, 等. 壳聚糖复合涂膜处理对鲜切库尔勒梨生理生化品质的影响[J]. 现代园艺,2023,46(3):6−8,11. [HU S Y, WANG Y Y, LI J J, et al. Effects of chitosan composite coating on physiological and biochemical qualities of fresh cut Korla Pear[J]. Contemporary Horticulture,2023,46(3):6−8,11.] HU S Y, WANG Y Y, LI J J, et al. Effects of chitosan composite coating on physiological and biochemical qualities of fresh cut Korla Pear[J]. Contemporary Horticulture, 2023, 46（3）: 6−8,11.
[9]	MEDRANO-CASTELLÓN L M, BLANCAS-BENITEZ F J, SANTOYO-GONZÁLEZ M A, et al. Application of bioactive chitosan-based coatings with marine antagonists to control postharvest Persian lime decay caused by Penicillium italicum[J]. Biocontrol Science Technology,2023,33(3):283−295. doi: 10.1080/09583157.2023.2177258
[10]	COUTINHO T C, FERREIRA M C, ROSA L H, et al. Penicillium citrinum and Penicillium mallochii:New phytopathogens of orange fruit and their control using chitosan[J]. Carbohydrate Polymers,2020,234:115918. doi: 10.1016/j.carbpol.2020.115918
[11]	林福兴. 抗菌脂肽Bacillomycin D对樱桃番茄果实采后病害的防治及其机理研究[D]. 南京:南京农业大学, 2020. [LIN F X. Biocontrol of cherry tomato postharvest diseases by antimicrobial lipopeptide bacillomycin D and its mechanism [D]. Nanjing:Nanjing Agricultural University, 2020.] LIN F X. Biocontrol of cherry tomato postharvest diseases by antimicrobial lipopeptide bacillomycin D and its mechanism [D]. Nanjing: Nanjing Agricultural University, 2020.
[12]	AKBUDAK B, AKBUDAK N, SENIZ V, et al. Sequential treatments of hot water and modified atmosphere packaging in cherry tomatoes[J]. Journal of Food Quality,2007,30(6):896−910. doi: 10.1111/j.1745-4557.2007.00168.x
[13]	WAEWTHONGRAK W, PISUCHPEN S, LEELASUPHAKUL W. Effect of Bacillus subtilis and chitosan applications on green mold (Penicilium digitatum Sacc.) decay in citrus fruit[J]. Postharvest Biology & Technology,2015,99:44−49.
[14]	WAEWTHONGRAK W, LEELASUPHAKUL W, MCCOLLUM G. Cyclic lipopeptides from Bacillus subtilis ABS-S14 elicit defense-related gene expression in citrus fruit[J]. PLoS One,2014,9(10):e109386. doi: 10.1371/journal.pone.0109386
[15]	GONG Q, ZHANG C, LU F, et al. Identification of bacillomycin D from Bacillus subtilis fmbJ and its inhibition effects against Aspergillus flavus[J]. Food Control,2014,36(1):8−14. doi: 10.1016/j.foodcont.2013.07.034
[16]	YAN C. Antimicrobial properties of chitosan and chitosan derivatives in the treatment of enteric infections[J]. Molecules,2021,26(23):7136. doi: 10.3390/molecules26237136
[17]	LORON A, WANG Y, ATANASOVA V, et al. Chitosan for eco-friendly control of mycotoxinogenic Fusarium graminearum[J]. Food Hydrocolloids,2023,134:108067. doi: 10.1016/j.foodhyd.2022.108067
[18]	ARDILA N, DAIGLE F, HEUZEY M C, et al. Effect of chitosan physical form on its antibacterial activity against pathogenic bacteria[J]. Journal of Food Science,2017,82(3):679−686. doi: 10.1111/1750-3841.13635
[19]	JOVANOVI G D, KLAUS A S, NIKI M P. Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish[J]. Revista Argentina De Microbiologia,2016,48(2):128−136. doi: 10.1016/j.ram.2016.02.003
[20]	YAO S, CAO Q, XIE J, et al. Alteration of sugar and organic acid metabolism in postharvest granulation of Ponkan fruit revealed by transcriptome profiling[J]. Postharvest Biology and Technology,2018,139:2−11. doi: 10.1016/j.postharvbio.2018.01.003
[21]	TESFAY S Z, MAGWAZA L S. Evaluating the efficacy of moringa leaf extract, chitosan and carboxymethyl cellulose as edible coatings for enhancing quality and extending postharvest life of avocado (Persea americana Mill.) fruit[J]. Food Packaging & Shelf Life,2017,11:40−48.
[22]	DAN X, QIN H, DAN R. Prolonged preservation of tangerine fruits using chitosan/montmorillonite composite coating[J]. Postharvest Biology & Technology,2018,143:50−57.
[23]	CHIEN P J, SHEU F, LIN H R. Coating citrus (Murcott tangor) fruit with low molecular weight chitosan increases postharvest quality and shelf life[J]. Food Chemistry,2007,100(3):1160−1164. doi: 10.1016/j.foodchem.2005.10.068
[24]	NAYAK S L, SETHI S, SINGH B, et al. Influence of γ-radiation and chitosan coating on postharvest quality of ‘Dancy’ Tangerine fruit[J]. Journal of Packaging Technology and Research,2022,7(1):23−33.
[25]	KAYA, ČESONIENE, DAUBARAS, et al. Chitosan coating of red kiwifruit (Actinidia melanandra) for extending of the shelf life[J]. International Journal of Biological Macromolecules,2016,85:355−360. doi: 10.1016/j.ijbiomac.2016.01.012
[26]	BOWLER C, VAN CAMP W, VAN MONTAGU M, et al. Superoxide dismutase in plants[J]. Critical Reviews in Plant Sciences,1994,13(3):199−218. doi: 10.1080/07352689409701914
[27]	吴彩娥, 李婷婷, 范龚健, 等. 保鲜剂处理对银杏果采后生理及贮藏品质的影响[J]. 中国农业科学,2012,45(18):3832−3840. [WU C E, LI T T, FAN G J, et al. Effect of different preservatives on post-harvest physiological and storage quality in GInkgo biloba seeds[J]. Scientia Agricultura Sinica,2012,45(18):3832−3840.] doi: 10.3864/j.issn.0578-1752.2012.18.016 WU C E, LI T T, FAN G J, et al. Effect of different preservatives on post-harvest physiological and storage quality in GInkgo biloba seeds[J]. Scientia Agricultura Sinica, 2012, 45（18）: 3832−3840. doi: 10.3864/j.issn.0578-1752.2012.18.016
[28]	程晨. 柑橘采后杀菌剂的筛选及防腐保鲜研究[D]. 武汉:华中农业大学, 2017. [CHENG C. Screening of citrus postharvest fungicide and study on antiseptic preservation [D]. Wuhan:Huazhong Agricultural University, 2017.] CHENG C. Screening of citrus postharvest fungicide and study on antiseptic preservation [D]. Wuhan: Huazhong Agricultural University, 2017.
[29]	牛锐. 臭氧处理对柑橘保鲜效果的研究[D]. 晋中:山西农业大学, 2015. [NIU R. Research of ozone treatment effect on citrus preservation [D]. Jinzhong:Shanxi Agricultural University, 2015.] NIU R. Research of ozone treatment effect on citrus preservation [D]. Jinzhong: Shanxi Agricultural University, 2015.
[30]	LIU J, TIAN S P, MENG X H. Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit[J]. Postharvest Biology and Technology,2007,44(3):300−306. doi: 10.1016/j.postharvbio.2006.12.019

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