WU Cunhui, QIN Zhiyang, ZHANG Haizhi. Colorimetric Biosensor Based on Fe-HHTP@ZIF-8 for the Detection of Total Antioxidant Capacity in Beverages[J]. Science and Technology of Food Industry, 2024, 45(17): 327−335. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090323.
Citation: WU Cunhui, QIN Zhiyang, ZHANG Haizhi. Colorimetric Biosensor Based on Fe-HHTP@ZIF-8 for the Detection of Total Antioxidant Capacity in Beverages[J]. Science and Technology of Food Industry, 2024, 45(17): 327−335. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090323.

Colorimetric Biosensor Based on Fe-HHTP@ZIF-8 for the Detection of Total Antioxidant Capacity in Beverages

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  • Received Date: October 08, 2023
  • Available Online: June 30, 2024
  • Total antioxidant capacity of food is one of the critical parameters to evaluate its efficacy. Establishment of a fast and sensitive analytical method for the detection of total antioxidant capacity of food has great significance. In this paper, a metal-organic framework complex nanozyme (Fe-HHTP@ZIF-8) with peroxidase-like activity was firstly designed and successfully synthesized. Then, the morphology, structure and catalytic activity were characterized in detail. The results showed that Fe-HHTP@ZIF-8 possessed a two-dimensional ultra-thin layer structure while its Km and Vmax values towards H2O2 were 0.67 mmol/L and 3.55×10−8 mol/L·s−1, respectively. Ascorbic acid (AA) as a typical antioxidant, an efficient colorimetric biosensor was constructed based on its inhibitory effect on chromogenic reaction catalyzed by nanozyme. Comprehensive validation showed that the colorimetric biosensor had low limit of detection (1.67 μmol/L), wide linear range (5.0~3000.0 μmol/L) and high accuracy (recovery rate of 98.62%~103.13%). By using this colorimetric method, total antioxidant capacity of different beverages was detected and the obtained results were basically consistent with labeled values. Therefore, this study provided a simple, fast and effective colorimetric method for the evaluation of the total antioxidant capacity of food.
  • [1]
    HONG C, CHEN L, HUANG J, et al. Gold nanoparticle-decorated MoSe2 nanosheets as highly effective peroxidase-like nanozymes for total antioxidant capacity assay[J]. Nano Research,2023,16(5):7180−7186. doi: 10.1007/s12274-022-5328-9
    [2]
    WANG X, WEI G, LIU W, et al. Platinum-nickel nanoparticles with enhanced oxidase-like activity for total antioxidant capacity bioassay[J]. Analytical Chemistry,2023,95(14):5937−5945. doi: 10.1021/acs.analchem.2c05425
    [3]
    PENG L J, ZHANG C Y, ZHANG W Y, et al. The peroxidase-like catalytic activity of in situ prepared cobalt carbonate and its applications in colorimetric detection of hydrogen peroxide, glucose and ascorbic acid[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2022,651:129744.
    [4]
    朱碧宁. 高效液相色谱电化学发光法检测抗坏血酸的效果研究[J]. 山西化工,2019,39(3):50−51,58. [ZHU B N. Study on the determination of ascorbic acid by high performance liquid chromatography electrochemiluminescence[J]. Shanxi Chemical Industry,2019,39(3):50−51,58.]

    ZHU B N. Study on the determination of ascorbic acid by high performance liquid chromatography electrochemiluminescence[J]. Shanxi Chemical Industry, 2019, 39(3): 50−51,58.
    [5]
    沈海波, 张连钢, 周鑫魁, 等. 基于高灵敏度荧光衍生剂的痕量维生素C液相检测方法建立[J]. 食品工业科技,2020,41(23):272−276,283. [SHEN H B, ZHANG L G, ZHOU X K, et al. A HPLC method for the determination of trace vitamin C based on high sensitivity fluorescent derivatives was established[J]. Science and Technology of Food Industry,2020,41(23):272−276,283.]

    SHEN H B, ZHANG L G, ZHOU X K, et al. A HPLC method for the determination of trace vitamin C based on high sensitivity fluorescent derivatives was established[J]. Science and Technology of Food Industry, 2020, 41(23): 272−276,283.
    [6]
    贾宝珠, 蔡美玲, 邱芷靖, 等. 基于CoOOH纳米片氧化酶活性的比率荧光传感器检测抗坏血酸[J]. 食品工业科技,2022,43(8):273−280. [JIA B Z, CAI M L, QIU Z J, et al. A ratio fluorescence sensor based on oxidase activity of CoOOH nanosheets for the detection of ascorbic acid[J]. Science and Technology of Food Industry,2022,43(8):273−280.]

    JIA B Z, CAI M L, QIU Z J, et al. A ratio fluorescence sensor based on oxidase activity of CoOOH nanosheets for the detection of ascorbic acid[J]. Science and Technology of Food Industry, 2022, 43(8): 273−280.
    [7]
    PENG J, LING J, ZHANG X Q, et al. A rapid, sensitive and selective colorimetric method for detection of ascorbic acid[J]. Sensors and Actuators B:Chemical,2015,221:708−716. doi: 10.1016/j.snb.2015.07.002
    [8]
    WEI H, WANG E. Nanomaterials with enzyme-like characteristics (nanozymes):Next-generation artificial enzymes[J]. Chemical Society Reviews,2013,42(14):6060−6093. doi: 10.1039/c3cs35486e
    [9]
    LIU B, LIU J. Surface modification of nanozymes[J]. Nano Research,2017,10:1125−1148. doi: 10.1007/s12274-017-1426-5
    [10]
    CHEN Y, JIAO L, YAN H, et al. Hierarchically porous S/N codoped carbon nanozymes with enhanced peroxidase-like activity for total antioxidant capacity biosensing[J]. Analytical Chemistry,2020,92(19):13518−13524. doi: 10.1021/acs.analchem.0c02982
    [11]
    LI S, KEOINGTHONG P, XU J, et al. Highly efficient carbon supported Co-Ir nanozyme for the determination of total antioxidant capacity in foods[J]. Biosensors and Bioelectronics,2023,236:115416. doi: 10.1016/j.bios.2023.115416
    [12]
    HAN X, LIU L, GONG H, et al. Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food[J]. Food Chemistry,2022,371:131115. doi: 10.1016/j.foodchem.2021.131115
    [13]
    LIANG N, GE X, ZHAO Y, et al. Promoting sensitive colorimetric detection of hydroquinone and Hg2+ via ZIF-8 dispersion enhanced oxidase-mimicking activity of MnO2 nanozyme[J]. Journal of Hazardous Materials,2023,454:131455. doi: 10.1016/j.jhazmat.2023.131455
    [14]
    LIU J, YUAN Y, CHENG Y, et al. Copper-based metal-organic framework overcomes cancer chemoresistance through systemically disrupting dynamically balanced cellular redox homeostasis[J]. Journal of the American Chemical Society,2022,144(11):4799−4809. doi: 10.1021/jacs.1c11856
    [15]
    KEUM C, PARK S, LEE S Y. Cancer-cell imaging using copper-doped zeolite imidazole framework-8 nanocrystals exhibiting oxidative catalytic activity[J]. Chemistry-An Asian Journal,2018,13(18):2641−2648. doi: 10.1002/asia.201800749
    [16]
    GUO D, LI C, LIU G, et al. Oxidase mimetic activity of a metalloporphyrin-containing porous organic polymer and its applications for colorimetric detection of both ascorbic acid and glutathione[J]. ACS Sustainable Chemistry & Engineering,2021,9(15):5412−5421.
    [17]
    周晨雨, 房琦, 张玉, 等. 基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸[J]. 分析化学,2021,49(6):982−991. [ZHOU C Y, FANG Q, ZHANG Y, et al. Visual detection of ascorbic acid by simulated enzyme based on Au@Pt nanoparticle parental aerogel[J]. Analytical Chemistry,2021,49(6):982−991.]

    ZHOU C Y, FANG Q, ZHANG Y, et al. Visual detection of ascorbic acid by simulated enzyme based on Au@Pt nanoparticle parental aerogel[J]. Analytical Chemistry, 2021, 49(6): 982−991.
    [18]
    ZHU H, LIU B, WANG M, et al. Amorphous Fe-containing phosphotungstates featuring efficient peroxidase-like activity at neutral pH:Toward portable swabs for pesticide detection with tandem catalytic amplification[J]. Analytical Chemistry,2023,95(10):4776−4785. doi: 10.1021/acs.analchem.3c00008
    [19]
    ZHANG D, LIU J, DU P, et al. Cross-linked surface engineering to improve iron porphyrin catalytic activity[J]. Small,2020,16(17):1905889. doi: 10.1002/smll.201905889
    [20]
    ZHANG H Z, WU H Y, QIN X G, et al. Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder[J]. Food Chemistry,2022,384:132552. doi: 10.1016/j.foodchem.2022.132552
    [21]
    YANG H Y, SUN Z P, QIN X G, et al. Ultrasmall Au nanoparticles modified 2D metalloporphyrinic metal-organic framework nanosheets with high peroxidase-like activity for colorimetric detection of organophosphorus pesticides[J]. Food Chemistry,2022,376:131906. doi: 10.1016/j.foodchem.2021.131906
    [22]
    WU H Y, XU Z L, XIONG D N, et al. Two dimensional iron metal-organic framework nanosheet with peroxidase-mimicking activity for colorimetric detection of hypoxanthine related to shrimp freshness[J]. Talanta,2023:124833.
    [23]
    RAINERI M, WINKLER E L, TORRES T E, et al. Effects of biological buffer solutions on the peroxidase-like catalytic activity of Fe3O4 nanoparticles[J]. Nanoscale,2019,11(39):18393−18406. doi: 10.1039/C9NR05799D
    [24]
    YANG W, YANG X, ZHU L, et al. Nanozymes:Activity origin, catalytic mechanism, and biological application[J]. Coordination Chemistry Reviews,2021,448:214170. doi: 10.1016/j.ccr.2021.214170
    [25]
    GUO L, LIANG M, WANG X, et al. The role of L-histidine as molecular tongs:A strategy of grasping Tb3+ using ZIF-8 to design sensors for monitoring an anthrax biomarker on-the-spot[J]. Chemical Science,2020,11(9):2407−2413. doi: 10.1039/D0SC00030B
    [26]
    ZHANG Y, WANG F, LIU C, et al. Nanozyme decorated metal-organic frameworks for enhanced photodynamic therapy[J]. ACS Nano,2018,12(1):651−661. doi: 10.1021/acsnano.7b07746
    [27]
    KONG J, ZHENG J, LI Z, et al. One-pot synthesis of AuAgPd trimetallic nanoparticles with peroxidase-like activity for colorimetric assays[J]. Analytical and Bioanalytical Chemistry,2021,413:5383−5393. doi: 10.1007/s00216-021-03514-1
    [28]
    GAO L, ZHUANG J, NIE L, et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J]. Nature nanotechnology,2007,2(9):577−583. doi: 10.1038/nnano.2007.260
    [29]
    WANG S, XU D, MA L, et al. Ultrathin ZIF-67 nanosheets as a colorimetric biosensing platform for peroxidase-like catalysis[J]. Analytical and Bioanalytical Chemistry,2018,410:7145−7152. doi: 10.1007/s00216-018-1317-y
    [30]
    YU H, WU H, TIAN X, et al. A nano-sized Cu-MOF with high peroxidase-like activity and its potential application in colorimetric detection of H2O2 and glucose[J]. RSC Advances,2021,11(43):26963−26973. doi: 10.1039/D1RA04877E
    [31]
    LÜ J, ZHANG C, WANG S, et al. MOF-derived porous ZnO-Co3O4 nanocages as peroxidase mimics for colorimetric detection of copper(ii) ions in serum[J]. Analyst,2021,146(2):605−611. doi: 10.1039/D0AN01383H
    [32]
    GE K, SUN S, ZHAO Y, et al. Facile synthesis of two-dimensional iron/cobalt metal-organic framework for efficient oxygen evolution electrocatalysis[J]. Angewandte Chemie International Edition,2021,60(21):12097−12102. doi: 10.1002/anie.202102632
    [33]
    WANG Y, ZHAO M, PING J, et al. Bioinspired design of ultrathin 2D bimetallic metal-organic-framework nanosheets used as biomimetic enzymes[J]. Advanced Materials,2016,28(21):4149−4155. doi: 10.1002/adma.201600108
    [34]
    UZUNOGLU D, ÖZER A. Facile synthesis of magnetic iron-based nanoparticles from the leach solution of hyperaccumulator plant pinus brutia for the antibacterial activity and colorimetric detection of ascorbic acid[J]. ACS Applied Bio Materials,2022,5(11):5465−5476. doi: 10.1021/acsabm.2c00782
    [35]
    王若男, 孟佩俊, 李淑荣, 等. 纳米二氧化铈/石墨烯传感器的构建及其对饮品中抗坏血酸的检测[J]. 现代化工,2023,43(8):236−240,245. [WANG R N, MENG P J, LI S R, et al. Construction of nano-cerium dioxide/graphene sensor and its detection of ascorbic acid in drinks[J]. Modern Chemical Industry,2023,43(8):236−240,245.]

    WANG R N, MENG P J, LI S R, et al. Construction of nano-cerium dioxide/graphene sensor and its detection of ascorbic acid in drinks[J]. Modern Chemical Industry, 2023, 43(8): 236−240,245.
    [36]
    KEELEY G P, O'NEILL A, MCEVOY N, et al. Electrochemical ascorbic acid sensor based on DMF-exfoliated graphene[J]. Journal of Materials Chemistry,2010,20(36):7864−7869. doi: 10.1039/c0jm01527j
    [37]
    YANG L, LIU D, HUANG J, et al. Simultaneous determination of dopamine, ascorbic acid and uric acid at electrochemically reduced graphene oxide modified electrode[J]. Sensors and Actuators B:Chemical,2014,193:166−172. doi: 10.1016/j.snb.2013.11.104
    [38]
    WANG C, DU J, WANG H, et al. A facile electrochemical sensor based on reduced graphene oxide and Au nanoplates modified glassy carbon electrode for simultaneous detection of ascorbic acid, dopamine and uric acid[J]. Sensors and Actuators B:Chemical,2014,204:302−309. doi: 10.1016/j.snb.2014.07.077
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