Effects of <i>Lactobacillus acidophilus</i> LA15 on Immune Enhancement and Regulation of Gut Microbiota in Mice

CHEN Zhixian; SHI Lei; ZHANG Yan

doi:10.13386/j.issn1002-0306.2023090192

Volume 46 Issue 5

Feb. 2025

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Science and Technology of Food Industry > 2025 > 46(5): 337-343. > DOI: 10.13386/j.issn1002-0306.2023090192

CHEN Zhixian, SHI Lei, ZHANG Yan. Effects of Lactobacillus acidophilus LA15 on Immune Enhancement and Regulation of Gut Microbiota in Mice[J]. Science and Technology of Food Industry, 2025, 46(5): 337−343. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090192.

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Effects of Lactobacillus acidophilus LA15 on Immune Enhancement and Regulation of Gut Microbiota in Mice

CHEN Zhixian^{1, 2, 3,},
SHI Lei^{1, 2, 3},
ZHANG Yan^{1, 2, 3, ,}

1.
National Key Laboratory of Agricultural Microbiology, Yichang 443003, China
2.
The Hubei Provincial Key Laboratory of Yeast Function, Yichang 443003, China
3.
Yi Chang Engineering and Technology Research Center of Nutrition and Health Food, Yichang 443003, China

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Received Date: September 19, 2023
Available Online: January 02, 2025

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Abstract

Abstract

The purpose of this study was to investigate the enhancing effect of Lactobacillus acidophilus LA15 on cyclophosphamide (CTX)-induced immunosuppression in BALB/c mice. The in vivo immunosuppression model was established by administering CTX through injection, and the immunomodulatory effects were assessed by measuring murine phenotypic indicators, immune markers, and gut microbiota. The results indicated that after induction by CTX, mice in the CTX group exhibited decreased body weight, thymus index, spleen index, immunoglobulin levels, and cytokine content. In contrast, LA15 significantly enhanced the thymus index, spleen index (P<0.05), relieve the swelling and inflammatory injury of small intestinal. The intestinal immunoglobulin A (sIgA) content in the LA15 group was 486.39 μg/mL, which was significantly higher than that in the model control group (294.72 μg/mL) (P<0.01), and the levels of TNF-α, IL-6, IL-10, IL-1β, IL-17 increased. 16S rDNA gene sequencing revealed that LA15 reduced the ratio of Firmicutes/Bacteroidetes, increased the relative abundance of Alloprevotella and Lachnospiraceae, and decreased the relative abundance of Desulfurvibrio. Therefore, LA15 could alleviate CTX-induced immunosuppression, reshape the gut microbiota, and was considered a potential immunostimulant for the treatment of immune suppression disorders.
- Lactobacillus acidophilus,
- cyclophosphamide,
- immunosuppression,
- cytokine,
- intestinal flora

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References

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