Differential Analysis of Five Chinese Fresh Peach Metabolites Based on Non-target Metabolomics

GU Ye; YANG Cheng; TANG Chao; HUAN Yu; MIAO Xiong

doi:10.13386/j.issn1002-0306.2023090132

Volume 45 Issue 17

Aug. 2024

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Science and Technology of Food Industry > 2024 > 45(17): 262-272. > DOI: 10.13386/j.issn1002-0306.2023090132

GU Ye, YANG Cheng, TANG Chao, et al. Differential Analysis of Five Chinese Fresh Peach Metabolites Based on Non-target Metabolomics[J]. Science and Technology of Food Industry, 2024, 45(17): 262−272. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090132.

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Differential Analysis of Five Chinese Fresh Peach Metabolites Based on Non-target Metabolomics

1.
Wuxi Food Safety Inspection and Test Center, Technology Innovation Center of Special Food for State Market Regulation, Wuxi 214142, China
2.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
3.
College of Engineering, University of Michigan, Ann Arbor 48109, U.S.
4.
SCIEX Analytical Instrument Trading Co., Ltd., Shanghai 200335, China

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Received Date: September 13, 2023
Available Online: July 04, 2024

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Objective: To investigate the differences in metabolites of Chinese nectarine, peento, yellow peach, Yangshan honey peach and Xinyi honey peach by using non-target metabolomics. Methods: 250 Fresh peaches of comparable maturity were used as test materials, and the fresh peach pulp was determined by high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry (UPLC-QTOF-MS), and the metabolites of different varieties of fresh peaches were investigated by principal component analysis and metabolite variability analysis. Results: A total of 74 metabolites were detected, mainly phenolic acids, flavanols, flavonols, anthocyanins, amino acids, glycosides, iridoid glycosides, B vitamins, etc. PCA analysis revealed that compared with the other 4 fresh peaches, Yangshan honey peach had higher contents of 7-hydroxycoumarin, pinocembrin, chlorogenic acid, neochlorogenic acid, epicatechin, procyanidin A₂, cryptochlorogenic acid, plantagoside, procyanidin B₂, and lower levels of vitamin B₂, kaempferol, rutin. Based on OPLS-DA analysis, 4 fresh peaches were screened for differential metabolites with Yangshan honey peach respectively. Nectarine had 18 species of procyanidin B₃ and other metabolites, peento had 12 species of procyanidin B₂ and other metabolites, yellow peach had 18 species of procyanidin A₂ and other metabolites, and Xinyi honey peach had 15 species of procyanidin C₁ and other metabolites, which could be used as a potential biomarker to differentiate between Yangshan honey peach and the other 4 fresh peaches. In this study, the differential metabolites of the 5 fresh peaches were mainly plant polyphenolic compounds, and chlorogenic acid and procyanidin metabolites were high in Yangshan honey peach, which were the key differential metabolites. Conclusion: The non-target metabolomics technology can effectively distinguish the five fresh peaches, which is feasible for the identification of fresh peaches, and can be used to assist the qualitative identification of fresh peaches and deep-processed fresh peaches as an important basis for the identification of true and false Yangshan honey peaches, and it is of great significance to protect the development of Yangshan honey peach speciality industry.
- fresh peach,
- metabolomics,
- UPLC-QTOF-MS,
- identification

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