Isolation, Purification, Structure Characterization and Antibacterial Activity of Polysaccharides from Proso Millet Bran

CHEN Yanyun; PING Hua; GAO Yuan; DU Yuanfang; LIU Jing; ZHANG Lizhen

doi:10.13386/j.issn1002-0306.2023090118

Volume 45 Issue 10

May 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(10): 1-7. > DOI: 10.13386/j.issn1002-0306.2023090118

CHEN Yanyun, PING Hua, GAO Yuan, et al. Isolation, Purification, Structure Characterization and Antibacterial Activity of Polysaccharides from Proso Millet Bran[J]. Science and Technology of Food Industry, 2024, 45(10): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090118.

Citation:

PDF (3733 KB)

Isolation, Purification, Structure Characterization and Antibacterial Activity of Polysaccharides from Proso Millet Bran

1.
School of Life Sciences, Shanxi University, Taiyuan 030006, China
2.
Institute of Quality Standard and Testing Technology of Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097, China

More Information

Received Date: September 12, 2023
Available Online: March 19, 2024

Graphical Abstract

Abstract

Abstract

The extraction, isolation, purification, and structural characterization of proso millet bran polysaccharides (MBP), as well as its antibacterial activity were conducted to provide a reference for the development and utilization of nutrients in the processing byproducts of millet. An alkali extraction and alcohol precipitation method was used to extract the polysaccharides. Subsequent purification was achieved through column chromatography utilizing DEAE-50 and Sephadex G-100. Structural analysis of the millet bran polysaccharides was performed using a combination of techniques, including HPLC chromatography, infrared spectroscopy, atomic force microscopy, scanning electron microscopy, and X-ray diffraction. The results showed that the molecular weight of MBP was determined to be 3.479×10⁴ Da. The monosaccharide composition was exhibited a molar ratio of mannose:rhamnose:glucose:galactose:arabinose:xylose=0.11:0.13:5.86:0.62:1.00:0.52. Moreover, MBP was identified as a β-type pyranose polysaccharide and exhibits remarkable thermal stability. In terms of morphology, MBP was laminated, closely connected, and had no crystal structure. The antibacterial assay unveiled the minimum inhibitory concentration of MBP against Staphylococcus aureus was 2 mg/mL. This study showed that the polysaccharides from proso millet bran had good antibacterial ability.
- proso millet bran,
- polysaccharide,
- separation purification,
- structural characterization,
- bacteriostatic activity

FullText(HTML)

References (32)

References

[1]	臧盛. 糜子壳多酚类物质抗氧化活性研究[D]. 咸阳:西北农林科技大学, 2010. [ZANG Sheng. Antioxidant activity of chylus shell polyphenols[D]. Xianyang:Northwest A&F University, 2010.] ZANG Sheng. Antioxidant activity of chylus shell polyphenols[D]. Xianyang: Northwest A&F University, 2010.
[2]	颜飞翔, 朱丹, 苗欣月, 等. 糜子麸皮中多酚提取工艺优化及其抗氧化活性[J]. 中国粮油学报,2020,35(12):15−22. [YAN Feixiang, ZHU Dan, MIAO Xinyue, et al. Optimization of polyphenol extraction process and its antioxidant activity in glutinous millet bran[J]. Chinese Journal of Grain and Oil,2020,35(12):15−22.] YAN Feixiang, ZHU Dan, MIAO Xinyue, et al. Optimization of polyphenol extraction process and its antioxidant activity in glutinous millet bran[J]. Chinese Journal of Grain and Oil, 2020, 35（12）: 15−22.
[3]	石松业, 温纪平, 刘远晓. 小麦麸皮多糖提取、结构及生物活性研究进展[J]. 食品工业科技,2023,44(13):466−473. [SHI Songye, WEN Jiping, LIU Yuanxiao. Research progress on the extraction, structure and biological activity of wheat bran polysaccharide[J]. Food Industry Technology,2023,44(13):466−473.] SHI Songye, WEN Jiping, LIU Yuanxiao. Research progress on the extraction, structure and biological activity of wheat bran polysaccharide[J]. Food Industry Technology, 2023, 44（13）: 466−473.
[4]	李佳妮, 白宝清, 金晓第, 等. 酶解超声波协同提取藜麦多糖及体外活性评价[J]. 食品研究与开发,2019,40(8):57−64. [LI Jiani, BAI Baoqing, JIN Xiaodi, et al. Enzymatic ultrasound synergistic extraction of quinoa polysaccharide and in vitro activity evaluation[J]. Food Research and Development,2019,40(8):57−64.] LI Jiani, BAI Baoqing, JIN Xiaodi, et al. Enzymatic ultrasound synergistic extraction of quinoa polysaccharide and in vitro activity evaluation[J]. Food Research and Development, 2019, 40（8）: 57−64.
[5]	ZHAO L P, ZHANG F, DING X Y, et al. Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes[J]. Science,2018,359(6380):1151−1156. doi: 10.1126/science.aao5774
[6]	SARMA S M, SINGH D P, SINGH P, et al. Finger millet arabinoxylan protects mice from high-fat diet induced lipid derangements, inflammation, endotoxemia and gut bacterial dysbiosis[J]. International Journal of Biological Macromolecules,2018,106:994−1003. doi: 10.1016/j.ijbiomac.2017.08.100
[7]	HUY C, ZHANG J M, ZOU L, et al, Chemical characterization, antioxidant, immune-regulating and anticancer activities of a novel bioactive polysaccharide from Chenopodium quinoa seeds[J]. International Journal of Biological Macromolecules, 2017, 99:622−629.
[8]	李杰, 田思雨, 谭怡然, 等. 山楂叶多糖提取工艺优化及体外抗氧化和抑菌活性研究[J]. 食品工业,2023,44(4):91−95. [LI Jie, TIAN Siyu, TAN Yiran, et al. Optimization of hawthorn leaf polysaccharide extraction process and in vitro antioxidant and antibacterial activity study[J]. Food Industry,2023,44(4):91−95.] LI Jie, TIAN Siyu, TAN Yiran, et al. Optimization of hawthorn leaf polysaccharide extraction process and in vitro antioxidant and antibacterial activity study[J]. Food Industry, 2023, 44（4）: 91−95.
[9]	杨宗芬, 王敏, 王贞利, 等. 冰菜多糖提取物的抗氧化性及抑菌活性研究[J]. 天津农学院学报,2023,30(1):1−5. [YANG Zongfen, WANG Min, WANG Zhenli, et al. Study on oxidative properties and antibacterial activity of iced polysaccharide extract[J]. Journal of Tianjin University of Agriculture,2023,30(1):1−5.] YANG Zongfen, WANG Min, WANG Zhenli, et al. Study on oxidative properties and antibacterial activity of iced polysaccharide extract[J]. Journal of Tianjin University of Agriculture, 2023, 30（1）: 1−5.
[10]	SPNOD, VHFMD, SEBASTIAO T, et al. Bacillus subtilis and Bacillus licheniformis promote tomato growth[J]. Brazilian journal of microbiology:[publication of the Brazilian Society for Microbiology], 2022, 54 (1):397-406.
[11]	张彤, 赵芸, 黄伟, 等. 天然多糖抑菌活性及机理研究进展[J]. 湖北农业科学, 2022, 61(11):158-162,169. [ZHANG Tong, ZHAO Yun, HUANG Wei, et al. Progress in the antibacterial activity and mechanism of natural polysaccharides[J]. Hubei Agricultural Science, 2022, 61 (11):158-162,169.] ZHANG Tong, ZHAO Yun, HUANG Wei, et al. Progress in the antibacterial activity and mechanism of natural polysaccharides[J]. Hubei Agricultural Science, 2022, 61 （11）: 158-162,169.
[12]	陈树俊, 崔云. 甘薯渣多糖提取、分离纯化及抗氧化活性研究[J]. 中国粮油学报,2020,35(10):56−62. [CHEN Shujun, CUI Yun. The polysaccharide extraction, purification and antioxidant activity of sweet potato residue[J]. Chinese Journal of Grain and Oil,2020,35(10):56−62.] CHEN Shujun, CUI Yun. The polysaccharide extraction, purification and antioxidant activity of sweet potato residue[J]. Chinese Journal of Grain and Oil, 2020, 35（10）: 56−62.
[13]	倪茂君, 张晓彬, 王静霞, 等. 黄芪多糖辐照预处理提取工艺及活性研究[J]. 核农学报,2023,37(9):1790−1797. [NI Maojun, ZHANG Xiaobin, WANG Jingxia, et al. Extraction process and activity of APS by irradiation[J]. Journal of Nuclear Agriculture,2023,37(9):1790−1797.] NI Maojun, ZHANG Xiaobin, WANG Jingxia, et al. Extraction process and activity of APS by irradiation[J]. Journal of Nuclear Agriculture, 2023, 37（9）: 1790−1797.
[14]	LIANG Xiaoxia, GAO Yingying, PAN Yan, et al. Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobiif[J]. Carbohydrate Polymers, 2019, 203(1):45−51.
[15]	刘露, 侯怡铃, 王梅, 等. “南薯88”茎叶多糖的分离纯化, 结构鉴定及其生物活性的研究[J]. 食品与生物技术学报,2019,38(2):101−110. [LIU Lu, HOU Yiling, WANG Mei, et al. Isolation and purification of stem and leaf polysaccharides of "Southern potato 88", structural identification and its biological activity[J]. Journal of Food and Biotechnology,2019,38(2):101−110.] LIU Lu, HOU Yiling, WANG Mei, et al. Isolation and purification of stem and leaf polysaccharides of "Southern potato 88", structural identification and its biological activity[J]. Journal of Food and Biotechnology, 2019, 38（2）: 101−110.
[16]	李占君, 张厚良, 范瑞红, 等. 云南林下塔拉多糖的红外光谱、分子量和衍射图谱分析研究[J]. 林业科技,2018,43(3):49−52. [LI Zhanjun, ZHANG Houliang, FAN Ruihong, et al. Infrared spectroscopy, molecular weight and diffraction mapping of tarara polysaccharide in Yunnan[J]. Forestry Science and Technology,2018,43(3):49−52.] doi: 10.3969/j.issn.1001-9499.2018.03.013 LI Zhanjun, ZHANG Houliang, FAN Ruihong, et al. Infrared spectroscopy, molecular weight and diffraction mapping of tarara polysaccharide in Yunnan[J]. Forestry Science and Technology, 2018, 43（3）: 49−52. doi: 10.3969/j.issn.1001-9499.2018.03.013
[17]	杨许花, 张竞文, 刘红海, 等. 藏柳茶多糖的提取、结构解析及抗氧化活性分析[J]. 现代食品科技,2022,38(12):318−328. [YANG Xuhua, ZHANG Jingwen, LIU Honghai, et al. Extraction, structure analysis and antioxidant activity analysis of Tibetan willow tea polysaccharide[J]. Modern Food Technology,2022,38(12):318−328.] YANG Xuhua, ZHANG Jingwen, LIU Honghai, et al. Extraction, structure analysis and antioxidant activity analysis of Tibetan willow tea polysaccharide[J]. Modern Food Technology, 2022, 38（12）: 318−328.
[18]	梁涛, 张静, 张力妮, 等. 碱提杏鲍菇多糖PEAP-1的结构初探及形貌观察[J]. 食品与生物技术学报,2013,32(9):951−956. [LIANG Tao, ZHANG Jing, ZHANG Lini, et al. The structure and morphology of PEAP-1[J]. Journal of Food and Biotechnology,2013,32(9):951−956.] LIANG Tao, ZHANG Jing, ZHANG Lini, et al. The structure and morphology of PEAP-1[J]. Journal of Food and Biotechnology, 2013, 32（9）: 951−956.
[19]	马永强, 张一鹏, 王鑫, 等. 九蒸九制对黄精中AGEs含量、多糖结构及体外活性的影响[J]. 食品工业科技,2024,45(3):226-234. [MA Y Q, ZHANG Y P, WANG X, et al. Effect of nine steaming nine system on AGEs content, polysaccharide structure and in vitro activity in yellow essence [J]. Food Industry Science and Technology,2024,45(3):226-234.] MA Y Q, ZHANG Y P, WANG X, et al. Effect of nine steaming nine system on AGEs content, polysaccharide structure and in vitro activity in yellow essence [J]. Food Industry Science and Technology, 2024, 45（3）: 226-234.
[20]	周欣. 香菇多糖提取工艺的优化及其体外抗菌试验研究[J]. 饲料研究, 2023(13):87−92. [ZHOU Xin. Optimization of lentinpolysaccharide extra-ction process and in vitro antimicrobial test[J]. Feed Study, 2023 (13):87−92.] ZHOU Xin. Optimization of lentinpolysaccharide extra-ction process and in vitro antimicrobial test[J]. Feed Study, 2023 （13）: 87−92.
[21]	岳明, 阿迪拉·阿布都热西提, 尼格尔热依·亚迪卡尔, 等. 洋甘菊残渣抑菌活性及作用研究[J]. 食品研究与开发,2023,44(4):36−42. [YUE M, ADILA A R, NIGEL D, et al. Study on the antibacterial activity and function of chamomile residue[J]. Food Research and Development,2023,44(4):36−42.] doi: 10.12161/j.issn.1005-6521.2023.04.006 YUE M, ADILA A R, NIGEL D, et al. Study on the antibacterial activity and function of chamomile residue[J]. Food Research and Development, 2023, 44（4）: 36−42. doi: 10.12161/j.issn.1005-6521.2023.04.006
[22]	SHANG Xiaolan, LIU Chunyu, DONG Haiyan, et al. Extraction, purification, structural characterization, and antioxidant activity of polysaccharides from wheat bran[J]. Journal of Molecular Structure, 2021, 1233.
[23]	CAO Jingjing, TANG Dandan, WANG Yue, et al. Characteristics and immuneenhancing activity of pectic polysaccharides from sweet cherry (Prunus avium)[J]. Food Chemistry,2018,254:47−54. doi: 10.1016/j.foodchem.2018.01.145
[24]	JI Xiaolong, LIU Fang, PENG Qiang, et al. Purification, structural characterization, and hypolipidemic effects of a neutral polysaccharide from Ziziphus jujuba cv. Muzao[J]. Food Chemistry,2018,245:1124−1130. doi: 10.1016/j.foodchem.2017.11.058
[25]	ZHENG Yefeng, ZHANG Shuai, WANG Qi, et al. Characterization and hypoglycemic activity of a ß-pyran polysaccharides from bamboo shoot (Leleba oldhami Nakal) shells[J]. Carbohydrate Polymers,2016,144:438−446. doi: 10.1016/j.carbpol.2016.02.073
[26]	REN Y P, LIU S X. Effects of separation and purification on structural characteristics of polysaccharide from quinoa (Chenopodium quinoa Willd)[J]. Biochemical and Biophysical Research Communications,2020,522(2):286−291. doi: 10.1016/j.bbrc.2019.10.030
[27]	ZHANG Xuyu, LIU Zijing, ZHONG Cheng, et al. Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from Radix Ginseng Rubra[J]. International Journal of Biological Macromolecules,2021,189:980−992. doi: 10.1016/j.ijbiomac.2021.08.176
[28]	WANG Shengnan, QU Danni, ZHAO Guilan, et al. Characterization of the structure and properties of the isolating interfacial layer of oil-water emulsions stabilized by soy hull polysaccharide:Effect of pH changes[J]. Food Chemistry,2022,370:131029. doi: 10.1016/j.foodchem.2021.131029
[29]	王鑫, 陈什康, 张婷, 等. 小麦麸皮粗多糖的基本结构特征、流变特性及其对体外消化酶抑制初探[J]. 南昌大学学报(理科版),2022,46(3):334−341. [WANG Xin, CHEN Shikang, ZHANG Ting, et al. Basic structural features, rheological properties of wheat bran crude polysaccharides and their inhibition of digestive enzymes in vitro[J]. Journal of Nanchang University (Science Edition),2022,46(3):334−341.] doi: 10.3969/j.issn.1006-0464.2022.03.008 WANG Xin, CHEN Shikang, ZHANG Ting, et al. Basic structural features, rheological properties of wheat bran crude polysaccharides and their inhibition of digestive enzymes in vitro[J]. Journal of Nanchang University （Science Edition）, 2022, 46（3）: 334−341. doi: 10.3969/j.issn.1006-0464.2022.03.008
[30]	LI Wei, ZHANG Yanqing, SANG Lintao, et al. Effects of different extraction techniques on the structural, physicochemical, and bioactivity properties of heteropolysaccharides from Platycodon grandiflorum roots[J]. Process Biochemistry, 2023, 127, 33-43.
[31]	别蒙, 谢笔钧, 孙智达. 不同取代度水溶性羧甲基茯苓多糖的制备、结构表征及体外抑菌活性[J]. 食品科学,2020,41(12):67−76. [BIE Meng, XIE Bijun, SUN Zhida. Preparation, structural characterization and in vitro antibacterial activity of water-soluble carboxymethyl poria polysaccharide with different substitutions[J]. [J]. Food Science,2020,41(12):67−76.] doi: 10.7506/spkx1002-6630-20190603-015 BIE Meng, XIE Bijun, SUN Zhida. Preparation, structural characterization and in vitro antibacterial activity of water-soluble carboxymethyl poria polysaccharide with different substitutions[J]. [J]. Food Science, 2020, 41（12）: 67−76. doi: 10.7506/spkx1002-6630-20190603-015
[32]	魏磊, 王伟, 谢晓阳, 等. 响应面优化博爱赤松茸多糖提取工艺及其抑菌和抗氧化活性研究[J]. 食品工业科技,2023,44(15):213−220. [WEI Lei, WANG Wei, XIE Xiaoyang, et al. Optimization of the polysaccharide extraction process and its antibacterial and antioxidant activity[J]. Food Industry Technology,2023,44(15):213−220.] WEI Lei, WANG Wei, XIE Xiaoyang, et al. Optimization of the polysaccharide extraction process and its antibacterial and antioxidant activity[J]. Food Industry Technology, 2023, 44（15）: 213−220.

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(7)

1.	郭慧慧，蒋元斌，林丛发，徐绍翔，林泽宇，薛立云. 太子参脱毒苗培养、化学成分及指纹图谱研究进展. 药学研究. 2024(03): 274-281 .
2.	张森，欧婧，豆晓霞，刘晓东，付本懂. 太子参及提取物对动物免疫调节作用研究进展. 动物医学进展. 2024(05): 97-102 .
3.	张春雨，邢鹏，周福荣，肖逸豪，赵红兵. 中药复方养肝活血汤对酒精性肝病的临床研究. 中国民族医药杂志. 2024(09): 11-14 .
4.	倪建成，范永飞，叶祖云. 太子参化学成分、药理作用和应用的研究进展. 中草药. 2023(06): 1963-1977 .
5.	游绍伟，詹亚梅，王文素，何典城，蓬兴柱，王学勇. 基于“脾虚宛滞”探讨慢性萎缩性胃炎“炎癌转化”与防治思路. 中国实验方剂学杂志. 2023(21): 188-195 .
6.	文丁苑，梁双敏，国琦，宋晓晓，葛长荣，肖智超. 榆黄菇多糖提取工艺优化及其免疫调节活性评价. 现代食品科技. 2023(10): 233-243 .
7.	谢雄雄，孟璞岩，朱灵芝，陈宜均，龚斌，李康琴，邓绍勇. 太子参的药理活性、化学成分及繁殖栽培研究进展. 南方林业科学. 2023(05): 60-64+78 .