Application of Time-resolved Fluorescence Immunoassay to Concurrently Quantify Fumonisin B1, B2 and B3 in Corn

ZHANG Jialin; MA Licai; HOU Lu; Menghenaren; WEN Kai; HOU Xiaolin

doi:10.13386/j.issn1002-0306.2023080334

Volume 45 Issue 13

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(13): 265-271. > DOI: 10.13386/j.issn1002-0306.2023080334

ZHANG Jialin, MA Licai, HOU Lu, et al. Application of Time-resolved Fluorescence Immunoassay to Concurrently Quantify Fumonisin B₁, B₂ and B₃ in Corn[J]. Science and Technology of Food Industry, 2024, 45(13): 265−271. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080334.

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Application of Time-resolved Fluorescence Immunoassay to Concurrently Quantify Fumonisin B₁, B₂ and B₃ in Corn

ZHANG Jialin^{1, 2,},
MA Licai²,
HOU Lu¹,
Menghenaren³,
WEN Kai⁴,
HOU Xiaolin^1, ,

1.
College of Animal Science and Technology, Beijing University of Agricultural, Beijing 102206, China
2.
Beijing WDWK Biotechnology Co., Ltd., Beijing 100095, China
3.
Chifeng City Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement Detachment, Chifeng 024000, China
4.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

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Received Date: September 03, 2023
Available Online: May 04, 2024

Graphical Abstract

Abstract

Abstract

This study aimed to establish a time-resolved fluorescence immunoassay method for the simultaneous quantitative detection of fumonisin B₁, B₂ and B₃ in corn. The EDC/NHS active ester method was used to prepare time-resolved fluorescent microspheres and fumonisin antibody conjugates. Fumonisin antigen was immobilized on a nitrocellulose membrane as the test line. By optimizing the number of test line, a competitive immunoassay method for detecting fumonisin was eventually established. Subsequently, the sensitivity, specificity, accuracy, precision, and correlation with the national standard method were evaluated for the established method. The detection limits of the established method by using two test line pattern were 107.68~168.28 μg/kg, and the quantification limits were 283.46~444.63 μg/kg. The cross-reactivity with fumonisin B₁, fumonisin B₂ and fumonisin B₃ were 100%, 85.59% and 72.72%, respectively, and no significant cross-reactivity was observed with five other common mycotoxins. The recovery rates ranged from 88.37% to 117.42%, and the coefficients of variation were lower than 10%. The conformity of the results obtained by the established method, as compared to the immunoaffinity column purification-high performance liquid chromatography (IAC-HPLC) method specified in the national standard GB 5009.240-2016, fell within the range of 92.17% to 107.21%. The established time-resolved fluorescence immunoassay method meets the requirements for rapid on-site quantitative detection of fumonisin B₁, B₂ and B₃ in corn.
- time-resolved fluorescent microsphere,
- fumonisin B₁,
- fumonisin B₂,
- fumonisin B₃,
- corn,
- quantitative detection

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References (31)

References

[1]	FARHADI A, FAKHRI Y, KACHUEI R, et al. Prevalence and concentration of fumonisins in cereal-based foods:A global systematic review and meta-analysis study[J]. Environmental Science and Pollution Research,2021,28(17):20998−21008. doi: 10.1007/s11356-021-12671-w
[2]	BRYLA M, WASKIEWICZ A, SZYMCZYK K, et al. Effects of pH and temperature on the stability of fumonisins in maize products[J]. Toxins,2017,9(3):2−16.
[3]	丁燕玲, 李孟聪, 钟名琴, 等. 2015-2020年国内饲料霉菌毒素污染调查报告统计分析[J]. 中国动物检疫,2021,38(3):29−36. [DING Yanling, LI Mengcong, ZHONG Mingqin, et al. Statistical analysis of domestic feed mycotoxin contamination investigation reports from 2015 to 2020[J]. Chinese Journal of Animal Quarantine,2021,38(3):29−36.] DING Yanling, LI Mengcong, ZHONG Mingqin, et al. Statistical analysis of domestic feed mycotoxin contamination investigation reports from 2015 to 2020[J]. Chinese Journal of Animal Quarantine, 2021, 38（3）: 29−36.
[4]	杨晓冬, 赵凤, 罗靖, 等. 2022年四川省三地区谷物及制品真菌污染现状调查[J]. 预防医学情报,2023,39(7):830−834. [YANG Xiaodong, ZHAO Feng, LUO Jing, et al. Investigation of fungal contamination status in grains and grain products in three regions of Sichuan Province in 2022[J]. Journal of Preventive Medicine Information,2023,39(7):830−834.] YANG Xiaodong, ZHAO Feng, LUO Jing, et al. Investigation of fungal contamination status in grains and grain products in three regions of Sichuan Province in 2022[J]. Journal of Preventive Medicine Information, 2023, 39（7）: 830−834.
[5]	SOUSA F C, SCHAMBER C R, AMORIN S S, et al. Effect of fumonisin containing diet on the myenteric plexus of the jejunum in rats[J]. Autonomic Neuroscience Basic & Clinical,2014,185(15):93−99.
[6]	GRENIER B, BRACARENSE A P, SCHWARTZ H E, et al. The low intestinal and hepatic toxicity of hydrolyzed fumonisin B correlates with its inability to alter the metabolism of sphingolipids[J]. Biochemical Pharmacology,2012,83(10):1465−1473. doi: 10.1016/j.bcp.2012.02.007
[7]	TARANU I, MARIN D E, BOUHET S, et al. Mycotoxin fumonisin B₁ alters the cytokine profile and decreases the vaccinal antibody titer in pigs[J]. Toxicological Sciences,2005,84(2):301−307. doi: 10.1093/toxsci/kfi086
[8]	YOSHIZAWA T, YAMASHITA A, LUO Y. Fumonisin occurrence in corn from high- and low-risk areas for human esophageal cancer in China[J]. Applied & Environmental Microbiology,1994,60(5):1626−1629.
[9]	尚艳娥, 杨卫民. CAC、欧盟、美国与中国粮食中真菌毒素限量标准的差异分析[J]. 食品科学技术学报,2019,37(1):10−15. [SHANG Yane, YANG Weimin. Comparative analysis of limits for mycotoxins in food between Codex Alimentarius Commission, the European Union, the United States, and China[J]. Journal of Food Science and Technology,2019,37(1):10−15.] SHANG Yane, YANG Weimin. Comparative analysis of limits for mycotoxins in food between Codex Alimentarius Commission, the European Union, the United States, and China[J]. Journal of Food Science and Technology, 2019, 37（1）: 10−15.
[10]	中华人民共和国国家质量监督检验检疫总局. GB13078-2017饲料卫生标准[S]. 北京:中国标准出版社, 2017. [General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. GB13078-2017 Feed hygiene standards[S]. Beijing:China Standards Press, 2017.] General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. GB13078-2017 Feed hygiene standards[S]. Beijing: China Standards Press, 2017.
[11]	李寒松, 樊海新, 李复辉, 等. 伏马菌素B₁的毒性、污染及其检测技术[J]. 黑龙江畜牧兽医,2016(3):275−278. [LI Hansong, FAN Haixin, LI Fuhui, et al. Toxicity, contamination, and detection techniques of fumonisin B₁[J]. Heilongjiang Animal Science and Veterinary Medicine,2016(3):275−278.] LI Hansong, FAN Haixin, LI Fuhui, et al. Toxicity, contamination, and detection techniques of fumonisin B₁[J]. Heilongjiang Animal Science and Veterinary Medicine, 2016（3）: 275−278.
[12]	SMITH L L, FRANCIS K A, JOHNSON J T, et al. Quantitation of fumonisin B₁ and B₂ in feed using FMOC pre-column derivatization with HPLC and fluorescence detection[J]. Food Chemistry,2017,234:174−179. doi: 10.1016/j.foodchem.2017.04.142
[13]	ZHANG S, ZHOU S, YU S, et al. LC-MS/MS analysis of fumonisin B₁, B₂, B₃, and their hydrolyzed metabolites in broiler chicken feed and excreta[J]. Toxins,2022,14(2):131. doi: 10.3390/toxins14020131
[14]	李莉, 李硕. QuEChERS-超高效液相色谱-串联质谱法测定玉米油中伏马毒素B₁、B₂、B₃[J]. 食品安全质量检测学报,2020,11(19):7006−7011. [LI Li, LI Shuo. Determination of fumonisins B₁, B₂, and B₃ in corn oil by QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality Inspection,2020,11(19):7006−7011.] LI Li, LI Shuo. Determination of fumonisins B₁, B₂, and B₃ in corn oil by QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality Inspection, 2020, 11（19）: 7006−7011.
[15]	刘师文, 何庆华, 邹龙, 等. 谷物中伏马菌素B₁酶联免疫分析法的建立[J]. 食品科学,2010,31(18):350−354. [LIU Shiwen, HE Qinghua, ZOU Long, et al. Establishment of an enzyme-linked immunosorbent assay for fumonisin B₁ in cereals[J]. Food Science,2010,31(18):350−354.] LIU Shiwen, HE Qinghua, ZOU Long, et al. Establishment of an enzyme-linked immunosorbent assay for fumonisin B₁ in cereals[J]. Food Science, 2010, 31（18）: 350−354.
[16]	任文洁, 黄志兵, 许杨, 等. 伏马菌素B₁胶体金免疫层析快速检测试纸条的研制[J]. 食品工业科技,2015,36(24):58−63. [REN Wenjie, HUANG Zhibing, XU Yang, et al. Development of colloidal gold immunochromatographic rapid test strip for fumonisin B₁[J]. Food Industry Science and Technology,2015,36(24):58−63.] REN Wenjie, HUANG Zhibing, XU Yang, et al. Development of colloidal gold immunochromatographic rapid test strip for fumonisin B₁[J]. Food Industry Science and Technology, 2015, 36（24）: 58−63.
[17]	谢婷婷. 粮食中伏马毒素胶体金快速定量法研究[J]. 粮食与饲料工业,2019,9(15):60−63. [XIE Tingting. Research on colloidal gold rapid quantitative method for fumonisins in grains[J]. Cereal and Feed Industry,2019,9(15):60−63.] XIE Tingting. Research on colloidal gold rapid quantitative method for fumonisins in grains[J]. Cereal and Feed Industry, 2019, 9（15）: 60−63.
[18]	DEGAN P. Time-resolved fluoroimmuno assay[J]. Molecular Biotechnology,2000,13(3):215−222.
[19]	刘洪美, 郑梦瑶, 袁华山, 等. 基于时间分辨荧光纳米微球的伏马毒素B₁快速定量检测卡的制备及应用[J]. 粮油食品科技,2022,30(4):164−171. [LIU Hongmei, ZHENG Mengyao, YUAN Huashan, et al. Preparation and application of a rapid quantitative detection card for fumonisin B₁ based on time-resolved fluorescence nanospheres[J]. Cereal and Oil Food Science and Technology,2022,30(4):164−171.] LIU Hongmei, ZHENG Mengyao, YUAN Huashan, et al. Preparation and application of a rapid quantitative detection card for fumonisin B₁ based on time-resolved fluorescence nanospheres[J]. Cereal and Oil Food Science and Technology, 2022, 30（4）: 164−171.
[20]	陈建玲, 李文学, 董志宁, 等. 伏马毒素B₁时间分辨免疫荧光检测试剂盒的研制[J]. 癌变畸变突变,2012,25(1):48−52. [CHEN Jianling, LI Wenxue, DONG Zhining, et al. Development of a time-resolved immunofluorescence detection kit for fumonisin B₁[J]. Carcinogenesis, Teratogenesis & Mutagenesis,2012,25(1):48−52.] CHEN Jianling, LI Wenxue, DONG Zhining, et al. Development of a time-resolved immunofluorescence detection kit for fumonisin B₁[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2012, 25（1）: 48−52.
[21]	张滢. 伏马毒素B₁和T-2毒素荧光免疫层析试纸条的研制[D]. 天津:天津科技大学, 2019. [ZHANG Ying. Development of fluorescent immunochromatographic test strips for fumonisin B₁ and T-2 toxin[D]. Tianjin:Tianjin University of Science and Technology, 2019.] ZHANG Ying. Development of fluorescent immunochromatographic test strips for fumonisin B₁ and T-2 toxin[D]. Tianjin: Tianjin University of Science and Technology, 2019.
[22]	叶金, 郑梦瑶, 马海华, 等. 基于时间分辨荧光快速定量检测谷物中的T-2毒素[J]. 中国粮油学报,2023,38(1):22−28. [YE Jin, ZHENG Mengyao, MA Haihua, et al. Rapid quantitative detection of T-2 toxin in cereals based on time-resolved fluorescence[J]. Chinese Journal of Cereals and Oils,2023,38(1):22−28.] doi: 10.3969/j.issn.1003-0174.2023.01.005 YE Jin, ZHENG Mengyao, MA Haihua, et al. Rapid quantitative detection of T-2 toxin in cereals based on time-resolved fluorescence[J]. Chinese Journal of Cereals and Oils, 2023, 38（1）: 22−28. doi: 10.3969/j.issn.1003-0174.2023.01.005
[23]	魏黛娴. 快速检测玉米和大豆中呕吐毒素的荧光免疫层析试纸条的研究[D]. 南昌:南昌大学, 2022. [WEI Daixian. Study on fluorescent immunochromatographic test strips for rapid detection of vomitoxin in corn and soybeans[D]. Nanchang:Nanchang University, 2022.] WEI Daixian. Study on fluorescent immunochromatographic test strips for rapid detection of vomitoxin in corn and soybeans[D]. Nanchang: Nanchang University, 2022.
[24]	丁亚芳, 贾良羲, 邢维维, 等. 基于免疫磁珠前处理的荧光免疫层析法定量检测牦牛肉中金刚烷胺[J]. 食品科技,2021,46(6):294−300. [DING Yafang, JIA Liangxi, XING Weiwei, et al. Quantitative detection of clenbuterol in yak meat based on immunomagnetic bead pretreatment and fluorescence immunoassay[J]. Food Science and Technology,2021,46(6):294−300.] DING Yafang, JIA Liangxi, XING Weiwei, et al. Quantitative detection of clenbuterol in yak meat based on immunomagnetic bead pretreatment and fluorescence immunoassay[J]. Food Science and Technology, 2021, 46（6）: 294−300.
[25]	赵维章, 崔乃元, 张汉青, 等. 时间分辨荧光免疫层析定量检测牦牛肉中喹诺酮类药物[J]. 食品工业科技,2020,41(18):239−251. [ZHAO Weizhang, CUI Naiyuan, ZHANG Hanqing, et al. Quantitative detection of quinolone residues in yak meat by time-resolved fluorescence immunoassay[J]. Food Industry Science and Technology,2020,41(18):239−251.] ZHAO Weizhang, CUI Naiyuan, ZHANG Hanqing, et al. Quantitative detection of quinolone residues in yak meat by time-resolved fluorescence immunoassay[J]. Food Industry Science and Technology, 2020, 41（18）: 239−251.
[26]	TANG Xiaolin, PIKAL M J. Design of freeze-drying processes for pharmaceuticals:Practical advice[J]. Pharmaceutical Research,2004,21(2):191−199. doi: 10.1023/B:PHAM.0000016234.73023.75
[27]	国家粮食局. LS/T 6402-2017 粮油检验设备和方法标准适用性验证及结果评价一般原则[S]. 北京:中国标准出版社, 2017. [State Administration of Grain. LS/T 6402-2017 General principles for applicability verification and result evaluation of equipment and methods standards for grain and oil inspection[S]. Beijing:China Standard Press, 2017.] State Administration of Grain. LS/T 6402-2017 General principles for applicability verification and result evaluation of equipment and methods standards for grain and oil inspection[S]. Beijing: China Standard Press, 2017.
[28]	中华人民共和国国家卫生和计划生育委员会. GB 5009.240-2016食品安全国家标准食品中伏马毒素的测定[S]. 北京:中国标准出版社, 2014. [National Health and Family Planning Commission of the People's Republic of China. GB 5009.240-2016 National food safety standard-determination of fumonisins in food[S]. Beijing:China Standards Press, 2014.] National Health and Family Planning Commission of the People's Republic of China. GB 5009.240-2016 National food safety standard-determination of fumonisins in food[S]. Beijing: China Standards Press, 2014.
[29]	巩性涛, 王培, 肖理文, 等. 呕吐毒素荧光定量快速检测试纸卡及仪器技术性能优化的研究报告[J]. 粮食加工,2021,46(2):84−91. [GONG Xingtao, WANG Pei, XIAO Wenli, et al. Research report on fluorescence quantitative rapid detection test card for vomitoxin and optimization of instrument technical performance[J]. Cereal Processing,2021,46(2):84−91.] GONG Xingtao, WANG Pei, XIAO Wenli, et al. Research report on fluorescence quantitative rapid detection test card for vomitoxin and optimization of instrument technical performance[J]. Cereal Processing, 2021, 46（2）: 84−91.
[30]	孔繁霞, 赵皖, 丁丽, 等. 基于时间分辨荧光微球的黄曲霉毒素B₁定量检测卡灵敏度的优化[J]. 粮食储藏,2022,51(3):40−44. [KONG Fanxia, ZHAO Wan, DING Li, et al. Optimization of sensitivity for quantitative detection card of aflatoxin B₁ based on time-resolved fluorescence microspheres[J]. Grain Storage,2022,51(3):40−44.] doi: 10.3969/j.issn.1000-6958.2022.03.008 KONG Fanxia, ZHAO Wan, DING Li, et al. Optimization of sensitivity for quantitative detection card of aflatoxin B₁ based on time-resolved fluorescence microspheres[J]. Grain Storage, 2022, 51（3）: 40−44. doi: 10.3969/j.issn.1000-6958.2022.03.008
[31]	江西省兽药饲料监察所. DB 36/T 1023-2018 饲料中伏马毒素的快速筛查胶体金快速定量法[S]. 南昌:江西省质量技术监督所, 2018. [Jiangxi Provincial Institute for the Control of Veterinary Drugs and Feeds. DB 36/T 1023-2018 Rapid screening of fumonisins in feeds-colloidal gold rapid quantitative method[S]. Nanchang:Jiangxi Provincial Institute of Quality and Technical Supervision, 2018.] Jiangxi Provincial Institute for the Control of Veterinary Drugs and Feeds. DB 36/T 1023-2018 Rapid screening of fumonisins in feeds-colloidal gold rapid quantitative method[S]. Nanchang: Jiangxi Provincial Institute of Quality and Technical Supervision, 2018.