Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of <i>Dendrobium officinale</i> Neutral Polysaccharide

LIN Xiaoliang; XIE Lingna; CHEN Mengxiang; LIANG Ming; LIANG Yiheng; DU Zhiyun

doi:10.13386/j.issn1002-0306.2023080137

Volume 45 Issue 8

Apr. 2024

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Science and Technology of Food Industry > 2024 > 45(8): 39-46. > DOI: 10.13386/j.issn1002-0306.2023080137

LIN Xiaoliang, XIE Lingna, CHEN Mengxiang, et al. Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide[J]. Science and Technology of Food Industry, 2024, 45(8): 39−46. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080137.

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Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide

1.
Infinity (China) Co., Ltd., Guangzhou 510623, China
2.
School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China

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Received Date: August 14, 2023
Available Online: February 25, 2024

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Abstract

Objective: To isolate and purify polysaccharides from Dendrobium officinale, characterize their structure and explore their anti-inflammatory effects. Methods: Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation. DOP-1 was purified by DEAE-52 cellulose ion exchange resin and Sephadex G-100. Ultraviolet scanning, GC, high-performance gel chromatography, infrared spectroscopy and nuclear magnetic resonance scanning were used to analyze the structure of DOP-1 polysaccharide, and the inhibitory effect of DOP-1 on the proliferation of RAW 264.7 cells was determined. And its effect on the expression of NO, TNF-α and IL-1β in RAW264.7 cells induced by LPS. Results: DOP-1 was a homogeneous polysaccharide composed of rhamnose, mannose, glucose and galactose with a monosaccharide molar ratio of 0.6:9.0:2.7:2.9 and a molecular weight of 59.2 kDa. DOP-1 did not significantly inhibit the proliferation of RAW 264.7 cells. Compared with the model group, DOP-1 significantly inhibited the expression of NO, TNF-α and IL-1β. Conclusions: The polysaccharides of Dendrobium officinaleobtained in this study had clear structure and good anti-inflammatory activity, and the extraction conditions were feasible. This study would provide theoretical basis for the development and utilization of polysaccharides of Dendrobium officinale with anti-inflammatory effect.
- Dendrobium officinale,
- neutral polysaccharide,
- isolation and purification,
- structural analysis,
- anti-inflammatory effect

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