Research Progress in Metabolic Engineering Strategies for Microbial Synthesis of 2′-Fucosyllactose and 3-Fucosyllactose

LI Dengbin; WEI Chao; ZHANG Yuan; ZHANG Genlin; WANG Xiaoyan

doi:10.13386/j.issn1002-0306.2023060216

Volume 45 Issue 10

May 2024

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Science and Technology of Food Industry > 2024 > 45(10): 376-385. > DOI: 10.13386/j.issn1002-0306.2023060216

LI Dengbin, WEI Chao, ZHANG Yuan, et al. Research Progress in Metabolic Engineering Strategies for Microbial Synthesis of 2′-Fucosyllactose and 3-Fucosyllactose[J]. Science and Technology of Food Industry, 2024, 45(10): 376−385. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060216.

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Research Progress in Metabolic Engineering Strategies for Microbial Synthesis of 2′-Fucosyllactose and 3-Fucosyllactose

LI Dengbin^1,,
WEI Chao^{2, 3, +,},
ZHANG Yuan^{2, 3},
ZHANG Genlin^1, ,,
WANG Xiaoyan^{2, 3, ,}

1.
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China
2.
Nutrition & Health Research Institute, China National Cereals, Oils and Foodstuffs Corporation (COFCO),Beijing 102209, China
3.
Beijing Key Laboratory of Nutrition, Health and Food Safety, Beijing 102209, China

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Received Date: June 24, 2023
Available Online: March 17, 2024

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Abstract

Abstract

Human milk oligosaccharides (HMOs) are key components of breast milk, playing a vital role in fostering the healthy development of infants and young children. Among the main HMOs components, 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) exhibit considerable benefits in terms of inhibiting pathogenic infections, regulating gut microbiota, and enhancing immunity. In recent years, the synthesis of 2′-FL and 3-FL has emerged as a prominent area of research in the field. Despite the potential advantages of chemical or enzymatic synthesis of 2′-FL and 3-FL, these methods are hindered by their inherent limitations, including intricate reaction procedures, high costs, and poor yield, which are not conducive to meeting the demand of the market. Therefore, more efficient microbial methods have been developed and applied in the commercial production of 2′-FL and 3-FL. This article systematically outlines the methods for preparing both 2′-FL and 3-FL, thoroughly expounds upon the engineering strategies for microbial synthesis of 2′-FL and 3-FL, and provides a compelling framework for further microbial production exploration of 2′-FL and 3-FL.
- 2′-fucosyllactose,
- 3-fucosyllactose,
- microbiological method,
- engineering strategy

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References (62)

References

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