Study on Gradient Alcohol Precipitation of Fucoidan from <i>Undaria pinnatifida</i> and Its Hypoglycemic Activity <i>in Vitro</i>

LI Can; LÜ Jinbo; LIU Huiping; TIAN Yue; ZHANG Sheng; LÜ Binfei; XUE Tianrui; ZHANG Mingran

doi:10.13386/j.issn1002-0306.2023060186

Volume 45 Issue 8

Apr. 2024

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Science and Technology of Food Industry > 2024 > 45(8): 309-317. > DOI: 10.13386/j.issn1002-0306.2023060186

LI Can, LÜ Jinbo, LIU Huiping, et al. Study on Gradient Alcohol Precipitation of Fucoidan from Undaria pinnatifida and Its Hypoglycemic Activity in Vitro[J]. Science and Technology of Food Industry, 2024, 45(8): 309−317. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060186.

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Study on Gradient Alcohol Precipitation of Fucoidan from Undaria pinnatifida and Its Hypoglycemic Activity in Vitro

College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China

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Received Date: June 19, 2023
Available Online: February 26, 2024

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Abstract

Abstract

To study the graded alcohol precipitation components of fucoidan from Undaria pinnatifida and its hypoglycemic activity in vitro, this study used Undaria pinnatifida as raw material, extracted Undaria pinnatifida fucoidan gum through CaCl₂ solution, and precipitated it step by step to obtain four sets of polysaccharide samples (UPP-45, UPP-55, UPP-65, and UPP-75). Determined their molecular weight, total sugar content, uronic acid content, protein content, sulfate content and monosaccharide composition and analyzed by ultraviolet spectrum (UV), Fourier transform infrared spectra(FT-IR) and scanning electron microscopy(SEM). The inhibition of polysaccharides on α-amylase and α-glucosidase was determined to explore their hypoglycemic activity in vitro. The results showed that the higher the concentration of alcohol precipitation, the higher the proportion of small molecular weight polysaccharide. The highest total sugar content of UPP-65 was 84.38%, and the protein of the crude polysaccharide and sulfate contents of UPP-45 were significantly different from those of other components (P<0.05). All the four polysaccharide components were acidic polysaccharides. The monosaccharide composition analysis showed that the monosaccharide composition and mole percent of polysaccharide components were different. The UV results showed that the content of nucleic acid of polysaccharide components was different. The FT-IR results showed that four components had different levels of absorption peaks of functional groups and glycosidic bonds. The SEM results showed that the microscopic morphology of polysaccharide components was different. The inhibition of α-amylase was not dose-dependent, and the inhibitory rate of UPP-65 was the highest with IC₅₀ value of 0.008 mg/mL. The inhibition of α-glucosidase showed a dose-effect relationship, and UPP-65 had the lowest IC₅₀ at 2.346 mg/mL. In summary, the fractional alcohol precipitation of Undaria pinnatifida polysaccharides technology was feasible. All the four polysaccharide components had certain hypoglycemic activity in vitro, and UPP-65 had the better effect.
- Undaria pinnatifida,
- polysaccharide,
- graded alcohol,
- basic components,
- hypoglycemic activity

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References

[1]	FENG S M, LUAN D, NING K, et al. Ultrafiltration isolation, hypoglycemic activity analysis and structural characterization of polysaccharides from Brasenia schreberi[J]. International Journal of Biological Macromolecules,2019,135:141−151. doi: 10.1016/j.ijbiomac.2019.05.129
[2]	WANG L, PARK Y J, JEON Y J, et al. Bioactivities of the edible brown seaweed, Undaria pinnatifida:A review[J]. Aquaculture,2018,495:873−880. doi: 10.1016/j.aquaculture.2018.06.079
[3]	ZHONG Q W, ZHOU T S, QIU W H, et al. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida[J]. Food Chemistry,2021,341(1):128−148.
[4]	MAK W, HAMID N, LIU T, et al. Fucoidan from New Zealand Undaria pinnatifida:Monthly variations and determination of antioxidant activities[J]. Carbohydrate Polymers,2013,95(1):606−614. doi: 10.1016/j.carbpol.2013.02.047
[5]	HAN Y, WU J, LIU T T, et al. Separation, characterization and anticancer activities of a sulfated polysaccharide from Undaria pinnatifida[J]. International Journal of Biological Macromolecules,2016,83:42−49. doi: 10.1016/j.ijbiomac.2015.11.049
[6]	LI Q, WANG X Y, WAN Y S, et al. In vivo immunomodulatory activity of fucoidan from brown alga Undaria pinnatifida in sarcoma 180-bearing mice[J]. Journal of Functional Foods,2023,103:105486. doi: 10.1016/j.jff.2023.105486
[7]	RAUS R A, NAWAWI W M F W, NASARUDDIN R R. Alginate and alginate composites for biomedical applications[J]. Asian J Pharm ScienceDirect,2021,16(3):280−306. doi: 10.1016/j.ajps.2020.10.001
[8]	FERNANDO I P S, KIM D, NAH J W, et al. Advances in functionalizing fucoidans and alginates (bio)polymers by structural modifications:A review[J]. Chemical Engineering Journal,2019,355:33−48. doi: 10.1016/j.cej.2018.08.115
[9]	邱文辉. 裙带菜中岩藻多糖的制备工艺及其降血糖活性研究[D]. 杭州: 浙江工业大学, 2021. [QIU W H. Study on the preparation process and hypoglycemic activity of fucoidan from Undaria pinnatifida[D]. Hangzhou: Zhengjiang University of Technology, 2021.] QIU W H. Study on the preparation process and hypoglycemic activity of fucoidan from Undaria pinnatifida[D]. Hangzhou: Zhengjiang University of Technology, 2021.
[10]	WANG Q, ZHENG Y F, ZHUANG W J, et al. Genome-wide transcriptional changes in type 2 diabetic mice supplemented with lotus seed resistant starch[J]. Food Chemistry,2018,264:427−434. doi: 10.1016/j.foodchem.2018.05.056
[11]	WU G J, LIU D, WAN Y J, et al. Comparison of hypoglycemic effects of polysaccharides from four legume species[J]. Food Hydrocolloids,2019,90:299−304. doi: 10.1016/j.foodhyd.2018.12.035
[12]	WANG J, JIN W H, ZHANG W J, et al. Hypoglycemic property of acidic polysaccharide extracted from Saccharina japonica and its potential mechanism[J]. Carbohydr Polymers,2013,95(1):143−147. doi: 10.1016/j.carbpol.2013.02.076
[13]	ZHENG Q, JIA R B, OU Z R, et al. Comparative study on the structural characterization and alpha-glucosidase inhibitory activity of polysaccharide fractions extracted from Sargassum fusiforme at different pH conditions[J]. International Journal of Biological Macromolecules,2022,194:602−610. doi: 10.1016/j.ijbiomac.2021.11.103
[14]	张涵, 殷澳, 张会佳, 等. 昆布多糖的复合酶法提取工艺优化及其对 α-葡萄糖苷酶的抑制活性[J]. 食品工业科技,2024,45(3):197−206. [ZHANG H, YIN A, ZHANG H J, et al. Optimization of the extraction technology of Laminaria polysaccharides by complex enzyme method and its inhibitory activity against α-glucosidase[J]. Science and Technology of Food Industry,2024,45(3):197−206.] ZHANG H, YIN A, ZHANG H J, et al. Optimization of the extraction technology of Laminaria polysaccharides by complex enzyme method and its inhibitory activity against α-glucosidase[J]. Science and Technology of Food Industry, 2024, 45（3）: 197−206.
[15]	张梦晴. 羊栖菜α-葡萄糖苷酶抑制剂的分离纯化及特性研究[D]. 无锡: 江南大学, 2020. [ZHANG M Q. Isolation, purification and characterization of α-glucosidase inhibitors from sheepsy cabbage[D]. Wuxi: Jiangnan University, 2020.] ZHANG M Q. Isolation, purification and characterization of α-glucosidase inhibitors from sheepsy cabbage[D]. Wuxi: Jiangnan University, 2020.
[16]	JIANG Z D, YU G, LIANG Y, et al. Inhibitory effects of a sulfated polysaccharide isolated from edible red alga Bangia fusco-purpurea on alpha-amylase and alpha-glucosidase[J]. Bioscience Biotechnology Biochemistry,2019,83(11):2065−2074. doi: 10.1080/09168451.2019.1634515
[17]	杜涓, 安晓萍, 刘娜, 等. 分级醇沉发酵麸皮多糖的成分分析与抗氧化活性研究[J]. 食品工业科技,2020,41(5):58−62. [DU J, AN X P, LIU N, et al. Composition analysis and antioxidant activity of bran polysaccharide in fractional alcohol precipitation fermentation[J]. Science and Technology of Food Industry,2020,41(5):58−62.] DU J, AN X P, LIU N, et al. Composition analysis and antioxidant activity of bran polysaccharide in fractional alcohol precipitation fermentation[J]. Science and Technology of Food Industry, 2020, 41（5）: 58−62.
[18]	景永帅, 金姗, 张丹参, 等. 北沙参乙醇分级多糖的理化性质及抗氧化活性研究[J]. 食品与机械,2020,36(7):175−180,226. [JING Y S, JIN S, ZHANG D S, et al. Study on physicochemical properties and antioxidant activity of ethanol graded polysaccharide of Radix Adenophora[J]. Food and Machinery,2020,36(7):175−180,226.] JING Y S, JIN S, ZHANG D S, et al. Study on physicochemical properties and antioxidant activity of ethanol graded polysaccharide of Radix Adenophora[J]. Food and Machinery, 2020, 36（7）: 175−180,226.
[19]	温文娟, 刘珊, 黄远丽. 苯酚硫酸法与蒽酮硫酸法测定香菇多糖含量比较[J]. 现代食品,2020,7(21):177−179. [WEN W J, LIU S, HUANG Y L, et al. Comparison of phenol sulfuric acid method and anthrone sulfuric acid method for determination of lentinan content[J]. Modern Food,2020,7(21):177−179.] WEN W J, LIU S, HUANG Y L, et al. Comparison of phenol sulfuric acid method and anthrone sulfuric acid method for determination of lentinan content[J]. Modern Food, 2020, 7（21）: 177−179.
[20]	冯东辉. 两种蛋白质测定方法比较研究[J]. 农业技术与装备,2019,34(8):16−17. [FENG D H. Comparative study of two methods for protein determination[J]. Agricultural Technology and Equipment,2019,34(8):16−17.] FENG D H. Comparative study of two methods for protein determination[J]. Agricultural Technology and Equipment, 2019, 34（8）: 16−17.
[21]	任珍芸, 刘爱萍, 陈晓航, 等. 改良间羟基联苯法用于测定肺炎球菌荚膜多糖中糖醛酸含量[J]. 中国新药杂志,2018,27(6):644−649. [REN Z Y, LIU A P, CHEN X H, et al. The modified M-hydroxybiphenyl method was used to determine the content of uronic acid in pneumococcal capsular polysaccharide[J]. Chinese Journal of New Drugs,2018,27(6):644−649.] REN Z Y, LIU A P, CHEN X H, et al. The modified M-hydroxybiphenyl method was used to determine the content of uronic acid in pneumococcal capsular polysaccharide[J]. Chinese Journal of New Drugs, 2018, 27（6）: 644−649.
[22]	杨丽红. 裙带菜多糖的分离纯化及抗肿瘤、免疫调节活性研究[D]. 广州: 广州中医药大学, 2020. [YANG L H. Isolation and purification of wakame polysaccharide and its antitumor and immunomodulatory activities[D]. Guangzhou: University of Chinese Medicine, 2020.] YANG L H. Isolation and purification of wakame polysaccharide and its antitumor and immunomodulatory activities[D]. Guangzhou: University of Chinese Medicine, 2020.
[23]	CHENG J W, SONG J L, WEI H L, et al. Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang Mycelia[J]. International Journal of Biological Macromolecules,2020,164:3305−3314. doi: 10.1016/j.ijbiomac.2020.08.202
[24]	YANG X, CAO D L, JI H Y, et al. Physicochemical characterization, rheological properties, and hypolipidemic and antioxidant activities of compound polysaccharides in Chinese herbal medicines by fractional precipitation[J]. International Journal of Biological Macromolecules,2023,242(1):124838.
[25]	陈冰洁, 乔勇进, 王晓, 等. 金瓜多糖不同分级组分的抗氧化和降血糖活性[J]. 食品与机械,2023,39(7):165−171. [CHEN B J, QIAO Y J, WANG X, et al. Antioxidant and hypoglycemic activities of different fractions of polysaccharide from cucumber[J]. Food and Machinery,2023,39(7):165−171.] CHEN B J, QIAO Y J, WANG X, et al. Antioxidant and hypoglycemic activities of different fractions of polysaccharide from cucumber[J]. Food and Machinery, 2023, 39（7）: 165−171.
[26]	张心驰, 惠和平, 郭栋费, 等. 梯度醇沉兰州百合多糖结构及降血糖活性分析[J]. 精细化工,2023,40(10):2231−2240. [ZHANG X C, HUI H P, GUO D F, et al. Analysis of structure and hypoglycemic activity of polysaccharide from Lanzhou Lilium by gradient alcohol precipitation[J]. Fine Chemicals,2023,40(10):2231−2240.] ZHANG X C, HUI H P, GUO D F, et al. Analysis of structure and hypoglycemic activity of polysaccharide from Lanzhou Lilium by gradient alcohol precipitation[J]. Fine Chemicals, 2023, 40（10）: 2231−2240.
[27]	ZHU H, XU L, WANG J, et al. Rheological behaviors of ethanol-fractional polysaccharides from Dendrobium officinale in aqueous solution:Effects of concentration, temperature, pH, and metal ions[J]. Food Hydrocolloids, 2023, 137:108311.
[28]	GU J Y, ZHANG H H, ZHANG J X, et al. Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L.[J]. Carbohydrate Polymers,2020,229:115355. doi: 10.1016/j.carbpol.2019.115355
[29]	杨鸿玲. 桑叶多糖的分离纯化及其组成研究[D]. 天津: 天津大学, 2007. [YANG H L. Isolation, purification and composition of polysaccharides from mulberry leaves[D]. Tianjin:Tianjin University, 2007.] YANG H L. Isolation, purification and composition of polysaccharides from mulberry leaves[D]. Tianjin: Tianjin University, 2007.
[30]	HENTATI F, DELATTRE C, URSU A V, et al. Structural characterization and antioxidant activity of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa[J]. Carbohydrate Polymers,2018,198:589−600. doi: 10.1016/j.carbpol.2018.06.098
[31]	PENG X B, LI Q, OU L N, et al. GC-MS, FT-IR analysis of black fungus polysaccharides and its inhibition against skin aging in mice[J]. International Journal of Biological Macromolecules,2010,47(2):304−307. doi: 10.1016/j.ijbiomac.2010.03.018
[32]	BARROS F C, DA SILVA D C, SOMBRA V G, et al. Structural characterization of polysaccharide obtained from red seaweed Gracilaria caudata (J Agardh)[J]. Carbohydrate Polymers,2013,92(1):598−603. doi: 10.1016/j.carbpol.2012.09.009
[33]	LI R, CHEN W C, WANG W P, et al. Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA[J]. Carbohydrate Polymers,2010,82(2):240−244. doi: 10.1016/j.carbpol.2010.02.048
[34]	CHEN Z Y, ZHAO Y, ZHANG M K, et al. Structural characterization and antioxidant activity of a new polysaccharide from Bletilla striata fibrous roots[J]. Carbohydrate Polymers,2020,227:1153−1162.
[35]	TAN M H, ZHAO Q S, ZHAO B. Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds[J]. International Journal of Biological Macromolecules, 2021, 193(2):1635-1644.
[36]	LI Q, WANG W, ZHU Y, et al. Structural elucidation and antioxidant activity a novel Se-polysaccharide from Se-enriched Grifola frondosa[J]. Carbohydrate Polymers,2017,161:42−52. doi: 10.1016/j.carbpol.2016.12.041
[37]	陈舒桐, 周庆玲, 杨睿宇, 等. 两种马尾藻岩藻多糖的理化性质、结构表征及其增强免疫和降血糖活性[J]. 食品科学,2024,45(1):15−22. [CHEN S T, ZHOU Q L, YANG R Y, et al. Physicochemical properties, structural characterization and immune-enhancing and hypoglycemic activities of two kinds of Sargassum fucoidan polysaccharides[J]. Food Science,2024,45(1):15−22.] CHEN S T, ZHOU Q L, YANG R Y, et al. Physicochemical properties, structural characterization and immune-enhancing and hypoglycemic activities of two kinds of Sargassum fucoidan polysaccharides[J]. Food Science, 2024, 45（1）: 15−22.
[38]	刘贵阁, 钟耀广, 陈冰洁, 等. 不同醇沉米糠多糖的体外抗氧化和降血糖活性研究[J]. 保鲜与加工,2023,23(3):29−36. [LIU G G, ZHONG Y G, CHEN B J, et al. Study on antioxidant and hypoglycemic activities of rice bran polysaccharides with different alcohols in vitro[J]. Storage and Process,2023,23(3):29−36.] LIU G G, ZHONG Y G, CHEN B J, et al. Study on antioxidant and hypoglycemic activities of rice bran polysaccharides with different alcohols in vitro[J]. Storage and Process, 2023, 23（3）: 29−36.
[39]	XU Y Q, NIU X J, LIU N Y, et al. Characterization, antioxidant and hypoglycemic activities of degraded polysaccharides from blackcurrant ( Ribes nigrum L.) fruits[J]. Food Chemistry,2018,243:26−35. doi: 10.1016/j.foodchem.2017.09.107
[40]	CHEN S H, CHEN H X, TIAN J G, et al. Chemical modification, antioxidant and alpha-amylase inhibitory activities of corn silk polysaccharides[J]. Carbohydrate Polymers,2013,98(1):428−437. doi: 10.1016/j.carbpol.2013.06.011
[41]	WANG B H, CAO J J, ZHANG B, et al. Structural characterization, physicochemical properties and alpha-glucosidase inhibitory activity of polysaccharide from the fruits of wax apple[J]. Carbohydrate Polymers,2019,211:227−236. doi: 10.1016/j.carbpol.2019.02.006

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