Preparation and Structural Characterization of Ferrous Chelates of <i>Spirulina platensis</i> Peptides

ZHANG Yu; LI Wei; WANG Lijuan; LI Fangwei; ZENG Mingyong

doi:10.13386/j.issn1002-0306.2023060103

Volume 45 Issue 10

May 2024

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Science and Technology of Food Industry > 2024 > 45(10): 165-175. > DOI: 10.13386/j.issn1002-0306.2023060103

ZHANG Yu, LI Wei, WANG Lijuan, et al. Preparation and Structural Characterization of Ferrous Chelates of Spirulina platensis Peptides[J]. Science and Technology of Food Industry, 2024, 45(10): 165−175. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060103.

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Preparation and Structural Characterization of Ferrous Chelates of Spirulina platensis Peptides

ZHANG Yu^{1, 2,},
LI Wei^{1, 2},
WANG Lijuan^{1, 2},
LI Fangwei^{1, 2, ,},
ZENG Mingyong^{1, 2, ,}

1.
Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China
2.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

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Received Date: June 11, 2023
Available Online: March 21, 2024

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Abstract

Abstract

In order to improve the high-value utilization of Spirulina platensis, the chelating conditions of Spirulina platensis protein peptide were preliminatively optimized with ferrous chloride as the iron source. The chelates were characterized by molecular weight determination, amino acid composition experiment, fluorescence spectrum, scanning electron microscopy and quantum chemical calculation. The results showed that the suitable chelating conditions were pH6, mass ratio of ferrous peptide 6:1, concentration of peptide solution 1%, temperature 60 ℃ and time 40 min. Under these conditions, the chelating yield was 44.13%. The peptide of Spirulina platensis produced a new structure after chelating with ferric salt. Iron was mainly combined with the carboxyl group of glutamic acid and aspartate, the amino group of arginine and the guanidine group of lysine, and was embedded in the cavity formed by the electronegative end of amino acid to form a stable structure, which proved the formation of chelates. The research results would provide a theoretical basis for the development of Spirulina platensis iron supplement.

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References

[1]	LAFARGA T, ACIÉN-FERNÁNDEZ F G, GARCIA-VAQUERO M. Bioactive peptides and carbohydrates from seaweed for food applications:Natural occurrence, isolation, purification, and identification[J]. Algal Research,2020,48:101909. doi: 10.1016/j.algal.2020.101909
[2]	LI B, HE H, SHI W, et al. Effect of duck egg white peptide-ferrous chelate on iron bioavailability in vivo and structure characterization[J]. Journal of the Science of Food and Agriculture,2019,99(4):1834−1841. doi: 10.1002/jsfa.9377
[3]	AI X, YU P, LI X, et al. Polysaccharides from Spirulina platensis:Extraction methods, structural features and bioactivities diversity[J]. International Journal of Biological Macromolecules, 2023:123211.
[4]	ZIMMERMANN M B, HURRELL R F. Nutritional iron deficiency[J]. The Lancet,2007,370(9586):511−520. doi: 10.1016/S0140-6736(07)61235-5
[5]	CIAN R E, GARZÓN A G, ANCONA D B, et al. Chelating properties of peptides from red seaweed Pyropia columbina and its effect on iron bio-accessibility[J]. Plant Foods for Human Nutrition,2016,71:96−101. doi: 10.1007/s11130-016-0533-x
[6]	FAN C, WANG X, SONG X, et al. Identification of a novel walnut iron chelating peptide with potential high antioxidant activity and analysis of its possible binding sites[J]. Foods,2023,12(1):226. doi: 10.3390/foods12010226
[7]	HE Yuanqing, YANG Pengyao, DING Yangyang, et al. The preparation, antioxidant activity evaluation, and iron-deficient anemic improvement of oat (Avena sativa L.) peptides–ferrous chelate[J]. Frontiers in Nutrition,2021,8:687133. doi: 10.3389/fnut.2021.687133
[8]	LÜ Y, GUO S, TAKO E, et al. Hydrolysis of soybean protein improves iron bioavailability by Caco-2 cell[J]. Journal of Food and Nutrition Research,2014,2(4):162−166. doi: 10.12691/jfnr-2-4-5
[9]	HUANG G R, REN Z Y, JIANG J X. Optimization of hydrolysis conditions for iron binding peptides production from shrimp processing byproducts[J]. American Journal of Food Technology,2014,9(1):49−55.
[10]	LIN S, HU X, LI L, et al. Preparation, purification and identification of iron-chelating peptides derived from tilapia (Oreoch romis niloticus) skin collagen and characterization of the peptide-iron complexes[J]. LWT,2021,149:111796. doi: 10.1016/j.lwt.2021.111796
[11]	QU W, FENG Y, XIONG T, et al. Preparation of corn ACE inhibitory peptide-ferrous chelate by dual-frequency ultrasound and its structure and stability analyses[J]. Ultrasonics Sonochemistry,2022,83:105937. doi: 10.1016/j.ultsonch.2022.105937
[12]	FAN C, GE X, HAO J, et al. Identification of high iron–chelating peptides with unusual antioxidant effect from sea cucumbers and the possible binding mode[J]. Food Chemistry,2023,399:133912. doi: 10.1016/j.foodchem.2022.133912
[13]	孙宜君. 螺旋藻抗菌肽的纯化鉴定及其抑菌机理的研究[D]. 北京:北京林业大学, 2016. [SUN Yijun. Purification, identification and antibacterial mechanism of Spirulina antibacterial peptides[D]. Beijing:Beijing Forestry University, 2016.] SUN Yijun. Purification, identification and antibacterial mechanism of Spirulina antibacterial peptides[D]. Beijing: Beijing Forestry University, 2016.
[14]	庞忠莉. 牡蛎肽亚铁螯合物的制备及性质研究[D]. 广州:华南理工大学, 2020. [PANG Zhongli. Preparation and properties of ferrous peptide chelate from oyster[D]. Guangzhou:South China University of Technology, 2020.] PANG Zhongli. Preparation and properties of ferrous peptide chelate from oyster[D]. Guangzhou: South China University of Technology, 2020.
[15]	廉雯蕾. 脱酰胺—酶解法制备米蛋白肽及其亚铁螯合物的研究[D]. 无锡:江南大学, 2015. [LIAN Wenlei. Preparation of rice protein peptides and their ferrous chelates by deamide-enzymatic hydrolysis[D]. Wuxi:Jiangnan University, 2015.] LIAN Wenlei. Preparation of rice protein peptides and their ferrous chelates by deamide-enzymatic hydrolysis[D]. Wuxi: Jiangnan University, 2015.
[16]	LI F, CAO J, WANG Z, et al. Dual aggregation in ground state and ground-excited state induced by high concentrations contributes to chlorophyll stability[J]. Food Chemistry,2022,383:132447. doi: 10.1016/j.foodchem.2022.132447
[17]	LU T, CHEN F. Multiwfn:A multifunctional wavefunction analyzer[J]. Journal of Computational Chemistry,2012,33(5):580−92. doi: 10.1002/jcc.22885
[18]	SUN N, CUI P, JIN Z, et al. Contributions of molecular size, charge distribution, and specific amino acids to the iron-binding capacity of sea cucumber (Stichopus japonicus) ovum hydrolysates[J]. Food Chemistry,2017,230:627−636. doi: 10.1016/j.foodchem.2017.03.077
[19]	ZHANG Y, DING X, LI M. Preparation, characterization and in vitro stability of iron-chelating peptides from mung beans[J]. Food Chemistry,2021,349:129101. doi: 10.1016/j.foodchem.2021.129101
[20]	ZHANG S Q, YU X F, ZHANG H B, et al. Comparison of the oral absorption, distribution, excretion, and bioavailability of zinc sulfate, zinc gluconate, and zinc-enriched yeast in rats[J]. Molecular Nutrition & Food Research,2018,62(7):1700981.
[21]	REDDI A R, GUZMAN T R, BREECE R M, et al. Deducing the energetic cost of protein folding in zinc finger proteins using designed metallopeptides[J]. Journal of the American Chemical Society,2007,129(42):12815−12827. doi: 10.1021/ja073902+
[22]	杨玉蓉. 西藏野桃仁酶解多肽的生物活性及其亚铁螯合物的研究[D]. 长沙:中南林业科技大学, 2019. [YANG Yurong. Study on the bioactivity of enzymolysis polypeptides and their ferrous chelates from Tibetan wild peach kernel[D]. Changsha:Central South University of Forestry and Technology, 2019.] YANG Yurong. Study on the bioactivity of enzymolysis polypeptides and their ferrous chelates from Tibetan wild peach kernel[D]. Changsha: Central South University of Forestry and Technology, 2019.
[23]	UPPAL R, LAKSHMI K V, VALENTINE A M. Isolation and characterization of the iron-binding properties of a primitive monolobal transferrin from Ciona intestinalis[J]. JBIC Journal of Biological Inorganic Chemistry,2008,13:873−885. doi: 10.1007/s00775-008-0375-6
[24]	CAETANO-SILVA M E, NETTO F M, BERTOLDO-PACHECO M T, et al. Peptide-metal complexes:Obtention and role in increasing bioavailability and decreasing the pro-oxidant effect of minerals[J]. Critical Reviews in Food Science and Nutrition,2021,61(9):1470−1489. doi: 10.1080/10408398.2020.1761770
[25]	CUI P, SUN N, JIANG P, et al. Optimised condition for preparing sea cucumber ovum hydrolysate–calcium complex and its structural analysis[J]. International Journal of Food Science & Technology,2017,52(8):1914−1922.
[26]	TIAN Q, FAN Y, HAO L, et al. A comprehensive review of calcium and ferrous ions chelating peptides:Preparation, structure and transport pathways[J]. Critical Reviews in Food Science and Nutrition, 2021:1−13.
[27]	CAO Y, ROMERO J, OLSON J P, et al. Quantum chemistry in the age of quantum computing[J]. Chemical Reviews,2019,119(19):10856−10915. doi: 10.1021/acs.chemrev.8b00803
[28]	SUN N, WANG T, WANG D, et al. Antarctic krill derived nonapeptide as an effective iron-binding ligand for facilitating iron absorption via the small intestine[J]. Journal of Agricultural and Food Chemistry,2020,68(40):11290−11300. doi: 10.1021/acs.jafc.0c03223
[29]	RAFE A, RAZAVI S M A. Effect of thermal treatment on chemical structure of β-lactoglobulin and basil seed gum mixture at different states by ATR-FTIR spectroscopy[J]. International Journal of Food Properties,2015,18(12):2652−2664. doi: 10.1080/10942912.2014.999864
[30]	ZHOU J, WANG X, AI T, et al. Preparation and characterization of β-lactoglobulin hydrolysate-iron complexes[J]. Journal of Dairy Science,2012,95(8):4230−4236. doi: 10.3168/jds.2011-5282
[31]	CHEN M, JI H, ZHANG Z, et al. A novel calcium-chelating peptide purified from Auxis thazard protien hydrolysate and its binding properties with calcium[J]. Journal of Functional Foods,2019,60:103447. doi: 10.1016/j.jff.2019.103447
[32]	孙如男. 南极磷虾肽-锌螯合物的制备, 结构表征与生物利用度研究[D]. 青岛:青岛大学, 2021. [SUN Runan. Preparation, characterization and bioavailability of peptide-Zinc chelates from Krill Antarctic[D]. Qingdao:Qingdao University, 2021.] SUN Runan. Preparation, characterization and bioavailability of peptide-Zinc chelates from Krill Antarctic[D]. Qingdao: Qingdao University, 2021.
[33]	LIU F R, WANG L, WANG R, et al. Calcium-binding capacity of wheat germ protein hydrolysate and characterization of peptide–calcium complex[J]. Journal of Agricultural and Food Chemistry,2013,61(31):7537−7544. doi: 10.1021/jf401868z

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