Screening Quality Markers of Regulating Lipid Metabolism Activity of <i>Rubus suavissimus</i> S. Lee Based on Spectral Effect Relationship

FAN Lanlan; WU Qiulian; HUANG Wanfang; JIANG Manjing; PAN Dongjin; LING Jie

doi:10.13386/j.issn1002-0306.2023060025

Volume 45 Issue 13

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(13): 17-29. > DOI: 10.13386/j.issn1002-0306.2023060025

FAN Lanlan, WU Qiulian, HUANG Wanfang, et al. Screening Quality Markers of Regulating Lipid Metabolism Activity of Rubus suavissimus S. Lee Based on Spectral Effect Relationship[J]. Science and Technology of Food Industry, 2024, 45(13): 17−29. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060025.

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Screening Quality Markers of Regulating Lipid Metabolism Activity of Rubus suavissimus S. Lee Based on Spectral Effect Relationship

1.
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
2.
Key Laboratory of Extraction Purification and Quality Analysis, Nanning 530200, China
3.
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China

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Received Date: June 05, 2023
Available Online: May 06, 2024

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Abstract

Abstract

Objective: The study was designed to test solvent extracts of Rubus suavissimus (RS) for compounds that regulate lipid metabolism and to measure their relative activity as quantitative markers of lipid metabolism-regulating activities based on the spectrum-effect relationship. Method: RS was extracted with 95% ethanol, the different extraction parts were successively extracted with petroleum ether, methylene chloride, ethyl acetate and n-butanol, respectively. The solution of different extraction parts was applied to the induced differentiated 3T3-L1 preadipocytes, and the oil red O staining and triglyceride release from the cells were determined to screen the best metabolism-regulating site. Analysis of different extraction sited by UPLC-Q-TOF-MS/MS technique to separate and identify the components. Based on specific assays, image intensity analysis, grey correlation and partial least squares regressions, the spectrum-effect relationship of the most abundant active components and their ability to regulate lipid metabolism were determined, and the quality markers were screened. Results: Ethyl acetate and n-butanol extraction site was the best metabolism-regulating site. Four components, including ellagic acid, centaurin-3-O-rutinoside, rubusoside and enantio-kauri-16-ene-19-carboxylic acid-13-O-β-D-glucoside were highly associated with metabolism-regulating effects. It could be used as quality markers for regulating lipid metabolism. Conclusion: Investigation of the quality markers of RS extracts based on the spectrum-effect relationship is of great importance for elucidating the pharmacodynamics, screening the core quality markers for therapeutic activity, and ensuring the safety and rational application of traditional medicines.
- Rubus suavissimus,
- 3T3-L1 cells,
- lipid metabolism,
- spectrum-effect relationship,
- quality marker

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