Analysis of the Components of <i>Cimicifuga dahurica</i> (Turcz.) Maxim. Alcohol Extract and Exploration of Its Mechanism of Promoting Skin Wound Healing Based on Network Pharmacology

LI Yanna; ZHANG Fuyuan; CHENG Weifeng; SANG Yaxin; WANG Xianghong

doi:10.13386/j.issn1002-0306.2023050247

Volume 44 Issue 24

Dec. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(24): 12-22. > DOI: 10.13386/j.issn1002-0306.2023050247

LI Yanna, ZHANG Fuyuan, CHENG Weifeng, et al. Analysis of the Components of Cimicifuga dahurica (Turcz.) Maxim. Alcohol Extract and Exploration of Its Mechanism of Promoting Skin Wound Healing Based on Network Pharmacology[J]. Science and Technology of Food Industry, 2023, 44(24): 12−22. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050247.

Citation:

PDF (13987 KB)

Analysis of the Components of Cimicifuga dahurica (Turcz.) Maxim. Alcohol Extract and Exploration of Its Mechanism of Promoting Skin Wound Healing Based on Network Pharmacology

1.
College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China
2.
Hebei Yaguo Food Co., Ltd., Baoding 071000, China

More Information

Received Date: May 21, 2023
Available Online: October 20, 2023

Graphical Abstract

Abstract

Abstract

In order to further explore the physiological functions of Cimicifuga dahurica (Turcz.) Maxim., the study extracted the active compounds by alcohol extraction method, analyzed the chemical compositions by HPLC-MS, and explored its healing mechanism on skin wounds based on network pharmacology. The results showed that 125 chemical components were identified from Cimicifuga dahurica (Turcz.) Maxim., and the contents of terpenoids, phenylpropanoids and ketones were higher, including cryptochlorogenic acid, chlorogenic acid, atractylenolide III and others. The active compounds of Cimicifuga dahurica (Turcz.) Maxim. could regulate one or more targets. The EGFR, KDR, F2 genes might be important targets in the process of promoting healing, and tangeretin, alpinetin, and 5-O-demethylnobiletin and other active compounds might also play important roles in this process. It was proved that Cimicifuga dahurica (Turcz.) Maxim. had a characteristic of multi-component and multi-target action to promote skin wound healing. After the action of Cimicifuga dahurica (Turcz.) Maxim., the expression of related factors and proteins might be regulated through VEGF, EGFR, and TNF signaling pathways, exerting a promoting effect on skin wound healing. Through the above research, the main active ingredients of Cimicifuga dahurica (Turcz.) Maxim. alcohol extract were identified, and the mechanism of promoting skin wound healing was predicted based on network pharmacology, which would provide the experimental and theoretical basis for the subsequent development and utilization of Cimicifuga dahurica (Turcz.) Maxim..
- Cimicifuga dahurica (Turcz.) Maxim.,
- compound composition,
- promote skin wound healing,
- network pharmacology,
- mechanism

FullText(HTML)

References (36)

References

[1]	LI J X, YU Z Y. Cimicifugae rhizoma:From origins, bioactive constituents to clinical outcomes[J]. Current Medicinal Chemistry,2006,13(24):2927−2951. doi: 10.2174/092986706778521869
[2]	中华人民共和国药典[S]. 一部, 2020. [Pharmacopoeia of the People's Republic of China[S]. Part A, 2020.] Pharmacopoeia of the People's Republic of China[S]. Part A, 2020.
[3]	裴秋燕. 兴安升麻根茎的化学成分研究[D]. 成都:西南交通大学, 2012. [PEI Q Y. Chemical constituents from the roots of Cimicifuga dahurica (Turcz.) Maxim.[D]. Chengdu:Southwest Jiaotong University, 2012.] PEI Q Y. Chemical constituents from the roots of Cimicifuga dahurica (Turcz.) Maxim.[D]. Chengdu: Southwest Jiaotong University, 2012.
[4]	韩丽丽, 翟国鹏. 保护地升麻栽培与食用价值[J]. 中国农业信息,2014,17(26):37. [HAN L L, ZHAI G P. Protective Cimicifuga foetida L. cultivation and edible value[J]. China Agriculture Information,2014,17(26):37.] HAN L L, ZHAI G P. Protective Cimicifuga foetida L. cultivation and edible value[J]. China Agriculture Information, 2014, 17（26）: 37.
[5]	吕弘, 王晓明, 刘小梅, 等. 基于超高效液相色谱-四极杆-飞行时间质谱法的兴安升麻甲醇提取物化学成分分析[J]. 食品科学,2019,40(16):169−176. [LÜ H, WANG X M, LIU X M, et al. Identification of chemical constituents of methanol extract of Cimicifuga dahurica by ultra performance liquid chromatography coupled with quadrupole-time of flight-tandem mass spectrometry[J]. Food Science,2019,40(16):169−176.] LÜ H, WANG X M, LIU X M, et al. Identification of chemical constituents of methanol extract of Cimicifuga dahurica by ultra performance liquid chromatography coupled with quadrupole-time of flight-tandem mass spectrometry[J]. Food Science, 2019, 40（16）: 169−176.
[6]	王宏霞, 王秀丽. 兴安升麻研究进展[J]. 教育教学论坛,2019,439(45):111−112. [WANG H X, WANG X L. Research progress of Cimicifuga dahurica (Turcz.) Maxim.[J]. Education Teaching Forum,2019,439(45):111−112.] WANG H X, WANG X L. Research progress of Cimicifuga dahurica (Turcz.) Maxim.[J]. Education Teaching Forum, 2019, 439（45）: 111−112.
[7]	YAN J J, JUNG J S, KIM T K, et al. Protective effects of ferulic acid in amyloid precursor protein plus presenilin-1 transgenic mouse model of Alzheimer disease[J]. Biological and Pharmaceutical Bulletin,2013,36(1):140−143. doi: 10.1248/bpb.b12-00798
[8]	NIAN Y, WANG H Y, ZHOU L, et al. Cytotoxic cycloartane triterpenes of the traditional Chinese medicine―"shengma" (Cimicifuga dahurica)[J]. Planta Medica,2013,79(1):60−69.
[9]	QIN R L, ZHAO Y, ZHZO Y D, et al. Polyphenolic compounds with antioxidant potential and neuro-protective effect from Cimicifuga dahurica (Turcz.) Maxim.[J]. Fitoterapia,2016,115:52−56. doi: 10.1016/j.fitote.2016.09.016
[10]	LV C N, YANG F, QIN R L, et al. Bioactivity-guided isolation of chemical constituents against H₂O₂-induced neurotoxicity on PC12 from Cimicifuga dahurica (Turcz.) Maxim.[J]. Bioorganic & Medicinal Chemistry Letters,2017,27(15):3305−3309.
[11]	李龙剑. 芦荟苷氧化石墨烯凝胶的制备及其对创伤修复作用的研究[D]. 杭州:浙江大学, 2017. [LI L J. Study on the preparation of aloin-loaded graphene oxide gel and its effect on wound healing[D]. Hangzhou:Zhejiang University, 2017.] LI L J. Study on the preparation of aloin-loaded graphene oxide gel and its effect on wound healing[D]. Hangzhou: Zhejiang University, 2017.
[12]	刘文曾, 李峰. 影响伤口愈合的因素[J]. 中华现代外科学杂志, 2006:2186−2187. [LIU W Z, LI F. Factors affecting wound healing[J]. China Journal of Modern Medicine, 2006:2186−2187.] LIU W Z, LI F. Factors affecting wound healing[J]. China Journal of Modern Medicine, 2006: 2186−2187.
[13]	FAYNE R A, BORDA L J, EGGER A N, et al. The potential impact of social genomics on wound healing[J]. Advances in Wound Care,2020,9(6):325−331. doi: 10.1089/wound.2019.1095
[14]	LI Y N, SANG Y X, YU W L, et al. Antibacterial actions of Ag nanoparticles synthesized from Cimicifuga dahurica (Turcz.) Maxim. and their application in constructing a hydrogel spray for healing skin wounds[J]. Food Chemistry,2023,418:135981. doi: 10.1016/j.foodchem.2023.135981
[15]	DENG Y L, YE X W, CHEN Y F, et al. Chemical characteristics of platycodon grandiflorum and its mechanism in lung cancer treatment[J]. Frontiers in Pharmacology,2020,11:609825.
[16]	LUO T T, LU Y, YAN S K, et al. Network pharmacology in research of Chinese medicine formula:Methodology, application and prospective[J]. Chinese Journal of Integrative Medicine,2020,26(1):74−82.
[17]	袁家威, 张海涛, 揭珂, 等. 基于网络药理学研究桃红四物汤治疗假体周围感染的潜在靶点和机制[J]. 中国组织工程研究,2021,25(9):1428−1433. [YUAN J W, ZHANG H T, JIE K, et al. Underlying targets and mechanism of Taohong Siwu decoction in prosthetic joint infection on network pharmacology[J]. Chinese Journal of Tissue Engineering Research,2021,25(9):1428−1433.] doi: 10.3969/j.issn.2095-4344.4012 YUAN J W, ZHANG H T, JIE K, et al. Underlying targets and mechanism of Taohong Siwu decoction in prosthetic joint infection on network pharmacology[J]. Chinese Journal of Tissue Engineering Research, 2021, 25（9）: 1428−1433. doi: 10.3969/j.issn.2095-4344.4012
[18]	刘学楠. 苦豆子不同提取物化学成分分析及其肝毒性研究[D]. 兰州:甘肃农业大学, 2021. [LIU X N. The analysis of chemical constituents and the study of hepatotoxicity of different extracts of Sophora alopecuroides L D]. Lanzhou:Gansu Agricultural University, 2021.
[19]	IWANAGA A, KUSANO G, WARASHINA T, et al. Hyaluronidase inhibitors from "Cimicifugae Rhizoma" (a mixture of the rhizomes of Cimicifuga dahurica and C. heracleifolia)[J]. Journal of Natural Products,2010,73(4):573−578. doi: 10.1021/np900675n
[20]	ZHOU C X, YU Y E, SHENG R, et al. Cimicifoetones A and B, dimeric prenylindole alkaloids as black pigments of Cimicifuga foetida[J]. Chemistry-An Asian Journal,2017,12(12):1277−1281. doi: 10.1002/asia.201700348
[21]	葛玉琰. 兴安升麻抗病毒活性成分及其质量标准研究[D]. 哈尔滨:哈尔滨商业大学, 2017. [GE Y Y. Study on antiviral activity and quality standard of Cimicifuga dahuiica[D]. Harbin:Harbin University of Comerce, 2017.] GE Y Y. Study on antiviral activity and quality standard of Cimicifuga dahuiica[D]. Harbin: Harbin University of Comerce, 2017.
[22]	孙启泉, 左爱侠, 张婷婷. 升麻属植物化学成分、生物活性及临床应用研究进展[J]. 中草药,2017,48(14):3005−3016. [SUN Q Q, ZUO A X, ZHANG T T. Advances in studies on chemical constituents in plants of Cimicifuga L. and their bioactivities and clinical application[J]. Chinese Traditional and Herbal Drugs,2017,48(14):3005−3016.] SUN Q Q, ZUO A X, ZHANG T T. Advances in studies on chemical constituents in plants of Cimicifuga L. and their bioactivities and clinical application[J]. Chinese Traditional and Herbal Drugs, 2017, 48（14）: 3005−3016.
[23]	周瑞. 复方黄柏液及其成分黄连素抗糖尿病溃疡作用及分子机制研究[D]. 昆明:云南中医药大学, 2020. [ZHOU R. Study on anti-diabetic ulcer effect and molecular mechanism of Huangbai liniment and its component berberine[D]. Kunming:Yunnan University of Chinese Medicine, 2020.] ZHOU R. Study on anti-diabetic ulcer effect and molecular mechanism of Huangbai liniment and its component berberine[D]. Kunming: Yunnan University of Chinese Medicine, 2020.
[24]	张易, 顾瑜. 绿原酸的抑菌作用及其在口腔中的应用进展[J]. 医药论坛杂志,2022,43(7):104−107. [ZHANG Y, GU Y. Bacteriostasis of chlorogenic acid and its application in oral cavity[J]. Journal of Medical Forum,2022,43(7):104−107.] ZHANG Y, GU Y. Bacteriostasis of chlorogenic acid and its application in oral cavity[J]. Journal of Medical Forum, 2022, 43（7）: 104−107.
[25]	刘云宁, 李小凤, 班旭霞, 等. 中药抗菌成分及其抗菌机制的研究进展[J]. 环球中医药,2015,8(8):1012−1017. [LIU Y N, LI X F, BAN X X, et al. The review on active antibacterial ingredients of Chinese medicine and the antibacterial mechanism[J]. Global Traditional Chinese Medicine,2015,8(8):1012−1017.] LIU Y N, LI X F, BAN X X, et al. The review on active antibacterial ingredients of Chinese medicine and the antibacterial mechanism[J]. Global Traditional Chinese Medicine, 2015, 8（8）: 1012−1017.
[26]	LEE M H, HAN A R, JANG M, et al. Antiskin inflammatory activity of black ginger (Kaempferia parviflora) through antioxidative activity[J]. Oxidative Medicine and Cellular Longevity,2018,2018(5):5967150.
[27]	BAS E, RECIO M C, GINER R M, et al. Anti-inflammatory activity of 5-O-demethylnobiletin, a polymethoxyflavone isolated from Sideritis tragoriganum[J]. Planta Medica,2006,72(2):136−142. doi: 10.1055/s-2005-873191
[28]	谭悦. 山姜素对溃疡性结肠炎肠上皮屏障作用的影响[D]. 沈阳:中国医科大学, 2018. [TAN Y. Effects of alpinetin on intestinal epithelial barrier in ulcerative colitis[D]. Shenyang:China Medical University, 2018.] TAN Y. Effects of alpinetin on intestinal epithelial barrier in ulcerative colitis[D]. Shenyang: China Medical University, 2018.
[29]	LEMMON M A, SCHLESSINGER J, FERGUSON K M. The EGFR family:Not so prototypical receptor tyrosine kinases[J]. Cold Spring Harbor Perspectives in Biology,2014,6(4):a020768. doi: 10.1101/cshperspect.a020768
[30]	MEYER R D, RAHIMI N. Comparative structure-function analysis of VEGFR-1 and VEGFR-2:What have we learned from chimeric systems?[J]. Annals of the New York Academy of Sciences,2003,995:200−207. doi: 10.1111/j.1749-6632.2003.tb03223.x
[31]	AKHAVAN S, MANNUCCI P M, LAK M, et al. Identification and three-dimensional structural analysis of nine novel mutations in patients with prothrombin deficiency[J]. Journal of Thrombosis and Haemostasis: List of Issues,2000,84(6):989−997.
[32]	ELSHABRAWY H A, CHEN Z, VOLIN M V, et al. The pathogenic role of angiogenesis in rheumatoid arthritis[J]. Angiogenesis,2015,18(4):433−438. doi: 10.1007/s10456-015-9477-2
[33]	李全, 崔凤瑞, 巴特, 等. 脂肪干细胞来源的外泌体促进肉芽组织来源的成纤维细胞增殖的初步研究[J]. 中华损伤与修复杂志(电子版),2019,14(2):91−96. [LI Q, CUI F R, BA T, et al. Preliminary study of promotion effect of adipose derived stem cell-exosomes on fibroblast proliferation derived from granulation tissues[J]. Chinese Journal of Injury Repair and Wound Healing (Electronic Edition),2019,14(2):91−96.] LI Q, CUI F R, BA T, et al. Preliminary study of promotion effect of adipose derived stem cell-exosomes on fibroblast proliferation derived from granulation tissues[J]. Chinese Journal of Injury Repair and Wound Healing (Electronic Edition), 2019, 14（2）: 91−96.
[34]	RODRIGUES M, KOSARIC N, BONHAM C A, et al. Wound healing:A cellular perspective[J]. Physiological Reviews,2019,99(1):665−706. doi: 10.1152/physrev.00067.2017
[35]	来梦婕. 墨西哥黄唇鱼胶原肽促进小鼠皮肤伤口愈合作用的研究[D]. 杭州:浙江大学, 2018. [LAI M J. Effect of totoaba macdonaldi-ment collagen peptides on wound healing in mice[D]. Hangzhou:Zhejiang University, 2018.] LAI M J. Effect of totoaba macdonaldi-ment collagen peptides on wound healing in mice[D]. Hangzhou: Zhejiang University, 2018.
[36]	ENOCH S, LEAPER D J. Basic science of wound healing[J]. Surgery (Oxford),2008,26(2):31−37.

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	胡秀发，陈燕美，张喜玲，顾辰琦，李曼，宁阳阳，关纬超，郑婵敏，董茗洋，胡蝶，杨庆余. 超声辅助NaCl浸泡对熟化黑豆结构和消化特性的影响. 食品工业科技. 2024(06): 121-127 . 本站查看
2.	陈楚瑶，李丹丹，陶阳，张荣广，韩永斌. 微波预处理对红豆吸水特性、外观及淀粉特性的影响. 食品工业科技. 2023(01): 56-64 . 本站查看
3.	曲敏，贾圆芳，刘琳琳，朱秀清，朱颖，黄雨洋，孙冰玉，吕铭守. 不同复水方式对大豆拉丝蛋白复水品质的影响及动力学模型构建. 农业工程学报. 2023(10): 264-274 .
4.	李娜，线承源，徐鹏云，姜海勇，李姜维，王杉. 皂荚籽热风干燥特性及干燥动力学模型研究. 食品安全质量检测学报. 2023(19): 252-261 .