Simultaneous Determination of Benzimidazoles and Their Metabolites Residues in Dairy Products by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

QIAO Yongsheng; WANG Ping; HUANG Yinbo; FENG Yinjie; CAI Xia; ZHANG Zhanlin; CHEN Wei

doi:10.13386/j.issn1002-0306.2023050242

Volume 45 Issue 13

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(13): 221-229. > DOI: 10.13386/j.issn1002-0306.2023050242

QIAO Yongsheng, WANG Ping, HUANG Yinbo, et al. Simultaneous Determination of Benzimidazoles and Their Metabolites Residues in Dairy Products by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2024, 45(13): 221−229. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050242.

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Simultaneous Determination of Benzimidazoles and Their Metabolites Residues in Dairy Products by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Taizhou Institute for Food Control, Key Laboratory of FSMP Risk Identification and Key Analysis Technology for Jiangsu Market Regulation, Taizhou 225300, China

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Received Date: May 21, 2023
Available Online: May 06, 2024

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Abstract

Abstract

A method for the simultaneous determination of benzimidazoles and their metabolites in dairy products was established using ultra high performance liquid chromatography-tandem mass spectrometry (MS). After extracting the samples with acetonitrile containing 0.2% formic acid, the extracts were centrifuged and purified by solid phase extraction column, and then separated by UPLC HSS T₃ chromatographic column, determined in multi reaction monitoring (MRM) method with a positive ESI mode. The results showed that the linear correlation coefficient (r) within the range of 1.0~50 μg/L was greater than 0.995 (except for triclabendazole, which was 0.9578). The method had a limit of detection (LOD) (S/N=3) of 0.002 mg/kg (except for albendazole-2-aminosulfone, thiabendazole, and thiabendazole-5-hydroxy, which were 0.003 mg/kg). The limit of quantitation (LOQ) (S/N=10) was 0.005 mg/kg (except for albendazole-2-aminosulfone, thiabendazole, and thiabendazole-5-hydroxy, which were 0.010 mg/kg). The recoveries at three spiked levels of 0.005, 0.01 and 0.05 mg/kg ranged from 62.5% to 117.6%, with relative standard deviations (RSD) between 1.92% and 12.17%. The method is simple to operate, highly sensitive, and suitable for the quantitative analysis of benzimidazoles and their metabolites in dairy products.

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