Effect and Mechanism of <i>Armillaria mellea</i> 07-22 Fermentation on the Degradation of Zearalenone

WANG Zexian; ZHAO Yunan; JIA Dandan; JI Wantang; XU Ding; XIANG Yangling; CAI Dan; LIU Jingsheng

doi:10.13386/j.issn1002-0306.2023030070

Volume 45 Issue 1

Dec. 2023

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(1): 162-169. > DOI: 10.13386/j.issn1002-0306.2023030070

WANG Zexian, ZHAO Yunan, JIA Dandan, et al. Effect and Mechanism of Armillaria mellea 07-22 Fermentation on the Degradation of Zearalenone[J]. Science and Technology of Food Industry, 2024, 45(1): 162−169. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023030070.

Citation:

PDF (2000 KB)

Effect and Mechanism of Armillaria mellea 07-22 Fermentation on the Degradation of Zearalenone

College of Food Science and Engineering, Jilin Agricultural University, National Engineering Research Center for Deep Processing of Wheat and Corn, Changchun 130118, China

More Information

Received Date: March 05, 2023
Available Online: November 02, 2023

Graphical Abstract

Abstract

Abstract

This study used Armillaria mellea 07-22 as the experimental strain to degrade zearalenone (ZEN) by fungal biological fermentation. The degradation effects of Armillaria mellea on ZEN were studied, including the degradation effects of different concentrations of ZEN by the strain and the effects of different culture time, culture temperature, initial pH value and inoculation amount on the degradation of ZEN by the strain. Then the degradation mechanism was explored, the degradation effects of mycelium, fermentation supernatant and cell contents on ZEN were analyzed, and the effects of different fermentation time, pH values, and metal ions on degradation of ZEN by fermentation supernatant were studied, and the correlation between degradation effect and laccase production activity of the strain was illustrated. The results showed that Armillaria mellea 07-22 had a good degradation effect on ZEN. When the ZEN concentration was 5 μg/mL, the optimal degradation conditions were culture time of 8 days, culture temperature of 27 ℃, initial pH of 7.0, and inoculation amount of 10%. At this time, the degradation rate of ZEN was 78.72%. The degradation rates of ZEN by mycelium, fermentation supernatant and cell contents were 47.42%, 37.05% and 13.08% respectively. The extracellular enzymes secreted by Am-07-22 were the main way to degrade ZEN, and the mycelium cells also had a certain adsorption effect on ZEN. In addition, the correlation between the degradation rate of ZEN by fermentation supernatant and laccase activity was 0.973, and Cu²⁺ had the best promoting effect on the degradation of ZEN by fermentation supernatant.
- zearalenone,
- Armillaria mellea,
- mycotoxin,
- microbial degradation,
- degradation mechanism

FullText(HTML)

References (32)

References

[1]	WU N, OU W, ZHANG Z, et al. Recent advances in detoxification strategies for zearalenone contamination in food and feed[J]. Chinese Journal of Chemical Engineering,2021,30(2):168−177.
[2]	WANG J, XIE Y. Review on microbial degradation of zearalenone and aflatoxins[J]. Grain & Oil Science and Technology,2020,3(3):117−125.
[3]	HUEZA I M, RASPANTINI P C, RASPANTINI L E, et al. Zearalenone, an estrogenic mycotoxin, is an immunotoxic compound[J]. Toxins (Basel),2014,6(3):1080−1095. doi: 10.3390/toxins6031080
[4]	XU J, LI S, JIANG L, et al. Baicalin protects against zearalenone-induced chicks liver and kidney injury by inhibiting expression of oxidative stress, inflammatory cytokines and caspase signaling pathway[J]. International Immunopharmacology,2021,100(11):108097.
[5]	FLYNN K M, NEWBOLD R R, FERGUSON S A. Multigenerational exposure to dietary nonylphenol has no severe effects on spatial learning in female rats[J]. Neurotoxicology,2002,23(1):87−94. doi: 10.1016/S0161-813X(02)00007-4
[6]	KOWALSKA K, HABROWSKA-GÓRCZYŃSKA D E, DOMIŃSKA K, et al. The dose-dependent effect of zearalenone on mitochondrial metabolism, plasma membrane permeabilization and cell cycle in human prostate cancer cell lines[J]. Chemosphere,2017,180(8):455−466.
[7]	LU Q, LUO J Y, RUAN H N, et al. Structure-toxicity relationships, toxicity mechanisms and health risk assessment of food-borne modified deoxynivalenol and zearalenone:A comprehensive review[J]. Science of The Total Environment,2022,806(P3):151192.
[8]	ZHANG W, ZHANG L, JIANG X, et al. Enhanced adsorption removal of aflatoxin B1, zearalenone and deoxynivalenol from dairy cow rumen fluid by modified nano-montmorillonite and evaluation of its mechanism[J]. Animal Feed Science and Technology,2020,259(C):114366.
[9]	QU M, TIAN S, YU H, et al. Single-kernel classification of deoxynivalenol and zearalenone contaminated maize based on visible light imaging under ultraviolet light excitation combined with polarized light imaging[J]. Food Control,2023,144(2):109354.
[10]	MA C G, WANG Y D, HUANG W F, et al. Molecular reaction mechanism for elimination of zearalenone during simulated alkali neutralization process of corn oil[J]. Food Chemistry,2020,307(C):125546−125546.
[11]	赵仁勇, 宋斌. 玉米赤霉烯酮生物脱毒研究进展[J]. 河南工业大学学报(自然科学版),2018,39(2):113−121. [ZHAO R Y, SONG B. Advances in biological detoxification of zearalenone[J]. Journal of Henan University of Technology(Natural Science Edition),2018,39(2):113−121.] doi: 10.3969/j.issn.1673-2383.2018.02.020 ZHAO R Y, SONG B. Advances in biological detoxification of zearalenone[J]. Journal of Henan University of Technology（Natural Science Edition）, 2018, 39（2）: 113−121. doi: 10.3969/j.issn.1673-2383.2018.02.020
[12]	KOSAWANG C, KARLSSON M, VÉLËZ H, et al. Zearalenone detoxification by zearalenone hydrolase is important for the antagonistic ability of Clonostachys rosea against mycotoxigenic Fusarium graminearum[J]. Fungal Biology,2014,118(4):364−373. doi: 10.1016/j.funbio.2014.01.005
[13]	VEKIRU E, FRUHAUF S, HAMETNER C, et al. Isolation and characterisation of enzymatic zearalenone hydrolysis reaction products[J]. World Mycotoxin Journal,2016,9(3):1−12.
[14]	ZHAO L, JIN H, LAN J, et al. Detoxification of zearalenone by three strains of Lactobacillus plantarum from fermented food in vitro[J]. Food Control,2015,54(8):158−164.
[15]	XU J, WANG H, ZHU Z, et al. Isolation and characterization of Bacillus amyloliquefaciens ZDS-1:Exploring the degradation of zearalenone by Bacillus spp[J]. Food Control,2016,68(10):244−250.
[16]	GONZÁLEZ PEREYRA M L, DI GIACOMO A L, LARA A L, et al. Aflatoxin-degrading Bacillus sp. strains degrade zearalenone and produce proteases, amylases and cellulases of agro-industrial interest[J]. Toxicon,2020,180(C):43−48.
[17]	SUN X, HE X, XUE K S, et al. Biological detoxification of zearalenone by Aspergillus niger strain FS10[J]. Food and Chemical Toxicology,2014,72(10):76−82.
[18]	OKWARA P C, AFOLABI I S, AHUEKWE E F. Application of laccase in aflatoxin B1 degradation:A review[J]. IOP Conference Series:Materials Science and Engineering,2021,1107(1):012178−012189. doi: 10.1088/1757-899X/1107/1/012178
[19]	LOI M, FANELLI F, CIMMARUSTI M T, et al. In vitro single and combined mycotoxins degradation by Ery4 laccase from Pleurotus eryngii and redox mediators[J]. Food Control,2018,90(8):401−406.
[20]	SONG Y, WANG Y, GUO Y, et al. Degradation of zearalenone and aflatoxin B1 by Lac2 from Pleurotus pulmonarius in the presence of mediators[J]. Toxicon,2021,201(15):1−8.
[21]	何音华. 蜜环菌发酵玉米蛋白粉产物中活性蛋白分离纯化及功能活性研究[D]. 长春:吉林农业大学, 2018. [HE Y H. Separation, purification and function activities of active proteins from the fermentation products of Armillaria mellea[D]. Changchun:Jilin Agricultural Unversity, 2018.] HE Y H. Separation, purification and function activities of active proteins from the fermentation products of Armillaria mellea[D]. Changchun: Jilin Agricultural Unversity, 2018.
[22]	骆翼. 玉米赤霉烯酮的微生物脱毒研究[D]. 上海:上海交通大学, 2014. [LUO Y. Study of detoxification of zearalenone by Bacillus spp D]. Shanghai:Shanghai Jiaotong University, 2014.
[23]	金博文. 玉米赤霉烯酮降解菌株的分离、鉴定及降解特性的研究[D]. 大连:大连理工大学, 2020. [JIN B W. Isolation, identification and degradation of a zearalenone-degrading Sphingobacterium multivorum[D]. Dalian:Dalian University of Technology, 2020.] JIN B W. Isolation, identification and degradation of a zearalenone-degrading Sphingobacterium multivorum[D]. Dalian: Dalian University of Technology, 2020.
[24]	景思源. 玉米赤霉烯酮降解菌株的筛选及其降解效果研究[D]. 长春:吉林大学, 2021. [JING S Y. Screening of zearalenone degrading bacteria and its degradation effect[D]. Changchun:Jilin University, 2021.] JING S Y. Screening of zearalenone degrading bacteria and its degradation effect[D]. Changchun: Jilin University, 2021.
[25]	刘天睿, 张薇薇, 王忠巧, 等. 蜜环菌胞外酶和多糖含量变化规律研究[J]. 中药材,2019,42(1):57−61. [LIU T R, ZHANG W W, WANG Z Q, et al. Law of content change of extracellular enzymes and polysaccharides in Armillaria gallica[J]. Journal of Chinese Medicinal Materials,2019,42(1):57−61.] doi: 10.13863/j.issn1001-4454.2019.01.012 LIU T R, ZHANG W W, WANG Z Q, et al. Law of content change of extracellular enzymes and polysaccharides in Armillaria gallica[J]. Journal of Chinese Medicinal Materials, 2019, 42（1）: 57−61. doi: 10.13863/j.issn1001-4454.2019.01.012
[26]	韦锦范. 平菇漆酶菌株的筛选及其对黄曲霉毒素的降解研究[D]. 南宁:广西大学, 2019. [WEI J F. Screening of high-producing laccase Plurotus ostreatus strains and their degradation of aflatoxins[D]. Nanning:Guangxi University, 2019.] WEI J F. Screening of high-producing laccase Plurotus ostreatus strains and their degradation of aflatoxins[D]. Nanning: Guangxi University, 2019.
[27]	WANG Y Q, GUIQIN B I, ZHANG H L, et al. Screening of chlorobenzene-degrading bacteria and a study of their degrading performance[J]. Industrial Water & Wastewater,2003,34(6):35−36.
[28]	TAN H, ZHANG Z, HU Y, et al. Isolation and characterization of Pseudomonas otitidis TH-N1 capable of degrading zearalenone[J]. Food Control,2015,47(1):285−290.
[29]	IMADE F N, HUMZA M, DADA O A, et al. Isolation and characterization of novel soil bacterium, Klebsiella pneumoniae strain GS7-1 for the degradation of zearalenone in major cereals[J]. Food Control,2023,143(1):109287−109287.
[30]	BANU I, LUPU A, APRODU I. Degradation of zearalenone by laccase enzyme[J]. Scientific Study & Research Chemistry & Chemical Engineering Biotechnology Food Industry,2014,14(2):79−84.
[31]	白长胜, 刘秋瑾, 尹珺伊, 等. 产木质纤维素降解酶真菌的筛选及产酶特性[J]. 微生物学通报,2023,50(3):1098−1110. [BAI C S, LIU Q J, YIN J Y, et al. Screening and enzymatic characterization of the fungal strains producing lignocellulose-degrading enzymes[J]. Microbiology China,2023,50(3):1098−1110.] doi: 10.13344/j.microbiol.china.220632 BAI C S, LIU Q J, YIN J Y, et al. Screening and enzymatic characterization of the fungal strains producing lignocellulose-degrading enzymes[J]. Microbiology China, 2023, 50（3）: 1098−1110. doi: 10.13344/j.microbiol.china.220632
[32]	祝嫦巍, 鲍广稳, 黄顺. 平菇漆酶对不同重金属胁迫的响应[J]. 环境科学研究,2015,28(10):1631−1637. [ZHU C W, BAO G W, HUANG S. Change in laccase activities of Pleurotus ostreatus in response to heavy metals in liquid culture[J]. Research of Environmental Sciences,2015,28(10):1631−1637.] doi: 10.13198/j.issn.1001-6929.2015.10.19 ZHU C W, BAO G W, HUANG S. Change in laccase activities of Pleurotus ostreatus in response to heavy metals in liquid culture[J]. Research of Environmental Sciences, 2015, 28（10）: 1631−1637. doi: 10.13198/j.issn.1001-6929.2015.10.19