Research on the Differences in Bacteria Community Structure and Gene Function of Medium-High Temperature Daqu in Linyi and Zhoukou Areas

MA Mengyue; GUO Zhuang; LI Xuesi; GUAN Guikun; LIU Yu; LI Fuqiang; WANG Yurong

doi:10.13386/j.issn1002-0306.2023030055

Volume 45 Issue 1

Dec. 2023

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Science and Technology of Food Industry > 2024 > 45(1): 155-161. > DOI: 10.13386/j.issn1002-0306.2023030055

MA Mengyue, GUO Zhuang, LI Xuesi, et al. Research on the Differences in Bacteria Community Structure and Gene Function of Medium-High Temperature Daqu in Linyi and Zhoukou Areas[J]. Science and Technology of Food Industry, 2024, 45(1): 155−161. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023030055.

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Research on the Differences in Bacteria Community Structure and Gene Function of Medium-High Temperature Daqu in Linyi and Zhoukou Areas

1.
Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China
2.
College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
3.
Shandong Lanling Meijiu Co., Ltd., Lanling 277731, China
4.
Henan Songhe Wine Co., Ltd., Zhoukou 477262, China

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Received Date: March 05, 2023
Available Online: November 02, 2023

Graphical Abstract

Abstract

Abstract

In order to clarify the differences in the community structure and gene function of medium-high temperature Daqu bacteria in Linyi, Shandong Province and Zhoukou, Henan Province. MiSeq high-throughput sequencing technology was used to analyze the bacterial community structure of medium-high temperature Daqu in the two regions, and the potential functions of bacterial taxa were analyzed based on the clusters of orthologous groups of proteins (COG). The result of α diversity showed that there were no significant differences in richness and diversity (P>0.05). Compared with Daqu in Zhoukou area, at the phylum level, the average relative abundance of Actinobacteria and Bacteroidetes in Daqu in Linyi area were significantly lower (P<0.05). At the genus level, the average relative abundance of Acinetobacter was significantly higher (P<0.05), and the average relative abundance of Geobacter and Pantoea were significantly lower (P<0.05). The result of linear discriminant analysis effect size (LEfSe) showed that Geobacter was a biomarker in Zhoukou area Daqu, while the biomarker in Linyi area Daqu was not reflected at the genus level. The result of β diversity showed that there were obvious differences as a whole. The result showed that Daqu in Linyi area was significantly higher than Daqu in Zhoukou area in transcription, amino acid and nucleoside transport and metabolic functions by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) (P<0.05). Thus, there are obvious differences in bacterial community structure and gene function expression in the two regions.
- region,
- medium-high temperature Daqu,
- bacterial diversity,
- high-throughput sequencing,
- gene function

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