Optimization and Quality Evaluation of Rape Bee Pollen Jiaosu Fermentation by <i>Candida apicola</i> LGL-2

LIAO Xiang; DENG Junxia; BAI Weidong; XIAO Gengsheng; LAI Qiuping; WANG Hong; LIU Gongliang

doi:10.13386/j.issn1002-0306.2023020277

Volume 44 Issue 24

Dec. 2023

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Science and Technology of Food Industry > 2023 > 44(24): 159-167. > DOI: 10.13386/j.issn1002-0306.2023020277

LIAO Xiang, DENG Junxia, BAI Weidong, et al. Optimization and Quality Evaluation of Rape Bee Pollen Jiaosu Fermentation by Candida apicola LGL-2[J]. Science and Technology of Food Industry, 2023, 44(24): 159−167. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020277.

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Optimization and Quality Evaluation of Rape Bee Pollen Jiaosu Fermentation by Candida apicola LGL-2

1.
College of Light industry and Food Engineering, Zhongkai University of Agriculture and Engineering, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Guangzhou 510225, China
2.
Guangdong Guiling Bees Industry Science and Technology Co., Ltd., Meizhou 514199, China

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Received Date: February 27, 2023
Available Online: October 09, 2023

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Abstract

Abstract

Using rape bee pollen as raw material, the Candida apicola LGL-2 selected from honey was used to investigate the effect of fermentation factors including fermentation time, fermentation temperature, inoculum amount and substrate content on the total phenolic content of rape bee pollen jiaosu. The fermentation process of rape bee pollen jiaosu was optimized by response surface methodology (RSM) using total phenolic content as the response variable. Based on these conditions, the phenolic compounds, volatile aroma components and antioxidant activities of rape bee pollen and jiaosu were further compared. The results showed that the optimal conditions for rape bee pollen jiaosu by Candida apicola LGL-2 were fermentation time 48 h, fermentation temperature 30 ℃, inoculum amount 20% and substrate content 30%, and the total phenolic content of rape bee pollen jiaosu was 11.48 mg GAE/g after fermentation. Phenolic compounds including gallic acid, p-coumaric acid, rutin, ellagic acid and quercetin were detected in rape bee pollen and jiaosu by high performance liquid chromatography (HPLC), and the contents of all the phenolic compounds above were increased after the fermentation by Candida apicola LGL-2. The volatile aroma compounds of rape bee pollen and jiaosu were analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) combined with the relative odor activity value (ROAV). Twenty-six volatile aroma compounds were detected, 7 of which were identified as key aroma components of rape bee pollen jiaosu, and the contents of some alcohols and esters were enhanced to bring excellent flavor to jiaosu. Compared with rape bee pollen, rape bee pollen jiaosu exhibited stronger antioxidant activities as determined by DPPH·, ABTS⁺· and FRAP assay. These results indicated that fermentation by Candida apicola LGL-2 could effectively improve the phenolic content, flavor and antioxidant power of rape bee pollen.
- rape bee pollen,
- jiaosu,
- phenolic compounds,
- flavor,
- Candida apicola LGL-2

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References

[1]	任向楠, 张红城, 董捷. 蜂花粉破壁的研究进展[J]. 食品科学,2009,30(21):380−383. [REN X N, ZHANG H C, DONG J. Research progress in cell disruption techniques for pollen[J]. Food Science,2009,30(21):380−383.] REN X N, ZHANG H C, DONG J. Research progress in cell disruption techniques for pollen[J]. Food Science, 2009, 30（21）: 380−383.
[2]	杨佳林, 徐响, 孙丽萍, 等. 蜂花粉酚类化合物研究进展[J]. 食品科学,2008(8):693−696. [YANG J L, XU X, SUN L P, et al. Research progress of bee pollen phenolic compounds[J]. Food Science,2008(8):693−696.] YANG J L, XU X, SUN L P, et al. Research progress of bee pollen phenolic compounds[J]. Food Science, 2008（8）: 693−696.
[3]	URCAN A C, CRISTE A D, DEZMIREAN D S, et al. Similarity of data from bee bread with the same taxa collected in India and Romania[J]. Molecules,2018,23(10):2491. doi: 10.3390/molecules23102491
[4]	胡东辉. 油菜花粉对前列腺增生的临床治疗[J]. 中国医药导报,2009,6(13):251. [HU D H. Clinical treatment of prostatic hyperplasia with rape bee pollen[J]. China Medical Herald,2009,6(13):251.] doi: 10.3969/j.issn.1673-7210.2009.13.164 HU D H. Clinical treatment of prostatic hyperplasia with rape bee pollen[J]. China Medical Herald, 2009, 6（13）: 251. doi: 10.3969/j.issn.1673-7210.2009.13.164
[5]	章振东. 蜂花粉篇[J]. 中国蜂业,2019,70(5):19. [ZHANG Z D. Bee pollen[J]. Apiculture of China,2019,70(5):19.] doi: 10.3969/j.issn.0412-4367.2019.05.006 ZHANG Z D. Bee pollen[J]. Apiculture of China, 2019, 70（5）: 19. doi: 10.3969/j.issn.0412-4367.2019.05.006
[6]	DAI J, SHA R, WANG Z, et al. Edible plant Jiaosu:Manufacturing, bioactive compounds, potential health benefits, and safety aspects[J]. J Sci Food Agric,2020,100(15):5313−5323. doi: 10.1002/jsfa.10518
[7]	苏春雷, 王强, 黄洁君, 等. 新型余甘子酵素发酵工艺的优化[J]. 食品与发酵工业,2019,45(9):128−136. [SU C L, WANG Q, HUANG J J, et al. Optimized fermentation process to produce novel phyllanthus emblica jiaosu[J]. Food and Fermentation Industries,2019,45(9):128−136.] SU C L, WANG Q, HUANG J J, et al. Optimized fermentation process to produce novel phyllanthus emblica jiaosu[J]. Food and Fermentation Industries, 2019, 45（9）: 128−136.
[8]	TOHAMY A A, ABDELLA E M, AHMED R R, et al. Assessment of anti-mutagenic, antihistopathologic and antioxidant capacities of Egyptian bee pollen and propolis extracts[J]. Cytotechnology,2014,66(2):283−297. doi: 10.1007/s10616-013-9568-0
[9]	王印壮, 段定定, 丁玉峰, 等. 葡萄酵素发酵过程中代谢产物的动态变化[J]. 食品科学,2022,43(18):98−104. [WANG Y Z, DUANG D D, DING Y F, et al. Dynamic changes of metabolites during grape jiaosu fermentation[J]. Food Science,2022,43(18):98−104.] WANG Y Z, DUANG D D, DING Y F, et al. Dynamic changes of metabolites during grape jiaosu fermentation[J]. Food Science, 2022, 43（18）: 98−104.
[10]	YAN S, LI Q, XUE X, et al. Analysis of improved nutritional composition of bee pollen (Brassica campestris L.) after different fermentation treatments[J]. International Journal of Food Science & Technology, 2019, 54(6).
[11]	郑慧, 杨思琪, 孙艳, 等. 油菜蜂花粉破壁技术研究进展[J]. 食品与机械,2021,37(8):231−238. [ZHNEG H, YANG S Q, SUN Y, et al. Research progress on wall-breaking technology of rape bee pollen[J]. Food & Machinery,2021,37(8):231−238.] ZHNEG H, YANG S Q, SUN Y, et al. Research progress on wall-breaking technology of rape bee pollen[J]. Food & Machinery, 2021, 37（8）: 231−238.
[12]	张敏倩, 刘功良, 费永涛, 等. 利用蜂蜜接合酵母合成海藻糖[J]. 食品与发酵工业,2021,47(3):107−113. [ZHANG M Q, LIU G L, FEI Y T, et al. Synthesis of trehalose byZygosaccharomyces mellis[J]. Food and Fermentation Industries,2021,47(3):107−113.] ZHANG M Q, LIU G L, FEI Y T, et al. Synthesis of trehalose by Zygosaccharomyces mellis[J]. Food and Fermentation Industries, 2021, 47（3）: 107−113.
[13]	王小超, 程妮, 段倩倩, 等. 体外消化和结肠发酵对蜂花粉酚类化合物及抗氧化活性的影响[J]. 食品与发酵工业,2022,48(6):141−146. [WANG X C, CHENG N, DUANG Q Q, et al. Effects of digestion and colonic fermentation in vitro on phenolic compounds and antioxidant activities of bee pollen[J]. Food and Fermentation Industries,2022,48(6):141−146.] WANG X C, CHENG N, DUANG Q Q, et al. Effects of digestion and colonic fermentation in vitro on phenolic compounds and antioxidant activities of bee pollen[J]. Food and Fermentation Industries, 2022, 48（6）: 141−146.
[14]	田文礼, 孙丽萍, 董捷, 等. Folin-Ciocaileu比色法测定蜂花粉中的总酚[J]. 食品科学,2007(2):258−260. [TIAN W L, SUN L P, DONG J, et al. Determination of total polyphenolic compounds in bee-pollen by Folin-Ciocaileu[J]. Food Science,2007(2):258−260.] TIAN W L, SUN L P, DONG J, et al. Determination of total polyphenolic compounds in bee-pollen by Folin-Ciocaileu[J]. Food Science, 2007（2）: 258−260.
[15]	徐元元, 王悦, 杨二林, 等. 3种蜂花粉酚类化合物组成及抗氧化活性研究[J]. 西北大学学报(自然科学版),2021,51(2):303−313. [XU Y Y, WANG Y, YANG E L, et al. Phenolic composition and antioxidant activities of three kinds of bee pollen[J]. Journal of Northwest University (Natural Science Edition),2021,51(2):303−313.] XU Y Y, WANG Y, YANG E L, et al. Phenolic composition and antioxidant activities of three kinds of bee pollen[J]. Journal of Northwest University （Natural Science Edition）, 2021, 51（2）: 303−313.
[16]	程娅娅. 花粉蜂蜜发酵乳的研制[D]. 扬州:扬州大学, 2016. [CHENG Y Y. Study on bee pollen fermented milk[D]. Yangzhou:Yangzhou University, 2016.] CHENG Y Y. Study on bee pollen fermented milk[D]. Yangzhou: Yangzhou University, 2016.
[17]	刘登勇, 周光宏, 徐幸莲. 确定食品关键风味化合物的一种新方法:“ROAV”法[J]. 食品科学,2008(7):370−374. [LIU D Y, ZHOU G H, XU X L. "ROAV" Method:A new method for determining key odor compounds of Rugao Ham[J]. Food Science,2008(7):370−374.] LIU D Y, ZHOU G H, XU X L. "ROAV" Method: A new method for determining key odor compounds of Rugao Ham[J]. Food Science, 2008（7）: 370−374.
[18]	郑利琴, 张慜, 孙金才, 等. 微波与超声波提取杨梅汁多酚类物质的对比研究[J]. 食品与生物技术学报,2010,29(4):514−520. [ZHENG L Q, ZHANG M, SUN J C, et al. Comparison study on the microwave and ultrasonic wave extraction polyphenol from bayberry juice[J]. Journal of Food Science and Biotechnology,2010,29(4):514−520.] ZHENG L Q, ZHANG M, SUN J C, et al. Comparison study on the microwave and ultrasonic wave extraction polyphenol from bayberry juice[J]. Journal of Food Science and Biotechnology, 2010, 29（4）: 514−520.
[19]	KAPRASOB R, KERDCHOECHUEN O, LAOHAKUNJIT N, et al. Changes in physico-chemical, astringency, volatile compounds and antioxidant activity of fresh and concentrated cashew apple juice fermented with Lactobacillus plantarum[J]. J Food Sci Technol,2018,55(10):3979−3990. doi: 10.1007/s13197-018-3323-7
[20]	张笑莹, 赵江丽, 李月, 等. 雪花梨酵素二步发酵工艺优化[J]. 现代食品科技,2023,39(7):32−41. [ZHANG X Y, ZHAO J L, LI Y, et al. Process optimization of two-step fermentation process of xuehua pear ferment[J]. Modern Food Science and Technology,2023,39(7):32−41.] ZHANG X Y, ZHAO J L, LI Y, et al. Process optimization of two-step fermentation process of xuehua pear ferment[J]. Modern Food Science and Technology, 2023, 39（7）: 32−41.
[21]	YIKMIŞ S. Optimization of uruset apple vinegar production using response surface methodology for the enhanced extraction of bioactive substances[J]. Foods, 2019, 8(3):107.
[22]	MĂRGĂOAN R, STRANȚ M, VARADI A, et al. Bee collected pollen and bee bread:bioactive constituents and health benefits[J]. Antioxidants, 2019, 8(12):568.
[23]	ZHANG Y, YANG F, JAMALI M A, et al. Antioxidant enzyme activities and lipid oxidation in rape (Brassica campestris L.) bee pollen added to salami during processing[J]. Molecules,2016,21(11):1439. doi: 10.3390/molecules21111439
[24]	肖兴英, 乔江涛, 张红城. 破壁前后油菜蜂花粉中黄酮类化合物定性定量分析[J]. 中国蜂业,2021,72(7):60−64. [XIAO X Y, QIAO J T, ZHANG H C. Qualitative and quantitative analysis of flavonoids in rape bee pollen before and after wall disruption[J]. Apiculture of China,2021,72(7):60−64.] XIAO X Y, QIAO J T, ZHANG H C. Qualitative and quantitative analysis of flavonoids in rape bee pollen before and after wall disruption[J]. Apiculture of China, 2021, 72（7）: 60−64.
[25]	SHAHIDI F, YEO J D. Insoluble-bound phenolics in food[J]. Molecules, 2016, 21(9).
[26]	YAN S B, CHEN X S, XIANG X B. Improvement of the aroma of lily rice wine by using aroma-producing yeast strain Wickerhamomyces anomalus HN006[J]. AMB Express,2019,9(1):89. doi: 10.1186/s13568-019-0811-8
[27]	吴昊远. 油松松花粉乳饮料的研制和品质研究[D]. 泰安:山东农业大学, 2020. [WU H Y. Research on the development and quality study of pine pollen[D]. Taian:Shandong Agricultural University, 2020.] WU H Y. Research on the development and quality study of pine pollen[D]. Taian: Shandong Agricultural University, 2020.
[28]	BLEVE G, TUFARIELLO M, DURANTE M, et al. Physico-chemical and microbiological characterization of spontaneous fermentation of Cellina di Nardò and Leccino table olives[J]. Front Microbiol,2014,5:570.
[29]	CONNOR M R, LIAO J C. Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol[J]. Appl Environ Microbiol,2008,74(18):5769−5775. doi: 10.1128/AEM.00468-08
[30]	CHOI S M, LEE D J, KIM J Y, et al. Volatile composition and sensory characteristics of onion powders prepared by convective drying[J]. Food Chemistry,2017,231:386−392. doi: 10.1016/j.foodchem.2017.03.129
[31]	FANG Y, GU S, ZHANG J, et al. Deodorisation of fish oil by nanofiltration membrane process:Focus on volatile flavour compounds and fatty acids composition[J]. International Journal of Food Science & Technology,2018,53(3):692−699.
[32]	PATTON S. Flavor thresholds of volatile fatty acids[J]. Journal of Food Science, 1964, 29(5):679-680.
[33]	文彦. 形成葡萄酒香气特征的关键香气成分及其呈香机制研究[D]. 杨凌:西北农林科技大学, 2013. [WEN Y. Study on the impact odorants of wine and the formation of wine aroma characteristics[D]. Yangling:Northwest A&F University, 2013.] WEN Y. Study on the impact odorants of wine and the formation of wine aroma characteristics[D]. Yangling: Northwest A&F University, 2013.
[34]	MO E K, SUNG C K. Phenylethyl alcohol (PEA) application slows fungal growth and maintains aroma in strawberry[J]. Postharvest Biology and Technology,2007,45(2):234−239. doi: 10.1016/j.postharvbio.2007.02.005
[35]	蔡秋萍. 四种蜂产品中挥发性成分分析及主要香气成分的鉴定[D]. 湛江:广东海洋大学, 2021. [CAI Q P. Analysis of volatile components and identification of main aroma components in four kinds of bee products[D]. Zhanjiang:Guangdong Ocean University, 2021.] CAI Q P. Analysis of volatile components and identification of main aroma components in four kinds of bee products[D]. Zhanjiang: Guangdong Ocean University, 2021.
[36]	AYLANC V, TOMÁS A, RUSSO-ALMEIDA P, et al. Assessment of bioactive compounds under simulated gastrointestinal digestion of bee pollen and bee bread:Bioaccessibility and antioxidant activity[J]. Antioxidants (Basel), 2021, 10(5):651.
[37]	KING A J, GRIFFIN J K, ROSLAN F. In vivo and in vitro addition of dried olive extract in poultry[J]. J Agric Food Chem,2014,62(31):7915−7919. doi: 10.1021/jf4050588

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