Electrochemical Aptasensor Based on Platinum @ Gold Nanowires as Signal Amplifier for Aflatoxin B1 Detection

LIU Juntao; LIU Zhijing; LI Xiaojiang; MA Zhiwei

doi:10.13386/j.issn1002-0306.2023020181

Volume 44 Issue 24

Dec. 2023

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Science and Technology of Food Industry > 2023 > 44(24): 294-300. > DOI: 10.13386/j.issn1002-0306.2023020181

LIU Juntao, LIU Zhijing, LI Xiaojiang, et al. Electrochemical Aptasensor Based on Platinum @ Gold Nanowires as Signal Amplifier for Aflatoxin B1 Detection[J]. Science and Technology of Food Industry, 2023, 44(24): 294−300. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020181.

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Electrochemical Aptasensor Based on Platinum @ Gold Nanowires as Signal Amplifier for Aflatoxin B1 Detection

Faculty of Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450000, China

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Received Date: February 19, 2023
Available Online: October 16, 2023

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Abstract

Abstract

Objective: In this study, to meet food quality and safety and rapid determination of aflatoxin B1 (AFB1) content in food, an electrochemical aptamer-based sensor with plantium@gold nanowires (Pt@AuNWs) as the signal amplifier was developed for sensitive and selective detection of AFB1 in peanut. Method: Firstly, Au nanoparticles (AuNPs) were deposited on the surface of glassy carbon electrode (AuNPs/GC) for enhancing the electrocatalytic property of the sensor and linking complementary strand (cDNA) through Au-S bond. Then, the signal probes was constructed via template synthesis of Pt@AuNWs and combination with aptamer (Pt@AuNPs-apt), which could bind to the electrode based on base complementary pairing principle. In the presence of AFB1, it would compete with cDNA to bind aptamer and result in the detachment of some signal probes from the sensor surface, reducing the electrical signals generated by catalyzing H₂O₂, thereby the indirectly quantitative detection of AFB1 was achieved. Result: The sensor showed the best analytical performance with 0.25 nmol/L cDNA, incubating apt and cDNA for 40 min, 10 mmol/L H₂O₂, 0.1 mol/L pH7.0 PBS. Under optimization, this sensor showed good lineariy in the range of 1~100 ng/mL,with the calculated detection limit about 0.41 ng/mL. The present sensor delivered good sensitivity, selectivity and stability. The method was applied to the peanut samples for the recovery experiment and the recovery rate was 85.1%~88.0%. Conclusion: The constructed electrochemical adapter sensor can be applied to the detection of AFB1 in peanuts, and has great potential to be used in rapid detection.
- platinum @ gold nanowires,
- aptamer,
- electrochemical aptasensor,
- aflatoxin B1

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References

[1]	WANG C, ZHAO Q. A reagentless electrochemical sensor for aflatoxin B1 with sensitive signal-on responses using aptamer with methylene blue label at specific internal thymine[J]. Biosensors and Bioelectronics,2020,167:112478−112483. doi: 10.1016/j.bios.2020.112478
[2]	XU Z, LONG L L, CHEN Y Q, et al. A nanozyme-linked immunosorbent assay based on metal–organic frameworks (MOFs) for sensitive detection of aflatoxin B1[J]. Food Chemistry,2021,338:128039−128045. doi: 10.1016/j.foodchem.2020.128039
[3]	QIAN J, REN C C, WANG C Q, et al. Gold nanoparticles mediated designing of versatile aptasensor for colorimetric/electrochemical dual-channel detection of aflatoxin B1[J]. Biosensors and Bioelectronics,2020,166:112443−112450. doi: 10.1016/j.bios.2020.112443
[4]	LI S Q, ZHONG X Y, XU Y N, et al. Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs[J]. [J]. Food Chemistry,2021,360:130019. doi: 10.1016/j.foodchem.2021.130019
[5]	SUN C N, LIAO X F, JIA B Y, et al. Development of a ZnCdS@ZnS quantum dots-based label-free electrochemiluminescence immunosensor for sensitive determination of aflatoxin B1 in lotus seed[J]. Microchimica Acta,2020,187(4):236−244. doi: 10.1007/s00604-020-4179-x
[6]	JIANG S, ZHANG L X, LI J Z, et al. Pressure/colorimetric dual-readout immunochromatographic test strip for point-of-care testing of aflatoxin B1[J]. Talanta,2021,227:122207−122213. doi: 10.1016/j.talanta.2021.122207
[7]	BU T, BAI F E, SUN X Y, et al. An innovative prussian blue nanocubes decomposition-assisted signal amplification strategy suitable for competitive lateral flow immunoassay to sensitively detect aflatoxin B1[J]. Food Chemistry,2021,344:1287111−1287118.
[8]	GELL R M, CARBONE I. HPLC quantitation of aflatoxin B1 from fungal mycelium culture[J]. Journal of Microbiological Methods,2019,158:14−17. doi: 10.1016/j.mimet.2019.01.008
[9]	ALGAMMAL A M, ELSAYED M E, HASHEM H R, et al. Molecular and HPLC-based approaches for detection of aflatoxin B1 and ochratoxin A released from toxigenic Aspergillus species in processed meat[J]. BMC Microbiology,2021,21(1):82−91. doi: 10.1186/s12866-021-02144-y
[10]	ZHANG B, YU L, LIU Z, et al. Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high-performance liquid chromatography[J]. Journal of Separation Science,2020,43(17):3509−3519. doi: 10.1002/jssc.202000293
[11]	DENG Y J, WANG Y L, DENG Q, et al. Simultaneous quantification of aflatoxin B1, T-2 toxin, ochratoxin A and deoxynivalenol in dried seafood products by LC-MS/MS[J]. Toxins,2020,12(8):12,488−500.
[12]	WANG C Q, ZHANG W H, QIAN J, et al. A FRET aptasensor for sensitive detection of aflatoxin B1 based on a novel donor-acceptor pair between ZnS quantum dots and Ag nanocubes[J]. Analytical Methods,2021,13(4):462−468. doi: 10.1039/D0AY02017F
[13]	CHEN H F, HAN F, MAO B N, et al. Rapid and label free detection of aflatoxin B1 in alcoholic beverages with a microfluid fiber device[J]. Applied Optics,2021,60(7):1924−1929. doi: 10.1364/AO.414332
[14]	WANG N, LIU Q Q, HU X F, et al. Electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for rapid detection of aflatoxin B1 in peanut oil[J]. Analytical Biochemistry,2022,650:114710. doi: 10.1016/j.ab.2022.114710
[15]	GUAN Y, SI P B, YANG T, et al. A novel method for detection of ochratoxin A in foods-Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker[J] Food Chemistry, 2023, 403:134316.
[16]	ZHANG K, WANG Y, WANG H Y, et al. Three-dimensional porous reduced graphene oxide modified electrode for highly sensitive detection of trace rifampicin in milk[J]. Analytical Methods,2022,14(23):2304−2310. doi: 10.1039/D2AY00517D
[17]	ZHU C X, LIU X H, LI Y Y, et al. Dual-ratiometric electrochemical aptasensor based on carbon nanohorns/anthraquinone-2-carboxylic acid/Au nanoparticles for simultaneous detection of malathion and omethoate[J]. Talanta,2023,253:123966. doi: 10.1016/j.talanta.2022.123966
[18]	PATIL J J, REESE M L, LEE E, et al. Oxynitride-encapsulated silver nanowire transparent electrode with enhanced thermal, electrical, and chemical stability[J]. ACS Applied Materials & Interfaces,2022,14(3):4423−4433.
[19]	PARVIN S, KUMAR A, GHOSH A, et al. An earth-abundant bimetallic catalyst coated metallic nanowire grown electrode with platinum-like pH-universal hydrogen evolution activity at high current density[J]. Chemical Science,2020,11(15):3893−3902. doi: 10.1039/D0SC00754D
[20]	LI W, DENG X, WU Z Y, et al. An electrochemical sensor for quantitation of the oral health care agent chlorogenic acid based on bimetallic nanowires with functionalized reduced graphene oxide nanohybrids[J]. ACS Omega,2022,7(5):4614−4623. doi: 10.1021/acsomega.1c06612
[21]	SONG D D, LI Q, LU X, et al. Ultra-thin bimetallic alloy nanowires with porous architecture/monolayer MoS₂ nanosheet as a highly sensitive platform for the electrochemical assay of hazardous omethoate pollutant[J]. Journal of Hazardous Materials,2018,357:466−474. doi: 10.1016/j.jhazmat.2018.06.021
[22]	SHETTY S, GAYEN M, AGARWAL S, et al. Tuning catalytic activity in ultrathin bimetallic nanowires via surface segregation:some insights[J]. The Journal of Physical Chemistry Letters,2022,13(3):770−776. doi: 10.1021/acs.jpclett.1c03852
[23]	WANG H, JIAO S, LIU S, et al. Mesoporous bimetallic au@rh core-shell nanowires as efficient electrocatalysts for ph-universal hydrogen evolution[J]. ACS Applied Materials & Interfaces,2021,13(26):30479−30485.
[24]	SHARMA A S, ALI S, SABARINATHAN D, et al. Recent progress on graphene quantum dots-based fluorescence sensors for food safety and quality assessment applications[J]. Comprehensive Reviews in Food Science and Food Safety,2021,20(6):5765−5801. doi: 10.1111/1541-4337.12834
[25]	SUN J, LI L, GE S, et al. Dual-mode aptasensor assembled by a Wo₃/Fe₂O₃ heterojunction for paper-based colorimetric prediction/photoelectrochemical multicomponent analysis[J]. ACS Applied Materials & Interfaces,2021,13(3):3645−3652.
[26]	WEI H, BU S, ZHANG W, et al. An electrochemical biosensor for the detection of pathogenic bacteria based on dual signal amplification of Cu₃(PO₄)²⁻ mediated click chemistry and DNAzymes[J]. Analyst,2021,146(15):4841−4847. doi: 10.1039/D1AN00982F
[27]	JIANG Z W, ZHAO T T, LI C M, et al. 2D mof-based photoelectrochemical aptasensor for sars-cov-2 spike glycoprotein detection[J]. ACS Applied Materials & Interfaces,2021,13(42):49754−49761.
[28]	ZHANG B, LU Y, YANG C, et al. Simple "signal-on" photoelectrochemical aptasensor for ultrasensitive detecting AFB1 based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au nanocomposites[J]. Biosensors & Bioelectronics,2019,134:42−48.
[29]	ZHU L, ZHAO Y, YAO S L, et al. A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins[J]. Analyst,2021,146(8):2712−2717. doi: 10.1039/D1AN00251A
[30]	PAN L M, ZHAO X, WEI X, et al. Ratiometric luminescence aptasensor based on dual-emissive persistent luminescent nanoparticles for autofluorescence- and exogenous interference-free determination of trace aflatoxin B1 in food samples[J]. Analytical Chemistry,2022,94(16):6387−6393. doi: 10.1021/acs.analchem.2c00861
[31]	HONG W, WANG J, WANG E K. Dendritic Au/Pt and Au/PtCu nanowires with enhanced electrocatalytic ctivity for methanol electrooxidation[J]. Small,2014,10(16):3262−3265. doi: 10.1002/smll.201400059
[32]	YANG Y, CHEN M, WU Y J, et al. Ultrasound assisted one-step synthesis of Au@Pt dendritic nanoparticles with enhanced NIR absorption for photothermal cancer therapy[J]. RSC Advances 2019, 9 (49):28541−28547.