Screening and Identification of Selenium-tolerant Marine Strain and the Antibacterial Activity of Se Nanoparticles It Synthesized

XU Xiaonan; MA Haodi; XU Yan; LI Xuan; QIN Zhi; QUAN Chunshan; ZHANG Liying

doi:10.13386/j.issn1002-0306.2023020155

Volume 44 Issue 24

Dec. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(24): 152-158. > DOI: 10.13386/j.issn1002-0306.2023020155

XU Xiaonan, MA Haodi, XU Yan, et al. Screening and Identification of Selenium-tolerant Marine Strain and the Antibacterial Activity of Se Nanoparticles It Synthesized[J]. Science and Technology of Food Industry, 2023, 44(24): 152−158. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020155.

Citation:

PDF (3001 KB)

Screening and Identification of Selenium-tolerant Marine Strain and the Antibacterial Activity of Se Nanoparticles It Synthesized

XU Xiaonan^1,,
MA Haodi^{1, 2},
XU Yan¹,
LI Xuan¹,
QIN Zhi¹,
QUAN Chunshan^{1, 2, ,},
ZHANG Liying^{1, 2, ,}

1.
College of Life Sciences, Dalian Minzu University, Dalian 116600, China
2.
Key Laboratory of Biotechnology and Resource Utilization, Ministry of Education, Dalian 116600, China

More Information

Received Date: February 15, 2023
Available Online: October 08, 2023

Graphical Abstract

Abstract

Abstract

In this study, a novel strain with high Na₂SeO₃ reducing ability was screened from marine strains, providing a new reduction system for the biosynthesis of Se nanoparticles (SeNPs). Firstly, 41 marine strains from Arctic Ocean sediments were screened, and a strain with high selenium-tolerant was obtained and species identified. Subsequently, the screened marine strain was used for the biosynthesis SeNPs and the SeNPs were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and particle size analysis. Furthermore, the antibacterial activities of SeNPs were also determined. The results showed that strain 72 had the strongest tolerance to Na₂SeO₃, which could grow in the presence of 300 mmol/L Na₂SeO₃ and reducing it to brick red SeNPs. Subsequently, strain 72 was identified and named as Bacillus sp. Q72. The SeNPs biosynthesized by this strain were spherical particles with an average size of 169.3 nm and a potential of −48.1±0.5 mV. There were amino and hydroxyl groups on their surface. The SeNPs produced by Bacillus sp. Q72 showed better inhibitory activity against E. coli. When the concentration of SeNPs was 200 μg/mL, the inhibitory rate against E. coli reached 92.1%.
- marine strains,
- Se nanoparticles,
- biosynthesis,
- antibacterial activity,
- screening,
- identify

FullText(HTML)

References (39)

References

[1]	薛冰, 谢超新, 程水源, 等. 微生物转化法制备纳米硒及有机硒的研究进展[J]. 食品科技,2022,47(10):31−37. [XUE Bing, XIE Chaoxin, CHENG Shuiyuan, et al. Research progress in the preparation of nano-selenium and organic selenium by microbial transformation[J]. Food Science and Technology,2022,47(10):31−37.] XUE Bing, XIE Chaoxin, CHENG Shuiyuan, et al. Research progress in the preparation of nano-selenium and organic selenium by microbial transformation[J]. Food Science and Technology, 2022, 47（10）: 31−37.
[2]	苏华华, 王艳华. 纳米硒的制备及生物医学应用研究进展[J]. 生物技术通讯,2020,31(5):621−626. [SU Huahua, WANG Yanhua. Preparation of nanoselenium and research progress in biomedical applications[J]. Biotechnology Communications,2020,31(5):621−626.] SU Huahua, WANG Yanhua. Preparation of nanoselenium and research progress in biomedical applications[J]. Biotechnology Communications, 2020, 31（5）: 621−626.
[3]	王振宇, 魏凌峰, 蔡杰, 等. 纳米硒的合成研究及其在食品与农业中的应用[J]. 食品科技,2021,46(12):26−33. [WANG Zhenyu, WEI Lingfeng, CAI Jie, et al. Synthesis of nanoselenium and its application in food and agriculture[J]. Food Science and Technology,2021,46(12):26−33.] doi: 10.3969/j.issn.1005-9989.2021.12.spkj202112006 WANG Zhenyu, WEI Lingfeng, CAI Jie, et al. Synthesis of nanoselenium and its application in food and agriculture[J]. Food Science and Technology, 2021, 46（12）: 26−33. doi: 10.3969/j.issn.1005-9989.2021.12.spkj202112006
[4]	苏文, 杨辉, 董腾达, 等. 纳米硒制备方法研究进展[J]. 食品工业,2021,42(1):280−284. [SU Wen, YANG Hui, DONG Tengda, et al. Research progress of nanoselenium preparation method[J]. Food Industry,2021,42(1):280−284.] SU Wen, YANG Hui, DONG Tengda, et al. Research progress of nanoselenium preparation method[J]. Food Industry, 2021, 42（1）: 280−284.
[5]	杨颖, 厉舒祯, 范书伶, 等. 贪铜杆菌Cupriavidus sp. SHE细胞上清液合成纳米硒[J]. 生物工程学报,2020,36(6):1162−1169. [YANG Ying, LI Shuzhen, FAN Shuling, et al. Cupriavidus sp. Synthesis of nanoselenium from SHE cell supernatant[J]. Chinese Journal of Biotechnology,2020,36(6):1162−1169.] YANG Ying, LI Shuzhen, FAN Shuling, et al. Cupriavidus sp. Synthesis of nanoselenium from SHE cell supernatant[J]. Chinese Journal of Biotechnology, 2020, 36（6）: 1162−1169.
[6]	鲍唯. 细菌合成纳米硒微颗粒用于肿瘤化动力治疗[D]. 武汉:华中科技大学, 2020. [BAO Wei. Bacterial synthesis of nanoselenium microparticles for kinetic therapy of tumor[D]. Wuhan:Huazhong University of Science and Technology, 2020.] BAO Wei. Bacterial synthesis of nanoselenium microparticles for kinetic therapy of tumor[D]. Wuhan: Huazhong University of Science and Technology, 2020.
[7]	殷婷婷, 李志慧, 苏佳贺, 等. 生物法制备纳米硒的研究进展和应用前景[J]. 中国农学通报,2022,38(8):33−41. [YIN Tingting, LI Zhihui, SU Jiahe, et al. Research progress and application prospect of biological preparation of nanoselenium[J]. Chinese Agricultural Science Bulletin,2022,38(8):33−41.] YIN Tingting, LI Zhihui, SU Jiahe, et al. Research progress and application prospect of biological preparation of nanoselenium[J]. Chinese Agricultural Science Bulletin, 2022, 38（8）: 33−41.
[8]	刘明珠, 魏凌峰, 蔡杰, 等. 多糖-纳米硒在食品包装中应用的研究进展[J]. 食品科技,2022,47(3):1−7. [LIU Mingzhu, WEI Lingfeng, CAI Jie, et al. Research progress on the application of polysaccharide-nanoselenium in food packaging[J]. Food Science and Technology,2022,47(3):1−7.] doi: 10.3969/j.issn.1005-9989.2022.3.spkj202203001 LIU Mingzhu, WEI Lingfeng, CAI Jie, et al. Research progress on the application of polysaccharide-nanoselenium in food packaging[J]. Food Science and Technology, 2022, 47（3）: 1−7. doi: 10.3969/j.issn.1005-9989.2022.3.spkj202203001
[9]	叶园园, 蔡杰, 李楠, 等. 纳米硒在食品领域中的应用研究进展[J]. 食品科技,2020,45(10):11−18. [YE Yuanyuan, CAI Jie, LI Nan, et al. Research progress on the application of nanoselenium in the field of food[J]. Food Science and Technology,2020,45(10):11−18.] YE Yuanyuan, CAI Jie, LI Nan, et al. Research progress on the application of nanoselenium in the field of food[J]. Food Science and Technology, 2020, 45（10）: 11−18.
[10]	刘晓庆, 魏凌峰, 贾继来, 等. 纳米硒多糖载体的构建及其抗肿瘤应用的研究进展[J]. 食品工业科技,2022,43(21):454−460. [LIU Xiaoqing, WEI Lingfeng, JIA Jilai, et al. Research progress on the construction of nanoselenium polysaccharide carriers and their anti-tumor applications[J]. Science and Technology of Food Industry,2022,43(21):454−460.] LIU Xiaoqing, WEI Lingfeng, JIA Jilai, et al. Research progress on the construction of nanoselenium polysaccharide carriers and their anti-tumor applications[J]. Science and Technology of Food Industry, 2022, 43（21）: 454−460.
[11]	王皓, 王玉丽, 孙洁洁, 等. 纳米硒在医药领域中的应用研究进展[J]. 国际药学研究杂志,2020,47(5):337−341,346. [WANG Hao, WANG Yuli, SUN Jiejie, et al. Research progress on the application of nanoselenium in the field of medicine[J]. International Journal of Pharmaceutical Research,2020,47(5):337−341,346.] WANG Hao, WANG Yuli, SUN Jiejie, et al. Research progress on the application of nanoselenium in the field of medicine[J]. International Journal of Pharmaceutical Research, 2020, 47（5）: 337−341,346.
[12]	周驰. 纳米硒的生物合成及其抑菌活性的研究[D]. 合肥:安徽农业大学, 2018. [ZHOU Chi. Biosynthesis of nanoselenium and its bacteriostatic activity[D]. Hefei:Anhui Agricultural University, 2018.] ZHOU Chi. Biosynthesis of nanoselenium and its bacteriostatic activity[D]. Hefei: Anhui Agricultural University, 2018.
[13]	杨锐, 余雍和, 程水源, 等. 耐硒芽孢杆菌的筛选及其亚硒酸盐还原机制的探究[J]. 食品工业科技,2021,42(22):105−111. [YANG Rui, YU Yonghe, CHENG Shuiyuan, et al. Screening of Bacillus selenium-resistant and its reduction mechanism of selenite[J]. Science and Technology of Food Industry,2021,42(22):105−111.] YANG Rui, YU Yonghe, CHENG Shuiyuan, et al. Screening of Bacillus selenium-resistant and its reduction mechanism of selenite[J]. Science and Technology of Food Industry, 2021, 42（22）: 105−111.
[14]	朱燕云, 孔祥平, 吴娥娇, 等. 耐高盐枯草芽孢杆菌XP合成球形纳米硒及其抑制草莓病原真菌生物活性[J]. 生物工程学报,2021,37(8):2825−2835. [ZHU Yanyun, KONG Xiangping, WU Ejiao, et al. Synthesis of spherical nanoselenium byBacillus subtilis XP and its inhibition of biological activity of strawberry pathogenic fungi[J]. Chinese Journal of Biotechnology,2021,37(8):2825−−2835.] ZHU Yanyun, KONG Xiangping, WU Ejiao, et al. Synthesis of spherical nanoselenium by Bacillus subtilis XP and its inhibition of biological activity of strawberry pathogenic fungi[J]. Chinese Journal of Biotechnology, 2021, 37（8）: 2825−−2835.
[15]	利军, 马英辉, 卢美欢. 纳米硒合成细菌Lxz-41的鉴定、培养条件优化及其在富硒猕猴桃栽培中的应用[J]. 食品工业科技,2019,40(21):110−117. [LI Jun, MA Yinghui, LU Meihuan. Identification and optimization of culture conditions of nanoselenium synthetic bacteria lxz-41 and its application in the cultivation of selenium-rich kiwifruit[J]. Science and Technology of Food Industry,2019,40(21):110−117.] LI Jun, MA Yinghui, LU Meihuan. Identification and optimization of culture conditions of nanoselenium synthetic bacteria lxz-41 and its application in the cultivation of selenium-rich kiwifruit[J]. Science and Technology of Food Industry, 2019, 40（21）: 110−117.
[16]	TAN Y Q, YAO R, WANG R, et al. Reduction of selenite to Se(0) nanoparticles by filamentous bacterium Streptomyces sp. ES2-5 isolated from a selenium mining soil[J]. Microbial Cell Factories,2016,15:157. doi: 10.1186/s12934-016-0554-z
[17]	ZHANG L Y, LI Z T, ZHANG L, Lei Z, et al. High-efficiency reducing strain for producing selenium nanoparticles isolated from marine sediment[J]. International Journal of Molecular Sciences,2022,23(19):11953. doi: 10.3390/ijms231911953
[18]	ASHENGROPH M, HOSSEINI S R. A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties[J]. International Microbiology: The Official Journal of the Spanish Society for Microbiology,2020,24:103−114.
[19]	SANDHYA M V S, RAJKUMAR K, BURGULA S. Efficient eco-friendly approach towards bimetallic nanoparticles synthesis and characterization using Exiguobacterium aestuarii by statistical optimization[J]. Green Chemistry Letters and Reviews,2019,12:420−434. doi: 10.1080/17518253.2019.1687762
[20]	Wang T, Zhao H Y, Bi Y G, et al. Preparation and antioxidant activity of selenium nanoparticles decorated by polysaccharides from Sargassum fusiforme[J]. Journal of Food Science,2021,86:977−986. doi: 10.1111/1750-3841.15605
[21]	BAKER S, HARINI B P, RAKSHITH D, et al. Marine microbes:invisible nanofactories[J]. Journal of Pharmacy Research,2013,6:383−388. doi: 10.1016/j.jopr.2013.03.001
[22]	CARROLL A R, COPP B R, DAVIS R A, et al. Marine natural products[J]. Natural Product Reports,2020,37:175−223. doi: 10.1039/C9NP00069K
[23]	冯震, 蒋波, 李芳, 等. 细菌DNA特征序列鉴定法在常见种属中的鉴定水平研究[J]. 药物分析杂志,2019,39(11):1924−1932. [FENG Zhen, JIANG Bo, LI Fang, et al. Identification level of bacterial DNA signature sequence identification method in common species[J]. Journal of Pharmaceutical Analysis,2019,39(11):1924−1932.] FENG Zhen, JIANG Bo, LI Fang, et al. Identification level of bacterial DNA signature sequence identification method in common species[J]. Journal of Pharmaceutical Analysis, 2019, 39（11）: 1924−1932.
[24]	KURODA M, YAMASHITA M, MIWA E, et al. Molecular cloning and characterization of the srdBCA operon, encoding the respiratory selenate reductase complex, from the selenate-reducing bacterium Bacillus selenatarsenatis SF-1[J]. Journal of bacteriology,2011,193(9):2141−2148. doi: 10.1128/JB.01197-10
[25]	YILAMZ M T, ISPIRLI H, TAYLAN O, et al. A green nano-biosynthesis of selenium nanoparticles with Tarragon extract:Structural, thermal, and antimicrobial characterization[J]. LWT-Food Science and Technology,2021,141:110969. doi: 10.1016/j.lwt.2021.110969
[26]	ULLAH A, YIN X Y, WANG F H, et al. Biosynthesis of selenium nanoparticles (via Bacillus subtilis BSN313), and their isolation, characterization, and bioactivities[J]. Molecules,2021,26:5559. doi: 10.3390/molecules26185559
[27]	LI S K, SHEN Y H, XIE A J, et al. Rapid, room-temperature synthesis of amorphous selenium/protein composites using Capsicum annuum L extract[J]. Nanotechnology,2007,18:405101. doi: 10.1088/0957-4484/18/40/405101
[28]	AN C H, TANG K B, LIU X M, et al. Large-scale synthesis of high quality trigonal selenium nanowires[J]. European Journal of Inorganic Chemistry,2003,17:3250−3255.
[29]	SENTHIL K C K, AGILAN S, VELAUTHAPILLAI D, et al. Synthesis and characterization of selenium nanowires[J]. Nanotechnology,2011,18:405101.
[30]	王丽红, 杨辉, 苏文, 等. 植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性[J]. 食品科学,2022,43(2):217−223. [WANG Lihong, YANG Hui, SU Wen, et al. Lactobacillus plantarum LP21 green synthetic nanoselenium and its bacteriostatic activity against Vibrio algaelyticum[J]. Food Science,2022,43(2):217−223.] WANG Lihong, YANG Hui, SU Wen, et al. Lactobacillus plantarum LP21 green synthetic nanoselenium and its bacteriostatic activity against Vibrio algaelyticum[J]. Food Science, 2022, 43（2）: 217−223.
[31]	PENG D G, ZHANG J S, LIU Q L, et al. Size effect of elemental selenium nanoparticles (Nano-Se) at supranutritional levels on selenium accumulation and glutathione S-transferase activity[J]. Journal of Inorganic Biochemistry,2007,101(10):1457−1463. doi: 10.1016/j.jinorgbio.2007.06.021
[32]	TANG L, JIA X E, JIANG X F, et al. In vitro study on the individual and synergistic cytotoxicity of adriamycin and selenium nanoparticles against Bel7402 cells with a quartz crystal microbalance[J]. Biosensors and Bioelectronics,2009,24(7):2268−2272. doi: 10.1016/j.bios.2008.10.030
[33]	YE X G, CHEN Z Z, ZHANG Y Y, et al. Construction, characterization, and bioactive evaluation of nano-selenium stabilized by green tea nano-aggregates[J]. LWT-Food Science and Technology,2020,129:109475. doi: 10.1016/j.lwt.2020.109475
[34]	BAO P, XIAO K Q, WANG H J, et al. Characterization and potential applications of a selenium nanoparticle producing and nitrate reducing bacterium Bacillus oryziterrae sp. nov[J]. Scientific Reports,2016,6:34054. doi: 10.1038/srep34054
[35]	LAMPIS S, ZONARO E, BERTOLINI C, et al. Delayed formation of zero-valent selenium nanoparticles by Bacillus mycoides SeITE01 as a consequence of selenite reduction under aerobic conditions[J]. Microbial Cell Factories 2014, 13:35.
[36]	吴光燕, 陆海燕, 杨娟, 等. 一例大肠杆菌的分离与鉴定[J]. 农业开发与装备,2022,251(11):200−201. [WU Guangyan, LU Haiyan, YANG Juan, et al. Isolation and identification of a case of E. coli[J]. Agricultural Development and Equipment,2022,251(11):200−201.] WU Guangyan, LU Haiyan, YANG Juan, et al. Isolation and identification of a case of E. coli[J]. Agricultural Development and Equipment, 2022, 251（11）: 200−201.
[37]	ANANTH A, KEERTHIKA V, RUBY R M. Synthesis and characterization of nano-selenium and its antibacterial response on some important human pathogens[J]. Current Science,2019,116(2):285−290. doi: 10.18520/cs/v116/i2/285-290
[38]	万圣, 吕磊, 叶承华, 等. 1株非典型李斯特菌的鉴定分析[J]. 中国卫生检验杂志,2023,33(1):48−50,54. [WAN Sheng, LÜ Lei, YE Chenghua, et al. Identification and analysis of 1 strain of Atypical Listeria[J]. Chinese Journal of Health Laboratory and Laboratory,2023,33(1):48−50,54.] WAN Sheng, LÜ Lei, YE Chenghua, et al. Identification and analysis of 1 strain of Atypical Listeria[J]. Chinese Journal of Health Laboratory and Laboratory, 2023, 33（1）: 48−50,54.
[39]	刘欣, 王真, 王园, 等. 持久性单增李斯特菌的抗逆表型及相关功能基因的研究进展[J/OL].食品与发酵工业: 1-9[2023-10-30]. https://doi.org/10.13995/j.cnki.11-1802/ts.034407. [LIU Xin, WANG Zhen, WANG Yuan, et al. Research progress on anti-stress phenotypes and related functional genes of Listeria monocytogenes persistence[J/OL]. Food and Fermentation Industry: 1-9[2023-10-30]. https://doi.org/10.13995/j.cnki.11-1802/ts.034407.] LIU Xin, WANG Zhen, WANG Yuan, et al. Research progress on anti-stress phenotypes and related functional genes of Listeria monocytogenes persistence[J/OL]. Food and Fermentation Industry: 1-9[2023-10-30]. https://doi.org/10.13995/j.cnki.11-1802/ts.034407.