Changes in the Bacterial Phases of Soybean Curd Sheets and Analysis of Spoilage Ability of Dominant Bacteria Stored at Normal Temperature

YAN Yanjun; ZHANG Youhui; GAO Feng; LI Ya; LIU Changjin

doi:10.13386/j.issn1002-0306.2023010088

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 140-149. > DOI: 10.13386/j.issn1002-0306.2023010088

YAN Yanjun, ZHANG Youhui, GAO Feng, et al. Changes in the Bacterial Phases of Soybean Curd Sheets and Analysis of Spoilage Ability of Dominant Bacteria Stored at Normal Temperature[J]. Science and Technology of Food Industry, 2023, 44(19): 140−149. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023010088.

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Changes in the Bacterial Phases of Soybean Curd Sheets and Analysis of Spoilage Ability of Dominant Bacteria Stored at Normal Temperature

1.
College of Food Science & Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
2.
National Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China

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Received Date: January 11, 2023
Available Online: August 04, 2023

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Abstract

Abstract

The bacterial phase changes and the effect of dominant spoilage bacteria on quality were studied with traditional identification method and high-throughput sequence technique using Chinese traditional food soybean curd sheets as materials. Sterile soybean curd sheets were inoculated with three dominant bacteria and their combination. The total viable counts, total volatile base nitrogen (TVB-N), pH, sensory score, and texture characteristics of samples during normal temperature storage were measured periodically for spoilage potential. The scanning electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Fourier transform infrared spectroscopy (FTIR) were used to determine the effect of spoilage bacteria on the protein. The results indicated the bacterial phases of soybean curd sheets changed obviously during storage, and Acinetobacter, Staphylococcus as well as Kurthia became the dominant bacteria after 2 days of storage. The selected representative spoilage bacteria were re-inoculated to the samples, one protease-producing Staphylococcus sciuri and the mixed bacteria had the strongest spoilage potential, because the TVB-N and viscosity increased greatly, while the hardness, springiness, and chewiness decreased significantly (P<0.05). Scanning electron microscopy revealed that the surface protein structure of the samples was distorted and many holes appeared. SDS-PAGE result showed that the band of soybean protein 7S subunit faded or disappeared, however, the bands below 25 kDa were deepened obviously and a new band appeared. FTIR showed that the soybean protein β-sheet structure decreased, while the β-turn structure increased. In conclusion, Staphylococcus sciur is identified as specific spoilage bacteria of soybean curd sheets during normal temperature storage, it provides a valuable reference for the spoilage bacteria control and prolong the product shelf-life.
- soybean curd sheets,
- high-throughput sequencing,
- dominant spoilage bacteria,
- spoilage ability,
- protein structure

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References

[1]	王睿智. 水质对干豆腐品质的影响机制及调控技术[D]. 哈尔滨: 哈尔滨商业大学, 2021 WANG R Z. Effects of water quality on quality of dried bean curd and it's regulatory techniques[D]. Harbin: Harbin University of Commerce, 2021.
[2]	LEE D Y, KWON K H, CHAI C, et al. Microbial contamination of tofu in Korea and growth characteristics of Bacillus cereus isolates in tofu[J]. LWT-Food Science and Technology,2017,78:63−69. doi: 10.1016/j.lwt.2016.11.081
[3]	曲敏, 陈红丽, 王宇, 等. 传统豆制品腐败菌污染及抑制研究进展[J]. 食品科学技术学报,2022,40(3):167−178. [QU M, CHEN H L, WANG Y, et al. Research progress of spoilage bacteria pollution and inhibition of traditional soyproducts[J]. Journal of Food Science and Technology,2022,40(3):167−178. QU M, CHEN H L, WANG Y, et al. Research progress of spoilage bacteria pollution and inhibition of traditional soyproducts[J]. Journal of Food Science and Technology, 2022, 40(3): 167-178.
[4]	LIU J G, LIN T S, LIN W Y. Evaluating the growth of Listeria monocytogenes that has been inoculated into tofu containing background microflora[J]. Food Control,2010,21(12):1764−1768. doi: 10.1016/j.foodcont.2010.09.001
[5]	ROSSI F, FELIS G E, MARTINELLI A, et al. Microbiological characteristics of fresh tofu produced in small industrial scale and identification of specific spoiling microorganisms (SSO)[J]. LWT-Food Science and Technology,2016,70:280−285. doi: 10.1016/j.lwt.2016.02.057
[6]	COCOLIN L, ALESSANDRIA V, DOLCI P, et al. Culture independent methods to assess the diversity and dynamics of microbiota during food fermentation[J]. International Journal of Food Microbiology,2013,167(1):29−43. doi: 10.1016/j.ijfoodmicro.2013.05.008
[7]	杨光萍. 高通量测序技术在食品微生物检测中的应用[J]. 食品安全导刊,2022,359(30):182−185. [YANG G P. Application of high-throughput sequencing technology in food microbial detection[J]. Food Safety Guide,2022,359(30):182−185. YANG G P. Application of high-throughput sequencing technology in food microbial detection[J]. Food Safety Guide, 2022, 359(30): 182-185.
[8]	江杨阳, 杨水兵, 余海霞, 等. 基于培养基法和高通量测序法分析冷藏小龙虾优势腐败菌[J]. 食品科学,2019,40(16):130−136. [JIANG Y Y, YANG S B, YU H X, et al. Analysis of specific spoilage organisms in chilled red swamp crayfish using culture-dependent method and high-throughput sequencing[J]. Food Science,2019,40(16):130−136. doi: 10.7506/spkx1002-6630-20180718-229 JIANG Y Y, YANG S B, YU H X, et al. Analysis of specific spoilage organisms in chilled red swamp crayfish using culture-dependent method and high-throughput sequencing[J]. Food Science, 2019, 40(16): 130-136. doi: 10.7506/spkx1002-6630-20180718-229
[9]	HUANG Z, LIU X C, JIA S L, et al. Antimicrobial effects of cinnamon bark oil on microbial composition and quality of grass carp (Ctenopharyngodon idellus) fillets during chilled storage[J]. Food Control,2017,82:316−324. doi: 10.1016/j.foodcont.2017.07.017
[10]	GRAM L, RAVN L, RASCH M, et al. Food spoilage-interactions between food spoilage bacteria[J]. International Journal of Food Microbiology,2002,78(1−2):79−97. doi: 10.1016/S0168-1605(02)00233-7
[11]	冯豪杰, 蓝蔚青, 臧一宇, 等. 优势腐败菌对暗纹东方鲀冷藏期间品质变化影响及致腐能力分析[J]. 食品科学,2022,43(1):1191−1197. [FENG H J, LAN W Q, ZANG Y Y, et al. Effects of dominant spoilage bacteria on quality change in obscure pufferfish (Takifugu obscurus) during cold storage and analysis of their spoilage ability[J]. Food Science,2022,43(1):1191−1197. FENG H J, LAN W Q, ZANG Y Y, et al. Effects of dominant spoilage bacteria on quality change in obscure pufferfish (Takifugu obscurus) during cold storage and analysis of their spoilage ability[J]. Food Science, 2022, 43(1): 1191-1197.
[12]	郭全友, 单珂, 姜朝军, 等. 源自轻腌大黄鱼的特定腐败菌特性及腐败能力[J]. 食品科学,2020,41(1):24−32. [GUO Q Y, SHAN K, JIANG C J, et al. Characterization and spoilage potential of specific spoilage organisms isolated from lightly salted large yellow croaker (Pseudosciaenac rocea)[J]. Food Science,2020,41(1):24−32. GUO Q Y, SHAN K, JIANG C J, et al. Characterization and spoilage potential of specific spoilage organisms isolated from lightly salted large yellow croaker (Pseudosciaenac rocea)[J]. Food Science, 2020, 41(1): 24-32.
[13]	JIA S L, LI Y, ZHUANG S, et al. Biochemical changes induced by dominant bacteria in chill-stored silver carp (Hypophthalmichthys molitrix) and GC-IMS identification of volatile organic compounds[J]. Food Microbiology,2019,84:103248. doi: 10.1016/j.fm.2019.103248
[14]	于淑池, 杨毅, 冯紫蓝, 等. 冷藏卵形鲳优势腐败菌的分离鉴定及致腐能力分析[J]. 食品工业科技,2021,42(1):101−109. [YU S C, YANG Y, FENG Z L, et al. Isolation and identification of dominant spoilage bacteria in Trachinotus ovatus during chilled storage and their spoilage capability[J]. Science and Technology of Food Industry,2021,42(1):101−109. YU S C, YANG Y, FENG Z L, et al. Isolation and identification of dominant spoilage bacteria in Trachinotus ovatus during chilled storage and their spoilage capability[J]. Science and Technology of Food Industry, 2021, 42(1): 101-109.
[15]	韩翠萍, 葛子榜, 刘庆冠, 等. 豆腐中主要腐败菌的分离鉴定及与品质的相关性分析[J]. 中国食品学报,2019,19(7):283−291. [HAN C P, GE Z B, LIU G Q, et al. Isolation and identification of main spoilage bacteria in tofu and its correlation with quality[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(7):283−291. HAN C P, GE Z B, LIU G Q, et al. Isolation and identification of main spoilage bacteria in tofu and its correlation with quality[J]. Journal of Chinese Institute of Food Science and Technology, 2019, 19(7): 283-291.
[16]	杨立娜, 赵亚凡, 马丹丹, 等. Nisin及纳他霉素生物保鲜剂对干豆腐保鲜效果的研究[J]. 食品研究与开发,2020,41(10):21−27. [YANG L N, ZHAO Y F, MA D D, et al. Effect of nisin and natamycin biological preservative on fresh-keeping of dried tofu[J]. Food Research and Development,2020,41(10):21−27. YANG L N, ZHAO Y F, MA D D, et al. Effect of nisin and natamycin biological preservative on fresh-keeping of dried tofu[J]. Food Research and Development, 2020, 41(10): 21-27.
[17]	郑卫, 田海娟, 张传智. 壳聚糖溶液对豆腐保质期的影响[J]. 粮食与油脂,2020,33(5):56−58. [ZHENG W, TIAN H J, ZHANG C Z. Research on the quality of tofu with chitosan solution[J]. Grain and Oil,2020,33(5):56−58. ZHENG W, TIAN H J, ZHANG C Z. Research on the quality of tofu with chitosan solution[J]. Grain and Oil, 2020, 33(5): 56-58.
[18]	张利, 卢利平, 丁功涛, 等. 一株产蛋白酶粘质沙雷氏菌的分子鉴定与发酵条件优化[J]. 农产品加工,2021(13):64−69. [ZHANG L, LU L P, DING G T, et al. Molecular identification and fermentation conditions optimization of a protease producing serratia marcescens[J]. Farm Products Processing,2021(13):64−69. doi: 10.16693/j.cnki.1671-9646(X).2021.07.014 ZHANG L, LU L P, DING G T, et al. Molecular identification and fermentation conditions optimization of a protease producing serratia marcescens[J]. Farm Products Processing, 2021(13): 64-69. doi: 10.16693/j.cnki.1671-9646(X).2021.07.014
[19]	LI Y, HUAG J Q, YUAN C H, et al. Developing a new spoilage potential algorithm and identifying spoilage volatiles in small yellow croaker (Larimichthys polyactis) under vacuum packaging condition[J]. LWT-Food Science and Technology,2019,106:209−217. doi: 10.1016/j.lwt.2019.02.075
[20]	中华人民共和国国家卫生和计划委员会, 国家食品药品监督管理总局. GB 4789.2-2016 食品安全国家标准食品微生物学检验菌落总数测定[S]. 北京: 中国标准出版社, 2016 The National Health and Planning Commission of the People's Republic of China, the State Food and Drug Administration. GB 4789.2-2016 National food safety standards, food microbiological inspection, aerobic plate count[S]. Beijing: China Standards Press, 2016.
[21]	中华人民共和国国家卫生和计划委员会. GB 5009.228-2016 食品安全国家标准食品中挥发性盐基氮的测定[S]. 北京: 中国标准出版社, 2016 The National Health and Planning Commission of the People's Republic of China. GB 5009.228-2016 National food safety standards, determination of total volatile basic nitrogen in food[S]. Beijing: China Standards Press, 2016.
[22]	赖晗. 大豆品种和两种凝固剂对豆腐品质的影响[D]. 南昌: 南昌大学, 2019 LAI H. Effects of soybean varieties and two coagulants on the quality of tofu[D]. Nanchang: Nanchang University, 2019.
[23]	XIONG T, LI J B, LIANG F, et al. Effects of salt concentration on Chinese sauerkraut fermentation[J]. LWT-Food Science and Technology,2016,69:169−174. doi: 10.1016/j.lwt.2015.12.057
[24]	ZHU S Q, WU H H, ZENG M Y, et al. The involvement of bacterial quorum sensing in the spoilage of refrigerated Litopenaeus vannamei[J]. International Journal of Food Microbiology,2015,192:26−33. doi: 10.1016/j.ijfoodmicro.2014.09.029
[25]	SHAO L T, TIAN Y, CHEN S S, et al. Characterization of the spoilage heterogeneity of Aeromonas isolated from chilled chicken meat: In vitro and in situ[J]. LWT-Food Science and Technology,2022,162:113470. doi: 10.1016/j.lwt.2022.113470
[26]	NOWAK A, PIOTROWSKA M. Biochemical activities of Brochothrix thermosphacta[J]. Meat Science,2012,90(2):410−413. doi: 10.1016/j.meatsci.2011.08.008
[27]	SANCHEZ M M, STAVROPOULOU D A, LEROY F. Exploring the metabolic heterogeneity of coagulase-negative Staphylococci to improve the quality and safety of fermented meats: A review[J]. International Journal of Food Microbiology,2017,247:24−37. doi: 10.1016/j.ijfoodmicro.2016.05.021
[28]	ZHAO L J, JIA L R, MA B C, et al. Heat-resistant bacteria contamination investigation in Chinese soybean curd industrial processing using high-throughput gene sequencing and MALDI-TOF-MS[J]. LWT-Food Science and Technology,2021,147:111618. doi: 10.1016/j.lwt.2021.111618
[29]	李博, 籍保平. 葡萄糖酸内酯豆腐生产过程中微生物的变化及豆腐中主要腐败菌的鉴定[J]. 食品科学,2006(5):77−82. [LI B, JI B P. Isolation and identification of major purified bacteria in glucono-delta-lactone (GDL) tofu processing[J]. Food Science,2006(5):77−82. doi: 10.3321/j.issn:1002-6630.2006.05.013 LI B, JI B P. Isolation and identification of major purified bacteria in glucono-delta-lactone (GDL) tofu processing[J]. Food Science, 2006(5): 77-82. doi: 10.3321/j.issn:1002-6630.2006.05.013
[30]	CARVALHEIRA A, FERREIRA V, SILVA J, et al. Enrichment of Acinetobacter spp. from food samples[J]. Food Microbiol,2016,55:123−127. doi: 10.1016/j.fm.2015.11.002
[31]	HAMOUDA A, FINDLAY J, AL HASSAN L, et al. Epidemiology of Acinetobacter baumannii of animal origin[J]. International Journal of Antimicrobial Agents,2011,38(4):314−318. doi: 10.1016/j.ijantimicag.2011.06.007
[32]	SOMMERS C, SHEEN S, SCULLEN O J, et al. Inactivation of Staphylococcus saprophyticus in chicken meat and purge using thermal processing, high pressure processing, gamma radiation, and ultraviolet light (254 nm)[J]. Food Control,2017,75:78−82. doi: 10.1016/j.foodcont.2016.12.020
[33]	MACE S, JOFFRAUD J J, CARDINAL M, et al. Evaluation of the spoilage potential of bacteria isolated from spoiled raw salmon (Salmo salar) fillets stored under modified atmosphere packaging[J]. International Journal of Food Microbiology,2013,160(3):227−238. doi: 10.1016/j.ijfoodmicro.2012.10.013
[34]	MOUSAKHANI-GANJEH A, HAMDAMI N, SOLTANIZADEH N. Impact of high voltage electric field thawing on the quality of frozen tuna fish (Thunnus albacares)[J]. Journal of Food Engineering,2015,156:39−44. doi: 10.1016/j.jfoodeng.2015.02.004
[35]	MACE S, CARDINAL M, JAFFRES E, et al. Evaluation of the spoilage potential of bacteria isolated from spoiled cooked whole tropical shrimp (Penaeus vannamei) stored under modified atmosphere packaging[J]. Food Microbiol,2014,40:9−17. doi: 10.1016/j.fm.2013.11.018
[36]	刘舒彦, 熊光权, 李海蓝, 等. 加州鲈优势腐败菌的分离鉴定[J]. 现代食品科技,2019,35(11):120−125. [LIU S Y, XIONG G Q, LI H L, et al. Isolation and identification of the dominant spoilage bacteria from california bass (Micropterus salmoides)[J]. Modern Food Science and Technology,2019,35(11):120−125. doi: 10.13982/j.mfst.1673-9078.2019.11.017 LIU S Y, XIONG G Q, LI H L, et al. Isolation and identification of the dominant spoilage bacteria from california bass (Micropterus salmoides)[J]. Modern Food Science and Technology, 2019, 35(11): 120-125. doi: 10.13982/j.mfst.1673-9078.2019.11.017
[37]	LIU J L, LAN W Q, SUN X H, et al. Effects of chitosan grafted phenolic acid coating on microbiological, physicochemical and protein changes of sea bass (Lateolabrax japonicus) during refrigerated storage[J]. Journal of Food Science,2020,85(8):2506−2515. doi: 10.1111/1750-3841.15329
[38]	DELBARRE-LADRAT C, CHÉRET R, TAYLOR R, et al. Trends in postmortem aging in fish: Understanding of proteolysis and disorganization of the myofibrillar structure[J]. Critical Reviews in Food Science and Nutrition,2006,46(5):409−421. doi: 10.1080/10408390591000929
[39]	SHEN G H, ZHENG L J, LI S S, et al. The role of soy protein degradation caused by spoilage Bacillus amyloliquefaciens in texture deterioration of yuba, a soy product[J]. LWT-Food Science and Technology,2020,123:108109.
[40]	ZHUANG S, LIU Y Y, GAO S, et al. Mechanisms of fish protein degradation caused by grass carp spoilage bacteria: A bottom-up exploration from the molecular level, muscle microstructure level, to related quality changes[J]. Food Chemistry,2022,403:134309.
[41]	HUANG L, XU Y, ZHOU Y M. Improvement of nutritional quality of soybean meal by Fe(II)-assisted acetic acid treatment[J]. Food Chemistry,2019,283:475−480. doi: 10.1016/j.foodchem.2019.01.085
[42]	PISACANE V, CALLEGARI M L, PUGLISI E, et al. Microbial analyses of traditional Italian salami reveal microorganisms transfer from the natural casing to the meat matrix[J]. International Journal of Food Microbiology,2015,207:57−65. doi: 10.1016/j.ijfoodmicro.2015.04.029
[43]	WANG C, JIANG L Z, WEI D X, et al. Effect of secondary structure determined by FTIR spectra on surface hydrophobicity of soybean protein isolate[J]. Science and Technology of Food Industry,2011,15(1):4819−4827.
[44]	ZHAO X, ZHOU Y G, ZHAO L, et al. Vacuum impregnation of fish gelatin combined with grape seed extract inhibits protein oxidation and degradation of chilled tilapia fillets[J]. Food Chemistry,2019,294:316−325. doi: 10.1016/j.foodchem.2019.05.054