Effect of Yujing Jiangtang Recipe on Blood Glucose, Lipid and Serum Oxidative Stress Related Indexes in Type 2 diabetes Rats

ZHOU Xiran; JIAO Gena; ZENG Ji; LI Bohan; SHEN Lu

doi:10.13386/j.issn1002-0306.2022120224

Volume 44 Issue 19

Oct. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(19): 433-439. > DOI: 10.13386/j.issn1002-0306.2022120224

ZHOU Xiran, JIAO Gena, ZENG Ji, et al. Effect of Yujing Jiangtang Recipe on Blood Glucose, Lipid and Serum Oxidative Stress Related Indexes in Type 2 diabetes Rats[J]. Science and Technology of Food Industry, 2023, 44(19): 433−439. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120224.

Citation:

PDF (2786 KB)

Effect of Yujing Jiangtang Recipe on Blood Glucose, Lipid and Serum Oxidative Stress Related Indexes in Type 2 diabetes Rats

1.
Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi’an 710003, China
2.
Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China

More Information

Received Date: December 28, 2022
Available Online: August 04, 2023

Graphical Abstract

Abstract

Abstract

Objective: To study the effect of Yujing Jiangtang Recipe on blood glucose, lipid and antioxidation in type 2 diabetes rats. Methods: High fat diet and intraperitoneal injection of 40 mg/kg STZ were used to establish the model of type 2 diabetes rats. The successful rats were randomly divided into diabetes model group, metformin group (0.2 g/kg), low, medium and high dose Yujing Jiangtang Recipe groups (1、2、4 g/kg), and another normal control group with nine rats in each group. The treatment group was gavaged with corresponding dose, the normal control group and the diabetes model group were gavaged with 10 mL/kg normal saline according to weight, and each group was gavaged once a day for six consecutive weeks. During the experiment, observe and record the weight changes of rats in each group, and measure the fasting blood glucose (FBG). After continuous gavage for six weeks, the abdominal aorta blood of rats was taken, the fasting insulin (FINS) level and the percentage content of glycosylated hemoglobin (HbA1c) were measured, and the HOMA-IR, ISI were calculated. The contents of serum TG, TC, HDL-C, LDL-C were measured. The serum levels of MDA, SOD, GSH, NO, GSH-Px and CAT were measured by ELISA. The pancreatic tissue of rats was stained with HE to observe the pathological changes of rats. Results: Compared with the diabetes model group, the body weight of the high dose group of Yujing Jiangtang Recipe increased significantly (P<0.01). Each dose group of Yujing Jiangtang Recipe could significantly reduce FBG (P<0.01). It could reduce the levels of FINS, HOMA-IR and the percentage of HbA1c in rats to varying degrees, and increase the level of ISI (P<0.05 or P<0.01). It could reduce the content of serum TG, TC, LDL-C and MDA (P<0.05 or P<0.01), and increase the content of HDL-C and the level of SOD and GSH (P<0.01). The levels of NO, GSH-Px and CAT were significantly increased in the middle and high dose groups of Yujing Jiangtang Recipe (P<0.01). In the high-dose group of Yujing Jiangtang Recipe, the structure of exocrine gland acini was relatively complete and regular, the shape of islet cells was relatively regular, and the number of islet cells was large and evenly arranged. Conclusion: Yujing Jiangtang Recipe has hypoglycemic effect on type 2 diabetes rats, and its mechanism may be related to regulating blood lipid metabolism, improving insulin resistance, antioxidation and repairing pancreatic tissue.
- Yujing Jiangtang recipe,
- type 2 diabetes,
- blood sugar,
- blood lipid,
- oxidative stress

FullText(HTML)

References (31)

References

[1]	American Diabetes Association. Diagnosis and classification of diabetes mellitus[J]. Diabetes Care,2010,3(Suppl 1):S62−S69.
[2]	BROWNLEE M. Biochemistry and molecular cell biology of diabetic complications[J]. Nature,2001,414(6865):813−820. doi: 10.1038/414813a
[3]	CAVANAGH E M, INSERRA F, TOBLLI J, et al. Enalapril attenuates oxidative stress in diabetic rats[J]. Hypertension,2001,38(5):1130−1136. doi: 10.1161/hy1101.092845
[4]	GIACCO F, BROWNLEE M. Oxidative stress and diabetic complications[J]. Circ Res,2010,107(9):1058−1070. doi: 10.1161/CIRCRESAHA.110.223545
[5]	SHARMA M, GUPTA S, SINGH K, et al. Association of glutathione-S-transferase with patients of type 2 diabetes mellitus with and without nephropathy[J]. Diabetes & Metabolic Syndrome:Clinical Research & Reviews,2016,10(4):194−197.
[6]	NEELOFAR K, ARIF Z, ARAFAT M Y, et al. A study on correlation between oxidative stress parameters and inflammatory markers in type 2 diabetic patients with kidney dysfunction in north Indian population[J]. Journal of Cellular Biochemistry,2019,120(4):4892−4902. doi: 10.1002/jcb.27763
[7]	ABDULMALEK S A, BALBAA M. Synergistic effect of nano-selenium and metformin on type 2 diabetic rat model: Diabetic complications alleviation through insulin sensitivity, oxidative mediators and inflammatory markers[J]. PLoS One,2019,14(8):e0220779. doi: 10.1371/journal.pone.0220779
[8]	ALSHARIDAH M, ALGEFFARI M, ABDEL-MONEIM A H, et al. Effect of combined gliclazide/metformin treatment on oxidative stress, lipid profile, and hepatorenal functions in type 2 diabetic patients[J]. Saudi Pharmaceutical Journal,2018,26(1):1−6. doi: 10.1016/j.jsps.2017.11.007
[9]	李佳欣, 陈思琦, 葛鹏玲. 山茱萸药理学研究进展[J]. 辽宁中医药大学学报,2022,24(4):63−66. [LI J X, CHEN S Q, GE P L. Progress in pharmacological studies of Shanzhuyu (cornifructus)[J]. Journal of Liaoning University of Tcm,2022,24(4):63−66. LI J X, CHEN S Q, GE P L. Progress in Pharmacological Studies of Shanzhuyu(Cornifructus)[J]. Journal of Liaoning University of Tcm. 2022, 24(4): 63-66.
[10]	李玥彤, 隋怡, 郝晓伟, 等. 山茱萸叶多糖的理化性质和抗氧化活性[J]. 西北农林科技大学学报(自然科学版),2019,47(8):109−116,143. [LI Y T, SUI Y, HAO X W, et al. Physicochemical properties and antioxidant activities of polysaccharide from Fructus corni leaves[J]. Journal of Northwest A&F University (Nat Sci Ed),2019,47(8):109−116,143. [LI Y T, SUI Y, HAO X W, et al. Physicochemical properties and antioxidant activities of polysaccharide from Fructus corni leaves[J]. Journal of Northwest A&-F University(Nat. Sci. Ed. ), 2019, 47(8): 109-116, 143.
[11]	温媛媛, 王雷, 王硕, 等. 中药山茱萸中熊果酸的体内、体外抗氧化活性及养生保健价值研究[J]. 世界最新医学信息文摘,2018,18(88):328−331. [WEN Y Y, WANG L, WANG S, et al. Study on the anti-oxidant activity of ursolic acid from Cornus offificinalis[J]. World Latest Medicine Information (Electronic Version),2018,18(88):328−331. [WEN Y Y, WANG L, WANG S, FANG F L, et al. Study on the Anti-oxidant Activity of Ursolic Acid from Cornus Offificinalis[J]. World Latest Medicine Information (Electronic Version), 2018, 18(88): 328-331.
[12]	涂明锋, 叶文峰. 黄精的药理作用及临床应用研究进展[J]. 宜春学院学报,2018,40(9):27−31. [TU M F, YE W F. Research progress of pharmacological effects and clinical application of Polygonatum sibiricum[J]. Journal of Yichun University,2018,40(9):27−31. Research Progress of Pharmacological Effects and Clinical Application of Polygonatum sibiricum[J]. Journal of Yichun University, 2018, 40(9): 27-31.
[13]	周忠光, 宫铭海, 王晓慧, 等. 黄精多糖药理作用及机制研究进展[J]. 辽宁中医药大学学报,2021,23(12):1−4. [ZHOU Z G, GONG M H, WANG X H, et al. Research progress on pharmacological action and mechanism of Polygonatum sibiricum polysaccharide[J]. Journal of Liaoning University of Tcm,2021,23(12):1−4. ZHOU Z G, GONG M H, WANG X H, et al. Research Progress on Pharmacological Action and Mechanism of Polygonatum Sibiricum Polysaccharide[J]. Journal of Liaoning University of Tcm, 2021, 23(12): 1-4.
[14]	王迪, 赵健, 周天, 等. 黄精多糖药理作用机制研究进展[J]. 国际中医中药杂志,2023,45(1):122−125. [WANG D, ZHAO J, ZHOU T, et al. Research progress on pharmacological mechanism of polygona-polysaccharose[J]. International Journal of Traditional Chinese Medicine,2023,45(1):122−125. WANG D, ZHAO J, ZHOU T, et al. Research progress on pharmacological mechanism of polygona-polysaccharose[J]. International Journal of Traditional Chinese Medicine, 2023, 45(1): 122-125.
[15]	金海燕, 许建秦, 沈璐, 等. 食药材降糖方治疗糖耐量异常及2型糖尿病气阴两虚证的临床效果[J]. 临床医学研究与实践,2021,6(7):107−109,115. [JIN H Y, XU J Q, SHEN L, et al. Clinical effect of Shiyaocai Jiangtang decoction in the treatment of impaired glucose tolerance and type 2 diabetes mellitus with Qi and Yin deficiency syndrome[J]. Clinical Medical Research and Practice,2021,6(7):107−109,115. JIN H Y, XU J Q, SHEN L, et al. Clinical effect of Shiyaocai Jiangtang decoction in the treatment of impaired glucose tolerance and type 2 diabetes mellitus with Qi and Yin deficiency syndrome[J]. Clinical medical research and practice, 2021, 6(7): 107-109, 115.
[16]	CHEN M, XIAO D, LIU W, et al. Intake of Ganoderma lucidum polysaccharides reverses the disturbed gut microbiota and metabolism in type 2 diabetic rats[J]. International Journal of Biological Macromolecules,2020, Jul 15,155:890−902.
[17]	MINAZ N, RAZDAN R, PATHAK L. Repositioning of molsidomine for its efficacy in diabetes induced erectile dysfunction in rats: In silico, in vitro and in vivo approach[J]. Pharmacological Reports,2018,Apr,70(2):309−315.
[18]	王钜, 陈振文. 现代医学实验动物学概论[M]. 北京: 中国协和医科大学出版社, 2004 WANG J, CHEN Z W. Introduction to modern medical laboratory animal science[M]. Beijing: Peking Union Medical College Press, 2004.
[19]	YAN S, SHI R, LI L, et al. Mannan oligosaccharide suppresses lipid accumulation and appetite in western-diet-induced obese mice via reshaping gut microbiome and enhancing short-chain fatty acids production[J]. Molecular Nutrtion Food Research,2019,63(63):e1900521.
[20]	孙鑫, 马志, 孟庆海, 等. 桑酮碱对2型糖尿病db/db小鼠血糖及肝脏胰岛素抵抗的影响[J]. 中成药,2017,39(5):885−890. [SUN X, MA Z, MENG Q H, et al. Effects of Sangtong alkaloids on blood glucose and hepatic insulin resistance in db/db mice with type 2 diabetes mellitus[J]. Chinese Traditional Patent Medicine,2017,39(5):885−890. doi: 10.3969/j.issn.1001-1528.2017.05.001 [SUN X, MA Z, MENG Q H, et al. Effects of Sangtong alkaloids on blood glucose and hepatic insulin resistance in db/db mice with type 2 diabetes mellitus[J]. Chinese Traditional Patent Medicine, 2017, 39(5): 885-890. doi: 10.3969/j.issn.1001-1528.2017.05.001
[21]	翁丽丽, 陈丽, 宿莹, 等. 知母化学成分和药理作用[J]. 吉林中医药,2018,38(1):90−92. [WENG L L, CHEN L, XU Y, et al. Chemical compositionand pharmacological action of Rhizoma Anemarrhenae[J]. Jilin Journal of Chinese Medicine,2018,38(1):90−92. WENG L L, CHEN L, XU Y, et al. Chemical compositionand pharmacological action of Rhizoma Anemarrhenae[J]. Jilin Journal of Chinese Medicine, 2018, 38(1): 90-92.
[22]	王晓芹, 邓小燕, 于晓斌, 等. 茶多酚通过降脂、抗炎、抗氧化以及调控TGF-β/Smad信号通路缓解2型糖尿病[J]. 中药药理与临床,2018,34(3):46−50. [WANG X Q, DENG X Y, YU X B, et al. Green tea polyphenols ameliorates Type 2 diabetes mellitus through lipid-lowering, anti-oxidation, anti-inflammation and regulating TGF-β/Smad signal pathway[J]. Pharmacology and Clinics of Chinese Materia Medica,2018,34(3):46−50. Wang X Q, Deng X Y, Yu X B, et al. Green tea polyphenols ameliorates Type 2 diabetes mellitus through lipid-lowering, anti-oxidation, anti-inflammation and regulating TGF-β/Smad signal pathway[J]. Pharmacology and Clinics of Chinese Materia Medica, 2018, 34(3): 46-50.
[23]	李吉萍, 袁野, 张文友. 西洋参茎叶皂苷对糖尿病大鼠氧化损伤和血管内皮功能的影响[J]. 中国药理学通报,2017,33(12):1698−1702. [LI J P, YUAN Y, ZHANG W Y. Influence of Panax quinquefolium saponins on oxidative damage and function of vascular endothelium in diabetic rats[J]. Chinese Pharmacological Bulletin,2017,33(12):1698−1702. LI J P, YUAN Y, ZHANG W Y. Influence of Panax quinquefolium saponins on oxidative damage and function of vascular endothelium in diabetic rats[J]. Chinese Pharmacological Bulletin, 2017, 33(12): 1698-1702.
[24]	贾璐, 石洁, 段志倩, 等. 黄精多糖对高脂饲料诱发糖尿病小鼠糖代谢功能的影响[J]. 中国医药导报,2017,14(8):24−28. [JIA L, SHI J, DUAN Z Q, et al. The influence of polygonatum polysaccharide on function of glucose metabolism of high-fat-diet-fed diabetic mice[J]. China Medical Herald,2017,14(8):24−28. [JIA L, SHI J, DUAN Z Q, et al. The influence of polygonatum polysaccharide on function of glucose metabolism of high-fat-diet-fed diabetic mice[J]. China Medical Herald, 2017, 14(8): 24-28.
[25]	赵文莉, 赵晔, YIIDER Tseng. 黄精药理作用研究进展[J]. 中草药,2018,49(18):4439−4445. [ZHAO W, ZHAO Y, YIIDER T. Research progress on pharmacological effects of Polygonati rhizoma[J]. Chinese Traditional and Herbal Drugs,2018,49(18):4439−4445. [ZHAO W, ZHAO Y, YIIDER T. Research progress on pharmacological effects of Polygonati rhizoma[J]. Chinese Traditional and Herbal Drugs, 2018, 49(18): 4439-4445.
[26]	金海燕, 沈璐, 同生, 等. 茶叶及其活性成分降糖作用的研究进展[J]. 陕西农业科学,2020,66(8):81−84. [JIN H Y, SHEN L, TONG S, et al. Research progress on hypoglycemic effect of tea and its active components[J]. Shaanxi Journal of Agricultural Sciences,2020,66(8):81−84. JIN H Y, SHEN L, TONG S, et al. Research progress on hypoglycemic effect of tea and its active components[J]. Shaanxi Journal of Agricultural Sciences, 2020, 66(8): 81-84.
[27]	向臣希, 欧瑜. 氧化应激在2型糖尿病发病过程中的作用[J]. 药物生物技术,2015,22(5):457−460. [XIANG C X, OU Y. Oxidative stress in the development of type 2 diabetes[J]. Pharmaceutical Biotechnology,2015,22(5):457−460. XIANG C X, OU Y. Oxidative Stress in The Development of Type 2 Diabetes[J]. Pharmaceutical Biotechnology, 2015, 22(5): 457-460.
[28]	任春久, 张瑶, 崔为正, 等. 氧化应激在2型糖尿病发病机制中的作用研究进展[J]. 生理学报,2013,65(6):664−673. [REN C J, ZHANG Y, CUI W Z, et al. Progress in the role of oxidative stress in the pathogenesis of type 2 diabetes[J]. Acta Physiologica Sinica,2013,65(6):664−673. REN C J, ZHANG Y, CUI W Z, et al. Progress in the role of oxidative stress in the pathogenesis of type 2 diabetes[J]. Acta Physiologica Sinica, 2013, 65(6): 664-673.
[29]	崔永霞, 王利丽, 孙孝亚, 等. 山茱萸环烯醚萜苷类成分的研究进展[J]. 中华中医药杂志,2021,36(5):2869−2874. [CUI Y X, WANG L L, SUN X Y, et al. Research progress of cornel iridoid glycosides[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2021,36(5):2869−2874. CUI Y X, WANG L L, SUN X Y, et al. Research progress of cornel iridoid glycosides[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2021, 36(5): 2869-2874.
[30]	吴倩, 陈文明, 陈柱梁, 等. 黄精干预糖尿病及其并发症的研究进展[J]. 中医药导报,2022,28(4):180−185. [WU Q, CHEN W M, CHEN Z L, et al. Advance on Huangjing (Polygonati Rhizoma) intervening diabetes and its complications[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy,2022,28(4):180−185. WU Q, CHEN W M, CHEN Z L, et al. Advance on Huangjing (Polygonati Rhizoma) intervening diabetes and its complications[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy,2022, 28(4):180−185.
[31]	苏保洲. 枸杞子活性成分药理作用研究进展[J]. 江苏中医药,2022,54(3):78−81. [SU B Z. Research progress on pharmacological effects of active components of Lycium barbarum L J]. Jiangsu Journal of Traditional Chinese Medicine,2022,54(3):78−81.

Supplements (0)

Cited By

Cited by

Periodical cited type(13)

1.	孙永进，曾惠梅，黄静，赵云龙，蔡锦源，孙松. 吴茱萸多糖的分离纯化、结构表征及体外降脂活性. 食品工业科技. 2025(08): 33-42 . 本站查看
2.	王磊，李国龙，唐志书，宋忠兴，袁书会，刘红波，史鑫波，陈佳昕. 不同生长时期酸枣果肉多糖相对分子质量分布和单糖组成及抗氧化活性研究. 食品工业科技. 2024(07): 1-7 . 本站查看
3.	夏谍，李铭，丁俞珍，邓萌玥，位盼盼，晏子俊，张磊，陈彤. 不同脱色方法对三七多糖物理性质及体外抗氧化活性的影响. 化学研究与应用. 2024(03): 509-521 .
4.	李瑶，熊彩明，张佳乐，冯学珍，冯书珍. 磷酸化裙带菜多糖的制备及结构表征和生物活性分析. 食品科学. 2024(07): 35-42 .
5.	郭雅娟，范军刚，李建珍. 不同干燥方式对骏枣中香气成分的影响. 中国调味品. 2024(04): 173-177 .
6.	刘春阳，白金波，杨尚青，史进阳，秦亚敏，吴德玲，解松子. 枳椇子多糖的酸提取工艺优化及其理化性质与抗氧化活性研究. 食品与发酵工业. 2024(09): 148-156 .
7.	魏炳琦，高小雨，刘延鑫，王义翠. 红枣多糖的结构、生物学活性及产品开发进展. 食品工业科技. 2024(12): 1-9 . 本站查看
8.	董小强，文畅，许金丹，史乐雪，胡玉龙，李杰明，董春红，丁侃. 鼠李科植物枣多糖的活性机制及其结构特征研究进展. 中国药科大学学报. 2024(04): 443-453 .
9.	吴喆，朱佳敏，刘军，符小玉，娄磊，涂亦娴，秦新政，艾合买提江·艾海提. 红枣主要活性成分及其功能活性研究进展. 现代食品科技. 2024(09): 359-369 .
10.	张雅施，李文文，宗爱珍，左兆河，郑振佳，张斌. 菊芋多糖锌的制备及其抗氧化活性评价. 食品工业科技. 2023(12): 251-259 . 本站查看
11.	刘迎欣，伊娟娟，邵怡雯，崔燕，李雪，王金柱，郝利民，鲁吉珂. 布拉氏酵母发酵山药多糖的分离鉴定与体外生物活性探究. 食品工业科技. 2023(14): 154-162 . 本站查看
12.	石训，石勇，孙晓瑞. 基于文献计量的枣多糖研究趋势分析. 现代食品. 2023(21): 55-58 .
13.	代琪，白苑丁，叶俏波，杨小艳，赵小勤，王欣. 不同产地大枣化学成分及其药理作用研究进展. 中国药物评价. 2023(06): 506-511 .