Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

NIE Xuemei; XIE Yun; XU Bozhou; XU Xiuli

doi:10.13386/j.issn1002-0306.2022120095

Volume 44 Issue 23

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(23): 246-252. > DOI: 10.13386/j.issn1002-0306.2022120095

NIE Xuemei, XIE Yun, XU Bozhou, et al. Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap[J]. Science and Technology of Food Industry, 2023, 44(23): 246−252. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120095.

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Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

NIE Xuemei^{1, 2,},
XIE Yun^{1, 2},
XU Bozhou^{1, 2},
XU Xiuli^{1, 2, ,}

1.
Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China
2.
Key Laboratory of Food Quality and Safety for State Market Regulation, Beijing 100176, China

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Received Date: December 12, 2022
Available Online: October 03, 2023

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Abstract

Objective: To prevent some illegal companies driven by interests from arbitrarily labeling "organic" to deceive consumers using ways such as counterfeiting, forgery, and shoddy food. Methods: This study investigated differential components between organic and regular milk by stable isotope ratio and UPLC-Q-Orbitrap-based metabolomics. Organic milk with different lactation stages was also analyzed. Results: There was a significant difference between organic and ordinary milk for single factor ANOVA P<0.01. The carbon stable isotope and nitrogen stable isotope ratios could be used to distinguish organic and ordinary milk. Based on the data from UPLC-Q-Orbitrap, differential metabolites were screened by the OPLS-DA method, five different constituents were obtained, and there was a big difference between different lactation stages of organic milk such as histidine etc. Conclusion: Although the data of this study was obtained from limited samples, the different constituents between organic and ordinary milk were confirmed. Organic milk could be preliminarily determined by the data of carbon isotope ratio, nitrogen isotope ratio, and five different constituents screened by the UPLC-Q-Orbitrap method. This study could provide a scientific basis for the identification of organic and ordinary milk.
- organic milk,
- carbon isotope ratio and nitrogen isotope ratio,
- UPLC-Q-Orbitrap,
- different constituents

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References

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Science and Technology of Food Industry

Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

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